Food to fight hidden hunger

Wednesday, Jan 22, 2020, 01:02 AM | Source: Pursuit

Alex Johnson

As far as relationships go, the human love affair with bread is a long one.

The oldest evidence for bread making comes from the remains of a 14,400 year-old flatbread recently discovered in north-eastern Jordan.

Anthropologists believe that hunter-gatherers made the bread using wild grains, nearly 4,000 years before Neolithic farmers started growing what we now know of as wheat.

Almost every country and culture has developed an iconic bread. Picture: Getty Images

It may even be that bread making inspired early humans to begin cultivating crops.

Since then, almost every country and culture has developed an iconic bread, from the dark and dense German pumpernickel and the crusty French baguette, to the flat Indian chapati, just to name a few.

But as with all long relationships, this one is complex.


For billions of people around the world, cereal-based foods including bread, rice and noodles provide a significant proportion of daily calories and energy.

However, many of these foods are poor sources of essential micronutrients like iron and zinc.

Plant biologist Associate Professor Alex Johnson explains that high cereal consumption, particularly in developing countries, contributes towards an estimated two billion people worldwide suffering from iron and zinc deficiencies.

Nutritionists describe a lack of essential vitamins and minerals in the human diet as ‘hidden hunger’.

“The symptoms of hidden hunger may not be as visible as a lack of calories, but the consequences for human health and productivity are devastating,” says Associate Professor Johnson, from the University of Melbourne’s School of BioSciences.

WATCH: Alex Johnson and his team are working to boost the levels of essential micronutrients in wheat. Video: University of Melbourne

Iron deficiency can cause people to develop anaemia, making them more susceptible to disease and posing potentially fatal health risks, especially for women and children.

Low levels of zinc also have serious health consequences including reduced immune function and childhood stunting.

To help tackle hidden hunger, Associate Professor Johnson and his team are working to boost the levels of essential micronutrients in wheat and other staple crops – a technique known as ‘biofortification’.

“Biofortification represents a sustainable solution to hidden hunger because you’re increasing nutritional quality of the crop itself, as farmers share biofortified seeds they amplify the health benefits,” says Associate Professor Johnson.


Despite iron being an abundant element in Earth’s soils, it is very difficult for plants to absorb and most species have low iron levels in seeds, which are the parts that we frequently eat.

Associate Professor Johnson explains that this poses major challenges when trying to breed iron-enriched cereals.

“Decades of conventional breeding haven’t been able to produce iron-biofortified wheat or rice. Even in cases where iron levels have increased, they’re often small increases and in tissues of the plant that we can’t eat or easily digest”.

The research team in the field: From left, Rucha Patil (MSc BioSciences student), Associate Professor Alex Johnson, Dr Julien Bonneau (postdoctoral researcher), Oscar Fung (MSc BioSciences student) and Ouda Khammy (laboratory supervisor). Picture: University of Melbourne

To overcome the hurdles facing iron biofortification, Associate Professor Johnson’s research team has utilised plant biotechnology.

Their research effort began approximately a decade ago when the team identified a subset of genes in rice that are ‘switched on’ when the plant senses it is low in iron. They next developed a way to keep one of these genes ‘always on’ and, in doing so, created iron and zinc biofortified rice.

In results recently published in the Plant Biotechnology Journal, they have now shown that this approach works equally well in wheat. Using the same rice gene, the team employed genetic engineering to shuttle the gene into wheat and leave it ‘always on’.

The work resulted in biofortified wheat plants with 69 parts per million (ppm) iron in the grain – nearly two-fold higher than conventional grain (38 ppm) and well above the 52 ppm iron target needed to adequately biofortify wheat grain for nutritional benefit.

Furthermore, a significant proportion of the biofortified iron was localised in the inner grain tissues that are used to make white flour, resulting in up to 7 ppm more iron in the biofortified flour.

“This is probably the best result we’ve obtained yet”, he says.

“The extra iron is deep within the grain, and it stays there even when we mill the grain to make white flour”.

Synchrotron X‐ray fluorescence microscopy images of conventional (left column) and biofortified (right column) wheat grain cross-sections. Biofortified grain has more Fe in the inner endosperm tissue and more Zn in the folded crease. Colour bar represents high (white) and low (black) concentrations of and Iron (Fe) and Zinc (Zn). Picture: Plant Biotechnology Journal/ Wiley Publishing

As an added benefit, more zinc accumulated in a region of the grain that cannot be removed by milling, called the crease, meaning that the white flour also contained more zinc.

While boosting iron and zinc concentrations in wheat is a major accomplishment by itself, another challenge is ensuring that these micronutrients are in forms that our bodies can absorb and use – that is, they are ‘bioavailable’.

“It’s like money. You can have all the money you need in the bank but if you can’t access it, you may as well have none,” says Associate Professor Johnson.

The team investigated iron bioavailability by pre-digesting flour in a similar way to the stomach and ‘feeding’ it to Caco-2 cells – a laboratory cell line that mimics the human small intestine.

They found that iron from biofortified white flour entered Caco-2 cells at nearly double the rate of conventional white flour.

“These results indicate that we’re not only enriching wheat with more iron, but also more digestible forms of iron”.


Alex Johnson’s team is now collaborating with researchers at the National Institute for Biotechnology and Genetic Engineering (NIBGE) in Faisalabad, Pakistan, and aims to begin field trials of biofortified wheat in Pakistan this year.

With a population of more than 200 million people and high rates of hidden hunger, Pakistan is a country that could quickly reap the benefits of biofortified wheat.

First field trial of iron and zinc biofortified wheat at the University of Melbourne’s Dookie campus. Picture: University of Melbourne

Closer to home, the biofortified wheat has just undergone its first field trial at the University of Melbourne’s Dookie campus near Shepparton in Victoria.

The biofortified plants yielded just as well as conventional wheat, in some cases even better, and the team is now using the harvested grain to bake and analyse a range of iron- and zinc-biofortified breads.

“Many researchers, including those in my team, are in the process of reimagining our love affair with bread,” Associate Professor Johnson says.

“An important part of that is coupling bread’s delicious taste with enhanced nutritional value”.

Banner: Getty Images

University of Melbourne Researchers