Carolyn de Graaf

In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis.

C Biben, TS Weber, KS Potts, J Choi, DC Miles, A Carmagnac, T Sargeant, CA de Graaf, KA Fennell, A Farley, OJ Stonehouse, MA Dawson, DJ Hilton, SH Naik, S Taoudi

Journal article  |  2023  |  Nat Commun

During embryogenesis, haematopoietic and endothelial lineages emerge closely in time and space. It is thought that the first blood..

What can we learn from mice lacking pro-survival BCL-2 proteins to advance BH3 mimetic drugs for cancer therapy?

Kerstin Brinkmann, Ashley P Ng, Carolyn A de Graaf, Andreas Strasser

Journal article  |  2022  |  CELL DEATH AND DIFFERENTIATION

In many human cancers the control of apoptosis is dysregulated, for instance as a result of the overexpression of pro-survival BCL..

Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent antitumor immunity in melanoma

Nicolas Jacquelot, Cyril Seillet, Minyu Wang, Angela Pizzolla, Yang Liao, Soroor Hediyeh-zadeh, Sharon Grisaru-Tal, Cynthia Louis, Qiutong Huang, Jaring Schreuder, Fernando Souza-Fonseca-Guimaraes, Carolyn A de Graaf, Kevin Thia, Sean Macdonald, Mary Camilleri, Kylie Luong, Shengbo Zhang, Michael Chopin, Tristan Molden-Hauer, Stephen L Nutt, et al.

Journal article  |  2021  |  NATURE IMMUNOLOGY

Group 2 innate lymphoid cells (ILC2s) are essential to maintain tissue homeostasis. In cancer, ILC2s can harbor both pro-tumorigen..

Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes

Christine R Keenan, Michael J Mlodzianoski, Hannah D Coughlan, Naiara G Bediaga, Gaetano Naselli, Erin C Lucas, Qike Wang, Carolyn A de Graaf, Douglas J Hilton, Leonard C Harrison, Gordon K Smyth, Kelly L Rogers, Thomas Boudier, Rhys S Allan, Timothy M Johanson

Journal article  |  2021  |  ISCIENCE

The proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respect..

A new lymphoid-primed progenitor marked byDach1downregulation identified with single cell multi-omics

Daniela Amann-Zalcenstein, Luyi Tian, Jaring Schreuder, Sara Tomei, Dawn S Lin, Kirsten A Fairfax, Jessica E Bolden, Mark D McKenzie, Andrew Jarratt, Adrienne Hilton, Jacob T Jackson, Ladina Di Rago, Matthew P McCormack, Carolyn A de Graaf, Olivia Stonehouse, Samir Taoudi, Warren S Alexander, Stephen L Nutt, Matthew E Ritchie, Ashley P Ng, et al.

Journal article  |  2020  |  NATURE IMMUNOLOGY

A classical view of blood cell development is that multipotent hematopoietic stem and progenitor cells (HSPCs) become lineage-rest..

Membrane budding is a major mechanism of in vivo platelet biogenesis

Kathryn S Potts, Alison Farley, Caleb A Dawson, Joel Rimes, Christine Biben, Carolyn de Graaf, Margaret A Potts, Olivia J Stonehouse, Amandine Carmagnac, Pradnya Gangatirkar, Emma C Josefsson, Casey Anttila, Daniela Amann-Zalcenstein, Shalin Naik, Warren S Alexander, Douglas J Hilton, Edwin D Hawkins, Samir Taoudi

Journal article  |  2020  |  JOURNAL OF EXPERIMENTAL MEDICINE

How platelets are produced by megakaryocytes in vivo remains controversial despite more than a century of investigation. Megakaryo..

A missense mutation in the MLKL brace region promotes lethal neonatal inflammation and hematopoietic dysfunction

Joanne M Hildebrand, Maria Kauppi, Ian J Majewski, Zikou Liu, Allison J Cox, Sanae Miyake, Emma J Petrie, Michael A Silk, Zhixiu Li, Maria C Tanzer, Gabriela Brumatti, Samuel N Young, Cathrine Hall, Sarah E Garnish, Jason Corbin, Michael D Stutz, Ladina Di Rago, Pradnya Gangatirkar, Emma C Josefsson, Kristin Rigbye, et al.

Journal article  |  2020  |  NATURE COMMUNICATIONS

MLKL is the essential effector of necroptosis, a form of programmed lytic cell death. We have isolated a mouse strain with a singl..

miR17~92 restrains pro-apoptotic BIM to ensure survival of haematopoietic stem and progenitor cells

Kerstin Brinkmann, Ashley P Ng, Carolyn A de Graaf, Ladina Di Rago, Craig D Hyland, Eugenio Morelli, Jai Rautela, Nicholas D Huntington, Andreas Strasser, Warren S Alexander, Marco J Herold

Journal article  |  2020  |  CELL DEATH AND DIFFERENTIATION

The miR17~92 cluster plays important roles in haematopoiesis. However, it is not clear at what stage of differentiation and throug..

Haemopedia RNA-seq: a database of gene expression during haematopoiesis in mice and humans

Jarny Choi, Tracey M Baldwin, Mae Wong, Jessica E Bolden, Kirsten A Fairfax, Erin C Lucas, Rebecca Cole, Christine Biben, Clare Morgan, Kerry A Ramsay, Ashley P Ng, Maria Kauppi, Lynn M Corcoran, Wei Shi, Nick Wilson, Michael J Wilson, Warren S Alexander, Douglas J Hilton, Carolyn A de Graaf

Journal article  |  2019  |  NUCLEIC ACIDS RESEARCH

During haematopoiesis, haematopoietic stem cells differentiate into restricted potential progenitors before maturing into the many..

Plasmacytoid dendritic cell heterogeneity is defined by CXCL10 expression following TLR7 stimulation

Casper Marsman, Fanny Lafouresse, Yang Liao, Tracey M Baldwin, Lisa A Mielke, Yifang Hu, Matthias Mack, Paul J Hertzog, Carolyn A de Graaf, Wei Shi, Joanna R Groom

Journal article  |  2018  |  IMMUNOLOGY AND CELL BIOLOGY

Plasmacytoid dendritic cells (pDCs) play a critical role in bridging the innate and adaptive immune systems. pDCs are specialized ..