Elizabeth Hinde

A Molecular Chameleon for Mapping Subcellular Polarity in an Unfolded Proteome Environment

Tze Cin Owyong, Pramod Subedi, Jieru Deng, Elizabeth Hinde, Jason J Paxman, Jonathan M White, Weisan Chen, Begona Heras, Wallace WH Wong, Yuning Hong

Journal article  |  2020  |  Angewandte Chemie International Edition

Environmental polarity is an important factor that drives biomolecular interactions to regulate cell function. Herein, a general m..

Quantifying nuclear wide chromatin compaction by phasor analysis of histone Forster resonance energy transfer (FRET) in frequency domain fluorescence lifetime imaging microscopy (FLIM) data

Zhen Liang, Jieqiong Lou, Lorenzo Scipioni, Enrico Gratton, Elizabeth Hinde

Journal article  |  2020  |  Data in Brief

Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.

Sunyuan Zhang, Elizabeth Hinde, Molly Parkyn Schneider, David A Jans, Marie A Bogoyevitch

Journal article  |  2020  |  Scientific Reports

A mutant form of the ataxin-1 protein with an expanded polyglutamine (polyQ) tract is the underlying cause of the inherited neurod..

Fluorescence fluctuation spectroscopy: an invaluable microscopy tool for uncovering the biophysical rules for navigating the nuclear landscape

David G Priest, Ashleigh Solana, Jieqiong Lou, Elizabeth Hinde

Journal article  |  2019  |  Biochemical Society Transactions

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response

Jieqiong Lou, Lorenzo Scipioni, Belinda K Wright, Tara K Bartolec, Jessie Zhang, V Pragathi Masamsetti, Katharina Gaus, Enrico Gratton, Anthony J Cesare, Elizabeth Hinde

Journal article  |  2019  |  Proceedings of the National Academy of Sciences

Ponatinib Inhibits Multiple Signaling Pathways Involved in STAT3 Signaling and Attenuates Colorectal Tumor Growth

Fiona H Tan, Tracy L Putoczki, Jieqiong Lou, Elizabeth Hinde, Frederic Hollande, Julie Giraud, Stanley S Stylli, Lucia Paradiso, Hong-Jian Zhu, Oliver M Sieber, Rodney B Luwor

Journal article  |  2018  |  CANCERS

Pair correlation microscopy reveals the role of nanoparticle shape in intracellular transport and site of drug release.

Elizabeth Hinde, Kitiphume Thammasiraphop, Hien TT Duong, Jonathan Yeow, Bunyamin Karagoz, Cyrille Boyer, J Justin Gooding, Katharina Gaus

Journal article  |  2017  |  Nature Nanotechnology

Nanoparticle size, surface charge and material composition are known to affect the uptake of nanoparticles by cells. However, whet..

Dextran-Catechin: An anticancer chemically-modified natural compound targeting copper that attenuates neuroblastoma growth.

Orazio Vittorio, Miriam Brandl, Giuseppe Cirillo, Kathleen Kimpton, Elizabeth Hinde, Katharina Gaus, Eugene Yee, Naresh Kumar, Hien Duong, Claudia Fleming, Michelle Haber, Murray Norris, Cyrille Boyer, Maria Kavallaris

Journal article  |  2016  |  Oncotarget

Neuroblastoma is frequently diagnosed at advanced stage disease and treatment includes high dose chemotherapy and surgery. Despite..

Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy.

Natalie M Clark, Elizabeth Hinde, Cara M Winter, Adam P Fisher, Giuseppe Crosti, Ikram Blilou, Enrico Gratton, Philip N Benfey, Rosangela Sozzani

Journal article  |  2016  |  eLife

To understand complex regulatory processes in multicellular organisms, it is critical to be able to quantitatively analyze protein..

Distinct mechanisms regulate Lck spatial organization in activated T cells

N Kapoor-Kaushik, E Hinde, EB Compeer, Y Yamamoto, F Kraus, Z Yang, J Lou, SV Pageon, T Tabarin, K Gaus, J Rossy

Journal article  |  2016  |  Frontiers in Immunology

Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness

Elizabeth Hinde, Elvis Pandzic, Zhengmin Yang, Ivan HW Ng, David A Jans, Marie A Bogoyevitch, Enrico Gratton, Katharina Gaus

Journal article  |  2016  |  Nature Communications

Dynamic Organization of SecA and SecY Secretion Complexes in the B. subtilis Membrane.

Alex Dajkovic, Elizabeth Hinde, Calum MacKichan, Rut Carballido-Lopez

Journal article  |  2016  |  PLOS ONE

In prokaryotes, about one third of cellular proteins are translocated across the plasma membrane or inserted into it by concerted ..

Enhancing Quantum Dots for Bioimaging using Advanced Surface Chemistry and Advanced Optical Microscopy: Application to Silicon Quantum Dots (SiQDs).

Xiaoyu Cheng, Elizabeth Hinde, Dylan M Owen, Stuart B Lowe, Peter J Reece, Katharina Gaus, J Justin Gooding

Journal article  |  2015  |  Advanced Materials

Fluorescence lifetime imaging microscopy is successfully demonstrated in both one- and two-photon cases with surface modified, nan..

Spatiotemporal regulation of Heterochromatin Protein 1-alpha oligomerization and dynamics in live cells.

Elizabeth Hinde, Francesco Cardarelli, Enrico Gratton

Journal article  |  2015  |  Scientific Reports

Heterochromatin protein 1 (HP1) is a central factor in establishing and maintaining the heterochromatin state. As consequence of p..

Graphene oxide - gelatin nanohybrids as functional tools for enhanced Carboplatin activity in neuroblastoma cells.

Sami Makharza, Orazio Vittorio, Giuseppe Cirillo, Steffen Oswald, Elizabeth Hinde, Maria Kavallaris, Bernd Büchner, Michael Mertig, Silke Hampel

Journal article  |  2015  |  Pharmaceutical Research

PURPOSE: Preparation of Nanographene oxide (NGO) - Gelatin hybrids for efficient treatment of Neuroblastoma. METHODS: Nanohybrids ..

Nanodomains in biological membranes.

Yuanqing Ma, Elizabeth Hinde, Katharina Gaus

Journal article  |  2015  |  Essays in Biochemistry

Lipid rafts are defined as cholesterol- and sphingomyelin-enriched membrane domains in the plasma membrane of cells that are highl..

Spatio-Temporal Regulation of Rac1 Mobility by Actin Islands.

Vinal V Lakhani, Elizabeth Hinde, Enrico Gratton, Timothy C Elston

Journal article  |  2015  |  PLOS ONE

Rho GTPases play important roles in many aspects of cell migration, including polarity establishment and organizing actin cytoskel..

The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells.

Ottavia Golfetto, Elizabeth Hinde, Enrico Gratton

Book Chapter  |  2015  |  Methods in Molecular Biology

In this method paper we describe the spectral phasor analysis applied to Laurdan emission for the assessment of the fluidity of di..

Microscopy approaches to investigate protein dynamics and lipid organization.

Joanna M Kwiatek, Elizabeth Hinde, Katharina Gaus

Journal article  |  2014  |  Molecular Membrane Biology

The structure of cell membranes has been intensively investigated and many models and concepts have been proposed for the lateral ..

Chromatin dynamics during DNA repair revealed by pair correlation analysis of molecular flow in the nucleus.

Elizabeth Hinde, Xiangduo Kong, Kyoko Yokomori, Enrico Gratton

Journal article  |  2014  |  Biophysical Journal

Chromatin dynamics modulate DNA repair factor accessibility throughout the DNA damage response. The spatiotemporal scale upon whic..

Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation.

Elizabeth Hinde, Kyoko Yokomori, Katharina Gaus, Klaus M Hahn, Enrico Gratton

Journal article  |  2014  |  Sci Rep

Here we describe a fluctuation-based method to quantify how protein oligomerisation modulates signalling activity of a multifuncti..

Effects of light irradiance on stomatal regulation and growth of tomato

A O'Carrigan, E Hinde, N Lu, XQ Xu, H Duan, G Huang, M Mak, B Bellotti, ZH Chen

Journal article  |  2014  |  Environmental and Experimental Botany

Fluid shear stress on endothelial cells modulates mechanical tension across VE-cadherin and PECAM-1.

Daniel E Conway, Mark T Breckenridge, Elizabeth Hinde, Enrico Gratton, Christopher S Chen, Martin A Schwartz

Journal article  |  2013  |  Current Biology

Fluid shear stress (FSS) from blood flow acting on the endothelium critically regulates vascular morphogenesis, blood pressure, an..

FLUORIMETRIC ANALYSIS OF THE CONSTITUENT DYES WITHIN DAYLIGHT FLUORESCENT PIGMENTS: IMPLICATIONS FOR DISPLAY AND PRESERVATION OF DAYLIGHT FLUORESCENT ARTWORK

Elizabeth Hinde, Petronella Nel, Robyn Sloggett, Ann Roberts

Journal article  |  2013  |  Journal of the American Institute for Conservation

Laurdan fluorescence lifetime discriminates cholesterol content from changes in fluidity in living cell membranes.

Ottavia Golfetto, Elizabeth Hinde, Enrico Gratton

Journal article  |  2013  |  Biophysical Journal

Detection of the fluorescent properties of Laurdan has been proven to be an efficient tool to investigate membrane packing and ord..

Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.

Elizabeth Hinde, Michelle A Digman, Klaus M Hahn, Enrico Gratton

Journal article  |  2013  |  Proceedings of the National Academy of Sciences

Here we present a fluctuation-based approach to biosensor Förster resonance energy transfer (FRET) detection that can measure the ..

Tracking the mechanical dynamics of human embryonic stem cell chromatin

E Hinde, F Cardarelli, A Chen, M Khine, E Gratton

Journal article  |  2012  |  Epigenetics & Chromatin

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy.

Elizabeth Hinde, Michelle A Digman, Christopher Welch, Klaus M Hahn, Enrico Gratton

Journal article  |  2012  |  Microscopy Research and Technique

We present here the phasor approach to biosensor Förster resonance energy transfer (FRET) detection by fluorescence lifetime imagi..

Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus.

Elizabeth Hinde, Francesco Cardarelli, Michelle A Digman, Enrico Gratton

Journal article  |  2012  |  Biophysical Journal

We present a quantitative fluctuation-based assay to measure the degree of local chromatin compaction and investigate how chromati..

Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK.

Ryan L Stafford, Elizabeth Hinde, Mary Jane Knight, Mario A Pennella, Jason Ear, Michelle A Digman, Enrico Gratton, James U Bowie

Journal article  |  2011  |  Structure

The synaptic scaffolding proteins CASK and Caskin1 are part of the fibrous mesh of proteins that organize the active zones of neur..

Measuring the flow of molecules in cells

E Hinde, F Cardarelli

Journal article  |  2011  |  Biophysical Reviews

The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis.

Elizabeth Hinde, Francesco Cardarelli, Michelle A Digman, Aaron Kershner, Judith Kimble, Enrico Gratton

Journal article  |  2011  |  Biophysical Journal

Here we address the impact nuclear architecture has on molecular flow within the mitotic nucleus of live cells as compared to inte..

In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow.

Elizabeth Hinde, Francesco Cardarelli, Michelle A Digman, Enrico Gratton

Journal article  |  2010  |  Proceedings of the National Academy of Sciences

No methods proposed thus far have the capability to measure overall molecular flow in the nucleus of living cells. Here, we apply ..

A novel technique for the photography of daylight fluorescent artwork

P NEL, E HINDE

Conference Proceedings  |  2008  |  ICOM Committee for Conservation 15th Triennial Conference New Delhi