Anthony Wedd

The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family

Saumya R Udagedara, Chathuri JK Wijekoon, Zhiguang Xiao, Anthony G Wedd, Megan J Maher

Journal article  |  2019  |  JOURNAL OF INORGANIC BIOCHEMISTRY

The bacterial CopC family of proteins are periplasmic copper binding proteins that act in copper detoxification. These proteins co..

Molecular Mechanisms of Glutaredoxin Enzymes: Versatile Hubs for Thiol-Disulfide Exchange between Protein Thiols and Glutathione

Zhiguang Xiao, Sharon La Fontaine, Ashley I Bush, Anthony G Wedd

Journal article  |  2019  |  JOURNAL OF MOLECULAR BIOLOGY

The tripeptide glutathione (GSH) and its oxidized form glutathione disulfide (GSSG) constitute a key redox couple in cells. In par..

The Human Amyloid Precursor Protein Binds Copper Ions Dominated by a Picomolar-Affinity Site in the Helix-Rich E2 Domain

Tessa R Young, Tara L Pukala, Roberto Cappai, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  BIOCHEMISTRY

A manifestation of Alzheimer's disease (AD) is the aggregation in the brain of amyloid β (Aβ) peptides derived from the amyloid pr..

Glutaredoxins employ parallel monothiol-dithiol mechanisms to catalyze thiol-disulfide exchanges with protein disulfides

Ashwinie A Ukuwela, Ashley I Bush, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  CHEMICAL SCIENCE

Glutaredoxins (Grxs) are a family of glutathione (GSH)-dependent thiol-disulfide oxidoreductases. They feature GSH-binding sites t..

C-F bond activation of trifluoroethanol and trifluoroacetic acid catalysed by the dimolybdate anion, [Mo2O6(F)](-)

George N Khairallah, Tom Waters, Anthony G Wedd, Richard AJ O'Hair

Journal article  |  2018  |  EUROPEAN JOURNAL OF MASS SPECTROMETRY

Two gas-phase catalytic cycles involving C-F bond activation of trifluoroethanol and trifluoroacetic acid were detected by multist..

Evaluation of Cu(I) binding to the E2 domain of the amyloid precursor protein - a lesson in quantification of metal binding to proteins via ligand competition

Tessa R Young, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  METALLOMICS

The extracellular domain E2 of the amyloid precursor protein (APP) features a His-rich metal-binding site (denoted as the M1 site)..

Reduction potentials of protein disulfides and catalysis of glutathionylation and deglutathionylation by glutaredoxin enzymes

Ashwinie A Ukuwela, Ashley I Bush, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2017  |  BIOCHEMICAL JOURNAL

Glutaredoxins (Grxs) are a class of GSH (glutathione)-dependent thiol-disulfide oxidoreductase enzymes. They use the cellular redo..

Molecular Aspects of a Robust Assay for Ferroxidase Function of Ceruloplasmin

Laura Cortes, Blaine R Roberts, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2017  |  INORGANIC CHEMISTRY

Ceruloplasmin (Cp) is one of the most complex multicopper oxidase enzymes and plays an essential role in the metabolism of iron in..

Copper ATPase CopA from Escherichia coli: Quantitative Correlation between ATPase Activity and Vectorial Copper Transport

Chathuri JK Wijekoon, Saumya R Udagedara, Roland L Knorr, Rumiana Dimova, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2017  |  Journal of the American Chemical Society

Cu-ATPases are membrane copper transporters present in all kingdoms of life. They play a central role in Cu homeostasis by pumping..

Copper binding and redox chemistry of the A beta 16 peptide and its variants: insights into determinants of copper-dependent reactivity

Nineveh Yako, Tessa R Young, Jade M Cottam Jones, Craig A Hutton, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2017  |  METALLOMICS

The metal-binding sites of Aβ peptides are dictated primarily by the coordination preferences of the metal ion. Consequently, Cu(i..