Prof Neil O'Brien-Simpson

Neil O’Brien-Simpson has an interdisciplinary background, combining organic and peptide chemistry with microbiology and immunology to develop novel vaccines and therapeutics and investigating the immune response to pathogens. Thus his field of research is in chemical biology/immunology and host/pathogen (bacteria/fungi) mucosal interaction.

He was originally trained in biological sciences and management at Edinburgh Napier University (Edinburgh, Scotland) and worked at I.C.I Chemicals and Polymers Division (Grangemouth and Runcorn) as a research organic chemist. In 1993 he was awarded an Overseas postgraduate research scholarship and a CRC for vaccine technology scholarship to study for his Ph.D at The University of Melbourne, Department of Microbiology and Immunology. During his Ph.D he was trained in peptide chemistry and immunology and developed in his thesis a method of producing a multi-valent peptide vaccine, which resulted in several publications and a patent. After being awarded his Ph.D he went to the Melbourne Dental School, The University of Melbourne, to do postdoctoral research with Eric Reynolds on the development of a vaccine against the bacterium Porphyromonas gingivalis a causative agent of periodontitis. At the Melbourne Dental School A/Prof O’Brien-Simpson has established a peptide/organic chemistry laboratory and a microbial-immunology laboratory to conduct research into AMPs, vaccines and bacterial immunity. He was part of a team in 2003 and 2009 that was awarded a Co-operative Research Centre grant for establishing the CRC for Oral Health Science and Oral Health CRC, respectively served as a Program Leader (2003-2009) and currently as a Project Manager for vaccine design and therapeutic development.

Find out more about Neil O'Brien-Simpson's experience

Neil O'Brien-Simpson's highlights

FEATURED Journal article

Keratinocyte-specific ablation of protease-activated receptor 2 prevents gingival inflammation and bone loss in a mouse model of periodontal disease

Neil O'Brien-Simpson's selected work

Reversing Antibiotic Resistance In Superbugs

Research Grant

Metabolic Remodeling, Inflammasome Activation, and Pyroptosis in Macrophages Stimulated by..

Journal article

Designing Antimicrobial Peptides To Target Pathogenic Bacteria Associated With Periodontit..

Research Grant

The 9-Fluorenylmethoxycarbonyl (Fmoc) group in chemical peptide synthesis-its past, presen..

Journal article

Proline-Rich Host Defense Peptide Analogues As New Antibiotics Against Mdr Bacteria

Research Grant

Comparison of the effectiveness of a tailored cognitive behavioural therapy with a support..

Journal article

Projects

Displaying the 26 most recent projects by Neil O'Brien-Simpson.

Reversing Antibiotic Resistance In Superbugs

2020 - 2023

Active

Designing Antimicrobial Peptides To Target Pathogenic Bacteria Associated With Periodontitis

2019 - 2020

Active

Proline-Rich Host Defense Peptide Analogues As New Antibiotics Against Mdr Bacteria

2019 - 2022

Active

Calcium Phosphate Nanoparticles As Mucosal Cancer Vaccine Adjuvants

2019 - 2021

Active

Human Pbmc Cell Type Responses To Periodontal Pathogen Outer Membrane Vesicles.

2019 - 2019

Completed

Star Polymers As Novel Antimicrobial And Immunomodulatory Agents

The rise in antibiotic resistance in bacteria is considered as a major public health threat that is not being met by antibiotic research. Th..

2018 - 2021

Active

Star Polymers As Novel Antimicrobial And Immunomodulatory Agents

The rise in antibiotic resistance in bacteria is considered as a major public health threat that is not being met by antibiotic research. Th..

2018 - 2021

Active

Antimicrobial Peptides That Target Periodontal Pathogens

2018 - 2020

Active

Single Cell Mass Cytometer Research Initiatives Fund Collaborative Equipment Grant

2017 - 2018

Completed

The Role Of Porphyromonas Gingivalis Outer Membrane Vesicle Biogenesis In Virulence And Immunomodulation

Porphyromonas gingivalis is a keystone pathogen in chronic periodontitis. This bacterium exists as part of a biofilm on the surface of the t..

2016 - 2019

Completed

Structurally Nanoengineered Antimicrobial Polypeptide Particles (Snapps)

This project aims to develop a new platform technology for the development of antimicrobial agents by combining expertise in polymer science..

2016 - 2020

Active

Structurally Nanoengineered Antimicrobial Polypeptide Particles (Snapps)

This project aims to develop a new platform technology for the development of antimicrobial agents by combining expertise in polymer science..

2016 - 2020

Active

Role Of T Cell In Chronic Periodontitis

Particular immune cell populations (innate T cells), have a critical role in immunity. These cells are stimulated by bacterial cell wall com..

2012 - 2014

Completed

A Cross-Sectional Study Investigating The Association Between Oral Bacteria And Jaw Osteonecrosis In Subjects Using Intravenous Bisphosphonates For Malignancy

2012 - 2012

Completed

Triple-Antigen Vaccine For Chronic Periodontitis

Periodontitis is a chronic inflammatory disease associated with specific bacteria leading to the destruction of the toothÍs supporting tissu..

2012 - 2014

Completed

Development Of Antimicrobial Peptides Targeting Oral Pathogenic Bacteria

The bacterial associated oral diseases; periodontitis and caries are major public health problems. The prevalence of these diseases and incr..

2011 - 2013

Completed

The Interaction Of P.Gingivalis With Lymphocyte Subsets And Myeloid Cells

2011 - 2011

Completed

The Synthesis Of Multiphosphorylated Analogues Of The Anticariogenic Casein Phosphopeptides And Their Calcium Phosphate-Binding Potentials

2011 - 2011

Completed

Development Of A Vaccine To Porphyromonas Gingivalis

Chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with specific bacteria, which results in ..

2009 - 2011

Completed

Development Of A Synthetic Peptide Vaccine

Chronic periodontitis is a bacterial-associated inflammatory disease of the supporting tissues of the teeth, which results in the destructio..

2007 - 2009

Completed

Nanoparticle Mucosal Vaccines

Polymicrobial Pathogenesis In A Murine Periodontitis Model.

Severe periodontal disease is a chronic inflammatory disease of the tooth's supporting tissues and is associated with specific pathogenic ba..

Development Of A Site-Specific, Predictive Assay For Periodontal Disease Progression Using Mass Spectrometric And Real Time Pcr Analyses

In this project new DNA-based technology will be developed to determine the numbers of specific bacteria in the dental plaque of patients. T..

Molecular Studies Of Dentine Phosphophoryn And Development Of A Biomimetic Dental Restorative Material.

This project involves the study of a protein that is found in teeth and is responsible for the development of dentine. The project involves ..

Project Agreement - Animal Models Of Disease And Pharmaceutical Development - Investigation Of The Tlr Response Pathway

Development Of A Defined Porphyromonas Gingivalis Vaccine

26

Projects

Project Types

Research Grant

Internal Research Grant

Research Contracts

Scholarly Works

Displaying the 141 most recent scholarly works by Neil O'Brien-Simpson.

The 9-Fluorenylmethoxycarbonyl (Fmoc) group in chemical peptide synthesis-its past, present, and future

W Li, NM O'Brien-Simpson, MA Hossain, JD Wade

Journal article | 2020 | Australian Journal of Chemistry

The chemical formation of the peptide bond has long fascinated and challenged organic chemists. It requires not only the activatio..

Comparison of the effectiveness of a tailored cognitive behavioural therapy with a supportive listening intervention for depression in those newly diagnosed with multiple sclerosis (the ACTION-MS trial): protocol of an assessor-blinded, active comparator, randomised controlled trial (vol 21, 100, 2020)

Litza Kiropoulos, Trevor Kilpatrick, Tomas Kalincik, Leonid Churilov, Elizabeth McDonald, Tissa Wijeratne, Jennifer Threader, Vanja Rozenblat, Neil O'Brien-Simpson, Anneke Van Der Walt, Lisa Taylor

Journal article | 2020 | Trials

After publication of our article [1] the authors have notified us that three of the names have been incorrectly spelled.

Celogentin mimetics as inhibitors of tubulin polymerization

Varsha J Thombare, James A Holden, Eric C Reynolds, Neil M O'Brien-Simpson, Craig A Hutton

Journal article | 2020 | Journal of Peptide Science

Bicyclic analogues of celogentin C have been synthesized in which the side chain-side chain cross-links are replaced by thioether ..

Comparison of the effectiveness of a tailored cognitive behavioural therapy with a supportive listening intervention for depression in those newly diagnosed with multiple sclerosis (the ACTION-MS trial): protocol of an assessor-blinded, active comparator, randomised controlled trial

Litza Kiropoulos, Trevor Kilpatrick, Tomas Kalincek, Leonid Cherulov, Elizabeth McDonald, Tissa Wijeratne, Jennifer Threader, Vanja Rozenblat, Neil Simpson-O'Brien, Anneke Van der Walt, Lisa Taylor

Journal article | 2020 | Trials

BACKGROUND: Multiple sclerosis (MS) is an unpredictable, chronic neurological disease accompanied with high rates of depression an..

Chemical Modification of Cellulose Membranes for SPOT Synthesis

Wenyi Li, John Wade, Eric Reynolds, Neil M O'Brien-Simpson

Journal article | 2020 | Australian Journal of Chemistry

Since the development of solid-phase peptide synthesis in the 1960s, many laboratories have modified the technology for the produc..

Engineering highly effective antimicrobial selenium nanoparticles through control of particle size

Tao Huang, James A Holden, Daniel E Heath, Neil M O'Brien-Simpson, Andrea J O'Connor

Journal article | 2019 | Nanoscale

The overuse of antibiotics has induced the rapid development of antibiotic resistance in bacteria. As a result, antibiotic efficac..

Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: in vitro and in vivo assessment

Phong Atran, Neil O'Brien-Simpson, Jason A Palmer, Nathalie Bock, Eric C Reynolds, Thomas J Webster, Anand Deva, Wayne A Morrison, Andrea J O'Connor

Journal article | 2019 | International Journal of Nanomedicine

Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which is o..

Localization of Outer Membrane Proteins in Treponema denticola by Quantitative Proteome Analyses of Outer Membrane Vesicles and Cellular Fractions

Paul D Veith, Michelle D Glew, Dhana G Gorasia, Dina Chen, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2019 | Journal of Proteome Research

Combating bacterial resistance by combination of antibiotics with antimicrobial peptides

Dean E Sheard, Neil M O'Brien-Simpson, John D Wade, Frances Separovic

Journal article | 2019 | Pure and Applied Chemistry

Outer Membrane Vesicle-Host Cell Interactions

Jessica D Cecil, Natalie Sirisaengtaksin, Neil M O'Brien-Simpson, Anne Marie Krachler

Journal article | 2019 | Microbiology Spectrum

Monospecies and polymicrobial biofilms differentially regulate the phenotype of genotype-specific oral cancer cells

Mohd Hafiz Arzmi, Nicola Cirillo, Jason C Lenzo, Deanne V Catmull, Neil O'Brien-Simpson, Eric C Reynolds, Stuart Dashper, Michael McCullough

Journal article | 2019 | Carcinogenesis

Architectural Effects of Star-Shaped "Structurally Nanoengineered Antimicrobial Peptide Polymers" (SNAPPs) on Their Biological Activity

Steven J Shirbin, Ignacio Insua, James A Holden, Jason C Lenzo, Eric C Reynolds, Neil M O'Brien-Simpson, Greg G Qiao

Journal article | 2018 | Advanced Healthcare Materials

Keratinocyte-specific ablation of protease-activated receptor 2 prevents gingival inflammation and bone loss in a mouse model of periodontal disease

Nidhish Francis, Babatunde A Ayodele, Neil M O'Brien-Simpson, Walter Birchmeier, Robert N Pike, Charles N Pagel, Eleanor J Mackie

Journal article | 2018 | Cellular Microbiology

Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice

Vladimir Ilievski, Paulina K Zuchowska, Stefan J Green, Peter T Toth, Michael E Ragozzino, Khuong Le, Haider W Aljewari, Neil M O'Brien-Simpson, Eric C Reynolds, Keiko Watanabe

Journal article | 2018 | PLOS ONE

Designing antibiotic peptide polymer adjuvants for multidrug resistant bacteria

Neil O'Brien-Simpson, Shu Lam, Jason C Lenzo, James Holden, Greg Qiao, Eric Reynolds

Conference Proceedings | 2018 | JOURNAL OF PEPTIDE SCIENCE

Interplay between Porphyromonas gingivalis and EGF signalling in the regulation of CXCL14

Jiamin Aw, Glen M Scholz, Noorjahan Laila Huq, Jennifer Huynh, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2018 | Cellular Microbiology

Covalent conjugation of cationic antimicrobial peptides with a beta-lactam antibiotic core

Wenyi Li, Neil M O'Brien-Simpson, James A Holden, Laszlo Otvos, Eric C Reynolds, Frances Separovic, Mohammed Akhter Hossain, John D Wade

Journal article | 2018 | PEPTIDE SCIENCE

Comparative study of novel in situ decorated porous chitosan-selenium scaffolds and porous chitosan-silver scaffolds towards antimicrobial wound dressing application

Dhee P Biswas, Neil M O'Brien-Simpson, Eric C Reynolds, Andrea J O'Connor, Phong A Tran

Journal article | 2018 | Journal of Colloid and Interface Science

Antimicrobial activity of simplified mimics of celogentin C

Varsha J Thombare, James A Holden, Sreetama Pal, Eric C Reynolds, Amitabha Chattopadhyay, Neil M O'Brien-Simpson, Craig A Hutton

Journal article | 2018 | Tetrahedron

Biocompatibility and Osteogenic/Calcification Potential of Casein Phosphopeptide-amorphous Calcium Phosphate Fluoride

Alaa E Dawood, David J Manton, Peter Parashos, Rebecca H Wong, William Singleton, James A Holden, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2018 | Journal of Endodontics

Editorial: Antimicrobial and Anticancer Peptides

Neil M O'Brien-Simpson, Ralf Hoffmann, CS Brian Chia, John D Wade

Journal article | 2018 | Frontiers in Chemistry

Adolescent temperament dimensions as stable prospective risk and protective factors for salivary C-reactive protein

Benjamin W Nelson, Michelle L Byrne, Julian G Simmons, Sarah Whittle, Orli S Schwartz, Neil M O'Brien-Simpson, Katrina A Walsh, Eric C Reynolds, Nicholas B Allen

Journal article | 2018 | British Journal of Health Psychology

Adolescent Sympathetic Activity and Salivary C-Reactive Protein: The Effects of Parental Behavior

Benjamin W Nelson, Michelle L Byrne, Julian G Simmons, Sarah Whittle, Orli S Schwartz, Eric C Reynolds, Neil M O'Brien-Simpson, Lisa Sheeber, Nicholas B Allen

Journal article | 2017 | Health Psychology

Porphyromonas gulae Activates Unprimed and Gamma Interferon-Primed Macrophages via the Pattern Recognition Receptors Toll-Like Receptor 2 (TLR2), TLR4, and NOD2

James A Holden, Neil M O'Brien-Simpson, Jason C Lenzo, Rebecca KH Orth, Ashley Mansell, Eric C Reynolds

Journal article | 2017 | Infection and Immunity

Outer Membrane Vesicles Prime and Activate Macrophage Inflammasomes and Cytokine Secretion In Vitro and In Vivo

Jessica D Cecil, Neil M O'Brien-Simpson, Jason C Lenzo, James A Holden, William Singleton, Alexis Perez-Gonzalez, Ashley Mansell, Eric C Reynolds

Journal article | 2017 | Frontiers in Immunology

Metabolic Remodeling, Inflammasome Activation, and Pyroptosis in Macrophages Stimulated by Porphyromonas gingivalis and Its Outer Membrane Vesicles

Andrew J Fleetwood, Man KS Lee, William Singleton, Adrian Achuthan, Ming-Chin Lee, Neil M O'Brien-Simpson, Andrew D Cook, Andrew J Murphy, Stuart G Dashper, Eric C Reynolds, John A Hamilton

Journal article | 2017 | Frontiers in Cellular and Infection Microbiology

Associations Between Observed Parenting Behavior and Adolescent Inflammation Two and a Half Years Later in a Community Sample

Michelle L Byrne, Sally Horne, Neil M O'Brien-Simpson, Katrina A Walsh, Eric C Reynolds, Orli S Schwartz, Sarah Whittle, Julian G Simmons, Lisa Sheeber, Nicholas B Allen

Journal article | 2017 | Health Psychology

Rapid Chair-Side Test for Detection of Porphyromonas gingivalis

NM O'Brien-Simpson, K Burgess, JC Lenzo, GC Brammar, IB Darby, EC Reynolds

Journal article | 2017 | Journal of Dental Research

The Effect of Selective D- or N-alpha-Methyl Arginine Substitution on the Activity of the Proline-Rich Antimicrobial Peptide, Chex1-Arg20

Wenyi Li, Zhe Sun, Neil M O'Brien-Simpson, Laszlo Otvos, Eric C Reynolds, Mohammed A Hossain, Frances Separovic, John D Walde

Journal article | 2017 | Frontiers in Chemistry

C-Terminal Modification and Multimerization Increase the Efficacy of a Proline-Rich Antimicrobial Peptide

Wenyi Li, Neil M O'Brien-Simpson, Shenggen Yao, Julien Tailhades, Eric C Reynolds, Raymond M Dawson, Laszlo Otvos, Mohammed Akhter Hossain, Frances Separovic, John D Wade

Journal article | 2017 | Chemistry - A European Journal

Fluorescent Ion Efflux Screening Assay for Determining Membrane-Active Peptides

Neil M O'Brien-Simpson, Wenyi Li, Namfon Pantarat, Mohammed Akhter Hossain, Frances Separovic, John D Wade, Eric C Reynolds

Journal article | 2017 | Australian Journal of Chemistry

Bionano Interaction Study on Antimicrobial Star-Shaped Peptide Polymer Nanoparticles

Shu J Lam, Edgar HH Wong, Neil M O'Brien-Simpson, Namfon Pantarat, Anton Blencowe, Eric C Reynolds, Greg G Qiao

Journal article | 2016 | ACS Applied Materials & Interfaces

A therapeutic Porphyromonas gingivalis gingipain vaccine induces neutralising IgG1 antibodies that protect against experimental periodontitis

Neil M O'Brien-Simpson, James A Holden, Jason C Lenzo, Yan Tan, Gail C Brammar, Katrina A Walsh, William Singleton, Rebecca KH Orth, Nada Slakeski, Keith J Cross, Ivan B Darby, Dorit Becher, Tony Rowe, Adriana Baz Morelli, Andrew Hammet, Andrew Nash, Anna Brown, Bing Ma, Didier Vingadassalom, Jacqueline McCluskey, et al.

Journal article | 2016 | npj Vaccines

Codelivery of NOD2 and TLR9 Ligands via Nanoengineered Protein Antigen Particles for Improving and Tuning Immune Responses

Katelyn T Gause, Yan Yan, Neil M O'Brien-Simpson, Jiwei Cui, Jason C Lenzo, Eric C Reynolds, Frank Caruso

Journal article | 2016 | Advanced Functional Materials

Candida virulence and ethanol-derived acetaldehyde production in oral cancer and non-cancer subjects

AD Alnuaimi, AN Ramdzan, D Wiesenfeld, NM O'Brien-Simpson, SD Kolev, EC Reynolds, MJ McCullough

Journal article | 2016 | Oral Diseases

Combating multidrug-resistant Gram-negative bacteria with structurally nanoengineered antimicrobial peptide polymers

Shu J Lam, Neil M O'Brien-Simpson, Namfon Pantarat, Adrian Sulistio, Edgar HH Wong, Yu-Yen Chen, Jason C Lenzo, James A Holden, Anton Blencowe, Eric C Reynolds, Greg G Qiao

Journal article | 2016 | Nature Microbiology

Porphyromonas gulae Has Virulence and Immunological Characteristics Similar to Those of the Human Periodontal Pathogen Porphyromonas gingivalis

Jason C Lenzo, Neil M O'Brien-Simpson, Rebecca K Orth, Helen L Mitchell, Stuart G Dashper, Eric C Reynolds

Journal article | 2016 | Infection and Immunity

Unprimed, M1 and M2 macrophages differentially interact with Porphyromonas gingivalis

R Lam, N O'Brien-Simpson, J Holden, J Lenzo, SB Fong, E Reynolds

Conference Proceedings | 2016 | EUROPEAN JOURNAL OF IMMUNOLOGY

Porphyromonas gingivalis-derived RgpA-Kgp complex activates the macrophage urokinase plasminogen activator system

A Fleetwood, N O'Brien-Simpson, P Veith, R Lam, A Achuthan, A Cook, W Singleton, I Lund, E Reynolds, J Hamilton

Conference Proceedings | 2016 | EUROPEAN JOURNAL OF IMMUNOLOGY

Porphyromonas gulae activates M1 macrophages via TLR2, TLR4 and NOD2

J Holden, J Lenzo, R Orth, NM O'Brien-Simpson, EC Reynolds

Conference Proceedings | 2016 | EUROPEAN JOURNAL OF IMMUNOLOGY

Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis

Roselind S Lam, Neil M O'Brien-Simpson, James A Holden, Jason C Lenzo, Shao B Fong, Eric C Reynolds

Journal article | 2016 | PLOS ONE

Determination of Active Phagocytosis of Unopsonized Porphyromonas gingivalis by Macrophages and Neutrophils Using the pH-Sensitive Fluorescent Dye pHrodo

Jason C Lenzo, Neil M O'Brien-Simpson, Jessica Cecil, James A Holden, Eric C Reynolds

Journal article | 2016 | Infection and Immunity

Polypeptide-Based Macroporous Cryogels with Inherent Antimicrobial Properties: The Importance of a Macroporous Structure

Steven J Shirbin, Shu J Lam, Nicholas Jun-An Chan, Mehmet Murat Ozmen, Qiang Fu, Neil O'Brien-Simpson, Eric C Reynolds, Greg G Qiao

Journal article | 2016 | ACS Macro Letters

Differential Responses of Pattern Recognition Receptors to Outer Membrane Vesicles of Three Periodontal Pathogens

Jessica D Cecil, Neil M O'Brien-Simpson, Jason C Lenzo, James A Holden, Yu-Yen Chen, William Singleton, Katelyn T Gause, Yan Yan, Frank Caruso, Eric C Reynolds

Journal article | 2016 | PLOS ONE

A Rapid and Quantitative Flow Cytometry Method for the Analysis of Membrane Disruptive Antimicrobial Activity

Neil M O'Brien-Simpson, Namfon Pantarat, Troy J Attard, Katrina A Walsh, Eric C Reynolds

Journal article | 2016 | PLOS ONE

Low cytotoxic trace element selenium nanoparticles and their differential antimicrobial properties against S-aureus and E-coli

Phong A Tran, Neil O'Brien-Simpson, Eric C Reynolds, Namfon Pantarat, Dhee P Biswas, Andrea J O'Connor

Journal article | 2016 | Nanotechnology

Meet our editorial board member

N O’Brien-Simpson

Journal article | 2016 | Current Protein & Peptide Science

Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice

LM Judd, RG Heine, TR Menheniott, J Buzzelli, N O'Brien-Simpson, D Pavlic, L O'Connor, K Al Gazali, O Hamilton, M Scurr, AM Collison, J Mattes, KJ Allen, AS Giraud

Journal article | 2016 | American Journal of Physiology-Gastrointestinal and Liver Physiology

Tannerella forsythia Outer Membrane Vesicles Are Enriched with Substrates of the Type IX Secretion System and TonB-Dependent Receptors

Paul D Veith, Yu-Yen Chen, Dina Chen, Neil M O'Brien-Simpson, Jessica D Cecil, James A Holden, Jason C Lenzo, Eric C Reynolds

Journal article | 2015 | Journal of Proteome Research

Adolescent-Onset Depression: Are Obesity and Inflammation Developmental Mechanisms or Outcomes?

Michelle L Byrne, Neil M O'Brien-Simpson, Sarah A Mitchell, Nicholas B Allen

Journal article | 2015 | Child Psychiatry & Human Development

The SENSE Study (Sleep and Education: learning New Skills Early): a community cognitive-behavioural therapy and mindfulness-based sleep intervention to prevent depression and improve cardiac health in adolescence.

Joanna M Waloszek, Orli Schwartz, Julian G Simmons, Matthew Blake, Laura Blake, Greg Murray, Monika Raniti, Ronald E Dahl, Neil O'Brien-Simpson, Paul Dudgeon, John Trinder, Nicholas B Allen

Journal article | 2015 | BMC Psychology

BACKGROUND: Sleep problems are a major risk factor for the emergence of depression in adolescence. The aim of this study was to te..

Multimerization of a Proline-Rich Antimicrobial Peptide, Chex-Arg20, Alters Its Mechanism of Interaction with the Escherichia coli Membrane

Wenyi Li, Neil M O'Brien-Simpson, Julien Tailhades, Namfon Pantarat, Raymond M Dawson, Laszlo Otvos, Eric C Reynolds, Frances Separovic, Mohammed Akhter Hossain, John D Wade

Journal article | 2015 | Chemistry & Biology

GM-CSF and uPA are required for Porphyromonas gingivalis-induced alveolar bone loss in a mouse periodontitis model

Roselind S Lam, Neil M O'Brien-Simpson, John A Hamilton, Jason C Lenzo, James A Holden, Gail C Brammar, Rebecca K Orth, Yan Tan, Katrina A Walsh, Andrew J Fleetwood, Eric C Reynolds

Journal article | 2015 | Immunology & Cell Biology

Nanostructured Selenium Coating for Anti-infective Medical Implants: in vitro and in vivo pilot studies

PA Tran, N O'Brien-Simpson, EC Reynolds, K Abberton, J Palmer, W Morrison, A O'Connor

Conference Proceedings | 2015 | TISSUE ENGINEERING PART A

Antimicrobial peptide stars: The road to discovery and development

Shu Jie Lam, Neil O'Brien-Simpson, Namfon Pantarat, Adrian Sulistio, Edgar Hoe Hon Wong, Anton Blencowe, Eric Reynolds, Greg Qiao

Conference Proceedings | 2015 | ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY

SNAPPing Gram-negative bacteria with star-shaped polypeptides

Shu Jie Lam, Neil O'Brien-Simpson, Namfon Pantarat, Adrian Sulistio, Edgar Hoe Hon Wong, Yu-Yen Chen, Anton Blencowe, Eric Reynolds, Greg Qiao

Conference Proceedings | 2015 | ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY

Porphyromonas gingivalis-derived RgpA-Kgp Complex Activates the Macrophage Urokinase Plasminogen Activator System IMPLICATIONS FOR PERIODONTITIS

Andrew J Fleetwood, Neil M O'Brien-Simpson, Paul D Veith, Roselind S Lam, Adrian Achuthan, Andrew D Cook, William Singleton, Ida K Lund, Eric C Reynolds, John A Hamilton

Journal article | 2015 | Journal of Biological Chemistry

Physicochemical and Immunological Assessment of Engineered Pure Protein Particles with Different Redox States

Katelyn T Gause, Yan Yan, Jiwei Cui, Neil M O'Brien-Simpson, Jason C Lenzo, Eric C Reynolds, Frank Caruso

Journal article | 2015 | ACS Nano

Oral Candida colonization in oral cancer patients and its relationship with traditional risk factors of oral cancer: A matched case-control study

Ali D Alnuaimi, David Wiesenfeld, Neil M O'Brien-Simpson, Eric C Reynolds, Michael J McCullough

Journal article | 2015 | Oral Oncology

Prospects for treatment of Porphyromonas gingivalis-mediated disease - immune-based therapy

Eric C Reynolds, Neil O'Brien-Simpson, Tony Rowe, Andrew Nash, Jackie McCluskey, Didier Vingadassalom, Harold Kleanthous

Journal article | 2015 | Journal of oral microbiology

C-Terminal Modifications Broaden Activity of the Proline-Rich Antimicrobial Peptide, Chex1-Arg20

Wenyi Li, Julien Tailhades, M Akhter Hossain, Neil M O'Brien-Simpson, Eric C Reynolds, Laszlo Otvos, Frances Separovic, John D Wade

Journal article | 2015 | Australian Journal of Chemistry

Prospects for treatment of Porphyromonas gingivalis-mediated disease – immune-based therapy

Eric C Reynolds, Neil O'Brien-Simpson, Tony Rowe, Andrew Nash, Jackie McCluskey, Didier Vingadassalom, Harold Kleanthous

Journal article | 2015 | Journal of Oral Microbiology

Development and evaluation of a saliva-based chair-side diagnostic for the detection of Porphyromonas gingivalis

Neil M O'Brien-Simpson, Kate Burgess, Gail C Brammar, Ivan B Darby, Eric C Reynolds

Journal article | 2015 | Journal of Oral Microbiology

Antibiofouling polymer interfaces: poly(ethylene glycol) and other promising candidates

Sean Lowe, Neil M O'Brien-Simpson, Luke A Connal

Journal article | 2015 | Polymer Chemistry

Porphyromonas gingivalis Lipopolysaccharide Weakly Activates M1 and M2 Polarized Mouse Macrophages but Induces Inflammatory Cytokines

James A Holden, Troy J Attard, Katrina M Laughton, Ashley Mansell, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2014 | Infection and Immunity

Fabrication of planarised conductively patterned diamond for bio-applications

Wei Tong, Kate Fox, Kumaravelu Ganesan, Ann M Turnley, Olga Shimoni, Phong A Tran, Alexander Lohrmann, Thomas McFarlane, Arman Ahnood, David J Garrett, Hamish Meffin, Neil M O'Brien-Simpson, Eric C Reynolds, Steven Prawer

Journal article | 2014 | Materials Science and Engineering: C

Proline-rich antimicrobial peptides: potential therapeutics against antibiotic-resistant bacteria

Wenyi Li, Julien Tailhades, Neil M O'Brien-Simpson, Frances Separovic, Laszlo Otvos, M Akhter Hossain, John D Wade

Journal article | 2014 | Amino Acids

Macrophage Depletion Abates Porphyromonas gingivalis-Induced Alveolar Bone Resorption in Mice

Roselind S Lam, Neil M O'Brien-Simpson, Jason C Lenzo, James A Holden, Gail C Brammar, Katrina A Walsh, Judith E McNaughtan, Dennis K Rowler, Nico Van Rooijen, Eric C Reynolds

Journal article | 2014 | Journal of Immunology

The development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis

AD Alnuaimi, D Wiesenfeld, NM O'Brien-Simpson, EC Reynolds, B Peng, MJ McCullough

Journal article | 2014 | Molecular Oral Microbiology

Porphyromonas gingivalis Outer Membrane Vesicles Exclusively Contain Outer Membrane and Periplasmic Proteins and Carry a Cargo Enriched with Virulence Factors

Paul D Veith, Yu-Yen Chen, Dhana G Gorasia, Dina Chen, Michelle D Glew, Neil M O'Brien-Simpson, Jessica D Cecil, James A Holden, Eric C Reynolds

Journal article | 2014 | Journal of Proteome Research

Dye release experiments with dextran loaded vesicles

M Sani, NM O'Brien-Simpson, F Separovic

Journal article | 2014 | Bio-protocol

Oxantel Disrupts Polymicrobial Biofilm Development of Periodontal Pathogens

Stuart Dashper, Neil O'Brien-Simpson, Sze Wei Liu, Rita Paolini, Helen Mitchell, Katrina Walsh, Tanya D'Cruze, Brigitte Hoffmann, Deanne Catmull, Ying Zhu, Eric Reynolds

Journal article | 2014 | Antimicrobial Agents and Chemotherapy

Bacterial fluorescent-dextran diffusion assay

NM O'Brien-Simpson, N Pantarat, KA Walsh, EC Reynolds, M Sani, F Separovic

Journal article | 2014 | Bio-protocol

Differential Roles of the Protein Corona in the Cellular Uptake of Nanoporous Polymer Particles by Monocyte and Macrophage Cell Lines

Yan Yan, Katelyn T Gause, Marloes MJ Kamphuis, Ching-Seng Ang, Neil M O'Brien-Simpson, Jason C Lenzo, Eric C Reynolds, Edouard C Nice, Frank Caruso

Journal article | 2013 | ACS Nano

Clinical isolates and laboratory reference Candida species and strains have varying abilities to form biofilms

Ali D Alnuaimi, Neil M O'Brien-Simpson, Eric C Reynolds, Michael J McCullough

Journal article | 2013 | FEMS Yeast Research

Acute phase protein and cytokine levels in serum and saliva: A comparison of detectable levels and correlations in a depressed and healthy adolescent sample

Michelle L Byrne, Neil M O'Brien-Simpson, Eric C Reynolds, Katrina A Walsh, Katrina Laughton, Joanna M Waloszek, Michael J Woods, John Trinder, Nicholas B Allen

Journal article | 2013 | Brain, Behavior and Immunity

Oral Health Risk Factors for Bisphosphonate-Associated Jaw Osteonecrosis

Claudine Tsao, Ivan Darby, Peter R Ebeling, Katrina Walsh, Neil O'Brien-Simpson, Eric Reynolds, Gelsomina Borromeo

Journal article | 2013 | Journal of Oral and Maxillofacial Surgery

Maculatin 1.1 Disrupts Staphylococcus aureus Lipid Membranes via a Pore Mechanism

M-A Sani, TC Whitwell, JD Gehman, RM Robins-Browne, N Pantarat, TJ Attard, EC Reynolds, NM O'Brien-Simpson, F Separovic

Journal article | 2013 | Antimicrobial Agents and Chemotherapy

Streptococcus mutans biofilm disruption by kappa-casein glycopeptide

Stuart G Dashper, Sze-Wei Liu, Katrina A Walsh, Geoffrey G Adams, David P Stanton, Brent R Ward, Peiyan Shen, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2013 | Journal of Dentistry

Editorial for the Special Issue for the 3rd Modern Solid Phase Peptide Synthesis and its Applications Symposium

Neil M O'Brien-Simpson, John D Wade

Journal article | 2013 | International Journal of Peptide Research and Therapeutics

Polymerisation of a T Cell Epitope with an Immunostimulatory C3d Peptide Sequence Enhances Antigen Specific T Cell Responses

Neil M O'Brien-Simpson, Troy J Attard, Baihui Zheng, Katrina A Walsh, Eric C Reynolds

Journal article | 2013 | International Journal of Peptide Research and Therapeutics

DEPRESSIVE SYMPTOMS AND C-REACTIVE PROTEIN IN ADOLESCENTS: EFFECTS OF BMI AND SEX

Michelle L Byrne, Neil O'Brien-Simpson, Nicholas B Allen

Conference Proceedings | 2012 | PSYCHOPHYSIOLOGY

Porphyromonas gingivalis LPS induces cytokine secretion in macrophages without costimulatory marker expression

J Holden, NM O'Brien-Simpson, EC Reynolds

Journal article | 2012 | Immunology

The role of gamma delta T cells in Porphyromonas gingivalis-induced periodontal disease

J Lenzo, NM O'Brien-Simpson, V Tam, EC Reynolds

Conference Proceedings | 2012 | IMMUNOLOGY

Synergistic virulence of Porphyromonas gingivalis and Treponema denticola in a murine periodontitis model

RK-H Orth, NM O'Brien-Simpson, SG Dashper, EC Reynolds

Journal article | 2011 | Molecular Oral Microbiology

The outer membrane protein LptO is essential for the O-deacylation of LPS and the co-ordinated secretion and attachment of A-LPS and CTD proteins in Porphyromonas gingivalis

Yu-Yen Chen, Benjamin Peng, Qiaohui Yang, Michelle D Glew, Paul D Veith, Keith J Cross, Kenneth N Goldie, Dina Chen, Neil O'Brien-Simpson, Stuart G Dashper, Eric C Reynolds

Journal article | 2011 | Molecular Microbiology

Porphyromonas gingivalis Cysteine Proteinase Inhibition by kappa-Casein Peptides

Elena CY Toh, Stuart G Dashper, N Laila Huq, Troy J Attard, Neil M O'Brien-Simpson, Yu-Yen Chen, Keith J Cross, David P Stanton, Rita A Paolini, Eric C Reynolds

Journal article | 2011 | Antimicrobial Agents and Chemotherapy

Editorial for the Special Issue on the 2nd Modern Solid Phase Peptide Synthesis and its Applications Symposium

Neil M O'Brien-Simpson, John D Wade

Journal article | 2010 | International Journal of Peptide Research and Therapeutics

An efficient method for enumerating oral spirochetes using flow cytometry

Rebecca Orth, Neil O'Brien-Simpson, Stuart Dashper, Katrina Walsh, Eric Reynolds

Journal article | 2010 | Journal of Microbiological Methods

Protease-Activated Receptor 2 Has Pivotal Roles in Cellular Mechanisms Involved in Experimental Periodontitis

David M Wong, Vivian Tam, Roselind Lam, Katrina A Walsh, Liliana Tatarczuch, Charles N Pagel, Eric C Reynolds, Neil M O'Brien-Simpson, Eleanor J Mackie, Robert N Pike

Journal article | 2010 | Infection and Immunity

Host immune responses to Porphyromonas gingivalis antigens

Rishi D Pathirana, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2010 | Periodontology 2000

Major proteins and antigens of Treponema denticola

Paul D Veith, Stuart G Dashper, Neil M O'Brien-Simpson, Rita A Paolini, Rebecca Orth, Katrina A Walsh, Eric C Reynolds

Journal article | 2009 | Biochimica et Biophysica Acta-Proteins and Proteomics

Outer Membrane Proteome and Antigens of Tannerella forsythia

Paul D Veith, Neil M O'Brien-Simpson, Yan Tan, Deasy C Djatmiko, Stuart G Dashper, Eric C Reynolds

Journal article | 2009 | Journal of Proteome Research

The A-chain of insulin is a hot-spot for CD4( ) T cell epitopes in human type 1 diabetes

SI Mannering, SH Pang, NA Williamson, G Naselli, EC Reynolds, NM O'Brien-Simpson, AW Purcell, LC Harrison

Journal article | 2009 | Clinical and Experimental Immunology

The RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Inactivate the Th2 Cytokines Interleukin-4 and Interleukin-5

Vivian Tam, Neil M O'Brien-Simpson, Yu-Yen Chen, Colin J Sanderson, Beverley Kinnear, Eric C Reynolds

Journal article | 2009 | Infection and Immunity

Porphyromonas gingivalis RgpA-Kgp Proteinase-Adhesin Complexes Penetrate Gingival Tissue and Induce Proinflammatory Cytokines or Apoptosis in a Concentration-Dependent Manner

Neil M O'Brien-Simpson, Rishi D Pathirana, Glenn D Walker, Eric C Reynolds

Journal article | 2009 | Infection and Immunity

Identification and Suppression of beta-Elimination Byproducts Arising from the Use of Fmoc-Ser(PO(3)Bzl,H)-OH in Peptide Synthesis

Troy J Attard, Neil M O'Brien-Simpson, Eric C Reynolds

Journal article | 2009 | International Journal of Peptide Research and Therapeutics

Response of Porphyromonas gingivalis to Heme Limitation in Continuous Culture

Stuart G Dashper, Ching-Seng Ang, Paul D Veith, Helen L Mitchell, Alvin WH Lo, Christine A Seers, Katrina A Walsh, Nada Slakeski, Dina Chen, J Patricia Lissel, Catherine A Butler, Neil M O'Brien-Simpson, Ian G Barr, Eric C Reynolds

Journal article | 2009 | Journal of Bacteriology

Modern Solid Phase Peptide Synthesis and its Applications

Neil M O'Brien-Simpson, Nicholas J Ede, John D Wade

Journal article | 2008 | International Journal of Peptide Research and Therapeutics

The role of the RgpA-Kgp proteinase-adhesin complexes in the adherence of Porphyromonas gingivalis to fibroblasts

Rishi D Pathirana, Neil M O'Brien-Simpson, Kumar Visvanathan, John A Hamilton, Eric C Reynolds

Journal article | 2008 | Microbiology

Characterization of T cell responses to the RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis in BALB/c mice

Vivian Tam, Neil M O'Brien-Simpson, Rishi D Pathirana, Leanne T Frazer, Eric C Reynolds

Journal article | 2008 | Journal of Immunology

ChemInform Abstract: Synthesis of Phosphopeptides in the Fmoc Mode

Troy J Attard, Neil O'Brien-Simpson, Eric C Reynolds

Journal article | 2008 | ChemInform

Synthesis and characterisation of a multiphosphorylated phosphophoryn repeat motif; H-[Asp-(Ser(P))(2)](3)-Asp-OH

Neil M O'Brien-Simpson, Troy J Attard, Ashwini Loganathan, N Laila Huq, Keith J Cross, Peter F Riley, Eric C Reynolds

Journal article | 2007 | International Journal of Peptide Research and Therapeutics

The role of multiphosphorylated peptides in mineralized tissue regeneration

Keith J Cross, N Laila Huq, Neil M O'Brien-Simpson, John W Perich, Troy J Attard, Eric C Reynolds

Journal article | 2007 | International Journal of Peptide Research and Therapeutics

Synthesis of phosphopeptides in the fmoc mode

Troy J Attard, Neil O'Brien-Simpson, Eric C Reynolds

Journal article | 2007 | International Journal of Peptide Research and Therapeutics

The Role of Multiphosphorylated Peptides in Mineralized Tissue Regeneration

Keith J Cross, N Laila Huq, Neil M O’Brien-Simpson, John W Perich, Troy J Attard, Eric C Reynolds

Journal article | 2007 | International Journal of Peptide Research and Therapeutics

Flow cytometric analysis of adherence of Porphyromonas gingivalis to oral epithelial cells

Rishi D Pathirana, Neil M O'Brien-Simpson, Kumar Visvanathan, John A Hamilton, Eric C Reynolds

Journal article | 2007 | Infection and Immunity

Kgp and RgpB, but not RgpA, are important for Porphyromonas gingivalis virulence in the murine periodontitis model

Rishi D Pathirana, Neil M O'Brien-Simpson, Gail C Brammar, Nada Slakeski, Eric C Reynolds

Journal article | 2007 | Infection and Immunity

Synthetic peptide constructs for the diagnosis and treatment of periodontitis associated with Porphyromonas gingivalis

E REYNOLDS, N O'BRIEN-SIMPSON, N SLAKESKI

Patent | 2007

Vaccination with recombinant adhesins from the RgpA-Kgp proteinase-adhesin complex protects against Porphyromonas gingivalis infection

Leanne T Frazer, Neil M O'Brien-Simpson, Nada Slakeski, Katrina A Walsh, Paul D Veith, Chao Guang Chen, Ian G Barr, Eric C Reynolds

Journal article | 2006 | Vaccine

Characterization of proteinase-adhesin complexes of Porphyromonas gingivalis

Rishi D Pathirana, Neil M O'Brien-Simpson, Paul D Veith, Peter F Riley, Eric C Reynolds

Journal article | 2006 | Microbiology

An immune response directed to proteinase and adhesin functional epitopes protects against Porphyromonas gingivalis-induced periodontal bone loss

NM O'Brien-Simpson, RD Pathirana, RA Paolini, YY Chen, PD Veith, V Tam, N Ally, RN Pike, EC Reynolds

Journal article | 2005 | Journal of Immunology

A novel Porphyromonas gingivalis FeoB plays a role in manganese accumulation

SG Dashper, CA Butler, JP Lissel, RA Paolini, B Hoffmann, PD Veith, NM O'Brien-Simpson, SL Snelgrove, JT Tsiros, EC Reynolds

Journal article | 2005 | Journal of Biological Chemistry

Divalent metal cations increase the activity of the antimicrobial peptide kappacin

SG Dashper, NM O'Brien-Simpson, KJ Cross, RA Paolini, B Hoffmann, DV Catmull, M Malkoski, EC Reynolds

Journal article | 2005 | Antimicrobial Agents and Chemotherapy

Synthetic peptides containing protective epitopes for the treatment and prevention of periodontitis associated with porphyromonas gingivalis

N O'BRIEN-SIMPSON, E REYNOLDS

Patent | 2005

Antigens of bacteria associated with periodontitis

NM O'Brien-Simpson, PD Veith, SG Dashper, EC Reynolds

Journal article | 2004 | Periodontology 2000