Zhiguang Xiao

Principles and practice of determining metal-protein affinities.

Tessa R Young, Zhiguang Xiao

Journal article  |  2021  |  Biochem J

Metal ions play many critical roles in biology, as structural and catalytic cofactors, and as cell regulatory and signalling eleme..

9 COPING WITH TOXIC METALS

Book Chapter  |  2021

di-Cysteine Residues of the Arabidopsis thaliana HMA4 C-Terminus Are Only Partially Required for Cadmium Transport

Stanislaus Antony Ceasar, Gilles Lekeux, Patrick Motte, Zhiguang Xiao, Moreno Galleni, Marc Hanikenne

Journal article  |  2020  |  FRONTIERS IN PLANT SCIENCE

Cadmium (Cd) is highly toxic to the environment and humans. Plants are capable of absorbing Cd from the soil and of transporting p..

The Scs disulfide reductase system cooperates with the metallochaperone CueP in Salmonella copper resistance

Pramod Subedi, Jason J Paxman, Geqing Wang, Ashwinie A Ukuwela, Zhiguang Xiao, Begona Heras

Journal article  |  2019  |  JOURNAL OF BIOLOGICAL CHEMISTRY

The human pathogen Salmonella enterica serovar Typhimurium (S Typhimurium) contains a complex disulfide bond (Dsb) catalytic machi..

The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family

Saumya R Udagedara, Chathuri JK Wijekoon, Zhiguang Xiao, Anthony G Wedd, Megan J Maher

Journal article  |  2019  |  JOURNAL OF INORGANIC BIOCHEMISTRY

The bacterial CopC family of proteins are periplasmic copper binding proteins that act in copper detoxification. These proteins co..

Molecular Mechanisms of Glutaredoxin Enzymes: Versatile Hubs for Thiol-Disulfide Exchange between Protein Thiols and Glutathione

Zhiguang Xiao, Sharon La Fontaine, Ashley I Bush, Anthony G Wedd

Journal article  |  2019  |  JOURNAL OF MOLECULAR BIOLOGY

The tripeptide glutathione (GSH) and its oxidized form glutathione disulfide (GSSG) constitute a key redox couple in cells. In par..

di-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo

Gilles Lekeux, Clementine Laurent, Marine Joris, Alice Jadoul, Dan Jiang, Bernard Bosman, Monique Carnol, Patrick Motte, Zhiguang Xiao, Moreno Galleni, Marc Hanikenne

Journal article  |  2018  |  Journal of Experimental Botany

The PIB ATPase heavy metal ATPase 4 (HMA4) has a central role in the zinc homeostasis network of Arabidopsis thaliana. This membra..

The Human Amyloid Precursor Protein Binds Copper Ions Dominated by a Picomolar-Affinity Site in the Helix-Rich E2 Domain

Tessa R Young, Tara L Pukala, Roberto Cappai, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  BIOCHEMISTRY

A manifestation of Alzheimer's disease (AD) is the aggregation in the brain of amyloid β (Aβ) peptides derived from the amyloid pr..

Glutaredoxins employ parallel monothiol-dithiol mechanisms to catalyze thiol-disulfide exchanges with protein disulfides

Ashwinie A Ukuwela, Ashley I Bush, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  CHEMICAL SCIENCE

Glutaredoxins (Grxs) are a family of glutathione (GSH)-dependent thiol-disulfide oxidoreductases. They feature GSH-binding sites t..

Evaluation of Cu(I) binding to the E2 domain of the amyloid precursor protein - a lesson in quantification of metal binding to proteins via ligand competition

Tessa R Young, Anthony G Wedd, Zhiguang Xiao

Journal article  |  2018  |  METALLOMICS

The extracellular domain E2 of the amyloid precursor protein (APP) features a His-rich metal-binding site (denoted as the M1 site)..