Geoff Howlett

Lipid-apolipoprotein interactions in amyloid fibril formation and relevance to atherosclerosis

Geoffrey J Howlett, Timothy M Ryan, Michael DW Griffin

Journal article  |  2019  |  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Polymorphism in disease-related apolipoprotein C-II amyloid fibrils: a structural model for rod-like fibrils

Courtney O Zlatic, Yu Mao, Nevena Todorova, Yee-Foong Mok, Geoffrey J Howlett, Irene Yarovsky, Paul R Gooley, Michael DW Griffin

Journal article  |  2018  |  The FEBS Journal

Intra- and Intersubunit Ion-Pair Interactions Determine the Ability of Apolipoprotein C-II Mutants To Form Hybrid Amyloid Fibrils.

Nevena Todorova, Courtney O Zlatic, Yu Mao, Irene Yarovsky, Geoffrey J Howlett, Paul R Gooley, Michael DW Griffin

Journal article  |  2017  |  Biochemistry

The apolipoprotein family is structurally defined by amphipathic α-helical regions that interact with lipid surfaces. In the absen..

Intra- and Intersubunit Ion-Pair Interactions Determine the Ability of Apolipoprotein C-II Mutants To Form Hybrid Amyloid Fibrils

Nevena Todorova, Courtney O Zlatic, Yu Mao, Irene Yarovsky, Geoffrey J Howlett, Paul R Gooley, Michael DW Griffin

Journal article  |  2017  |  BIOCHEMISTRY

Solution Conditions Affect the Ability of the K30D Mutation To Prevent Amyloid Fibril Formation by Apolipoprotein C-II: Insights from Experiments and Theoretical Simulations

Yu Mao, Nevena Todorova, Courtney O Zlatic, Paul R Gooley, Michael DW Griffin, Geoffrey J Howlett, Irene Yarovsky

Journal article  |  2016  |  Biochemistry

Hydrogen/Deuterium Exchange and Molecular Dynamics Analysis of Amyloid Fibrils Formed by a D69K Charge-Pair Mutant of Human Apolipoprotein C-II

Yu Mao, Courtney O Zlatic, Michael DW Griffin, Geoffrey J Howlett, Nevena Todorova, Irene Yarovsky, Paul R Gooley

Journal article  |  2015  |  Biochemistry

Chameleon 'aggregation-prone' segments of apoA-I: A model of amyloid fibrils formed in apoA-I amyloidosis

Nikolaos N Louros, Paraskevi L Tsiolaki, Michael DW Griffin, Geoffrey J Howlett, Stavros J Hamodrakas, Vassiliki A Iconomidou

Journal article  |  2015  |  International Journal of Biological Macromolecules

Fluphenazine center dot HCl and Epigallocatechin Gal late Modulate the Rate of Formation and Structural Properties of Apolipoprotein C-II Amyloid Fibrils

Courtney O Zlatic, Yu Mao, Timothy M Ryan, Yee-Foong Mok, Blaine R Roberts, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2015  |  Biochemistry

Charge and Charge-Pair Mutations Alter the Rate of Assembly and Structural Properties of Apolipoprotein C-II Amyloid Fibrils

Yu Mao, Chai Lean Teoh, Shuo Yang, Courtney O Zlatic, Zachary K Rosenes, Paul R Gooley, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2015  |  Biochemistry

The Role of Lipid in Misfolding and Amyloid Fibril Formation by Apolipoprotein C-II

Timothy M Ryan, Yee-Foong Mok, Geoffrey J Howlett, Michael DW Griffin

Book Chapter  |  2015  |  Advances in Experimental Medicine and Biology

Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils

Yee-Foong Mok, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2015  |  Methods in Enzymology

Simultaneous Binding of the Anti-Cancer IgM Monoclonal Antibody PAT-SM6 to Low Density Lipoproteins and GRP78

Zachary Rosenes, Yee-Foong Mok, Shuo Yang, Michael DW Griffin, Terrence D Mulhern, Danny M Hatters, Frank Hensel, Geoffrey J Howlett

Journal article  |  2013  |  PLoS One

Avoiding the oligomeric state: alpha B-crystallin inhibits fragmentation and induces dissociation of apolipoprotein C-II amyloid fibrils

Katrina J Binger, Heath Ecroyd, Shuo Yang, John A Carver, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2013  |  FASEB Journal

PcoE - A metal sponge expressed to the periplasm of copper resistance Escherichia coli. Implication of its function role in copper resistance

Matthias Zimmermann, Saumya R Udagedara, Chak Ming Sze, Timothy M Ryan, Geoffrey J Howlett, Zhiguang Xiao, Anthony G Wedd

Journal article  |  2012  |  Journal of Inorganic Biochemistry

The Anti-Cancer IgM Monoclonal Antibody PAT-SM6 Binds with High Avidity to the Unfolded Protein Response Regulator GRP78

Zachary Rosenes, Terrence D Mulhern, Danny M Hatters, Leodevico L Ilag, Barbara E Power, Chris Hosking, Frank Hensel, Geoffrey J Howlett, Yee-Foong Mok

Journal article  |  2012  |  PLoS One

An Equilibrium Model for Linear and Closed-Loop Amyloid Fibril Formation

Shuo Yang, Michael DW Griffin, Katrina J Binger, Peter Schuck, Geoffrey J Howlett

Journal article  |  2012  |  Journal of Molecular Biology

Identification of an amyloid fibril forming peptide comprising residues 46-59 of apolipoprotein A-I

Yuan Qi Wong, Katrina J Binger, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2012  |  FEBS Letters

Small Amphipathic Molecules Modulate Secondary Structure and Amyloid Fibril-forming Kinetics of Alzheimer Disease Peptide A beta(1-42)

Timothy M Ryan, Anna Friedhuber, Monica Lind, Geoffrey J Howlett, Colin Masters, Blaine R Roberts

Journal article  |  2012  |  Journal of Biological Chemistry

A Cyclic Peptide Inhibitor of ApoC-II Peptide Fibril Formation: Mechanistic Insight from NMR and Molecular Dynamics Analysis

Michael DW Griffin, Levi Yeung, Andrew Hung, Nevena Todorova, Yee-Foong Mok, John A Karas, Paul R Gooley, Irene Yarovsky, Geoffrey J Howlett

Journal article  |  2012  |  Journal of Molecular Biology

Small Amphipathic Molecules Modulate Secondary Structure and Amyloid Fibril-forming Kinetics of Alzheimer Disease Peptide A beta(1-42)

TM Ryan, AM Friedhuber, M Lind, GJ Howlett, C Masters, BR Roberts

Journal article  |  2012  |  Journal of Biological Chemistry

Bovine serum albumin unfolds in Couette flow

Innocent B Bekard, Peter Asimakis, Chai L Teoh, Tim Ryan, Geoffrey J Howlett, Joseph Bertolini, Dave E Dunstan

Journal article  |  2012  |  Soft Matter

NBD-Labeled Phospholipid Accelerates Apolipoprotein C-II Amyloid Fibril Formation but Is Not Incorporated into Mature Fibrils

Timothy M Ryan, Michael DW Griffin, Michael F Bailey, Peter Schuck, Geoffrey J Howlett

Journal article  |  2011  |  Biochemistry

Shear Flow Induced Changes in Apolipoprotein C-II Conformation and Amyloid Fibril Formation

Chai Lean Teoh, Innocent B Bekard, Peter Asimakis, Michael DW Griffin, Timothy M Ryan, Dave E Dunstan, Geoffrey J Howlett

Journal article  |  2011  |  Biochemistry

Sedimentation velocity analysis of amyloid oligomers and fibrils using fluorescence detection

Yee-Foong Mok, Timothy M Ryan, Shuo Yang, Danny M Hatters, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2011  |  Methods

High-Affinity Amphipathic Modulators of Amyloid Fibril Nucleation and Elongation

Timothy M Ryan, Michael DW Griffin, Chai Lean Teoh, Jingkai Ooi, Geoffrey J Howlett

Journal article  |  2011  |  Journal of Molecular Biology

A Structural Model for Apolipoprotein C-II Amyloid Fibrils: Experimental Characterization and Molecular Dynamics Simulations

Chai Lean Teoh, Chi LL Pham, Nevena Todorova, Andrew Hung, Craig N Lincoln, Emma Lees, Yuen Han Lam, Katrina J Binger, Neil H Thomson, Sheena E Radford, Trevor A Smith, Shirley A Mueller, Andreas Engel, Michael DW Griffin, Irene Yarovsky, Paul R Gooley, Geoffrey J Howlett

Journal article  |  2011  |  Journal of Molecular Biology

Apolipoproteins and amyloid fibril formation in atherosclerosis

Chai Lean Teoh, Michael DW Griffin, Geoffrey J Howlett

Journal article  |  2011  |  Protein & Cell

Sedimentation Velocity Analysis of Amyloid Fibrils

Le Lan Pham Chi, Yee-Foong Mok, Geoffrey J Howlett

Book Chapter  |  2011  |  Methods in Molecular Biology

Visualization of polymorphism in apolipoprotein C-II amyloid fibrils

Chai L Teoh, Hisashi Yagi, Michael DW Griffin, Yuji Goto, Geoffrey J Howlett

Journal article  |  2011  |  Journal of Biochemistry

Phospholipids Enhance Nucleation but Not Elongation of Apolipoprotein C-II Amyloid Fibrils

Timothy M Ryan, Chai L Teoh, Michael DW Griffin, Michael F Bailey, Peter Schuck, Geoffrey J Howlett

Journal article  |  2010  |  Journal of Molecular Biology

Methionine-Oxidized Amyloid Fibrils Are Poor Substrates for Human Methionine Sulfoxide Reductases A and B2

Katrina J Binger, Michael DW Griffin, Stefan H Heinemann, Geoffrey J Howlett

Journal article  |  2010  |  Biochemistry

Methionine oxidation induces amyloid fibril formation by full-length apolipoprotein A-I

Yuan Qi Wong, Katrina J Binger, Geoffrey J Howlett, Michael DW Griffin

Journal article  |  2010  |  Proceedings of the National Academy of Sciences of the United States of America

Thioflavin T fluorescence in human serum: Correlations with vascular health and cardiovascular risk factors

Michael DW Griffin, Leanne M Wilson, Yee-Foong Mok, Andrzej S Januszewski, Andrew M Wilson, Connie S Karschimkus, Evange Romas, Allan B Lee, Tim Godfrey, Melinda Wong, Laurence Clemens, Alicia J Jenkins, Geoffrey J Howlett

Journal article  |  2010  |  Clinical Biochemistry

Effects of mutation on the amyloidogenic propensity of apolipoprotein C-II60-70 peptide

Nevena Todorova, Andrew Hung, Simon M Maaser, Michael DW Griffin, John Karas, Geoffrey J Howlett, Irene Yarovsky

Journal article  |  2010  |  Physical Chemistry Chemical Physics

Thioflavin T fluorescence in human serum: Correlations with vascular health and cardiovascular risk factors

M GRIFFIN, L WILSON, Y MOK, A JANUSZEWSKI, A WILSON, C KARSCHIMKUS, E ROMAS, A LEE, undefined Godfrey, undefined Wong, undefined Clemens, A JENKINS, G HOWLETT

Journal article  |  2010  |  Clinical Biochemistry

Effect of Oxidation and Mutation on the Conformational Dynamics and Fibril Assembly of Amyloidogenic Peptides Derived from Apolipoprotein C-II

FS Legge, KJ Binger, MDW Griffin, GJ Howlett, D Scanlon, H Treutlein, I Yarovsky

Journal article  |  2009  |  Journal of Physical Chemistry B

Plasmodium falciparum merozoite surface protein 2 is unstructured and forms amyloid-like fibrils

Christopher G Adda, Vince J Murphy, Margaret Sunde, Lynne J Waddington, Jesse Schloegel, Gert H Talbo, Kleo Vingas, Vivian Kienzle, Rosella Masciantonio, Geoffrey J Howlett, Anthony N Hodder, Michael Foley, Robin F Anders

Journal article  |  2009  |  Molecular and Biochemical Parasitology

Lipids Enhance Apolipoprotein C-II-Derived Amyloidogenic Peptide Oligomerization but Inhibit Fibril Formation

Andrew Hung, Michael DW Griffin, Geoffrey J Howlett, Irene Yarovsky

Journal article  |  2009  |  Journal of Physical Chemistry B

Structure and Function of Human Apolipoprotein

PF James, BW Atcliffe, PR Gooley, T Mulhern, GJ Howlett, RA O'Hair, MAP Perugini

Journal article  |  2009  |  Current Topics in Biochemical Research

Fluorescence Detection of a Lipid-induced Tetrameric Intermediate in Amyloid Fibril Formation by Apolipoprotein C-II

Timothy M Ryan, Geoffrey J Howlett, Michael F Bailey

Journal article  |  2008  |  Journal of Biological Chemistry

Effects of oxidation, pH and lipids on amyloidogenic peptide structure: implications for fibril formation?

Andrew Hung, Michael DW Griffin, Geoffrey J Howlett, Irene Yarovsky

Journal article  |  2008  |  European Biophysics Journal with Biophysics Letters

Methionine oxidation inhibits assembly and promotes disassembly of apolipoprotein C-II amyloid fibrils

Katrina J Binger, Michael DW Griffin, Geoffrey J Howlett

Journal article  |  2008  |  Biochemistry

Experimental determination of quantum dot size distributions, ligand packing densities, and bioconjugation using analytical ultracentrifugation

Emma E Lees, Menachem J Gunzburg, Tich-Lam Nguyen, Geoffrey J Howlett, Julie Rothacker, Edouard C Nice, Andrew HA Clayton, Paul Mulvaney

Journal article  |  2008  |  Nano Letters

Apolipoprotein C-II amyloid fibrils assemble via a reversible pathway that includes fibril breaking and rejoining

Katrina J Binger, Chi LL Pham, Leanne M Wilson, Michael F Bailey, Lynne J Lawrence, Peter Schuck, Geoffrey J Howlett

Journal article  |  2008  |  Journal of Molecular Biology

Phospholipid interaction induces molecular-level polymorphism in apolipoprotein C-II amyloid fibrils via alternative assembly pathways

Michael DW Griffin, Melva LY Mok, Leanne M Wilson, Chi LL Pham, Lynne J Waddington, Matthew A Perugini, Geoffrey J Howlett

Journal article  |  2008  |  Journal of Molecular Biology

 Experimental Determination of Quantum Dot Size Distributions, Ligand Packing Densities, and Bioconjugation Using Analytical Unltracentrifugation.

E LEES, M GUNZBURG, T NGUYEN, G HOWLETT, J Rothacker, E NICE, A CLAYTON, P MULVANEY

Journal article  |  2008  |  Nano Letters

Structural basis for the recognition and cross-linking of amyloid fibrils by human apolipoprotein E

Menachem J Gunzburg, Matthew A Perugini, Geoffrey J Howlett

Journal article  |  2007  |  Journal of Biological Chemistry

Molecular dynamics simulations of a fibrillogenic peptide derived from apolipoprotein C-II

E Sue Legge, Herbert Treutlein, Geoffrey J Howlett, Irene Yarovsky

Journal article  |  2007  |  Biophysical Chemistry

Serum amyloid P colocalizes with apolipoproteins in human atheroma: functional implications

Cameron R Stewart, Antonio Haw, Roland Lopez, Thomas O McDonald, Judy M Callaghan, Malcolm J McConville, Kathryn J Moore, Geoffrey J Howlett, Kevin D O'Brien

Journal article  |  2007  |  Journal of Lipid Research

Oxidized cholesterol metabolites found in human atherosclerotic lesions promote apolipoprotein C-II amyloid fibril formation

Cameron R Stewart, Leanne M Wilson, Qinghai Zhang, Chi LL Pham, Lynne J Waddington, Maree K Staples, David Stapleton, Jeffery W Kelly, Geoffrey J Howlett

Journal article  |  2007  |  Biochemistry

A structural core within apolipoprotein C-II amyloid fibrils identified using hydrogen exchange and proteolysis

Leanne M Wilson, Yee-Foong Mok, Katrina J Binger, Michael DW Griffin, Haydyn DT Mertens, Feng Lin, John D Wade, Paul R Gooley, Geoffrey J Howlett

Journal article  |  2007  |  Journal of Molecular Biology

Serpin acceleration of amyloid fibril formation: A role for accessory proteins

Glenn A Powers, Chi LL Pham, Mary C Pearce, Geoffrey J Howlett, Stephen P Bottomley

Journal article  |  2007  |  Journal of Molecular Biology

Folding of Escherichia coli DsbC: Characterization of a monomeric folding intermediate

Huimin Ke, Sen Zhang, Jian Li, Geoffrey J Howlett, Chih-chen Wang

Journal article  |  2006  |  Biochemistry

Analytical ultracentrifugation for association and assembly the study of protein

Geoffrey J Howlett, Allen P Minton, German Rivas

Journal article  |  2006  |  Current Opinion in Chemical Biology

Untangling the role of amyloid in atherosclerosis

Geoffrey J Howlett, Kathryn J Moore

Journal article  |  2006  |  Current Opinion in Lipidology

High density lipoproteins bind A beta and apolipoprotein C-II amyloid fibrils

LM Wilson, CLL Pham, AJ Jenkins, JD Wade, AF Hill, MA Perugini, GJ Howlett

Journal article  |  2006  |  Journal of Lipid Research

Conformation and orientation of penetratin in phospholipid membranes

AHA Clayton, BW Atcliffe, GJ Howlett, WH Sawyer

Journal article  |  2006  |  Journal of Peptide Science

Shear flow induces amyloid fibril formation

EK Hill, B Krebs, DG Goodall, GJ Howlett, DE Dunstan

Journal article  |  2006  |  Biomacromolecules

Sedimentation velocity analysis of amyloid oligomers and fibrils

Yee-Foong Mok, Geoffrey J Howlett

Journal article  |  2006  |  Methods in Enzymology

The molybdate binding protein Mop from Haemophilus influenzae - Biochemical and thermodynamic characterisation

SL Masters, GJ Howlett, RN Pau

Journal article  |  2005  |  Archives of Biochemistry and Biophysics

Oxidation of low-density lipoproteins induces amyloid-like structures that are recognized by macrophages

CR Stewart, AA Tseng, YF Mok, MK Staples, CH Schiesser, LJ Lawrence, JN Varghese, KJ Moore, GJ Howlett

Journal article  |  2005  |  Biochemistry

The structure and interactions of human apolipoprotein C-II in dodecyl phosphocholine

CA MacRaild, GJ Howlett, PR Gooley

Journal article  |  2004  |  Biochemistry

Non-fibrillar components of amyloid deposits mediate the self-association and tangling of amyloid fibrils

CA MacRaild, CR Stewart, YF Mok, MJ Gunzburg, MA Perugini, LJ Lawrence, V Tirtaatmadja, JJ Cooper-White, GJ Howlett

Journal article  |  2004  |  Journal of Biological Chemistry

C-terminal domain of the membrane copper transporter Ctr1 from Saccharomyces cerevisiae binds four Cu(I) ions as a cuprous-thiolate polynuclear cluster: Sub-femtomolar Cu(I) affinity of three proteins involved in copper trafficking

ZG Xiao, F Loughlin, GN George, GJ Howlett, AG Wedd

Journal article  |  2004  |  Journal of the American Chemical Society

Fibrillar amyloid protein present in atheroma activates CD36 signal transduction

LA Medeiros, T Khan, JB El Khoury, CLL Pham, DM Hatters, GJ Howlett, R Lopez, KD O'Brien, KJ Moore

Journal article  |  2004  |  Journal of Biological Chemistry

Phospholipid complexation and association with apolipoprotein C-II: Insights from mass spectrometry

CL Hanson, LL Ilag, J Malo, DM Hatters, GJ Howlett, CV Robinson

Journal article  |  2003  |  Biophysical Journal

The circularization of amyloid fibrils formed by apolipoprotein C-II

DM Hatters, CA MacRaild, R Daniels, WS Gosal, NH Thomson, JA Jones, JJ Davis, CE MacPhee, CM Dobson, GJ Howlett

Journal article  |  2003  |  Biophysical Journal

Sedimentation velocity analysis of flexible macromolecules: Self-association and tangling of amyloid fibrils

CA MacRaild, DM Hatters, LJ Lawrence, GJ Howlett

Journal article  |  2003  |  Biophysical Journal

Molecular assembly of the ternary granulocyte-macrophage colony-stimulating factor receptor complex

BJ McClure, TR Hercus, BA Cambareri, JM Woodcock, CJ Bagley, GJ Howlett, AF Lopez

Journal article  |  2003  |  Blood

Acid denaturation of alpha(1)-antitrypsin: Characterization of a novel mechanism of serpin polymerization

GL Devlin, MKM Chow, GJ Howlett, SP Bottomley

Journal article  |  2002  |  Journal of Molecular Biology

Cross-linking and amyloid formation by N- and C-terminal cysteine derivatives of human apolipoprotein C-II

CLL Pham, DM Hatters, LJ Lawrence, GJ Howlett

Journal article  |  2002  |  Biochemistry

Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions

MA Perugini, P Schuck, GJ Howlett

Journal article  |  2002  |  European Journal of Biochemistry

The central domain of Escherichia coli TyrR is responsible for hexamerization associated with tyrosine-mediated repression of gene expression

MP Dixon, RN Pau, GJ Howlett, DE Dunstan, WH Sawyer, BE Davidson

Journal article  |  2002  |  Journal of Biological Chemistry

Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone, clusterin

DM Hatters, MR Wilson, SB Easterbrook-Smith, GJ Howlett

Journal article  |  2002  |  European Journal of Biochemistry

Macromolecular crowding accelerates amyloid formation by human apolipoprotein C-II

DM Hatters, AP Minton, GJ Howlett

Journal article  |  2002  |  Journal of Biological Chemistry

The structural basis for amyloid formation by plasma apolipoproteins: a review

DM Hatters, GJ Howlett

Journal article  |  2002  |  European Biophysics Journal with Biophysics Letters

Size-distribution analysis of proteins by analytical ultracentrifugation: Strategies and application to model systems

P Schuck, MA Perugini, NR Gonzales, GJ Howlett, D Schubert

Journal article  |  2002  |  Biophysical Journal

Biophysical characterization of interactions involving importin-alpha during nuclear import

B Catimel, T Teh, MRM Fontes, IG Jennings, DA Jans, GJ Howlett, EC Nice, B Kobe

Journal article  |  2001  |  Journal of Biological Chemistry

The molecular chaperone, alpha-crystallin, inhibits amyloid formation by apolipoprotein C-II

DM Hatters, RA Lindner, JA Carver, GJ Howlett

Journal article  |  2001  |  Journal of Biological Chemistry

Identification of a determinant of epidermal growth factor receptor ligand-binding specificity using a truncated, high-affinity form of the ectodomain

TC Elleman, T Domagala, NM McKern, M Nerrie, B Lonnqvist, TE Adams, J Lewis, GO Lovrecz, PA Hoyne, KM Richards, GJ Howlett, J Rothacker, RN Jorissen, MZ Lou, TPJ Garrett, AW Burgess, EC Nice, CW Ward

Journal article  |  2001  |  Biochemistry

Sedimentation analysis of novel DNA structures formed by homo-oligonucleotides

DM Hatters, L Wilson, BW Atcliffe, TD Mulhern, N Guzzo-Pernell, GJ Howlett

Journal article  |  2001  |  Biophysical Journal

NMR structure of human apolipoprotein C-II in the presence of sodium dodecyl sulfate

CA MacRaild, DM Hatters, GJ Howlett, PR Gooley

Journal article  |  2001  |  Biochemistry

The interaction of human apolipoprotein C-I with sub-micellar phospholipid

BW Atcliffe, CA MacRaild, PR Gooley, GJ Howlett

Journal article  |  2001  |  European Journal of Biochemistry

Sub-micellar phospholipid accelerates amyloid formation by apolipoprotein C-II

DM Hatters, LJ Lawrence, GJ Howlett

Journal article  |  2001  |  FEBS Letters