Raymond Wong

Using single cell transcriptomics to study the complexity of human retina

Daniel Urrutia-Cabrera, Raymond Ching-Bong Wong

Journal article  |  2020  |  Neural Regeneration Research

Neuronal Reprogramming for Tissue Repair and Neuroregeneration

Roxanne Hsiang-Chi Liou, Thomas L Edwards, Keith R Martin, Raymond Ching-Bong Wong

Journal article  |  2020  |  International Journal of Molecular Sciences

Stem cell and cell reprogramming technology represent a rapidly growing field in regenerative medicine. A number of novel neural r..

Sustained subcutaneous delivery of secretome of human cardiac stem cells promotes cardiac repair following myocardial infarction.

Andrew R Kompa, David W Greening, Anne M Kong, Paul J McMillan, Haoyun Fang, Ritika Saxena, Raymond CB Wong, Jarmon G Lees, Priyadharshini Sivakumaran, Andrew E Newcomb, Bakhos A Tannous, Cameron Kos, Lina Mariana, Thomas Loudovaris, Derek J Hausenloy, Shiang Y Lim

Journal article  |  2020  |  Cardiovascular Research

AIMS: To establish pre-clinical proof of concept that sustained subcutaneous delivery of the secretome of human cardiac stem cells..

Differentiation of Retinal Glial Cells From Human Embryonic Stem Cells by Promoting the Notch Signaling Pathway

Sook Hyun Chung, Weiyong Shen, Kathryn C Davidson, Alice Pebay, Raymond CB Wong, Belinda Yau, Mark Gillies

Journal article  |  2019  |  Frontiers in Cellular Neuroscience

Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina

Fan Li, Sandy SC Hung, Mohd Khairul Nizam Mohd Khalid, Jiang-Hui Wang, Vicki Chrysostomou, Vickie HY Wong, Vikrant Singh, Kristof Wing, Leilei Tu, James A Bender, Alice Pebay, Anna E King, Anthony L Cook, Raymond CB Wong, Bang Bui, Alex W Hewitt, Guei-Sheung Liu

Journal article  |  2019  |  Human Gene Therapy

A single-cell transcriptome atlas of the adult human retina

Samuel W Lukowski, Camden Y Lo, Alexei A Sharov, Quan Nguyen, Lyujie Fang, Sandy SC Hung, Ling Zhu, Ting Zhang, Ulrike Grunert, Tu Nguyen, Anne Senabouth, Jafar S Jabbari, Emily Welby, Jane C Sowden, Hayley S Waugh, Adrienne Mackey, Graeme Pollock, Trevor D Lamb, Peng-Yuan Wang, Alex W Hewitt, et al.

Journal article  |  2019  |  The EMBO Journal

Using transcription factors for direct reprogramming of neurons in vitro

Layal El Wazan, Daniel Urrutia-Cabrera, Raymond Ching-Bong Wong

Journal article  |  2019  |  World Journal of Stem Cells

CRISPR/Cas-mediated base editing of the AMD high-risk Y402H complement factor H variant

Alex W Hewitt, Peter Tran, Mohd Khairul Nizam Mohd Khalid, Alice Pebay, Anthony L Cook, Helena Liang, Raymond Wong, Jamie E Craig, Guei-Sheung Liu, Sandy Hung

Journal article  |  2019  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Potentials of Cellular Reprogramming as a Novel Strategy for Neuroregeneration (vol 12, 460, 2018)

Lyujie Fang, Layal El Wazan, Christine Tan, Nguyen Tu, Sandy SC Hung, Alex W Hewitt, Raymond CB Wong

Journal article  |  2019  |  Frontiers in Cellular Neuroscience

Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant

Thuan Nguyen Tran Minh, Mohd Khairul Nizam Mohd Khalid, Alice Pebay, Anthony L Cook, Helena H Liang, Raymond CB Wong, Jamie E Craig, Guei-Sheung Liu, Sandy S Hung, Alex W Hewitt

Journal article  |  2019  |  MOLECULAR VISION

A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Lyujie Fang, Sandy SC Hung, Jennifer Yek, Layal El Wazan, Nguyen Tu, Shahnaz Khan, Shiang Y Lim, Alex W Hewitt, Raymond CB Wong

Journal article  |  2019  |  Molecular Therapy - Nucleic Acids

Potentials of Cellular Reprogramming as a Novel Strategy for Neuroregeneration

Lyujie Fang, Layal El Wazan, Christine Tan, Nguyen Tu, Sandy SC Hung, Alex W Hewitt, Raymond CB Wong

Journal article  |  2018  |  Frontiers in Cellular Neuroscience

Research progress cellular reprogramming technology and induced pluripotent stem cells for retinal diseases treatment

L Fang, Y Ding, H Liang, RCB Wong

Journal article  |  2018  |  Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology

Generation of human neural retina transcriptome atlas by single cell RNA sequencing

Samuel Lukowski, Camden Lo, Alexei Sharov, Quan Nguyen, Lyujie Fang, Sandy SC Hung, Ling Zhu, Ting Zhang, Tu Nguyen, Anne Senabouth, Jafar Jabbari, Emily Welby, Jane Sowden, Hayley Waugh, Adrienne Mackey, Graeme Pollock, Trevor Lamb, Peng-Yuan Wang, Alex Hewitt, Mark Gillies, et al.

Journal article  |  2018

Summary The retina is a highly specialized neural tissue that senses light and initiates image processing. Although the functional..

Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies

Maciej Daniszewski, Duncan E Crombie, Rachael Henderson, Helena H Liang, Raymond CB Wong, Alex W Hewitt, Alice Pebay

Journal article  |  2018  |  SLAS TECHNOLOGY: Translating Life Sciences Innovation

Using cell reprogramming technology to convert Muller glia into photoreceptors

Raymond Ching Bong Wong, Nguyen Tu, Lucy Lyujie Fang, Camden Lo, Jafar Jabbari, Sandy Hung, Rick Guei-Sheung Liu, Chi D Luu, Mark C Gillies, Alex Hewitt

Conference Proceedings  |  2018  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Topographical Modulation of Pluripotency and Differentiation of Human Embryonic Stem Cells

Peng-Yuan Wang, Shahnaz Khan, Nguyen Tu, Peter Kingshott, Raymond Ching-Bong Wong

Journal article  |  2018  |  IEEE Transactions on Nanotechnology

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells

Ashfaqul Hogue, Priyadharshini Sivakumaran, Simon T Bond, Naomi XY Ling, Anne M Kong, John W Scott, Nadeeka Bandara, Damian Hernandez, Guei-Sheung Liu, Raymond CB Wong, Michael T Ryan, Derek J Hausenloy, Bruce E Kemp, Jonathan S Oakhill, Brian G Drew, Alice Pebay, Shiang Y Lim

Journal article  |  2018  |  Cell Death Discovery

Efficacy and dynamics of self-targeting CRISPR/Cas constructs for gene editing in the retina

Fan Li, Sandy SC Hung, Jiang-Hui Wang, Vicki Chrysostomou, Vickie HY Wong, James Bender, Leilei Tu, Alice Pébay, Anna King, Anthony Cook, Raymond CB Wong, Bang Bui, Alex Hewitt, Guei-Sheung Liu

Journal article  |  2018

ABSTRACT Safe delivery of CRISPR/Cas endonucleases remains one of the major barriers to the widespread application of in vivo geno..

Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor.

Raymond CB Wong, Martin F Pera, Alice Pébay

Journal article  |  2018  |  Methods in molecular biology (Clifton, N.J.)

Human embryonic stem cells (hESCs) have historically been cultivated on feeder layers of primary mouse embryonic fibroblasts (MEF)..

Heterogeneity of mesenchymal and pluripotent stem cell populations grown on nanogrooves and nanopillars

Peng-Yuan Wang, Sheryl Ding, Huseyin Sumer, Raymond Ching-Bong Wong, Peter Kingshott

Journal article  |  2017  |  Journal of Materials Chemistry B

Drusen in patient-derived hiPSC-RPE models of macular dystrophies

Chad A Galloway, Sonal Dalvi, Sandy SC Hung, Leslie A MacDonald, Lisa R Latchney, Raymond CB Wong, Robyn H Guymer, David A Mackey, David S Williams, Mina M Chung, David M Gamm, Alice Pebay, Alex W Hewitt, Ruchira Singh

Journal article  |  2017  |  Proceedings of the National Academy of Sciences

Development of a Modular Automated System for Maintenance and Differentiation of Adherent Human Pluripotent Stem Cells

Duncan E Crombie, Maciej Daniszewski, Helena H Liang, Tejal Kulkarni, Fan Li, Grace E Lidgerwood, Alison Conquest, Damian Hernandez, Sandy S Hung, Katherine P Gill, Elisabeth De Smit, Lisa S Kearns, Linda Clarke, Valentin M Sluch, Xitiz Chamling, Donald J Zack, Raymond CB Wong, Alex W Hewitt, Alice Pebay

Journal article  |  2017  |  SLAS DISCOVERY: Advancing the Science of Drug Discovery

Drug discovery using induced pluripotent stem cell models of neurodegenerative and ocular diseases

Sandy SC Hung, Shahnaz Khan, Camden Y Lo, Alex W Hewitt, Raymond CB Wong

Journal article  |  2017  |  Pharmacology & Therapeutics

Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors

Raymond CB Wong, Sandy S Hung, Stacey Jackson, Vikrant Singh, Shahnaz Khan, Helena H Liang, Lisa S Kearns, Tu Nguyena, Alison Conquest, Maciej Daniszewski, Alex W Hewitt, Alice Pebay

Journal article  |  2017  |  STEM CELL RESEARCH

Neuroregeneration using in vivo cellular reprogramming

Nguyen Tu, Raymond Ching-Bong Wong

Journal article  |  2017  |  Neural Regeneration Research

CONSTRUCTING HUMAN CARDIAC TISSUE FROM PLURIPOTENT STEM CELLS INCLUDING ELECTRICAL STIMULATION

GJ Dusting, D Hernandez, P Sivakumaran, RK Shepherd, Wong CB Raymond, A Pebay, SY Lim

Conference Proceedings  |  2017  |  HYPERTENSION

Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency

Duncan E Crombie, Claire L Curl, Antonia JA Raaijmakers, Priyadharshini Sivakumaran, Tejal Kulkarni, Raymond CB Wong, Itsunari Minami, Marguerite V Evans-Galea, Shiang Y Lim, Lea Delbridge, Louise A Corben, Mirella Dottori, Norio Nakatsuji, Ian A Trounce, Alex W Hewitt, Martin B Delatycki, Martin F Pera, Alice Pebay

Journal article  |  2017  |  AGING-US

Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy

Raymond CB Wong, Shiang Y Lim, Sandy SC Hung, Stacey Jackson, Shahnaz Khan, Nicole J Van Bergen, Elisabeth De Smit, Helena H Liang, Lisa S Kearns, Linda Clarke, David A Mackey, Alex W Hewitt, Ian A Trounce, Alice Pebay

Journal article  |  2017  |  AGING-US

Automated system for maintenance and differentiation of pluripotent stem cells

Alice Pebay, alex HEWITT, Duncan Crombie, RAY Wong, helena Liang, Maciej Daniszewski

Patent  |  2017

Binary colloidal crystals (BCCs) as a feeder-free system to generate human induced pluripotent stem cells (hiPSCs)

Peng-Yuan Wang, Sandy Shen-Chi Hung, Helmut Thissen, Peter Kingshott, Raymond Ching-Bong Wong

Journal article  |  2016  |  Scientific Reports

The use of induced pluripotent stem cells for studying and treating optic neuropathies

Shahnaz Khan, Sandy Shen-Chi Hung, Raymond Ching-Bong Wong

Journal article  |  2016  |  CURRENT OPINION IN ORGAN TRANSPLANTATION

Assessment of pluripotent stem cell clonality and virtual karyotype, of cells from patients with eye disease, using low-density SNP arrays

Alex W Hewitt, Duncan E Crombie, Kathryn Davidson, Helena Liang, Ray Wong, Sandy Shen-Chi Hung, Sook Chung, Alice Pebay

Conference Proceedings  |  2016  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy(aEuro)

Dean G Phelan, David J Anderson, Sara E Howden, Raymond CB Wong, Peter F Hickey, Kate Pope, Gabrielle R Wilson, Alice Pebay, Andrew M Davis, Steven Petrou, Andrew G Elefanty, Edouard G Stanley, Paul A James, Ivan Macciocca, Melanie Bahlo, Michael M Cheung, David J Amor, David A Elliott, Paul J Lockhart

Journal article  |  2016  |  European Heart Journal

Enriched retinal ganglion cells derived from human embryonic stem cells

Katherine P Gill, Sandy SC Hung, Alexei Sharov, Camden Y Lo, Karina Needham, Grace E Lidgerwood, Stacey Jackson, Duncan E Crombie, Bryony A Nayagam, Anthony L Cook, Alex W Hewitt, Alice Pebay, Raymond CB Wong

Journal article  |  2016  |  SCIENTIFIC REPORTS

Poster Session 1

E Andre, E Yaniz-Galende, C Hamilton, GJ Dusting, N Hellen, CE Poulet, M Diez Cunado, AM Smits, V Lowe, D Eckardt, B Du Pre, R Sanz Ruiz, AT Moerkamp, N Tribulova, T Smani, YV Liskova, S Greco, E Guzzolino, D Franco, E Lozano-Velasco, et al.

Journal article  |  2016  |  Cardiovascular Research

Benefits of electrical stimulation on differentiation and maturation of cardiomyocytes from human induced pluripotent stem cells

GJ Dusting, D Hernandez, P Sivakumaran, R Millard, RCB Wong, A Pebay, RK Shepherd, SY Lim

Journal article  |  2016  |  CARDIOVASCULAR RESEARCH

AAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells In Vivo

Sandy SC Hung, Vicki Chrysostomou, Fan Li, Jeremiah KH Lim, Jiang-Hui Wang, Joseph E Powell, Leilei Tu, Maciej Daniszewski, Camden Lo, Raymond C Wong, Jonathan G Crowston, Alice Pebay, Anna E King, Bang V Bui, Guei-Sheung Liu, Alex W Hewitt

Journal article  |  2016  |  Investigative Opthalmology & Visual Science

Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells

Sandy SC Hung, Nicole J Van Bergen, Stacey Jackson, Helena Liang, David A Mackey, Damian Hernandez, Shiang Y Lim, Alex W Hewitt, Ian Trounce, Alice Pebay, Raymond CB Wong

Journal article  |  2016  |  AGING-US

Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Grace E Lidgerwood, Shiang Y Lim, Duncan E Crombie, Ray Ali, Katherine P Gill, Damian Hernandez, Josh Kie, Alison Conquest, Hayley S Waugh, Raymond CB Wong, Helena H Liang, Alex W Hewitt, Kathryn C Davidson, Alice Pebay

Journal article  |  2016  |  STEM CELL REVIEWS AND REPORTS

An Interactive Multimedia Approach to Improving Informed Consent for Induced Pluripotent Stem Cell Research

Tristan McCaughey, Helena H Liang, Christine Chen, Eva Fenwick, Gwyneth Rees, Raymond CB Wong, James C Vickers, Mathew J Summers, Casimir MacGregor, Jamie E Craig, Megan Munsie, Alice Pebay, Alex W Hewitt

Journal article  |  2016  |  Cell Stem Cell

AAV-mediated CRISPR/Cas gene editing of retinal cells in vivo

Sandy SC Hung, Vicki Chrysostomou, Fan Li, Jeremiah KH Lim, Jiang-Hui Wang, Joseph Powell, Leilei Tu, Maciej Daniszewski, Camden Lo, Raymond Wong, Jonathan Crowston, Alice Pébay, Anna King, Bang Bui, Guei-Sheung Liu, Alex Hewitt

Journal article  |  2016

ABSTRACT PURPOSE CRISPR/Cas has recently been adapted to enable efficient editing of the mammalian genome, opening novel avenues f..

Managing Sleep Disturbances in Cirrhosis

Xun Zhao, Philip Wong

Journal article  |  2016  |  Scientifica

Study of Gap Junctions in Human Embryonic Stem Cells.

Alice Pébay, Raymond CB Wong

Journal article  |  2016  |  Methods in Molecular Biology

Gap junctional intercellular communication (GJIC) has been described in different cell types including stem cells and has been inv..

Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Damian Hernandez, Rodney Millard, Priyadharshini Sivakumaran, Raymond CB Wong, Duncan E Crombie, Alex W Hewitt, Helena Liang, Sandy SC Hung, Alice Pebay, Robert K Shepherd, Gregory J Dusting, Shiang Y Lim

Journal article  |  2016  |  STEM CELLS INTERNATIONAL

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Sandy SC Hung, Alice Pebay, Raymond CB Wong

Journal article  |  2015  |  JOVE-JOURNAL OF VISUALIZED EXPERIMENTS

Efficient Generation of Integration-Free Human Induced Pluripotent Stem Cells From Keratinocytes by Simple Transfection of Episomal Vectors

Yulan Piao, Sandy Shen-Chi Hung, Shiang Y Lim, Raymond Ching-Bong Wong, Minoru SH Ko

Journal article  |  2014  |  STEM CELLS Translational Medicine

Methods of Retinal Ganglion Cell Differentiation From Pluripotent Stem Cells

Katherine P Gill, Alex W Hewitt, Kathryn C Davidson, Alice Pebay, Raymond CB Wong

Journal article  |  2014  |  Translational Vision Science & Technology

Advances in Pluripotent and Adult Stem Cells for Eye Research

Gary SL Peh, Raymond CB Wong

Book Chapter  |  2014

Repression of Global Protein Synthesis by Eif1a-Like Genes That Are Expressed Specifically in the Two-Cell Embryos and the Transient Zscan4-Positive State of Embryonic Stem Cells

Sandy SC Hung, Raymond CB Wong, Alexei A Sharov, Yuhki Nakatake, Hong Yu, Minoru SH Ko

Journal article  |  2013  |  DNA Research

Transcriptional regulation of TRKC by SOX2 in human embryonic stem cells

Helen Fong, Raymond Ching-Bong Wong, Peter J Donovan

Journal article  |  2012  |  Stem Cell Research

Maintenance of human embryonic stem cells by sphingosine-1-phosphate and platelet-derived growth factor.

Raymond CB Wong, Martin F Pera, Alice Pébay

Book Chapter  |  2012  |  Methods in Molecular Biology

Embryonic stem cells are pluripotent and capable of indefinite self-renewal in vitro. Human embryonic stem cells (hESC) have gener..

Acute effect of endothelins on intercellular communication of human embryonic stem cells

RCB Wong, KC Davidson, J Leung, MF Pera, A Pébay

Book Chapter  |  2011

A Novel Role for an RNA Polymerase III Subunit POLR3G in Regulating Pluripotency in Human Embryonic Stem Cells

Raymond Ching-Bong Wong, Sara Pollan, Helen Fong, Abel Ibrahim, Ellen L Smith, Mirabelle Ho, Andrew L Laslett, Peter J Donovan

Journal article  |  2011  |  Stem Cells

L1TD1 Is a Marker for Undifferentiated Human Embryonic Stem Cells

Raymond Ching-Bong Wong, Abel Ibrahim, Helen Fong, Noelle Thompson, Leslie F Lock, Peter J Donovan

Journal article  |  2011  |  PLoS One

Non-classical Signalling Mechanisms in Stem Cells

Alice Pebay, Hitesh Peshavariya, Raymond C.B., Gregory J

Book Chapter  |  2011

Molecular mechanism involved in the maintenance of pluripotent stem cells.

Raymond Ching-Bong Wong, Peter J Donovan, Alice Pébay

Book Chapter  |  2011  |  Journal of Stem Cells

The idea of growing human cells in vitro to yield a renewable source of cells for transplantation has captured the imagination of ..

Study of gap junctions in human embryonic stem cells.

Raymond CB Wong, Alice Pébay

Journal article  |  2010  |  Methods in molecular biology (Clifton, N.J.)

Gap junctional intercellular communication (GJIC) has been described in different cell types including stem cells and has been inv..

MOLECULAR MECHANISM INVOLVED IN THE MAINTENANCE OF PLURIPOTENT STEM CELLS

Raymond Ching-Bong Wong, Peter J Donovan, Alice Pebay

Book Chapter  |  2010

Study of Gap Junctions in Human Embryonic Stem Cells

Raymond CB Wong, Alice Pebay

Book Chapter  |  2010  |  Methods in Molecular Biology

A New Feeder-Free Technique to Expand Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

Mark Denham, Jessie Leung, Cheryl Tay, Raymond CB Wong, Peter Donovan, Mirella Dottori, Alice Pebay

Journal article  |  2009  |  The Open Stem Cell Journal

Acute effect of endothelins on intercellular communication of human embryonic stem cells.

Raymond CB Wong, Kathryn C Davidson, Jessie Leung, Martin F Pera, Alice Pébay

Journal article  |  2009  |  Journal of Stem Cells

Endothelin (ET) family comprises three isoforms, ET-1, ET-2 and ET-3 that bind to two receptors ET-A and ET-B. Upon hESC different..

Role of Gap Junctions in Embryonic and Somatic Stem Cells

Raymond CB Wong, Martin F Pera, Alice Pebay

Journal article  |  2008  |  Stem Cell Reviews and Reports

Signaling pathways involved in the maintenance of human embryonic stem cells.

undefined Wong, A PEBAY

Book Chapter  |  2008

Anti-apoptotic effect of sphingosine-1-phosphate and platelet-derived growth factor in human embryonic stem cells

Raymond CB Wong, Irene Tellis, Pegah Jamshidi, Martin Pera, Alice Pebay

Journal article  |  2007  |  Stem Cells and Development

Signaling pathways involved in the maintenance of human embryonic stem cells

RCB Wong, A Pébay

Journal article  |  2006  |  Journal of Stem Cells

Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system

RCB Wong, M Dottori, KLL Koh, LTV Nguyen, MF Pera, A Pebay

Journal article  |  2006  |  Biochemical and Biophysical Research Communications

Essential roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells

A Pebay, RCB Wong, SM Pitson, EJ Wolvetang, GSL Peh, A Filipczyk, KLL Koh, I Tellis, LTV Nguyen, MF Pera

Journal article  |  2005  |  Stem Cells

Presence of functional gap junctions in human embryonic stem cells

RCB Wong, A Pebay, LTV Nguyen, KLL Koh, MF Pera

Journal article  |  2004  |  Stem Cells