Renwick Dobson

Quaternary variations in the structural assembly of N-acetylglucosamine-6-phosphate deacetylase from Pasteurella multocida.

Lavanyaa Manjunath, David Coombes, James Davies, Mugdha Dhurandhar, Vikas R Tiwari, Renwick CJ Dobson, R Sowdhamini, S Ramaswamy, Sucharita Bose

Journal article  |  2020  |  Proteins: Structure, Function, and Bioinformatics

N-acetylglucosamine 6-phosphate deacetylase (NagA) catalyzes the conversion of N-acetylglucosamine-6-phosphate to glucosamine-6-ph..

G-quadruplex structures bind to EZ-Tn5 transposase.

Simone L Cree, Eng Wee Chua, Jennifer Crowther, Renwick CJ Dobson, Martin A Kennedy

Journal article  |  2020  |  Biochimie

Next generation DNA sequencing and analysis of amplicons spanning the pharmacogene CYP2D6 suggested that the Nextera transposase u..

Sub-Angstrom structure of collagen model peptide (GPO)(10) shows a hydrated triple helix with pitch variation and two proline ring conformations

Hironori Suzuki, Deepti Mahapatra, Amanda J Board, Peter J Steel, Jolon M Dyer, Juliet A Gerrard, Renwick CJ Dobson, Celine Valery

Journal article  |  2020  |  Food Chemistry

Collagens are large structural proteins that are prevalent in mammalian connective tissue. Peptides designed to include a glycine-..

Structure-Function Studies of the Antibiotic Target L,L-Diaminopimelate Aminotransferase from Verrucomicrobium spinosum Reveal an Unusual Oligomeric Structure

Anthony W Weatherhead, Jennifer M Crowther, Christopher R Horne, Yanxiang Meng, David Coombes, Michael J Currie, Serena AJ Watkin, Lily E Adams, Anutthaman Parthasarathy, Renwick CJ Dobson, Andre O Hudson

Journal article  |  2020  |  Biochemistry

While humans lack the biosynthetic pathways for meso-diaminopimelate and l-lysine, they are essential for bacterial survival and a..

The structure of the extracellular domains of human interleukin 11? receptor reveals mechanisms of cytokine engagement

Riley D Metcalfe, Kaheina Aizel, Courtney O Zlatic, Paul M Nguyen, Craig J Morton, Daisy Sio-Seng Lio, Heung-Chin Cheng, Renwick CJ Dobson, Michael W Parker, Paul R Gooley, Tracy L Putoczki, Michael DW Griffin

Journal article  |  2020  |  Journal of Biological Chemistry

Interleukin (IL) 11 activates multiple intracellular signaling pathways by forming a complex with its cell surface α-receptor, IL-..

Comparative Molecular Dynamics Simulations Provide Insight Into Antibiotic Interactions: A Case Study Using the Enzyme L,L-Diaminopimelate Aminotransferase (DapL)

Lily E Adams, Patrick Rynkiewicz, Gregory A Babbitt, Jamie S Mortensen, Rachel A North, Renwick CJ Dobson, Andre O Hudson

Journal article  |  2020  |  Frontiers in Molecular Biosciences

The L,L-diaminopimelate aminotransferase (DapL) pathway, a recently discovered variant of the lysine biosynthetic pathway, is an a..

Structure-based mechanism of preferential complex formation by apoptosis signal-regulating kinases

Sarah J Trevelyan, Jodi L Brewster, Abigail E Burgess, Jennifer M Crowther, Antonia L Cadell, Benjamin L Parker, David R Croucher, Renwick CJ Dobson, James M Murphy, Peter D Mace

Journal article  |  2020  |  Science Signaling

Reader response: Menarche, pregnancies, and breastfeeding do not modify long-term prognosis in multiple sclerosis.

Vilija G Jokubaitis, Ruth Dobson

Journal article  |  2020  |  Neurology

The basis for non-canonical ROK family function in the N-acetylmannosamine kinase from the pathogen Staphylococcus aureus

David Coombes, James S Davies, Michael C Newton-Vesty, Christopher R Horne, Thanuja G Setty, Ramaswamy Subramanian, James WB Moir, Rosmarie Friemann, Santosh Panjikar, Michael DW Griffin, Rachel A North, Renwick CJ Dobson

Journal article  |  2020  |  Journal of Biological Chemistry

In environments where glucose is limited, some pathogenic bacteria metabolize host-derived sialic acid as a nutrient source. N-Ace..

Structure-function analyses of alkylhydroperoxidase D from Streptococcus pneumoniae reveal an unusual three-cysteine active site architecture

Yanxiang Meng, Campbell Sheen, Nicholas J Magon, Mark B Hampton, Renwick CJ Dobson

Journal article  |  2020  |  Journal of Biological Chemistry

During aerobic growth, the Gram-positive facultative anaerobe and opportunistic human pathogen Streptococcus pneumoniae generates ..

Comparing the Conformational Stability of Pyruvate Kinase in the Gas Phase and in Solution

Lucienne Nouchikian, Cristina Lento, Katherine Donovan, Renwick Dobson, Derek J Wilson

Journal article  |  2020  |  Journal of the American Society for Mass Spectrometry

Collision induced unfolding (CIU) is increasingly used to characterize protein complexes in the gas phase and is often employed to..

On the catalytic mechanism of bacteriophage endolysins: Opportunities for engineering

Michael J Love, Gayan S Abeysekera, Andrew C Muscroft-Taylor, Craig Billington, Renwick CJ Dobson

Journal article  |  2020  |  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Bacteriophage endolysins have the potential to be a long-term antibacterial replacement for antibiotics. The exogenous application..

Old-Fashioned Technology in the Era of "Bling": Is There a Future for Text Messaging in Health Care?

Jane C Willcox, Rosie Dobson, Robyn Whittaker

Journal article  |  2019  |  Journal of Medical Internet Research

In the quest to discover the next high-technology solution to solve many health problems, proven established technologies are ofte..

On the structure and function of Escherichia coli YjhC: An oxidoreductase involved in bacterial sialic acid metabolism

Christopher R Horne, Laura Kind, James S Davies, Renwick CJ Dobson

Journal article  |  2019  |  Proteins: Structure, Function, and Bioinformatics

Human pathogenic and commensal bacteria have evolved the ability to scavenge host-derived sialic acids and subsequently degrade th..

The fitness challenge of studying molecular adaptation

David Coombes, James WB Moir, Anthony M Poole, Tim F Cooper, Renwick CJ Dobson

Journal article  |  2019  |  Biochemical Society Transactions

Advances in bioinformatics and high-throughput genetic analysis increasingly allow us to predict the genetic basis of adaptive tra..

The First Purification of Functional Proteins from the Unculturable, Genome-Reduced, Bottlenecked alpha-Proteobacterium 'Candidatus Liberibacter solanacearum'

Jenna M Gilkes, Campbell R Sheen, Rebekah A Frampton, Grant R Smith, Renwick CJ Dobson

Journal article  |  2019  |  Phytopathology®

Oxidative cross-linking of calprotectin occurs in vivo, altering its structure and susceptibility to proteolysis

Teagan S Hoskin, Jennifer M Crowther, Jeanette Cheung, Michael J Epton, Peter D Sly, Peter A Elder, Renwick CJ Dobson, Anthony J Kettle, Nina Dickerhof

Journal article  |  2019  |  Redox Biology

Structure-function analyses of two plant meso-diaminopimelate decarboxylase isoforms reveal that active-site gating provides stereochemical control

Jennifer M Crowther, Penelope J Cross, Michael R Oliver, Mary M Leeman, Austin J Bartl, Anthony W Weatherhead, Rachel A North, Katherine A Donovan, Michael DW Griffin, Hironori Suzuki, Andre O Hudson, Muege Kasanmascheff, Renwick CJ Dobson

Journal article  |  2019  |  Journal of Biological Chemistry

Protein-protein crosslinking in food: Proteomic characterisation methods, consequences and applications

Hannah J McKerchar, Stefan Clerens, Renwick CJ Dobson, Jolon M Dyer, Evelyne Maes, Juliet A Gerrard

Journal article  |  2019  |  Trends in Food Science & Technology

The Quest for Novel Antimicrobial Compounds: Emerging Trends in Research, Development, and Technologies

Pavan K Mantravadi, Karunakaran A Kalesh, Renwick CJ Dobson, Andre O Hudson, Anutthaman Parthasarathy

Journal article  |  2019  |  Antibiotics

Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus

James S Davies, David Coombes, Christopher R Horne, F Grant Pearce, Rosmarie Friemann, Rachel A North, Renwick CJ Dobson

Journal article  |  2019  |  FEBS Letters

Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal Insertion Toolbox for Fluorescent Protein Expression in Bacteria

Rudolf O Schlechter, Hyunwoo Jun, Michal Bernach, Simisola Oso, Erica Boyd, Dian A Munoz-Lintz, Renwick CJ Dobson, Daniela M Remus, Mitja NP Remus-Emsermann

Journal article  |  2018  |  Frontiers in Microbiology

Structural and functional analysis of the GABARAP interaction motif (GIM).

Vladimir V Rogov, Alexandra Stolz, Arvind C Ravichandran, Diana O Rios-Szwed, Hironori Suzuki, Andreas Kniss, Frank Löhr, Soichi Wakatsuki, Volker Dötsch, Ivan Dikic, Renwick Cj Dobson, David G McEwan

Journal article  |  2018  |  EMBO reports

The self-association and thermal denaturation of caprine and bovine beta-lactoglobulin

Jennifer M Crowther, Jane R Allison, Grant A Smolenski, Alison J Hodgkinson, Geoffrey B Jameson, Renwick CJ Dobson

Journal article  |  2018  |  European Biophysics Journal

A bidentate Polycomb Repressive-Deubiquitinase complex is required for efficient activity on nucleosomes

Martina Foglizzo, Adam J Middleton, Abigail E Burgess, Jennifer M Crowther, Renwick CJ Dobson, James M Murphy, Catherine L Day, Peter D Mace

Journal article  |  2018  |  Nature Communications

Chromatic bacteria – A broad host-range plasmid and chromosomal insertion toolbox for fluorescent protein expression in bacteria

Rudolf Schlechter, Hyunwoo Jun, Michał Bernach, Simisola Oso, Erica Boyd, Dian Muñoz-Lintz, Renwick Dobson, Daniela Remus, Mitja Remus-Emsermann

Journal article  |  2018

Abstract Differential fluorescent labelling of bacteria has become instrumental for many aspects of microbiological research, such..

The Sodium Sialic Acid Symporter From Staphylococcus aureus Has Altered Substrate Specificity

Rachel A North, Weixiao Y Wahlgren, Daniela M Remus, Mariafrancesca Scalise, Sarah A Kessans, Elm Dunevall, Elin Claesson, Tatiana P Soares da Costa, Matthew A Perugini, S Ramaswamy, Jane R Allison, Cesare Indiveri, Rosmarie Friemann, Renwick CJ Dobson

Journal article  |  2018  |  Frontiers in Chemistry

Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target

Sarah C Atkinson, Con Dogovski, Kathleen Wood, Michael DW Griffin, Michael A Gorman, Lilian Hor, Cyril F Reboul, Ashley M Buckle, Joachim Wuttke, Michael W Parker, Renwick Cj Dobson, Matthew A Perugini

Journal article  |  2018  |  Structure

Crystal structures and kinetic analyses of N-acetylmannosamine-6-phosphate2-epimerases from Fusobacterium nucleatum and Vibrio cholerae

Lavanyaa Manjunath, Sai Rohit Guntupalli, Michael J Currie, Rachel A North, Renwick CJ Dobson, Vinod Nayak, Ramaswamy Subramanian

Journal article  |  2018  |  Acta Crystallographica Section F Structural Biology Communications

Substrate-bound outward-open structure of a Na -coupled sialic acid symporter reveals a new Na site

Weixiao Y Wahlgren, Elin Dunevall, Rachel A North, Aviv Paz, Mariafrancesca Scalise, Paola Bisignano, Johan Bengtsson-Palme, Parveen Goyal, Elin Claesson, Rhawnie Caing-Carlsson, Rebecka Andersson, Konstantinos Beis, Ulf J Nilsson, Anne Farewell, Lorena Pochini, Cesare Indiveri, Michael Grabe, Renwick CJ Dobson, Jeff Abramson, S Ramaswamy, et al.

Journal article  |  2018  |  Nature Communications

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals

Anutthaman Parthasarathy, Penelope J Cross, Renwick CJ Dobson, Lily E Adams, Michael A Savka, Andre O Hudson

Journal article  |  2018  |  Frontiers in Molecular Biosciences

"Just a spoonful of sugar...": import of sialic acid across bacterial cell membranes.

Rachel A North, Christopher R Horne, James S Davies, Daniela M Remus, Andrew C Muscroft-Taylor, Parveen Goyal, Weixiao Yuan Wahlgren, S Ramaswamy, Rosmarie Friemann, Renwick CJ Dobson

Journal article  |  2018  |  Biophysical Reviews

Eukaryotic cell surfaces are decorated with a complex array of glycoconjugates that are usually capped with sialic acids, a large ..

Potential pathogenicity determinants in the genome of 'Candidatus Liberibacter solanacearum', the causal agent of zebra chip disease of potato

Jenna M Gilkes, Rebekah A Frampton, Grant R Smith, Renwick CJ Dobson

Journal article  |  2018  |  Australasian Plant Pathology

Potential for Bacteriophage Endolysins to Supplement or Replace Antibiotics in Food Production and Clinical Care

Michael J Love, Dinesh Bhandari, Renwick CJ Dobson, Craig Billington

Journal article  |  2018  |  Antibiotics

Effects of BeneficialMutations in pykF Gene Vary over Time and across Replicate Populations in a Long-Term Experiment with Bacteria

Fen Peng, Scott Widmann, Andrea Wunsche, Kristina Duan, Katherine A Donovan, Renwick CJ Dobson, Richard E Lenski, Tim F Cooper

Journal article  |  2018  |  Molecular Biology and Evolution

The fitness effects of mutations can depend on the genetic backgrounds in which they occur and thereby influence future opportunit..

Synthesis and incorporation of an advanced lipid peroxidation end-product building block into collagen mimetic peptides

Iman Kavianinia, Sung-Hyun Yang, Harveen Kaur, Paul WR Harris, Renwick CJ Dobson, Antony J Fairbanks, Margaret A Brimble

Journal article  |  2017  |  Chemical Communications

Structural and functional analysis of the GABARAP interaction motif (GIM)

Vladimir V Rogov, Alexandra Stolz, Arvind C Ravichandran, Diana O Rios-Szwed, Hironori Suzuki, Andreas Kniss, Frank Loehr, Soichi Wakatsuki, Volker Doetsch, Ivan Dikic, Renwick CJ Dobson, David G McEwan

Journal article  |  2017  |  EMBO reports

Lysine Biosynthesis in Microorganisms

AO Hudson, MA Savka, FG Pearce, RCJ Dobson

Book Chapter  |  2017

Dihydrodipicolinate Synthase: Structure, Dynamics, Function, and Evolution

F Grant Pearce, Andre O Hudson, Kerry Loomes, Renwick CJ Dobson

Journal article  |  2017  |  Sub-Cellular Biochemistry

Microfluidics for Small-Angle X-ray Scattering

Serena AJ Watkin, Timothy M Ryan, Antonia G Miller, Volker M Nock, F Grant Pearce, Renwick CJ Dobson

Book Chapter  |  2017

Structure and inhibition of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus

Rachel A North, Andrew JA Watson, F Grant Pearce, Andrew C Muscroft-Taylor, Rosmarie Friemann, Antony J Fairbanks, Renwick CJ Dobson

Journal article  |  2016  |  FEBS Letters

The crystal structure of dihydrodipicolinate reductase from the human-pathogenic bacterium Bartonella henselae strain Houston-1 at 2.3 angstrom resolution

Ali R Cala, Maria T Nadeau, Jan Abendroth, Bart L Staker, Alexandra R Reers, Anthony W Weatherhead, Renwick CJ Dobson, Peter J Myler, Andre O Hudson

Journal article  |  2016  |  Acta Crystallographica Section F Structural Biology Communications

Inhibition of Arabidopsis growth by the allelopathic compound azetidine-2-carboxylate is due to the low amino acid specificity of cytosolic prolyl-tRNA synthetase

Jiyeon Lee, Naveen Joshi, Rita Pasini, Renwick CJ Dobson, Jane Allison, Thomas Leustek

Journal article  |  2016  |  The Plant Journal

Avoidance of stochastic RNA interactions can be harnessed to control protein expression levels in bacteria and archaea

Sinan Ugur Umu, Anthony M Poole, Renwick CJ Dobson, Paul P Gardner

Journal article  |  2016  |  eLife

The epigenetic regulator Smchd1 contains a functional GHKL-type ATPase domain

Kelan Chen, Renwick CJ Dobson, Isabelle S Lucet, Samuel N Young, F Grant Pearce, Marnie E Blewitt, James M Murphy

Journal article  |  2016  |  Biochemical Journal

Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery

Sinothai Poen, Yoshio Nakatani, Helen K Opel-Reading, Moritz Lasse, Renwick CJ Dobson, Kurt L Krause

Journal article  |  2016  |  Biochemical Journal

Conformational Dynamics and Allostery in Pyruvate Kinase

Katherine A Donovan, Shaolong Zhu, Peter Liuni, Fen Peng, Sarah A Kessans, Derek J Wilson, Renwick CJ Dobson

Journal article  |  2016  |  Journal of Biological Chemistry

Grappling with anisotropic data, pseudo-merohedral twinning and pseudo-translational noncrystallographic symmetry: a case study involving pyruvate kinase

Katherine A Donovan, Sarah C Atkinson, Sarah A Kessans, Fen Peng, Tim F Cooper, Michael DW Griffin, Geoffrey B Jameson, Renwick CJ Dobson

Journal article  |  2016  |  Acta Crystallographica Section D Structural Biology

Identification and Partial Characterization of a Novel UDP-N-Acetylenolpyruvoylglucosamine Reductase/UDP-N-Acetylmuramate:L-Alanine Ligase Fusion Enzyme from Verrucomicrobium spinosum DSM 4136(T)

Kubra F Naqvi, Delphine Patin, Matthew S Wheatley, Michael A Savka, Renwick CJ Dobson, Han Ming Gan, Helene Barreteau, Didier Blanot, Dominique Mengin-Lecreulx, Andre O Hudson

Journal article  |  2016  |  Frontiers in Microbiology

Cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate synthase from the human pathogenic bacterium Bartonella henselae strain Houston-1 at 2.1 angstrom resolution

Kubra F Naqvi, Bart L Staker, Renwick CJ Dobson, Dmitry Serbzhinskiy, Banumathi Sankaran, Peter J Myler, Andre O Hudson

Journal article  |  2016  |  Acta Crystallographica Section F Structural Biology Communications

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

Daniel J Klionsky, Kotb Abdelmohsen, Akihisa Abe, Md Joynal Abedin, Hagai Abeliovich, Abraham Acevedo Arozena, Hiroaki Adachi, Christopher M Adams, Peter D Adams, Khosrow Adeli, Peter J Adhihetty, Sharon G Adler, Galila Agam, Rajesh Agarwal, Manish K Aghi, Maria Agnello, Patrizia Agostinis, Patricia V Aguilar, Julio Aguirre-Ghiso, Edoardo M Airoldi, et al.

Journal article  |  2016  |  Autophagy

Avoidance of stochastic RNA interactions can be harnessed to control protein expression levels in bacteria and archaea

Sinan Uğur Umu, Anthony Poole, Renwick Dobson, Paul Gardner

Journal article  |  2015

Abstract A critical assumption of gene expression analysis is that mRNA abundances broadly correlate with protein abundance, but t..

Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation

Kelan Chen, Jiang Hu, Darcy L Moore, Ruijie Liu, Sarah A Kessans, Kelsey Breslin, Isabelle S Lucet, Andrew Keniry, Huei San Leong, Clare L Parish, Douglas J Hilton, Richard JLF Lemmers, Silvere M van der Maarel, Peter E Czabotar, Renwick CJ Dobson, Matthew E Ritchie, Graham F Kay, James M Murphy, Marnie E Blewitt

Journal article  |  2015  |  Proceedings of the National Academy of Sciences

New Insights into the Role of T3 Loop in Determining Catalytic Efficiency of GH28 Endo-Polygalacturonases.

Tao Tu, Kun Meng, Huiying Luo, Ossi Turunen, Lujia Zhang, Yanli Cheng, Xiaoyun Su, Rui Ma, Pengjun Shi, Yaru Wang, Peilong Yang, Bin Yao

Journal article  |  2015  |  PLOS ONE

Intramolecular mobility and conformational changes of flexible loops have important roles in the structural and functional integri..

Ultra-high resolution crystal structure of recombinant caprine beta-lactoglobulin

Jennifer M Crowther, Moritz Lasse, Hironori Suzuki, Sarah A Kessans, Trevor S Loo, Gillian E Norris, Alison J Hodgkinson, Geoffrey B Jameson, Renwick CJ Dobson

Journal article  |  2014  |  FEBS Letters

Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death

Joanne M Hildebrand, Maria C Tanzer, Isabelle S Lucet, Samuel N Young, Sukhdeep K Spall, Pooja Sharma, Catia Pierotti, Jean-Marc Garnier, Renwick CJ Dobson, Andrew I Webb, Anne Tripaydonis, Jeffrey J Babon, Mark D Mulcair, Martin J Scanlon, Warren S Alexander, Andrew F Wilks, Peter E Czabotar, Guillaume Lessene, James M Murphy, John Silke

Journal article  |  2014  |  Proceedings of the National Academy of Sciences

L,L-diaminopimelate aminotransferase (DapL): a putative target for the development of narrow-spectrum antibacterial compounds

Alexander J Triassi, Matthew S Wheatley, Michael A Savka, Han Ming Gan, Renwick CJ Dobson, Andre O Hudson

Journal article  |  2014  |  Frontiers in Microbiology

Identification of the bona fide DHDPS from a common plant pathogen

Sarah C Atkinson, Lilian Hor, Con Dogovski, Renwick CJ Dobson, Matthew A Perugini

Journal article  |  2014  |  Proteins: Structure, Function, and Bioinformatics

The structure of human interleukin-11 reveals receptor-binding site features and structural differences from interleukin-6

Tracy L Putoczki, Renwick CJ Dobson, Michael DW Griffin

Journal article  |  2014  |  Acta Crystallographica Section D Biological Crystallography

Fungal DNA Detected in Blood Samples of Patients Who Received Contaminated Methylprednisolone Injections Reveals Increased Complexity of Causative Agents

Yanan Zhao, Emilian Armeanu, Richard DiVerniero, Terri A Lewis, Richard C Dobson, Dimitrios P Kontoyiannis, Emmanuel Roilides, Thomas J Walsh, David S Perlin

Journal article  |  2014  |  Journal of Clinical Microbiology

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine-6-phosphate 2-epimerase from methicillin-resistant Staphylococcus aureus

Rachel A North, Sarah A Kessans, Michael DW Griffin, Andrew JA Watson, Antony J Fairbanks, Renwick CJ Dobson

Journal article  |  2014  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

The purification, crystallization and preliminary X-ray diffraction analysis of two isoforms of meso-diaminopimelate decarboxylase from Arabidopsis thaliana

Michael R Oliver, Jennifer M Crowther, Mary M Leeman, Sarah A Kessans, Rachel A North, Katherine A Donovan, Michael DW Griffin, Hironori Suzuki, Andre O Hudson, Muege Kasanmascheff, Renwick CJ Dobson

Journal article  |  2014  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of N-acetylmannosamine kinase from methicillin-resistant Staphylococcus aureus

Rachel A North, Simona Seizova, Anja Stampfli, Sarah A Kessans, Hironori Suzuki, Michael DW Griffin, Marc Kvansakul, Renwick CJ Dobson

Journal article  |  2014  |  Acta Crystallographica Section F Structural Biology Communications

Destabilization of the Homotetrameric Assembly of 3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate Synthase from the Hyperthermophile Pyrococcus furiosus Enhances Enzymatic Activity

Ali Reza Nazmi, Linley R Schofield, Renwick CJ Dobson, Geoffrey B Jameson, Emily J Parker

Journal article  |  2014  |  Journal of Molecular Biology

Structural Basis of the Autophagy-Related LC3/Atg13 LIR Complex: Recognition and Interaction Mechanism

Hironori Suzuki, Keisuke Tabata, Eiji Morita, Masato Kawasaki, Ryuichi Kato, Renwick CJ Dobson, Tamotsu Yoshimori, Soichi Wakatsuki

Journal article  |  2014  |  Structure

Differential response of orthologous L,L-diaminopimelate aminotransferases (DapL) to enzyme inhibitory antibiotic lead compounds

Shaun MK McKinnie, Eva M Rodriguez-Lopez, John C Vederas, Jennifer M Crowther, Hironori Suzuki, Renwick CJ Dobson, Thomas Leustek, Alexander J Triassi, Matthew S Wheatley, Andre O Hudson

Journal article  |  2014  |  Bioorganic & Medicinal Chemistry

The Pseudokinase MLKL Mediates Necroptosis via a Molecular Switch Mechanism

James M Murphy, Peter E Czabotar, Joanne M Hildebrand, Isabelle S Lucet, Jian-Guo Zhang, Silvia Alvarez-Diaz, Rowena Lewis, Najoua Lalaoui, Donald Metcalf, Andrew I Webb, Samuel N Young, Leila N Varghese, Gillian M Tannahill, Esme C Hatchell, Ian J Majewski, Toru Okamoto, Renwick CJ Dobson, Douglas J Hilton, Jeffrey J Babon, Nicos A Nicola, et al.

Journal article  |  2013  |  Immunity

Biochemical Characterization of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-2,6-diaminopimelate ligase (MurE) from Verrucomicrobium spinosum DSM 4136

Sean E McGroty, Dhivya T Pattaniyil, Delphine Patin, Didier Blanot, Arvind C Ravichandran, Hironori Suzuki, Renwick CJ Dobson, Michael A Savka, Andre O Hudson

Journal article  |  2013  |  PLoS One

Dimerization of Bacterial Diaminopimelate Epimerase Is Essential for Catalysis

Lilian Hor, Renwick CJ Dobson, Matthew T Downton, John Wagner, Craig A Hutton, Matthew A Perugini

Journal article  |  2013  |  JOURNAL OF BIOLOGICAL CHEMISTRY

Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus

Rachel A North, Sarah A Kessans, Sarah C Atkinson, Hironori Suzuki, Andrew JA Watson, Benjamin R Burgess, Lauren M Angley, Andre O Hudson, Arvind Varsani, Michael DW Griffin, Antony J Fairbanks, Renwick CJ Dobson

Journal article  |  2013  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Structural, kinetic and computational investigation of Vitis vinifera DHDPS reveals new insight into the mechanism of lysine-mediated allosteric inhibition

Sarah C Atkinson, Con Dogovski, Matthew T Downton, Peter E Czabotar, Renwick CJ Dobson, Juliet A Gerrard, John Wagner, Matthew A Perugini

Journal article  |  2013  |  Plant Molecular Biology

Engineering allosteric control to an unregulated enzyme by transfer of a regulatory domain

Penelope J Cross, Timothy M Allison, Renwick CJ Dobson, Geoffrey B Jameson, Emily J Parker

Journal article  |  2013  |  Proceedings of the National Academy of Sciences of the United States of America

Discovery of a novel circular single-stranded DNA virus from porcine faeces

Alyssa Sikorski, Gerardo R Argueello-Astorga, Anisha Dayaram, Renwick CJ Dobson, Arvind Varsani

Journal article  |  2013  |  Archives of Virology

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from Agrobacterium tumefaciens

Sarah C Atkinson, Con Dogovski, Renwick CJ Dobson, Matthew A Perugini

Journal article  |  2012  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

The crystal structure of dihydrodipicolinate synthase from Escherichia coli with bound pyruvate and succinic acid semialdehyde: Unambiguous resolution of the stereochemistry of the condensation product

Berin A Boughton, Renwick CJ Dobson, Craig A Hutton

Journal article  |  2012  |  Proteins - Structure, Function, and Bioinformatics

Bovine beta-Lactoglobulin Is Dimeric Under Imitative Physiological Conditions: Dissociation Equilibrium and Rate Constants over the pH Range of 2.5-7.5

Davide Mercadante, Laurence D Melton, Gillian E Norris, Trevor S Loo, Martin AK Williams, Renwick CJ Dobson, Geoffrey B Jameson

Journal article  |  2012  |  Biophysical Journal

Characterisation of the First Enzymes Committed to Lysine Biosynthesis in Arabidopsis thaliana

Michael DW Griffin, Jagan M Billakanti, Akshita Wason, Sabrina Keller, Haydyn DT Mertens, Sarah C Atkinson, Renwick CJ Dobson, Matthew A Perugini, Juliet A Gerrard, Frederick Grant Pearce

Journal article  |  2012  |  PLoS One

Crystal, Solution and In silico Structural Studies of Dihydrodipicolinate Synthase from the Common Grapevine

Sarah C Atkinson, Con Dogovski, Matthew T Downton, F Grant Pearce, Cyril F Reboul, Ashley M Buckle, Juliet A Gerrard, Renwick CJ Dobson, John Wagner, Matthew A Perugini

Journal article  |  2012  |  PLoS One

Structural and Dynamic Requirements for Optimal Activity of the Essential Bacterial Enzyme Dihydrodipicolinate Synthase

CF Reboul, BT Porebski, MDW Griffin, RCJ Dobson, MA Perugini, JA Gerrard, AM Buckle

Journal article  |  2012  |  PLoS Computational Biology

Molecular characterisation of a novel cassava associated circular ssDNA virus

Anisha Dayaram, Allen Opong, Anja Jaeschke, James Hadfield, Marianna Baschiera, Renwick CJ Dobson, Samuel K Offei, Dionne N Shepherd, Darren P Martin, Arvind Varsani

Journal article  |  2012  |  Virus Research

Genomic and biochemical analysis of the diaminopimelate and lysine biosynthesis pathway in Verrucomicrobium spinosum: identification and partial characterization of L,L-diaminopimelate aminotransferase and UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-meso-diaminopimelate ligase

Victoria R Nachar, Francisco C Savka, Sean E McGroty, Katherine A Donovan, Rachel A North, Renwick CJ Dobson, Larry J Buckley, Andre O Hudson

Journal article  |  2012  |  Frontiers in Microbiology

Characterization of monomeric dihydrodipicolinate synthase variant reveals the importance of substrate binding in optimizing oligomerization

F Grant Pearce, Renwick CJ Dobson, Geoffrey B Jameson, Matthew A Perugini, Juliet A Gerrard

Journal article  |  2011  |  Biochimica et Biophysica Acta-Proteins and Proteomics

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera

Sarah C Atkinson, Con Dogovski, Janet Newman, Renwick CJ Dobson, Matthew A Perugini

Journal article  |  2011  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana

Michael DW Griffin, Jagan M Billakanti, Juliet A Gerrard, Renwick CJ Dobson, F Grant Pearce

Journal article  |  2011  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Dual diaminopimelate biosynthesis pathways in Bacteroides fragilis and Clostridium thermocellum

Andre O Hudson, Ayelet Klartag, Charles Gilvarg, Renwick CJ Dobson, Felipe Garbelini Marques, Thomas Leustek

Journal article  |  2011  |  Biochimica et Biophysica Acta-Proteins and Proteomics

A tetrameric structure is not essential for activity in dihydrodipicolinate synthase (DHDPS) from Mycobacterium tuberculosis

Genevieve Evans, Linda Schuldt, Michael DW Griffin, Sean RA Devenish, F Grant Pearce, Matthew A Perugini, Renwick CJ Dobson, Geoffrey B Jameson, Manfred S Weiss, Juliet A Gerrard

Journal article  |  2011  |  Archives of Biochemistry and Biophysics

In silico modeling of the Menkes copper-translocating P-type ATPase 3rd metal binding domain predicts that phosphorylation regulates copper-binding

NA Veldhuis, MJ Kuiper, RCJ Dobson, RB Pearson, J Camakaris

Journal article  |  2011  |  Biometals

L,L-Diaminopimelate Aminotransferase from Chlamydomonas reinhardtii: A Target for Algaecide Development

Renwick CJ Dobson, Irma Giron, Andre O Hudson

Journal article  |  2011  |  PLoS One

Tyrosine Latching of a Regulatory Gate Affords Allosteric Control of Aromatic Amino Acid Biosynthesis

Penelope J Cross, Renwick CJ Dobson, Mark L Patchett, Emily J Parker

Journal article  |  2011  |  Journal of Biological Chemistry

Purification, crystallization, small-angle X-ray scattering and preliminary X-ray diffraction analysis of the SH2 domain of the Csk-homologous kinase

Natalie J Gunn, Michael A Gorman, Renwick CJ Dobson, Michael W Parker, Terrence D Mulhern

Journal article  |  2011  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Crystallization and preliminary X-ray diffraction analysis of L,L-diaminopimelate aminotransferase (DapL) from Chlamydomonas reinhardtii

Andre O Hudson, Irma Giron, Renwick CJ Dobson

Journal article  |  2011  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Development of the procedures for high-yield expression and rapid purification of active recombinant Csk-homologous kinase (CHK): Comparison of the catalytic activities of CHK and CSK

Khai-Chew Chan, Daisy Sio-Seng Lio, Renwick CJ Dobson, Boonyarin Jarasrassamee, Mohammed I Hossain, Aainaa K Roslee, Kim K Ia, Matthew A Perugini, Heung-Chin Cheng

Journal article  |  2010  |  Protein Expression and Purification

Disruption of quaternary structure in Escherichia coli dihydrodipicolinate synthase (DHDPS) generates a functional monomer that is no longer inhibited by lysine

Andrew C Muscroft-Taylor, Ryan J Catchpole, Renwick CJ Dobson, F Grant Pearce, Matthew A Perugini, Juliet A Gerrard

Journal article  |  2010  |  Archives of Biochemistry and Biophysics

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica.

Jacinta M Wubben, Con Dogovski, Renwick CJ Dobson, Rachel Codd, Juliet A Gerrard, Michael W Parker, Matthew A Perugini

Journal article  |  2010  |  Acta crystallographica. Section F, Structural biology and crystallization communications

Dihydrodipicolinate synthase (DHDPS) is an oligomeric enzyme that catalyzes the first committed step of the lysine-biosynthesis pa..

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica

Jacinta M Wubben, Con Dogovski, Renwick CJ Dobson, Rachel Codd, Juliet A Gerrard, Michael W Parker, Matthew A Perugini

Journal article  |  2010  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

How essential is the 'essential' active-site lysine in dihydrodipicolinate synthase?

Tatiana P Soares da Costa, Andrew C Muscroft-Taylor, Renwick CJ Dobson, Sean RA Devenish, Geoffrey B Jameson, Juliet A Gerrard

Journal article  |  2010  |  Biochimie

Substrate-mediated Stabilization of a Tetrameric Drug Target Reveals Achilles Heel in Anthrax

Jarrod E Voss, Stephen W Scally, Nicole L Taylor, Sarah C Atkinson, Michael DW Griffin, Craig A Hutton, Michael W Parker, Malcolm R Alderton, Juliet A Gerrard, Renwick CJ Dobson, Con Dogovski, Matthew A Perugini

Journal article  |  2010  |  Journal of Biological Chemistry

Exploring the dihydrodipicolinate synthase tetramer: How resilient is the dimer-dimer interface?

Michael DW Griffin, Renwick CJ Dobson, Juliet A Gerrard, Matthew A Perugini

Journal article  |  2010  |  Archives of Biochemistry and Biophysics

The quaternary structure of pyruvate kinase type 1 from Escherichia coli at low nanomolar concentrations

Tong Zhu, Michael F Bailey, Lauren M Angley, Timothy F Cooper, Renwick CJ Dobson

Journal article  |  2010  |  Biochimie

Cloning, expression and crystallization of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus

Sudhir Dommaraju, Michael A Gorman, Con Dogovski, F Grant Pearce, Juliet A Gerrard, Renwick CJ Dobson, Michael W Parker, Matthew A Perugini

Journal article  |  2010  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Crystallization and preliminary X-ray diffraction analysis of diaminopimelate epimerase from Escherichia coli

Lilian Hor, Renwick CJ Dobson, Con Dogovski, Craig A Hutton, Matthew A Perugini

Journal article  |  2010  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Characterisation of dihydrodipicolinate synthase (DHDPS) from Bacillus anthracis

LJ Domigan, SW Scally, MJ Fogg, CA Hutton, MA Perugini, RCJ Dobson, AC Muscroft-Taylor, JA Gerrard, SRA Devenish

Journal article  |  2009  |  Biochimica et Biophysica Acta-Proteins and Proteomics

Specificity versus catalytic potency: The role of threonine 44 in Escherichia coli dihydrodipicolinate synthase mediated catalysis

Renwick CJ Dobson, Matthew A Perugini, Geoffrey B Jameson, Juliet A Gerrard

Journal article  |  2009  |  Biochimie

Aromatic residues in the C-terminal helix of human apoC-I mediate phospholipid interactions and particle morphology

Patrick F James, Con Dogovski, Renwick CJ Dobson, Michael F Bailey, Kenneth N Goldie, John A Karas, Denis B Scanlon, Richard AJ O'Hair, Matthew A Perugini

Journal article  |  2009  |  Journal of Lipid Research

The C-terminal domain of Escherichia coli dihydrodipicolinate synthase (DHDPS) is essential for maintenance of quaternary structure and efficient catalysis

Belinda BB Guo, Sean RA Devenish, Renwick CJ Dobson, Andrew C Muscroft-Taylor, Juliet A Gerrard

Journal article  |  2009  |  Biochemical and Biophysical Research Communications

Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate

Sarah C Atkinson, Renwick CJ Dobson, Janet M Newman, Michael A Gorman, Con Dogovski, Michael W Parker, Matthew A Perugini

Journal article  |  2009  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate

Jarrod E Voss, Stephen W Scally, Nicole L Taylor, Con Dogovski, Malcolm R Alderton, Craig A Hutton, Juliet A Gerrard, Michael W Parker, Renwick CJ Dobson, Matthew A Perugini

Journal article  |  2009  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Lysine biosynthesis in bacteria: an unchartered pathway for novel antibiotic design

C Dogovski, S ATKINSON, S DOMMARAJU, L HOR, RC Dobson, CA Hutton, A Gerrard, MAP Perugini

Book Chapter  |  2009

The high-resolution structure of dihydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate

Sean RA Devenish, Juliet A Gerrard, Geoffrey B Jameson, Renwick CJ Dobson

Journal article  |  2008  |  Acta Crystallographica Section F Structural Biology and Crystallization Communications

Conserved main-chain peptide distortions: A proposed role for Ile203 in catalysis by dihydrodipicolinate synthase

Renwick CJ Dobson, Michael DW Griffin, Sean RA Devenish, F Grant Pearce, Craig A Hutton, Juliet A Gerrard, Geoffrey B Jameson, Matthew A Perugini

Journal article  |  2008  |  Protein Science

Irreversible inhibition of dihydrodipicolinate synthase by 4-oxo-heptenedioic acid analogues

Berin A Boughton, Michael DW Griffin, Paul A O'Donnell, Renwick CJ Dobson, Matthew A Perugini, Juliet A Gerrard, Craig A Hutton

Journal article  |  2008  |  Bioorganic & Medicinal Chemistry

Mutating the Tight-Dimer Interface of Dihydrodipicolinate Synthase Disrupts the Enzyme Quaternary Structure: Toward a Monomeric Enzyme

F Grant Pearce, Renwick CJ Dobson, Anke Weber, Laura A Lane, Margaret G McCammon, Marie A Squire, Matthew A Perugini, Geoffrey B Jameson, Carol V Robinson, Juliet A Gerrard

Journal article  |  2008  |  Biochemistry

Structure and evolution of a novel dimeric enzyme from a clinically important bacterial pathogen

Benjamin R Burgess, Renwick CJ Dobson, Michael F Bailey, Sarah C Atkinson, Michael DW Griffin, Geoffrey B Jameson, Michael W Parker, Juliet A Gerrard, Matthew A Perugini

Journal article  |  2008  |  Journal of Biological Chemistry

Evolution of quaternary structure in a homotetrameric enzyme

Michael DW Griffin, Renwick CJ Dobson, F Grant Pearce, Laurence Antonio, Andrew E Whitten, Chu K Liew, Joel P Mackay, Jill Trewhella, Geoffrey B Jameson, Matthew A Perugini, Juliet A Gerrard

Journal article  |  2008  |  Journal of Molecular Biology

Federated repositories of X-ray diffraction images

Steve Androulakis, Jason Schmidberger, Mark A Bate, Ross DeGori, Anthony Beitz, Cyrus Keong, Bob Cameron, Sheena McGowan, Corrine J Porter, Andrew Harrison, Jane Hunter, Jennifer L Martin, Bostjan Kobe, Renwick CJ Dobson, Michael W Parker, James C Whisstock, Joan Gray, Andrew Treloar, David Groenewegen, Neil Dickson, et al.

Journal article  |  2008  |  Acta Crystallographica Section D - Biological Crystallography

Purification, crystallization and preliminary X-ray diffraction studies to near-atomic resolution of dihydrodipicolinate synthase from methicillin-resistant Staphylococcus aureus

Benjamin R Burgess, Renwick CJ Dobson, Con Dogovski, Geoffrey B Jameson, Michael W Parker, Matthew A Perugini

Journal article  |  2008  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Crystal structure and kinetic study of dihydrodipicolinate synthase from Mycobacterium tuberculosis

Georgia Kefala, Genevieve L Evans, Michael DW Griffin, Sean RA Devenish, F Grant Pearce, Matthew A Perugini, Juliet A Gerrard, Manfred S Weiss, Renwick CJ Dobson

Journal article  |  2008  |  Biochemical Journal

The purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Clostridium botulinum

Renwick CJ Dobson, Sarah C Atkinson, Michael A Gorman, Janet M Newman, Michael W Parker, Matthew A Perugini

Journal article  |  2008  |  Acta Crystallographica Section F - Structural Biology and Crystallization Communications

Inhibiting dihydrodipicolinate synthase across species: Towards specificity for pathogens?

Voula Mitsakos, Renwick CJ Dobson, F Grant Pearce, Sean R Devenish, Genevieve L Evans, Benjamin R Burgess, Matthew A Perugini, Juliet A Gerrard, Craig A Hutton

Journal article  |  2008  |  Bioorganic and Medicinal Chemistry Letters

Conformationally constrained diketopimelic acid analogues as inhibitors of dihydrodipicolinate synthase

Berin A Boughton, Renwick CJ Dobson, Juliet A Gerrardc, Craig A Hutton

Journal article  |  2008  |  Bioorganic and Medicinal Chemistry Letters

Role of arginine 138 in the catalysis and regulation of Escherichia coli dihydrodipicolinate synthase

RCJ Dobson, SRA Devenish, LA Turner, VR Clifford, FG Pearce, GB Jameson, JA Gerrard

Journal article  |  2005  |  Biochemistry

The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance

RCJ Dobson, MDW Griffin, GB Jameson, JA Gerrard

Journal article  |  2005  |  Acta Crystallographica Section D Biological Crystallography

Two new irreversible inhibitors of dihydrodipicolinate synthase: diethyl (E,E)-4-oxo-2,5-heptadienedioate and diethyl (E)-4-oxo-2-heptenedioate

JJ Turner, JP Healy, RCJ Dobson, JA Gerrard, CA Hutton

Journal article  |  2005  |  Bioorganic and Medicinal Chemistry Letters

The crystal structure of three site-directed mutants of Escherichia coli dihydrodipicolinate synthase: Further evidence for a catalytic triad

RCJ Dobson, K Valegard, JA Gerrard

Journal article  |  2004  |  Journal of Molecular Biology

Dihydrodipicolinate synthase (DHDPS) from Escherichia coli displays partial mixed inhibition with respect to its first substrate, pyruvate

RCJ Dobson, MDW Griffin, SJ Roberts, JA Gerrard

Journal article  |  2004  |  Biochimie

Dihydrodipicolinate synthase is not inhibited by its substrate,(S)-aspartate beta-semialdehyde

RCJ Dobson, JA Gerrard, FG Pearce

Journal article  |  2004  |  Biochemical Journal

Two complete syntheses of (S)-aspartate semi-aldehyde and demonstration that Delta(2)-tetrahydroisophthalic acid is a non-competitive inhibitor of dihydrodipicolinate synthase

SJ Roberts, JC Morris, RCJ Dobson, CL Baxter, JA Gerrard

Journal article  |  2004  |  Arkivoc

The preparation of (S)-sspartate semi-aldehyde appropriate for use in biochemical studies

SJ Roberts, JC Morris, RCJ Dobson, JA Gerrard

Journal article  |  2003  |  Bioorganic & Medicinal Chemistry Letters