David Gardner

Detrimental actions of Obesity-associated Advanced Glycation End-products (AGEs) on endometrial epithelial cell proliferation are alleviated by antioxidants and are donor-dependent in human endometrial organoids

Jennifer C Hutchison, Jemma Evans, Tracey A Edgell, Guiying Nie, David K Gardner, Lois A Salamonsen

Journal article  |  2023  |  Reproductive BioMedicine Online

Microfluidics facilitating the use of small extracellular vesicles in innovative approaches to male infertility.

Dale M Goss, Steven A Vasilescu, Gavin Sacks, David K Gardner, Majid E Warkiani

Journal article  |  2023  |  Nat Rev Urol

Sperm are transcriptionally and translationally quiescent and, therefore, rely on the seminal plasma microenvironment for function..

Nano-liter perfusion microfluidic device made entirely by two-photon polymerization for dynamic cell culture with easy cell recovery.

Hanna J McLennan, Adam J Blanch, Samuel J Wallace, Lesley J Ritter, Shauna L Heinrich, David K Gardner, Kylie R Dunning, Marty J Gauvin, Allison K Love, Jeremy G Thompson

Journal article  |  2023  |  Sci Rep

Polydimethylsiloxane (PDMS) has been the material of choice for microfluidic applications in cell biology for many years, with rec..

Acetoacetate and ?-hydroxybutyrate reduce mouse embryo viability via differential metabolic and epigenetic mechanisms

Emma G Whatley, Thi T Truong, Alexandra J Harvey, David K Gardner

Journal article  |  2023  |  REPRODUCTIVE BIOMEDICINE ONLINE

RESEARCH QUESTION: Does the ketone acetoacetate (AcAc) alone, or combined with β-hydroxybutyrate (βOHB), impact mouse embryo devel..

Transient Polycomb activity represses developmental genes in growing oocytes.

Ellen G Jarred, Zhipeng Qu, Tesha Tsai, Ruby Oberin, Sigrid Petautschnig, Heidi Bildsoe, Stephen Pederson, Qing-Hua Zhang, Jessica M Stringer, John Carroll, David K Gardner, Maarten Van den Buuse, Natalie A Sims, William T Gibson, David L Adelson, Patrick S Western

Journal article  |  2022  |  Clin Epigenetics

BACKGROUND: Non-genetic disease inheritance and offspring phenotype are substantially influenced by germline epigenetic programmin..

Making and selecting the best embryo in the laboratory.

David K Gardner, Denny Sakkas

Journal article  |  2022  |  Fertil Steril

Over the past 4 decades our ability to maintain a viable human embryo in vitro has improved dramatically, leading to higher implan..

The role of timing in frozen embryo transfer

Sarah Lensen, Daniel Lantsberg, David K Gardner, Azzahra Daniveruszhka Sophian, Namira Wandafiana, Mohan S Kamath

Journal article  |  2022  |  FERTILITY AND STERILITY

The process of implantation is characterized by a complex cross-talk between the endometrium and the blastocyst, with the endometr..

The Blastocyst Creates a Specialized Microenvironment to Promote Endometrial Receptivity and Implantation

Kathryn GURNER, Jemma EVANS, Alexandra J HARVEY, David K GARDNER

Journal article  |  2022  |  Fertility & Reproduction

Background: At the time of implantation, the blastocyst produces a significant amount of lactic acid (LA), creating a microenviron..

Antioxidants in Mouse Embryo Culture and Vitrification Media Maintain an In Vivo Like Gene Expression Profile

Thi T TRUONG, Alexandra J HARVEY, David K GARDNER

Journal article  |  2022  |  Fertility & Reproduction

Background: Embryo culture and cryopreservation causes detrimental effects on blastocyst gene expression. While combined antioxida..

Short-Term Exposure with Long-Term Effects: The Ketone Body, Beta-Hydroxybutyrate, Alters Mouse Embryo Developmental Programming and Reduces Viability

Emma G WHATLEY, Alexandra J HARVEY, David K GARDNER

Journal article  |  2022  |  Fertility & Reproduction

Background: Diet-induced nutritional changes in the maternal reproductive tract stimulate embryonic adaptations to maintain growth..