Prof David Gardner

Understanding how a fertilized oocyte develops into a viable embryo, and then goes onto become a healthy individual has been my lifetime interest. I completed my BSc (Hons) and my PhD, under the supervision of Henry Leese, at the University of York in the UK.

Following a Fellowship at Harvard Medical School, in the laboratory of John Biggers, I moved to Monash Medical Centre to work with Alan Trounson. My work focused on the metabolism and physiology of the preimplantation mammalian embryo, which led to the development of culture systems capable of supporting the embryos of several mammalian species including the human, mouse, cow and sheep. This work also laid the foundations for the generation of the world’s first human embryonic stem cells. In 1997 I moved back to the USA to become the Scientific Director of the Colorado Centre for Reproductive Medicine, where I was able to undertake translational research and further develop our understanding of early human development. In 2007 I returned to Australia to take up the position of Chair of Zoology and have been the Head of Department since 2009.

Find out more about David Gardner's experience

David Gardner's highlights

FEATURED Journal article

Antioxidants increase blastocyst cryosurvival and viability post-vitrification

David Gardner's selected work

Defining The Epigenetic Origins Of Maternally Inherited Disease.

Research Grant

Prospective randomized multicentre comparison on sibling oocytes comparing G-Series media ..

Journal article

Determining The Impacts Of Epigenetic Modifying Drugs On Germline Programming And Offsprin..

Research Grant

Time-lapse imaging: the state of the art†.

Journal article

Development Of An E8-Based Culture Medium For Pluripotent Stem Cell Culture

Research Contracts

Sperm selection methods in the 21st century.

Journal article

Projects

Displaying the 27 most recent projects by David Gardner.

Defining The Epigenetic Origins Of Maternally Inherited Disease.

Epigenetic (non genetic) changes to the DNA in sperm and eggs can alter outcomes in children. Despite the potential for drugs and diet to me..

2018 - 2020

Active

Determining The Impacts Of Epigenetic Modifying Drugs On Germline Programming And Offspring Health

New drugs have been developed that inhibit specific enzymes that regulate epigenetic pathways in cells. These pathways significantly affect ..

2018 - 2020

Active

Analysis Of Embryo Viability (Simple Microfluidic Device For Analysing Cell Culture Media Samples)

2013 - 2013

Completed

Intergenerational Transmission Of Gender-Specific Metabolic Disease For Offspring Born Small: Maternal, Paternal And Embryonic Roles

This proposal addresses the underlying metabolic health consequences of poor embryo and growth of the baby across generations. We will defin..

2013 - 2015

Completed

Intergenerational Transmission Of Gender-Specific Metabolic Disease For Offspring Born Small: Maternal, Paternal And Embryonic Roles

This proposal addresses the underlying metabolic health consequences of poor embryo and growth of the baby across generations. We will defin..

2013 - 2015

Completed

Analysis Of Embryo Viability (Simple Microfluidic Device For Analysing Cell Culture Media Samples)

2012 - 2013

Completed

Intergenerational Transmission Of Metabolic Disease For Offspring Born Small: Maternal, Paternal And Embryonic Roles

2012 - 2013

Completed

Stem Cells Australia (ARC Funding) Using Ipsc-Derived Neuronal Cultures For Drug Discovery In Neurogenetic Epileptic Disorders (2019 Petrou Project - ARC Cash)

Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw ..

2011 - 2019

Completed

Stem Cells Australia

Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw ..

2011 - 2019

Completed

Stem Cells Australia (ARC Funding) Genetic Engineering On Steroids Ci Tanner

Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw ..

2011 - 2019

Completed

Stem Cells Australia

Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw ..

2011 - 2019

Completed

Stem Cells Australia

Despite progress in stem cell research, scientists do not understand how stem cells “decide” what to become. Stem Cells Australia will draw ..

2011 - 2019

Completed

Fabrication Of Microfluidic Devices For The Culture And Analysis Of Gametes And Embryos

2011

Active

Molecular Control Of Embryonic Diapause

Many species can halt growth of the early embryo (diapause). This project will use novel animal models and new proteomics techniques to clar..

2011 - 2015

Completed

Embryo Culture And Analysis Of Viability

2011 - 2013

Completed

Battle Of The Sexes: Can Surroundings Differentially Affect Male And Female Mammalian Preimplantation Embryos

Equality between the sexes is an issue even at conception. The environment surrounding the embryo can bias the development of one sex over a..

2011 - 2014

Completed

Characterisation Of Cumulus Cell Molecular Mediators Of Oocyte Health

Many women are poorly fertile because of poor egg quality due to age, disease and lifestyle. IVF can assist, but requires large doses of hor..

2011 - 2013

Completed

Culture Media For Human Es Cells

2010 - 2011

Completed

Metabolic Profiling Of Human Embryonic Stem Cells.

Stem cell therapies are becoming a commercial reality. Stem cell products have an estimated value of US$ 87 million this year and are pr..

2009 - 2013

Completed

Metabolic Profiling Of Human Embryonic Stem Cells.

Stem cell therapies are becoming a commercial reality. Stem cell products have an estimated value of US$ 87 million this year and are pr..

2009 - 2013

Completed

Showcasing The Tiegs Museum

2009 - 2010

Completed

Showcasing The Tiegs Museum

2009 - 2010

Completed

Vitrolife Collaboration With David Gardner For The Production Of Culture Media And Solutions For Human Assisted Conception / Ivf

Environmental Disruption Of Development

27

Projects

Project Types

Research Grant

Research Contracts

Internal Research Grant

Scholarly Works

Displaying the 361 most recent scholarly works by David Gardner.

Antioxidants increase blastocyst cryosurvival and viability post-vitrification

Thi T Truong, David K Gardner

Journal article | 2020 | Human Reproduction

STUDY QUESTION: What is the effect of antioxidants acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid (A3) in vitrification..

Prospective randomized multicentre comparison on sibling oocytes comparing G-Series media system with antioxidants versus standard G-Series media system

DK Gardner, T Kuramoto, M Tanaka, S Mitzumoto, M Montag, A Yoshida

Journal article | 2020 | Reproductive BioMedicine Online

Time-lapse imaging: the state of the art†.

Raquel Del Gallego, José Remohí, Marcos Meseguer

Journal article | 2019 | Biology of Reproduction

The introduction of time-lapse imaging to clinical in vitro fertilization practice enabled the undisturbed monitoring of embryos t..

Sperm selection methods in the 21st century.

Denis A Vaughan, Denny Sakkas

Journal article | 2019 | Biology of Reproduction

Natural sperm selection in humans is a rigorous process resulting in the highest quality sperm reaching, and having an opportunity..

Clinical application of embryo aneuploidy testing by next-generation sequencing.

Carmen Rubio, Lorena Rodrigo, Carmen Garcia-Pascual, Vanessa Peinado, Inmaculada Campos-Galindo, Sandra Garcia-Herrero, Carlos Simón

Journal article | 2019 | Biology of Reproduction

We review here the evolution in the field of embryo aneuploidy testing over the last 20 years, from the analysis of a subset of ch..

Precision medicine in human reproduction. Special Issue

Carlos Simon, Denny Sakkas, David Gardner, Hilary Critchley

Journal article | 2019 | Biology of Reproduction

Reducing time to pregnancy and facilitating the birth of healthy children through functional analysis of embryo physiology

Laura Ferrick, Yee Shan Lisa Lee, David K Gardner

Journal article | 2019 | Biology of Reproduction

Reprint of: Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer

DK Gardner, M Lane, J Stevens, T Schlenker, WB Schoolcraft

Journal article | 2019 | Fertility and Sterility

25 historic papers: an ASRM 75th birthday gift from Fertility and Sterility

Craig Niederberger, Antonio Pellicer, Carlos Simon, Martin Kathrins, Marc Goldstein, Mark Sigman, Peter N Schlegel, Santiago Munne, David K Gardner, Ana Cobo, Christos Coutifaris, Jacques Donnez, Hugh S Taylor, Linda C Giudice, Bart CJM Fauser, Steven R Lindheim, Zev Rosenwaks, Robert F Casper, Dominique de Ziegler, William E Gibbons, et al.

Journal article | 2019 | Fertility and Sterility

Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells

Jarmon G Lees, Anne M Kong, Yi C Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J Harvey, David K Gardner, Shiang Y Lim

Journal article | 2019 | Stem Cells International

Male Oxidative Stress Infertility (MOSI): Proposed Terminology and Clinical Practice Guidelines for Management of Idiopathic Male Infertility

Ashok Agarwal, Neel Parekh, Manesh Kumar Panner Selvam, Ralf Henkel, Rupin Shah, Sheryl T Homa, Ranjith Ramasamy, Edmund Ko, Kelton Tremellen, Sandro Esteves, Ahmad Majzoub, Juan G Alvarez, David K Gardner, Channa N Jayasena, Jonathan W Ramsay, Chak-Lam Cho, Ramadan Saleh, Denny Sakkas, James M Hotaling, Scott D Lundy, et al.

Journal article | 2019 | The World Journal of Men's Health

Despite advances in the field of male reproductive health, idiopathic male infertility, in which a man has altered semen character..

Metabolomic and Transcriptional Analyses Reveal Atmospheric Oxygen During Human Induced Pluripotent Stem Cell Generation Impairs Metabolic Reprogramming

James Spyrou, David K Gardner, Alexandra J Harvey

Journal article | 2019 | STEM CELLS

The transition to pluripotency invokes profound metabolic restructuring; however, reprogramming is accompanied by the retention of..

Contemporary quality control in the IVF laboratory: heading to the cloud?

David K Gardner, Thomas B Pool

Journal article | 2019 | Reproductive BioMedicine Online

Individual culture and atmospheric oxygen during culture affect mouse preimplantation embryo metabolism and post-implantation development

Rebecca L Kelley, David K Gardner

Journal article | 2019 | Reproductive BioMedicine Online

Culture of the Mouse Preimplantation Embryo.

David K Gardner, Thi T Truong

Book Chapter | 2019 | Methods in Molecular Biology

The first culture media designed specifically to support development of the preimplantation mouse embryo were formulated over 50 y..

Metabolism Is a Key Regulator of Induced Pluripotent Stem Cell Reprogramming

James Spyrou, David K Gardner, Alexandra J Harvey

Journal article | 2019 | Stem Cells International

Oxygen Regulates Human Pluripotent Stem Cell Metabolic Flux

Jarmon G Lees, Timothy S Cliff, Amanda Gammilonghi, James G Ryall, Stephen Dalton, David K Gardner, Alexandra J Harvey

Journal article | 2019 | Stem Cells International

Mitochondrial and glycolytic remodeling during nascent neural differentiation of human pluripotent stem cells

Jarmon G Lees, David K Gardner, Alexandra J Harvey

Journal article | 2018 | Development

Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance

Jessica M Stringer, Samuel C Forster, Zhipeng Qu, Lexie Prokopuk, Moira K O'Bryan, David K Gardner, Stefan J White, David Adelson, Patrick S Western

Journal article | 2018 | BMC Biology

Forty years of IVF

Craig Niederberger, Antonio Pellicer, Jacques Cohen, David K Gardner, Gianpiero D Palermo, Claire L O'Neill, Stephen Chow, Zev Rosenwaks, Ana Cobo, Jason E Swain, William B Schoolcraft, Rene Frydman, Lauren A Bishop, Davora Aharon, Catherine Gordon, Erika New, Alan Decherney, Seang Lin Tan, Richard J Paulson, James M Goldfarb, et al.

Journal article | 2018 | Fertility and Sterility

Physiological oxygen culture reveals retention of metabolic memory in human induced pluripotent stem cells

Alexandra J Harvey, Carmel O'Brien, Jack Lambshead, John R Sheedy, Joy Rathjen, Andrew L Laslett, David K Gardner

Journal article | 2018 | PLOS ONE

Embryonic gene transcription in the spiny mouse (Acomys cahirinus): a new model of embryonic genome activation.

Jared Mamrot, David Gardner, Peter Temple-Smith, Hayley Dickinson

Journal article | 2018

Our understanding of genetic mechanisms driving early embryonic development is primarily based on experiments conducted on mice, h..

Human embryo development and assessment of viability

DK Gardner

Book Chapter | 2018

Antioxidants improve IVF outcome and subsequent embryo development in the mouse

T Truong, DK Gardner

Journal article | 2017 | Human Reproduction

Will noninvasive methods surpass invasive for assessing gametes and embryos?

Tim Sanchez, Emily A Seidler, David K Gardner, Daniel Needleman, Denny Sakkas

Journal article | 2017 | Fertility and Sterility

Addition of interleukin-6 to mouse embryo culture increases blastocyst cell number and influences the inner cell mass to trophectoderm ratio

Rebecca L Kelley, David K Gardner

Journal article | 2017 | Clinical and Experimental Reproductive Medicine

OBJECTIVE: In vitro culture of preimplantation embryos is improved by grouping embryos together in a drop of media. Individually c..

De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)

Jared Mamrot, Roxane Legaie, Stacey J Ellery, Trevor Wilson, Torsten Seemann, David R Powell, David K Gardner, David W Walker, Peter Temple-Smith, Anthony T Papenfuss, Hayley Dickinson

Journal article | 2017 | Scientific Reports

Impact of the IVF laboratory environment on human preimplantation embryo phenotype

DK Gardner, RL Kelley

Journal article | 2017 | Journal of Developmental Origins of Health and Disease

Embryo development and assessment of viability. In Handbook of IVF, 4th Edition

D Gardner, M Montag

Book Chapter | 2017

The new edition of this text admirably fills the need for a primer on the central topics involved in Human In Vitro Fertilization ..

In vitro culture of individual mouse preimplantation embryos: the role of embryo density, microwells, oxygen, timing and conditioned media

Rebecca L Kelley, David K Gardner

Journal article | 2017 | Reproductive BioMedicine Online

Birthweight and the effects of culture media

Jeremy Thompson, Michael Davies, Thomas Pool, Laura Rienzi, Peter Zsolt Nagy, Thorir Hardarson, Denny Sakkas, David Gardner

Journal article | 2017 | Human Reproduction

Foreword

DK Gardner

Book | 2017

Pluripotent Stem Cell Metabolism and Mitochondria: Beyond ATP

Jarmon G Lees, David K Gardner, Alexandra J Harvey

Journal article | 2017 | Stem Cells International

Uterine flushing proteome of the tammar wallaby after reactivation from diapause

Florine C Martin, Ching-Seng Ang, David K Gardner, Marilyn B Renfree, Geoff Shaw

Journal article | 2016 | Reproduction

Src Family Kinases and p38 Mitogen-Activated Protein Kinases Regulate Pluripotent Cell Differentiation in Culture

Boon Siang Nicholas Tan, Joly Kwek, Chong Kum Edwin Wong, Nicholas J Saner, Charlotte Yap, Fernando Felquer, Michael B Morris, David K Gardner, Peter D Rathjen, Joy Rathjen

Journal article | 2016 | PLOS ONE

Placental Growth Factor Is Secreted by the Human Endometrium and Has Potential Important Functions during Embryo Development and Implantation

Natalie K Binder, Jemma Evans, Lois A Salamonsen, David K Gardner, Tu'uhevaha J Kaitu'u-Lino, Natalie J Hannan

Journal article | 2016 | PLOS ONE

Assessment of human embryo development using morphological criteria in an era of time-lapse, algorithms and 'OMICS': is looking good still important?

David K Gardner, Basak Balaban

Journal article | 2016 | Molecular Human Reproduction

Development of a generally applicable morphokinetic algorithm capable of predicting the implantation potential of embryos transferred on Day 3

Bjorn Molt Petersen, Mikkel Boel, Markus Montag, David K Gardner

Journal article | 2016 | Human Reproduction

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

Rebecca L Kelley, David K Gardner

Journal article | 2016 | Reproductive BioMedicine Online

De novo transcriptome assembly for the spiny mouse (Acomys cahirinus)

Jared Mamrot, Roxane Legaie, Stacey Ellery, Trevor Wilson, David Gardner, David Walker, Peter Temple-Smith, Anthony Papenfuss, Hayley Dickinson

Journal article | 2016

Abstract Background: Spiny mice of the genus Acomys are small desert-dwelling rodents that display physiological characteristics n..

Regulation of amino acid transporters in pluripotent cell populations in the embryo and in culture; novel roles for sodium-coupled neutral amino acid transporters

Boon Siang Nicholas Tan, Peter D Rathjen, Alexandra J Harvey, David K Gardner, Joy Rathjen

Journal article | 2016 | Mechanisms of Development

Antioxidants improve mouse preimplantation embryo development and viability

Thi T Truong, Yu May Soh, David K Gardner

Journal article | 2016 | Human Reproduction

H-1-NMR metabolite profiling of abalone digestive gland in response to short-term starvation

John R Sheedy, Sebastien Lachambre, David K Gardner, Robert W Day

Journal article | 2016 | Aquaculture International

The impact of physiological oxygen during culture, and vitrification for cryopreservation, on the outcome of extended culture in human IVF

David K Gardner

Journal article | 2016 | Reproductive BioMedicine Online

Metaboloepigenetic Regulation of Pluripotent Stem Cells

Alexandra J Harvey, Joy Rathjen, David K Gardner

Journal article | 2016 | Stem Cells International

Physiological aspects of oocyte vitrification

Mark G Larman, David K Gardner

Book Chapter | 2016

Oxygen modulates human embryonic stem cell metabolism in the absence of changes in self-renewal

Alexandra J Harvey, Joy Rathjen, Lijia Jackie Yu, David K Gardner

Journal article | 2016 | Reproduction, Fertility and Development

The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction

Petra L Wale, David K Gardner

Journal article | 2016 | Human Reproduction Update

Biomarkers in reproductive medicine: the quest for new answers

Carlos Simon, Denny Sakkas, David K Gardner, Hilary OD Critchley

Journal article | 2015 | Human Reproduction Update

Diagnosis of human preimplantation embryo viability

David K Gardner, Marcos Meseguer, Carmen Rubio, Nathan R Treff

Journal article | 2015 | Human Reproduction Update

Confirmed dioestrus in pseudopregnant mice using vaginal exfoliative cytology improves embryo transfer implantation rate

Jared Mamrot, Mulyoto Pangestu, David Walker, David K Gardner, Hayley Dickinson

Journal article | 2015 | Reproductive BioMedicine Online

Distinct profiles of human embryonic stem cell metabolism and mitochondria identified by oxygen

Jarmon G Lees, Joy Rathjen, John R Sheedy, David K Gardner, Alexandra J Harvey

Journal article | 2015 | REPRODUCTION

Combined parental obesity negatively impacts preimplantation mouse embryo development, kinetics, morphology and metabolism

Bethany J Finger, Alexandra J Harvey, Mark P Green, David K Gardner

Journal article | 2015 | Human Reproduction

Invitro embryo outgrowth is a bioassay of invivo embryo implantation and development

NK Binder, NJ Hannan, DK Gardner

Journal article | 2015 | Asian Pacific Journal of Reproduction

RECOMBINANT ALBUMIN AS A POTENTIAL ALTERNATIVE TO BLOOD-DERIVED ALBUMIN FOR USE IN BLASTOCYST CULTURE: A SIBLING EMBRYO STUDY.

M Murakami, DK Gardner, S Mizumoto, K Tanaka, A Egashira, T Kuramoto

Conference Proceedings | 2015 | Fertility and Sterility

COMBINED ANTIOXIDANTS SIGNFICANTLY INCREASE MOUSE EMBRYO AND FETAL DEVELOPMENT FOLLOWING CULTURE IN ATMOSPHERIC OXYGEN

TT Truong, Y Soh, DK Gardner

Conference Proceedings | 2015 | Fertility and Sterility

Fathers That Are Born Small Program Alterations in the Next-Generation Preimplantation Rat Embryos

Jordanna S Master, George A Thouas, Alexandra J Harvey, John R Sheedy, Natalie J Hannan, David K Gardner, Mary E Wlodek

Journal article | 2015 | The Journal of Nutrition

Male obesity is associated with changed spermatozoa Cox4i1 mRNA level and altered seminal vesicle fluid composition in a mouse model

Natalie K Binder, John R Sheedy, Natalie J Hannan, David K Gardner

Journal article | 2015 | Molecular Human Reproduction

Low female birth weight and advanced maternal age programme alterations in next-generation blastocyst development

Jordanna S Master, George A Thouas, Alexandra J Harvey, John R Sheedy, Natalie J Hannan, David K Gardner, Mary E Wlodek

Journal article | 2015 | REPRODUCTION

Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner

Natalie K Binder, Sally A Beard, Tu'uhevaha J Kaitu'u-Lino, Stephen Tong, Natalie J Hannan, David K Gardner

Journal article | 2015 | REPRODUCTION

Lactate production by the mammalian blastocyst: Manipulating the microenvironment for uterine implantation and invasion?

David K Gardner

Journal article | 2015 | BioEssays

Redox and Anti-Oxidant State Within Cattle Oocytes Following In Vitro Maturation With Bone Morphogenetic Protein 15 and Follicle Stimulating Hormone

Melanie L Sutton-McDowall, Malcolm Purdey, Hannah M Brown, Andrew D Abell, David G Mottershead, Pablo D Cetica, Gabriel C Dalvit, Ewa M Goldys, Robert B Gilchrist, David K Gardner, Jeremy G Thompson

Journal article | 2015 | Molecular Reproduction and Development

Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage

YSL Lee, GA Thouas, DK Gardner

Journal article | 2015 | Human Reproduction

Spatial Asynchronous Transfer of Cleavage-Stage Mouse Embryos to the Uterus Compromises Fetal Development

Kathryn J Walker, Mark P Green, David K Gardner

Journal article | 2015 | Molecular Reproduction and Development

Soluble Ligands and Their Receptors in Human Embryo Development and Implantation

George A Thouas, Francisco Dominguez, Mark P Green, Felipe Vilella, Carlos Simon, David K Gardner

Journal article | 2015 | Endocrine Reviews

Blastocyst metabolism

David K Gardner, Alexandra J Harvey

Journal article | 2015 | Reproduction, Fertility and Development

Endometrial signals improve embryo outcome: functional role of vascular endothelial growth factor isoforms on embryo development and implantation in mice

NK Binder, J Evans, DK Gardner, LA Salamonsen, NJ Hannan

Journal article | 2014 | Human Reproduction

Cryopreservation in ART and concerns with contamination during cryobanking.

Mark G Larman, Shu Hashimoto, Yoshiharu Morimoto, David K Gardner

Journal article | 2014 | Reproductive Medicine and Biology

The cryopreservation of gametes and embryos is vital to numerous fields of reproductive biology, including assisted human reproduc..

Laboratory Procedures for Human In Vitro Fertilization

Basak Balaban, Denny Sakkas, David K Gardner

Journal article | 2014 | Seminars in Reproductive Medicine

Embryo transfer cannot delineate between the maternal pregnancy environment and germ line effects in the transgenerational transmission of disease in rats

Melanie Tran, Linda A Gallo, Alanna N Hanvey, Andrew J Jefferies, Kerryn T Westcott, Luise A Cullen-McEwen, David K Gardner, Karen M Moritz, Mary E Wlodek

Journal article | 2014 | American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Culture environment regulates amino acid turnover and glucose utilisation in human ES cells

Joy Rathjen, Christine Yeo, Charlotte Yap, Boon Siang Nicholas Tan, Peter D Rathjen, David K Gardner

Journal article | 2014 | Reproduction, Fertility and Development

Impact of oxygen concentration on adult murine pre-antral follicle development in vitro and the corresponding metabolic profile

Debra A Gook, DH Edgar, K Lewis, JR Sheedy, DK Gardner

Journal article | 2014 | Molecular Human Reproduction

Ultrarapid vitrification of mouse oocytes and embryos.

Mark G Larman, David K Gardner

Journal article | 2014 | Methods in Molecular Biology

Cryopreservation facilitates long-term storage of gametes and embryos for numerous purposes. For example, cryobanking of unique mo..

Mammalian preimplantation embryo culture.

David K Gardner, Michelle Lane

Book Chapter | 2014 | Methods in Molecular Biology

Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems f..

Culture of Viable Mammalian Embryos In Vitro

DK Gardner, M Lane

Book Chapter | 2013

Oxygen Affects the Ability of Mouse Blastocysts to Regulate Ammonium

Petra L Wale, David K Gardner

Journal article | 2013 | Biology of Reproduction

MATERNAL UTERINE SECRETED MEDIATOR, VEGF, ENHANCES EMBRYO DEVELOPMENT AND IMPLANTATION

Natalie Binder, David Gardner, Natalie Hannan

Conference Proceedings | 2013 | Placenta

MALE AND FEMALE EMBRYOS DIFFER IN THEIR RESPONSE TO OXYGEN CONCENTRATION.

DK Gardner, RL Kelley

Conference Proceedings | 2013 | Fertility and Sterility

Limitations and benefits of morphologic embryo assessment strategies: How far can morphological assessment go in the identification of viable embryos?

D Sakkas, DK Gardner

Book Chapter | 2013

Metabolism of the viable human embryo

DK Gardner

Book Chapter | 2013

Preface

DK Gardner, D Sakkas, E Seli, D Wells

Book | 2013

Microfluidic devices for the analysis of gamete and embryo physiology

GA Thouas, DL Potter, DK Gardner

Book Chapter | 2013

Morphological assessment of blastocyst stage embryos: Types of grading systems and their reported outcomes

B Balaban, DK Gardner

Book Chapter | 2013

Carbohydrate analysis and embryo viability

M Lane, DK Gardner

Book Chapter | 2013

Human gametes and preimplantation embryos: Assessment and diagnosis

DK Gardner, E Seli, D Sakkas, D Wells

Book | 2013

Human and mouse embryonic development, metabolism and gene expression are altered by an ammonium gradient in vitro

DK Gardner, R Hamilton, B McCallie, WB Schoolcraft, MG Katz-Jaffe

Journal article | 2013 | Reproduction

Oxygen regulates in vitro growth and metabolism of preantral ovarian follicles from adult mice

DA Gook, K Riordan, DH Edgar, JR Sheedy, DK Gardner

Conference Proceedings | 2013 | HUMAN REPRODUCTION

Analysis of metabolism to select viable human embryos for transfer

David K Gardner, Petra L Wale

Journal article | 2013 | Fertility and Sterility

The metabolic framework of pluripotent stem cells and potential mechanisms of regulation

Alexandra J Harvey, Joy Rathjen, David K Gardner

Book Chapter | 2013

Paternal Diet-Induced Obesity Retards Early Mouse Embryo Development, Mitochondrial Activity and Pregnancy Health

Natalie K Binder, Natalie J Hannan, David K Gardner

Journal article | 2012 | PLoS One

Metabolic Differences in Bovine Cumulus-Oocyte Complexes Matured In Vitro in the Presence or Absence of Follicle-Stimulating Hormone and Bone Morphogenetic Protein 15

Melanie L Sutton-McDowall, David G Mottershead, David K Gardner, Robert B Gilchrist, Jeremy G Thompson

Journal article | 2012 | Biology of Reproduction

Oxygen Regulates Amino Acid Turnover and Carbohydrate Uptake During the Preimplantation Period of Mouse Embryo Development

Petra L Wale, David K Gardner

Journal article | 2012 | Biology of Reproduction

Textbook of Assisted Reproductive Techniques, Fourth Edition (Two Volume Set)

David K Gardner, Ariel Weissman, Colin M Howles, Zeev Shoham

Book | 2012

As before, methods, protocols, and techniques of choice are presented by eminent international experts. The two volume set include..

Parental diet-induced obesity leads to retarded early mouse embryo development and altered carbohydrate utilisation by the blastocyst

Natalie K Binder, Megan Mitchell, David K Gardner

Journal article | 2012 | Reproduction, Fertility and Development

Extended embryo culture in human IVF

DK Gardner, M Lane

Book Chapter | 2012

Culture systems for the human embryo

DK Gardner, undefined Lane

Book Chapter | 2012

Evaluation of embryo quality: Analysis of morphology and quantification of nutrient utilization and the metabolome

undefined Sakkas, DK Gardner

Book Chapter | 2012

A superovulation protocol for the spiny mouse (Acomys cahirinus)

Rachael Pasco, David K Gardner, David W Walker, Hayley Dickinson

Journal article | 2012 | Reproduction, Fertility and Development

Analysis of Fertility-Related Soluble Mediators in Human Uterine Fluid Identifies VEGF as a Key Regulator of Embryo Implantation

NJ Hannan, P Paiva, KL Meehan, LJF Rombauts, DK Gardner, LA Salamonsen

Journal article | 2011 | Endocrinology

Analysis of global gene expression following mouse blastocyst cryopreservation

MG Larman, MG Katz-Jaffe, B McCallie, JA Filipovits, DK Gardner

Journal article | 2011 | Human Reproduction

Glucose consumption of single post-compaction human embryos is predictive of embryo sex and live birth outcome

David K Gardner, Petra L Wale, Rebecca Collins, Michelle Lane

Journal article | 2011 | Human Reproduction

Effect of hyaluronan-enriched transfer medium on take home baby rate after day 3 and day 5 embryo transfers: a prospective randomized study

B Balaban, K Yakin, B Ata, A Isiklar, B Urman

Conference Proceedings | 2011 | HUMAN REPRODUCTION

Glucose consumption by human embryos on day 4 and day 5 is predictive of pregnancy and sex

DK Gardner, PL Wale, R Collins, M Lane

Conference Proceedings | 2011 | Human Reproduction

Oxygen regulates amino acid utilisation by the preimplantation mouse embryo

PL Wale, DK Gardner

Conference Proceedings | 2011 | HUMAN REPRODUCTION

Salute to a giant ... Robert Geoffrey Edwards

Kamal Ahuja, Fiona Bennett, Jacques Cohen, Ruth Edwards, Huw Pritchard, Ann Silver, Julio Sirlin, John Slee, Dannie Abse, MJ Ashwood-Smith, Barry Bavister, J Michael Bedford, Henning M Beier, Richard Bronson, Bruce Cattanach, Joseph C Daniel, Edwin A Dawes, Malcolm Ferguson-Smith, Richard Gardner, Dave Griffin, et al.

Journal article | 2011 | REPRODUCTIVE BIOMEDICINE ONLINE

The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting.

undefined Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology

Conference Proceedings | 2011 | Human Reproduction

BACKGROUND: Many variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports..

Vitrification with UV-sterilized supercooled air Reply

Mark G Larman, David K Gardner

Journal article | 2011 | Fertility and Sterility

Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting

Basak Balaban, Daniel Brison, Gloria Calderon, James Catt, Joe Conaghan, Lisa Cowan, Thomas Ebner, David Gardner, Thorir Hardarson, Kersti Lundin, M Cristina Magli, David Mortimer, Sharon Mortimer, Santiago Munne, Dominique Royere, Lynette Scott, Johan Smitz, Alan Thornhill, Jonathan van Blerkom, Etienne Van den Abbeel

Journal article | 2011 | Reproductive BioMedicine Online

Vitrification of mouse embryos with super-cooled air

Mark G Larman, David K Gardner

Journal article | 2011 | Fertility and Sterility

Metabolomics

D Sakkas, DK Gardner

Book Chapter | 2011

Embryo metabolism and in vitro culture

DK Gardner

Book Chapter | 2011

Day of embryo transfer

DK Gardner, D Sakkas

Book Chapter | 2011

Human assisted reproductive technology: Future trends in laboratory and clinical practice

DK Gardner, BRMB Rizk, T Falcone

Book | 2011

Analysis of embryo metabolism and the metabolome to identify themost viable embryo within a cohort

DK Gardner

Book Chapter | 2011

Embryo culture in the twenty-first century

MT Johnson, DK Gardner

Book Chapter | 2011

Analysis of embryo metabolism and the metabolome to identify the most viable embryo within a cohort

DK Gardner

Book Chapter | 2011

Metabolomic profiling for selection of the most viable embryos in clinical IVF

GA Thouas, DK Gardner

Journal article | 2010 | Current Women's Health Reviews

Blastocysts from patients with polycystic ovaries exhibit altered transcriptome and secretome

MG Katz-Jaffe, BR McCallie, A Janesch, JA Filipovits, WB Schoolcraft, DK Gardner

Journal article | 2010 | Reproductive BioMedicine Online

Time-lapse analysis of mouse embryo development in oxygen gradients

PL Wale, DK Gardner

Journal article | 2010 | Reproductive BioMedicine Online

VITRIFICATION OF HUMAN AND MOUSE EMBRYOS USING THE RAPID-I (TM).

B Balaban, A Isiklar, B Urman, DK Gardner, MG Larman

Conference Proceedings | 2010 | Fertility and Sterility

Sex-related physiology of the preimplantation embryo

David K Gardner, Mark G Larman, George A Thouas

Journal article | 2010 | Molecular Human Reproduction

Vitrifying mouse oocytes and embryos with super-cooled air

MG Larman, DK Gardner

Conference Proceedings | 2010 | HUMAN REPRODUCTION

Laboratory techniques in IVF

GA Thouas, DK Gardner

Book Chapter | 2010

Maternal enzyme masks the phenotype of mouse embryos lacking dihydrolipoamide dehydrogenase

MT Johnson, P Vang, J Filipovits, DK Gardner

Journal article | 2009 | Reproductive BioMedicine Online

The role of proteomics in defining the human embryonic secretome

MG Katz-Jaffe, S McReynolds, DK Gardner, WB Schoolcraft

Journal article | 2009 | Molecular Human Reproduction

Transcriptome analysis of in vivo and in vitro matured bovine MII oocytes

MG Katz-Jaffe, BR McCallie, KA Preis, J Filipovits, DK Gardner

Journal article | 2009 | Theriogenology

Disruption of Mitochondrial Malate-Aspartate Shuttle Activity in Mouse Blastocysts Impairs Viability and Fetal Growth

Megan Mitchell, Kara S Cashman, David K Gardner, Jeremy G Thompson, Michelle Lane

Journal article | 2009 | Biology of Reproduction

Evaluation and embryo quality: new strategies to facilitate single embryo transfer

undefined Sakkas, D GARDNER

Book Chapter | 2009

Culture systems for the human embryo

D GARDNER, undefined Lane

Book Chapter | 2009

Heterozygosity for a Mutation in the Murine X-Linked Pdha1 Gene Does Not Impact Ovarian Reserve or Folliculogenesis.

Mark T Johnson, Chelsea K Kimball, David K Gardner

Conference Proceedings | 2009 | BIOLOGY OF REPRODUCTION

Embryo culture systems for the human embryo

DKG Gardner, M Lane

Book Chapter | 2009

Impact of cryopreservation on oocyte physiology, metabolism, and the proteome

MG Larman, DK Gardner

Book Chapter | 2009

Textbook of Assisted Reproductive Technologies

Book | 2009

Can proteomics help to shape the future of human assisted conception?

MG Katz-Jaffe, DK Gardner

Journal article | 2008 | Reproductive BioMedicine Online

Noninvasive metabolic profiling using microfluidics for analysis of single preimplantation embryos

John Paul Urbanski, Mark T Johnson, David D Craig, David L Potter, David K Gardner, Todd Thorsen

Journal article | 2008 | Analytical Chemistry

A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation

B Balaban, B Urman, B Ata, A Isiklar, MG Larman, R Harnilton, DK Gardner

Journal article | 2008 | Human Reproduction

Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

Mandy G Katz-Jaffe, Mark G Larman, Courtney B Sheehan, David K Gardner

Journal article | 2008 | Fertility and Sterility

Using Microfluidics for Metabolic Monitoring of Single Embryo Cultures

T Thorsen, JP Urbanski, MT Johnson, DD Craig, DL Potter, DK Gardner

Conference Proceedings | 2008 | Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008

Exposure mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

undefined Katz-Jaffe, M LARMAN, B Sheehan, D GARDNER

Journal article | 2008 | Fertility and Sterility

BLASTOCYST TRANSFER

David K Gardner

Book Chapter | 2008

Management of poor responders: can outcomes be improved with a novel gonadotropin-releasing hormone antagonist/letrozole protocol?

William B Schoolcraft, Eric S Surrey, Debra A Minjarez, John M Stevens, David K Gardner

Journal article | 2008 | Fertility and Sterility

Dissection of culture media for embryos: the most important and less important components and characteristics

David K Gardner

Journal article | 2008 | Reproduction, Fertility and Development

Maintenance of the meiotic spindle during vitrification in human and mouse oocytes

MG Larman, MG Minasi, L Rienzi, DK Gardner

Journal article | 2007 | Reproductive BioMedicine Online

Trophectoderm biopsy and PGS is feasible in frozen blastocyst transfers.

MG Katz-Jaffe, JM Stevens, WG Kearns, DK Gardner, WB Schoolcraft

Conference Proceedings | 2007 | Fertility and Sterility

Embryology in the era of proteomics

MG Katz-Jaffe, DK Gardner

Journal article | 2007 | Theriogenology

Etiology of infertility affects subsequent blastocyst transcriptome.

MG Katz-Jaffe, BR McCallie, JA Filipovits, WB Schoolcraft, DK Gardner

Conference Proceedings | 2007 | Fertility and Sterility

Ammonium build up in culture media alters human embryonic metabolism and gene expression.

DK Gardner, B Hamilton, BR McCallie, J Stevens, WB Schoolcraft, MG Katz-Jaffe

Conference Proceedings | 2007 | Fertility and Sterility

Single embryo transfer vs. dual embryo transfer in women who have previously delivered multiples: Patients prefer risk of multiple births to risk of lower pregnancy rate.

MA Munoz, TN Hickman, LJ McKenzie, JM Stevens, DK Gardner

Conference Proceedings | 2007 | Fertility and Sterility

Development of a microfluidic platform to measure metabolic activity of preimplantation embryos.

JP Urbanski, MT Johnson, DD Craig, DL Potter, DK Gardner, T Thorsen

Conference Proceedings | 2007 | Fertility and Sterility

Effect of in vitro maturation medium on subsequent quantity and quality of developing embryos.

KA Preis, GE Seidel, DK Gardner

Conference Proceedings | 2007 | Fertility and Sterility

a alpha-lipoic acid: An antioxidant that improves embryo development and protects against oxidative stress.

DW Linck, MG Larman, DK Gardner

Conference Proceedings | 2007 | Fertility and Sterility

Vitrification, but not slow freezing, maintains the meiotic spindle of sibling human oocytes

MG Larman, L Rienzi, MG Minasi, DK Gardner

Conference Proceedings | 2007 | HUMAN REPRODUCTION

Vitrification, but not slow freezing, maintains the meiotic spindle of sibling human oocytes

MG Larman, L Rienzi, MG Minasi, DK Gardner

Conference Proceedings | 2007 | HUMAN REPRODUCTION

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo

Mark T Johnson, Edward A Freeman, David K Gardner, Patricia A Hunt

Journal article | 2007 | Biology of Reproduction

Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation

Kimberly A Preis, George E Seidel, David K Gardner

Journal article | 2007 | Molecular Reproduction and Development

Embryo culture medium: which is the best?

Michelle Lane, David K Gardner

Journal article | 2007 | Best Practice and Research in Clinical Obstetrics and Gynaecology

Unique global transcriptome analysis of mouse trophectoderm and inner cell mass.

Mandy Katz-Jaffe, Jessica Filipovits, Blair McCallie, David Gardner

Conference Proceedings | 2007 | Biology of Reproduction

Analysis of oocyte physiology to improve cryopreservation procedures

David K Gardner, Courtney B Sheehan, Laura Rienzi, Mandy Katz-Jaffe, Mark G Larman

Journal article | 2007 | Theriogenology

In Vitro Fertilization: A Practical Approach

D GARDNER

Book | 2007

Noninvasive metabolic assessment of single cells.

David K Gardner

Journal article | 2007 | Methods in Molecular Medicine

Because metabolism is fundamental to the successful functioning of a cell, perturbations in metabolism can signal compromised viab..

Analysis of blastocyst morphology

D GARDNER, J Stevens, CB Sheehan, WB Schoolcraft

Book Chapter | 2007

1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology

MG Larman, MG Katz-Jaffe, CB Sheehan, DK Gardner

Journal article | 2007 | Human Reproduction

Human embryonic development in vitro

D GARDNER

Book Chapter | 2007

In vitro maturation affects global transcriptome analysis.

Blair McCallie, Mandy Katz-Jaffe, David Gardner

Conference Proceedings | 2007 | Biology of Reproduction

Embryo culture systems

D GARDNER, M Lane

Book Chapter | 2007

The CryoLoop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth

Courtney B Sheehan, Michelle Lane, David K Gardner

Journal article | 2006 | Human Reproduction

Relationship between embryonic secretome and chromosomal abnormalities in human IVF.

MG Katz-Jaffe, J Stevens, WG Kearns, DK Gardner, WB Schoolcraft

Conference Proceedings | 2006 | Fertility and Sterility

Maintenance of the meiotic spindle during vitrification in human and mouse oocytes.

MG Larman, L Rienzi, MG Minasi, DK Gardner

Conference Proceedings | 2006 | Fertility and Sterility

Exogenous gonadotropins alter the proteome of metaphase II mouse oocytes.

CB Sheehan, MG Katz-Jaffe, WB Schoolcraft, DK Gardner

Conference Proceedings | 2006 | Fertility and Sterility

Analysis of pregnancy outcome following blastocyst transfer.

DK Gardner, CB Sheehan, J Stevens, D Minjarez, E Surrey, WB Schoolcraft

Conference Proceedings | 2006 | Fertility and Sterility

Maturation conditions affect metabolism and developmental competence of in vitro matured cumulus-enclosed oocytes.

KA Preis, G Seidel, DK Gardner

Conference Proceedings | 2006 | Fertility and Sterility

Analysis of protein expression (secretome) by human and mouse preimplantation embryos

Mandy G Katz-Jaffe, William B Schoolcraft, David K Gardner

Journal article | 2006 | Fertility and Sterility

Protein expression profiles of early embryos - an important step in the right direction: just not quite ready for prime time - Reply

Mandy G Katz-Jaffe, William B Schoolcraft, David K Gardner

Journal article | 2006 | Fertility and Sterility

Improved felid embryo development by group culture is maintained with heterospecific companions

Rebecca E Spindler, Elizabeth G Crichton, Yuksel Agca, Naida Loskutoff, John Critser, David K Gardner, David E Wildt

Journal article | 2006 | Theriogenology

One-step versus two-step culture of mouse preimplantation embryos

David K Gardner, Michelle Lane

Journal article | 2006 | Human Reproduction

Effects of oxygen tension on the establishment and lactate dehydrogenase activity of murine embryonic stem cells

J Gibbons, E Hewitt, DK Gardner

Journal article | 2006 | Cloning and Stem Cells

Choosing between day 3 and day 5 embryo transfers

DK Gardner, B Balaban

Journal article | 2006 | Clinical Obstetrics and Gynecology

Metabolism, protein content, and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate

JR Herrick, M Lane, DK Gardner, E Behboodi, E Memili, S Blash, Y Echelard, RL Krisher

Journal article | 2006 | Molecular Reproduction and Development

Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability

MG Katz-Jaffe, DK Gardner, WB Schoolcraft

Journal article | 2006 | Fertility and Sterility

Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes

MG Larman, CB Sheehan, DK Gardner

Journal article | 2006 | Reproduction

Vitrification of mouse pronuclear oocytes with no direct liquid nitrogen contact

MG Larman, CB Sheehan, DK Gardner

Journal article | 2006 | Reproductive BioMedicine Online

Interpersonal dependency in older adults and the risks of developing mood and mobility problems when receiving care at home

DK Gardner, E Helmes

Journal article | 2006 | Aging & Mental Health

Metabolic regulation of in-vitro-produced bovine embryos. II. Effects of phenazine ethosulfate, sodium azide and 2,4-dinitrophenol during post-compaction development on glucose metabolism and lipid accumulation

Jose Fernando De La Torre-Sanchez, David K Gardner, Kimberly Preis, John Gibbons, George E Seidel

Journal article | 2006 | Reproduction, Fertility and Development

A proteomic analysis of mammalian preimplantation embryonic development

MG Katz-Jaffe, DW Linck, WB Schoolcraft, DK Gardner

Journal article | 2005 | Reproduction

Quality control in human in vitro fertilization

DK Gardner, L Reed, D Linck, C Sheehan, M Lane

Journal article | 2005 | Seminars in Reproductive Medicine

Cryosystem assessment by glucose uptake of murine blastocysts

DL Walker, DK Gardner, M Lane, IS Tummon, DR Session, AR Thornhill

Journal article | 2005 | Reproductive BioMedicine Online

Metabolic markers of developmental competence for in vitro-matured mouse oocytes

KA Preis, G Seidel, DK Gardner

Journal article | 2005 | Reproduction