Matthias Ernst

Longitudinal quantification of mouse gastric tumor organoid viability and growth using luminescence and microscopy.

Riley J Morrow, Matthias Ernst, Ashleigh R Poh

Journal article  |  2023  |  STAR Protoc

Tumor-derived organoids are valuable for testing anti-cancer drugs in vitro, but existing lysis-based protocols for viability meas..

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9.

Anne Huber, Christine Dijkstra, Matthias Ernst, Moritz F Eissmann

Journal article  |  2023  |  STAR Protoc

Gene-of-interest knockout organoids present a powerful and versatile research tool to study a gene's effects on many biological an..

An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice.

Megan O'Brien, Matthias Ernst, Ashleigh R Poh

Journal article  |  2023  |  STAR Protoc

Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the..

Protocol for investigating tertiary lymphoid structures in human and murine fixed tissue sections using Opal™-TSA multiplex immunohistochemistry.

Luke T Quigley, Lokman Pang, Elnaz Tavancheh, Matthias Ernst, Andreas Behren, Jennifer Huynh, Jessica Da Gama Duarte

Journal article  |  2023  |  STAR Protoc

Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols t..

Genotype-Tailored ERK/MAPK Pathway and HDAC Inhibition Rewires the Apoptotic Rheostat to Trigger Colorectal Cancer Cell Death.

Laura J Jenkins, Ian Y Luk, W Douglas Fairlie, Erinna F Lee, Michelle Palmieri, Kael L Schoffer, Tao Tan, Irvin Ng, Natalia Vukelic, Sharon Tran, Janson WT Tse, Rebecca Nightingale, Zakia Alam, Fiona Chionh, George Iatropoulos, Matthias Ernst, Shoukat Afshar-Sterle, Jayesh Desai, Peter Gibbs, Oliver M Sieber, et al.

Journal article  |  2023  |  Mol Cancer Ther

The EGFR/RAS/MEK/ERK signaling pathway (ERK/MAPK) is hyperactivated in most colorectal cancers. A current limitation of inhibitors..

Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry.

Lokman Pang, Matthias Ernst, Jennifer Huynh

Book Chapter  |  2023  |  Methods in Molecular Biology

Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue sec..

Tumor Growth Remains Refractory to Myc Ablation in Host Macrophages.

Riley J Morrow, Amr H Allam, Josh Konecnik, David Baloyan, Christine Dijkstra, Moritz F Eissmann, Saumya P Jacob, Megan O'Brien, Ashleigh R Poh, Matthias Ernst

Journal article  |  2022  |  Cells

Aberrant expression of the oncoprotein c-Myc (Myc) is frequently observed in solid tumors and is associated with reduced overall s..

Abstract A008: DNA methyltransferase 3A promotes inflammation-associated gastric cancer growth and presents a therapy target for gastric cancer

Anne Huber, Christine Dijkstra, Yuba Bhandari, Hariharan Easwaran, Stephen Baylin, Matthias Ernst, Moritz Eissmann

Journal article  |  2022  |  Cancer Research

Abstract Gastric cancer (GC) remains the third leading cause of cancer-related death worldwide. While inflammation ..

Abstract PR007: Intestinal intraepithelial T cells limit colorectal cancer progression

Sonia Ghilas, Dinesh Raghu, Kelly Tran, Yang Liao, Bhupinder Pal, Shoukat Sterle, Christine Dijkstra, Marina Yakou, Pavitha Parathan, John Mariadason, Matthias Ernst, Andreas Behren, Grant Drummond, Wei Shi, Lisa A Mielke

Journal article  |  2022  |  Cancer Research

Abstract Homeostasis in the gut is achieved by impeccable functioning of tissue resident immune cells that reside a..

Abstract B014: Genotype-tailored ERK/MAPK pathway and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell death

Laura J Jenkins, Ian Y Luk, Walter D Fairlie, Erinna F Lee, Michelle Palmieri, Kael L Schoffer, Tao Tan, Irvin Ng, Natalia Vukelic, Sharon Tran, Janson Tse, Rebecca Nightingale, Zakia Alam, Fiona Chionh, George Iatropoulos, Matthias Ernst, Shoukat Afshar-Sterle, Jayesh Desai, Peter Gibbs, Oliver M Sieber, et al.

Journal article  |  2022  |  Cancer Research

Abstract The EGFR/RAS/MEK/ERK signaling pathway (ERK/MAPK) is hyper-activated in most colorectal cancers (CRCs). A ..