Michael Leeming

Phosphomatics: interactive interrogation of substrate-kinase networks in global phosphoproteomics datasets.

Michael G Leeming, Sean O'Callaghan, Luana Licata, Marta Iannuccelli, Prisca Lo Surdo, Elisa Micarelli, Ching-Seng Ang, Shuai Nie, Swati Varshney, Sadia Ameen, Heung-Chin Cheng, Nicholas A Williamson

Journal article  |  2021  |  Bioinformatics

MOTIVATION: Mass spectrometry-based phosphoproteomics can routinely identify and quantify thousands of phosphorylated peptides fro..

Influence of protein ion charge state on 213 nm top-down UVPD

Simon Becher, Huixin Wang, Michael G Leeming, William A Donald, Sven Heiles

Journal article  |  2021  |  ANALYST

Ultraviolet photodissociation (UVPD) is a powerful and rapidly developing method in top-down proteomics. Sequence coverages can ex..

Expanding the allergen repertoire of salmon and catfish

Thimo Ruethers, Aya C Taki, Shaymaviswanathan Karnaneedi, Shuai Nie, Tanja Kalic, Danyi Dai, Sakda Daduang, Michael Leeming, Nicholas A Williamson, Heimo Breiteneder, Sam S Mehr, Sandip D Kamath, Dianne E Campbell, Andreas L Lopata

Journal article  |  2021  |  Allergy

Background Diagnostic tests for fish allergy are hampered by the large number of under-investigated fish species. Four salmon alle..

Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems

Federico Martinez-Seidel, Pipob Suwanchaikasem, Shuai Nie, Michael G Leeming, Alexandre Augusto Pereira Firmino, Nicholas A Williamson, Joachim Kopka, Ute Roessner, Berin A Boughton

Journal article  |  2021  |  FRONTIERS IN PLANT SCIENCE

Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, th..

Nontargeted Identification of Plasma Proteins O-, N-, and S-Transmethylated by O-Methyl Organophosphates

Huixin Wang, Michael G Leeming, Blake J Cochran, James M Hook, Junming Ho, Giang TH Nguyen, Ling Zhong, Claudiu T Supuran, William A Donald

Journal article  |  2020  |  ANALYTICAL CHEMISTRY

Organophosphates (OPs) are used worldwide as pesticides. However, acute and chronic exposure to OPs can cause serious adverse heal..

Protonation isomers of highly charged protein ions can be separated in FAIMS-MS

J Diana Zhang, Micah T Donor, Amber D Rolland, Michael G Leeming, Huixin Wang, Adam J Trevitt, KM Mohibul Kabir, James S Prell, William A Donald

Journal article  |  2020  |  INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

High-field asymmetric waveform ion mobility spectrometry-mass spectrometry (FAIMS-MS) can resolve over an order of magnitude more ..

Type IX Secretion System Cargo Proteins Are Glycosylated at the C Terminus with a Novel Linking Sugar of the Wbp/Vim Pathway

Paul D Veith, Mikio Shoji, Richard AJ O'Hair, Michael G Leeming, Shuai Nie, Michelle D Glew, Gavin E Reid, Koji Nakayama, Eric C Reynolds

Journal article  |  2020  |  MBIO

Porphyromonas gingivalis and Tannerella forsythia use the type IX secretion system to secrete cargo proteins to the cell surface w..

Differential cleavage of viral polypeptides by allotypic variants of granzyme B skews immunity to mouse cytomegalovirus

Vivien R Sutton, Christopher Andoniou, Michael G Leeming, Colin M House, Sally Watt, Sandra Verschoor, Annette Ciccone, Ilia Voskoboinik, Mariapia Degli-Esposti, Joseph A Trapani

Journal article  |  2020  |  BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

We investigated the molecular basis for the remarkably different survival outcomes of mice expressing different alloforms of the p..

HiTIME: An efficient model-selection approach for the detection of unknown drug metabolites in LC-MS data

Michael G Leeming, Andrew P Isaac, Luke Zappia, Richard AJ O'Hair, William A Donald, Bernard J Pope

Journal article  |  2020  |  SOFTWAREX

An Atlas of Phosphorylation and Proteolytic Processing Events During Excitotoxic Neuronal Death Reveals New Therapeutic Opportunities

Sadia Ameen, Antoine Dufour, Iqbal Hossain, Ashfaqul Hoque, Sharelle Sturgeon, Harshal Nandurkar, Dominik Draxler, Robert Medcalf, Mohd Aizuddin Kamaruddin, Isabelle Lucet, Michael Leeming, Dazhi Liu, Amardeep Dhillon, Jet Phey Lim, Hong-Jian Zhu, Laita Bokhari, Carli Roulston, Oded Kleifeld, Ciccotosto Giuseppe, Nicholas Williamson, et al.

Journal article  |  2020

Summary Excitotoxicity, a neuronal death process in neurological disorders, is initiated by over-stimulation of neuronal ionotropi..