Marnie Blewitt

Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal

Kevin X Lau, Elizabeth A Mason, Joshua Kie, David P De Souza, Joachim Kloehn, Dedreia Tull, Malcolm J McConville, Andrew Keniry, Tamara Beck, Marnie E Blewitt, Matthew E Ritchie, Shalin H Naik, Daniela Zalcenstein, Othmar Korn, Shian Su, Irene Gallego Romero, Catrina Spruce, Christopher L Baker, Tracy C McGarr, Christine A Wells, et al.

Journal article  |  2020  |  NATURE COMMUNICATIONS

Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast st..

HBO1 is required for the maintenance of leukaemia stem cells

Laura MacPherson, Juliana Anokye, Miriam M Yeung, Enid YN Lam, Yih-Chih Chan, Chen-Fang Weng, Paul Yeh, Kathy Knezevic, Miriam S Butler, Annabelle Hoegl, Kah-Lok Chan, Marian L Burr, Linden J Gearing, Tracy Willson, Joy Liu, Jarny Choi, Yuqing Yang, Rebecca A Bilardi, Hendrik Falk, Nguyen Nghi, et al.

Journal article  |  2020  |  Nature

Acute myeloid leukaemia (AML) is a heterogeneous disease characterized by transcriptional dysregulation that results in a block in..

Using long-read sequencing to detect imprinted DNA methylation

Scott Gigante, Quentin Gouil, Alexis Lucattini, Andrew Keniry, Tamara Beck, Matthew Tinning, Lavinia Gordon, Chris Woodruff, Terence P Speed, Marnie E Blewitt, Matthew E Ritchie

Journal article  |  2019  |  Nucleic Acids Research

Development of a KDM6b shRNA conditional knock down mouse model

Julia Prier, Marnie Blewitt, Ross Dickins, Stephen Turner

Journal article  |  2019

Abstract Antigen-specific CD8 + T cell differentiation in response to infection is associated with specific changes in the chromat..

Loss of p53 Causes Stochastic Aberrant X-Chromosome Inactivation and Female-Specific Neural Tube Defects

Alex RD Delbridge, Andrew J Kueh, Francine Ke, Natasha M Zamudio, Farrah El-Saafin, Natasha Jansz, Gao-Yuan Wang, Megan Iminitoff, Tamara Beck, Sue Haupt, Yifang Hu, Rose E May, Lachlan Whitehead, Lin Tai, William Chiang, Marco J Herold, Ygal Haupt, Gordon K Smyth, Tim Thomas, Marnie E Blewitt, et al.

Journal article  |  2019  |  Cell Reports

SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite

Camille Dion, Stephane Roche, Camille Laberthonniere, Natacha Broucqsault, Virginie Mariot, Shifeng Xue, Alexandra D Gurzau, Agnieszka Nowak, Christopher T Gordon, Marie-Cecile Gaillard, Claire El-Yazidi, Morgane Thomas, Andree Schlupp-Robaglia, Chantal Missirian, Valerie Malan, Liham Ratbi, Abdelaziz Sefiani, Bernd Wollnik, Bernard Binetruy, Emmanuelle Salort Campana, et al.

Journal article  |  2019  |  Nucleic Acids Research

Smchd1 Targeting to the Inactive X Is Dependent on the Xist-HnrnpK-PRC1 Pathway

Natasha Jansz, Tatyana Nesterova, Andrew Keniry, Megan Iminitoff, Peter F Hickey, Greta Pintacuda, Osamu Masui, Simon Kobelke, Niall Geoghegan, Kelsey A Breslin, Tracy A Willson, Kelly Rogers, Graham F Kay, Archa H Fox, Haruhiko Koseki, Neil Brockdorff, James M Murphy, Marnie E Blewitt

Journal article  |  2018  |  Cell Reports

Using long-read sequencing to detect imprinted DNA methylation

Scott Gigante, Quentin Gouil, Alexis Lucattini, Andrew Keniry, Tamara Beck, Matthew Tinning, Lavinia Gordon, Chris Woodruff, Terence Speed, Marnie Blewitt, Matthew Ritchie

Journal article  |  2018

Systematic variation in the methylation of cytosines at CpG sites plays a critical role in early development of humans and other m..

MBD4 guards against methylation damage and germ line deficiency predisposes to clonal hematopoiesis and early-onset AML

Mathijs A Sanders, Edward Chew, Christoffer Flensburg, Annelieke Zeilemaker, Sarah E Miller, Adil S al Hinai, Ashish Bajel, Bram Luiken, Melissa Rijken, Tamara McIennan, Remco M Hoogenboezem, Francois G Kavelaars, Stefan Froehling, Marnie E Blewitt, Eric M Bindels, Warren S Alexander, Bob Lowenberg, Andrew W Roberts, Peter JM Valk, Ian J Majewski

Journal article  |  2018  |  Blood

Role of SmcHD1 in establishment of epigenetic states required for the maintenance of the X-inactivated state in mice

Yuki Sakakibara, Koji Nagao, Marnie Blewitt, Hiroyuki Sasaki, Chikashi Obuse, Takashi Sado

Journal article  |  2018  |  Development

X inactivation in mammals is regulated by epigenetic modifications. Functional deficiency of SmcHD1 has been shown to cause de-rep..

Smchd1 regulates long-range chromatin interactions on the inactive X chromosome and at Hox clusters

Natasha Jansz, Andrew Keniry, Marie Trussart, Heidi Bildsoe, Tamara Beck, Ian D Tonks, Arne W Mould, Peter Hickey, Kelsey Breslin, Megan Iminitoff, Matthew E Ritchie, Edwina McGlinn, Graham F Kay, James M Murphy, Marnie E Blewitt

Journal article  |  2018  |  Nature Structural & Molecular Biology

FSHD2-and BAMS-associated mutations confer opposing effects on SMCHD1 function

Alexandra D Gurzau, Kelan Chen, Shifeng Xue, Weiwen Dai, Isabelle S Lucet, Thao Nguyen Ly Thanh, Bruno Reversade, Marnie E Blewitt, James M Murphy

Journal article  |  2018  |  Journal of Biological Chemistry

Studying X chromosome inactivation in the single-cell genomic era

Andrew Keniry, Marnie E Blewitt

Journal article  |  2018  |  Biochemical Society Transactions

Long-range chromatin interactions on the inactive X and at Hox clusters are regulated by the non-canonical SMC protein Smchd1

Natasha Jansz, Andrew Keniry, Marie Trussart, Heidi Bildsoe, Tamara Beck, Ian Tonks, Arne Mould, Peter Hickey, Kelsey Breslin, Megan Iminitoff, Matthew Ritchie, Edwina McGlinn, Graham Kay, James Murphy, Marnie Blewitt

Journal article  |  2018

Abstract The regulation of higher order chromatin structure is complex and dynamic; however we do not yet understand the full suit..

Smchd1 haploinsufficiency exacerbates the phenotype of a transgenic FSHD1 mouse model

Jessica C de Greef, Yvonne D Krom, Bianca den Hamer, Lauren Snider, Yosuke Hiramuki, Rob FP van den Akker, Kelsey Breslin, Miha Pakusch, Daniela CF Salvatori, Bram Slutter, Rabi Tawil, Marnie E Blewitt, Stephen J Tapscott, Silvere M van der Maarel

Journal article  |  2018  |  Human Molecular Genetics

Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

Benjamin J Shields, Andrew Keniry, Marnie E Blewitt, Matthew P McCormack

Journal article  |  2018  |  Methods in molecular biology (Clifton, N.J.)

SHRNA SCREEN FOR NOVEL EPIG ENETIC REGULATORS OF Gamma-GLOBIN SILENCING

Betty Kao, Bradley McColl, Shian Su, Matthew Ritchie, L Jamie Gearing, Miha Pakusch, Tracy Willson, Jovana Maksimovic, Alicia Oshlack, Marnie Blewitt, Jim Vadolas

Conference Proceedings  |  2018  |  JOURNAL OF GENE MEDICINE

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Xiaodong Liu, Christian M Nefzger, Fernando J Rossello, Joseph Chen, Anja S Knaupp, Jaber Firas, Ethan Ford, Jahnvi Pflueger, Jacob M Paynter, Hun S Chy, Carmel M O'Brien, Cheng Huang, Ketan Mishra, Margeaux Hodgson-Garms, Natasha Jansz, Sarah M Williams, Marnie E Blewitt, Susan K Nilsson, Ralf B Schittenhelm, Andrew L Laslett, et al.

Journal article  |  2017  |  Nature Methods

Glimma: interactive graphics for gene expression analysis

Shian Su, Charity W Law, Casey Ah-Cann, Marie-Liesse Asselin-Labat, Marnie E Blewitt, Matthew E Ritchie

Journal article  |  2017  |  Bioinformatics

The Epigenetic Regulator SMCHD1 in Development and Disease

Natasha Jansz, Kelan Chen, James M Murphy, Marnie E Blewitt

Journal article  |  2017  |  Trends in Genetics

De novo mutations in SMCHD1 cause Bosma arhinia microphthalmia syndrome and abrogate nasal development

Christopher T Gordon, Shifeng Xue, Goekhan Yigit, Hicham Filali, Kelan Chen, Nadine Rosins, Koh-ichiro Yoshiura, Myriam Oufadem, Tamara J Beck, Ruth McGowan, Alex C Magee, Janine Altmueller, Camille Dion, Holger Thiele, Alexandra D Gurzau, Peter Nuernberg, Dieter Meschede, Wolfgang Muehlbauer, Nobuhiko Okamoto, Vinod Varghese, et al.

Journal article  |  2017  |  Nature Genetics

How do mutations in epigenetic regulators contribute to disease?

K Chen, ME Blewitt

Journal article  |  2017  |  Biochemist

High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS)

Danqing Yin, Matthew E Ritchie, Jafar S Jabbari, Tamara Beck, Marnie E Blewitt, Andrew Keniry

Journal article  |  2016  |  Genomics Data

Functional interdependence of BRD4 and DOT1L in MLL leukemia

Omer Gilan, Enid YN Lam, Isabelle Becher, Dave Lugo, Ester Cannizzaro, Gerard Joberty, Aoife Ward, Meike Wiese, Chun Yew Fong, Sarah Ftouni, Dean Tyler, Kym Stanley, Laura MacPherson, Chen-Fang Weng, Yih-Chih Chan, Margherita Ghisi, David Smil, Christopher Carpenter, Peter Brown, Neil Garton, et al.

Journal article  |  2016  |  Nature Structural & Molecular Biology

The epigenetic regulator Smchd1 contains a functional GHKL-type ATPase domain

Kelan Chen, Renwick CJ Dobson, Isabelle S Lucet, Samuel N Young, F Grant Pearce, Marnie E Blewitt, James M Murphy

Journal article  |  2016  |  Biochemical Journal

Setdb1-mediated H3K9 methylation is enriched on the inactive X and plays a role in its epigenetic silencing

Andrew Keniry, Linden J Gearing, Natasha Jansz, Joy Liu, Aliaksei Z Holik, Peter F Hickey, Sarah A Kinkel, Darcy L Moore, Kelsey Breslin, Kelan Chen, Ruijie Liu, Catherine Phillips, Miha Pakusch, Christine Biben, Julie M Sheridan, Benjamin T Kile, Catherine Carmichael, Matthew E Ritchie, Douglas J Hilton, Marnie E Blewitt

Journal article  |  2016  |  Epigenetics & Chromatin

The hinge domain of the epigenetic repressor Smchd1 adopts an unconventional homodimeric configuration

Kelan Chen, Peter E Czabotar, Marnie E Blewitt, James M Murphy

Journal article  |  2016  |  Biochemical Journal

Transcriptional profiling of the epigenetic regulator Smchd1

Ruijie Liu, Kelan Chen, Natasha Jansz, Marnie E Blewitt, Matthew E Ritchie

Journal article  |  2016  |  Genomics Data

Why weight? Modelling sample and observational level variability improves power in RNA-seq analyses

Ruijie Liu, Aliaksei Z Holik, Shian Su, Natasha Jansz, Kelan Chen, Huei San Leong, Marnie E Blewitt, Marie-Liesse Asselin-Labat, Gordon K Smyth, Matthew E Ritchie

Journal article  |  2015  |  NUCLEIC ACIDS RESEARCH

Polycomb repressive complex 2 component Suz12 is required for hematopoietic stem cell function and lymphopoiesis

Stanley CW Lee, Sarah Miller, Craig Hyland, Maria Kauppi, Marion Lebois, Ladina Di Rago, Donald Metcalf, Sarah A Kinkel, Emma C Josefsson, Marnie E Blewitt, Ian J Majewski, Warren S Alexander

Journal article  |  2015  |  Blood

Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation

Kelan Chen, Jiang Hu, Darcy L Moore, Ruijie Liu, Sarah A Kessans, Kelsey Breslin, Isabelle S Lucet, Andrew Keniry, Huei San Leong, Clare L Parish, Douglas J Hilton, Richard JLF Lemmers, Silvere M van der Maarel, Peter E Czabotar, Renwick CJ Dobson, Matthew E Ritchie, Graham F Kay, James M Murphy, Marnie E Blewitt

Journal article  |  2015  |  Proceedings of the National Academy of Sciences

Repression of Igf1 expression by Ezh2 prevents basal cell differentiation in the developing lung

Laura A Galvis, Aliaksei Z Holik, Kieran M Short, Julie Pasquet, Aaron TL Lun, Marnie E Blewitt, Ian M Smyth, Matthew E Ritchie, Marie-Liesse Asselin-Labat

Journal article  |  2015  |  DEVELOPMENT

Jarid2 regulates hematopoietic stem cell function by acting with polycomb repressive complex 2

Sarah A Kinkel, Roman Galeev, Christoffer Flensburg, Andrew Keniry, Kelsey Breslin, Omer Gilan, Stanley Lee, Joy Liu, Kelan Chen, Linden J Gearing, Darcy L Moore, Warren S Alexander, Mark Dawson, Ian J Majewski, Alicia Oshlack, Jonas Larsson, Marnie E Blewitt

Journal article  |  2015  |  Blood

A comparison of control samples for ChIP-seq of histone modifications

Christoffer Flensburg, Sarah A Kinkel, Andrew Keniry, Marnie E Blewitt, Alicia Oshlack

Journal article  |  2014  |  Frontiers in Genetics

The first mouse mutants of D14Abb1e (Fam208a) show that it is critical for early development

Sarah K Harten, Timothy J Bruxner, Vandhana Bharti, Marnie Blewitt, Nguyen Thi-My-Tam, Emma Whitelaw, Trevor Epp

Journal article  |  2014  |  Mammalian Genome

An ENU mutagenesis screen to identify novel epigenetic modifiers was established in mice carrying a multi-copy GFP transgene, whic..

The polycomb repressive complex 2 governs life and death of peripheral T cells

Yuxia Zhang, Sarah Kinkel, Jovana Maksimovic, Esther Bandala-Sanchez, Maria C Tanzer, Gaetano Naselli, Jian-Guo Zhang, Yifan Zhan, Andrew M Lew, John Silke, Alicia Oshlack, Marnie E Blewitt, Leonard C Harrison

Journal article  |  2014  |  Blood

An in vivo model for analysis of developmental erythropoiesis and globin gene regulation

Bradley McColl, Betty R Kao, Preeyachan Lourthai, Kasey Chan, Hady Wardan, Mark Roosjen, Orane Delagneau, Linden J Gearing, Marnie E Blewitt, Saovaros Svasti, Suthat Fucharoen, Jim Vadolas

Journal article  |  2014  |  FASEB Journal

Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal beta-like globin genes

Mark Roosjen, Bradley McColl, Betty Kao, Linden J Gearing, Marnie E Blewitt, Jim Vadolas

Journal article  |  2014  |  The FASEB Journal

edgeR: a versatile tool for the analysis of shRNA-seq and CRISPR-Cas9 genetic screens.

Zhiyin Dai, Julie M Sheridan, Linden J Gearing, Darcy L Moore, Shian Su, Sam Wormald, Stephen Wilcox, Liam O'Connor, Ross A Dickins, Marnie E Blewitt, Matthew E Ritchie

Journal article  |  2014  |  F1000Research

Pooled library sequencing screens that perturb gene function in a high-throughput manner are becoming increasingly popular in func..

The Use of Mouse Models to Study Epigenetics

Marnie Blewitt, Emma Whitelaw

Journal article  |  2013  |  Cold Spring Harbor Perspectives in Biology

The use of mouse models to study epigenetics.

Marnie Blewitt, Emma Whitelaw

Journal article  |  2013  |  Cold Spring Harbor perspectives in biology

Much of what we know about the role of epigenetics in the determination of phenotype has come from studies of inbred mice. Some un..

Genome-wide DNA methylation analysis identifies hypomethylated genes regulated by FOXP3 in human regulatory T cells

Yuxia Zhang, Jovana Maksimovic, Gaetano Naselli, Junyan Qian, Michael Chopin, Marnie E Blewitt, Alicia Oshlack, Leonard C Harrison

Journal article  |  2013  |  Blood

Polycomb repressive complex 2 (PRC2) suppresses E mu-myc lymphoma

Stanley CW Lee, Belinda Phipson, Craig D Hyland, Huei San Leong, Rhys S Allan, Aaron Lun, Douglas J Hilton, Stephen L Nutt, Marnie E Blewitt, Gordon K Smyth, Warren S Alexander, Ian J Majewski

Journal article  |  2013  |  Blood

Smchd1 regulates a subset of autosomal genes subject to monoallelic expression in addition to being critical for X inactivation

Arne W Mould, Zhenyi Pang, Miha Pakusch, Ian D Tonks, Mitchell Stark, Dianne Carrie, Pamela Mukhopadhyay, Annica Seidel, Jonathan J Ellis, Janine Deakin, Matthew J Wakefield, Lutz Krause, Marnie E Blewitt, Graham F Kay

Journal article  |  2013  |  Epigenetics and Chromatin

Epigenetic Regulator Smchd1 Functions as a Tumor Suppressor

Huei San Leong, Kelan Chen, Yifang Hu, Stanley Lee, Jason Corbin, Miha Pakusch, James M Murphy, Ian J Majewski, Gordon K Smyth, Warren S Alexander, Douglas J Hilton, Marnie E Blewitt

Journal article  |  2013  |  Cancer Research

An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse

Lucia Daxinger, Sarah K Harten, Harald Oey, Trevor Epp, Luke Isbel, Edward Huang, Nadia Whitelaw, Anwyn Apedaile, Anabel Sorolla, Joan Yong, Vandhana Bharti, Joanne Sutton, Alyson Ashe, Zhenyi Pang, Nathan Wallace, Daniel J Gerhardt, Marnie E Blewitt, Jeffrey A Jeddeloh, Emma Whitelaw

Journal article  |  2013  |  Genome Biology

TLR Regulation of SPSB1 Controls Inducible Nitric Oxide Synthase Induction

Rowena S Lewis, Tatiana B Kolesnik, Zhihe Kuang, Akshay A D'Cruz, Marnie E Blewitt, Seth L Masters, Andrew Low, Tracy Willson, Raymond S Norton, Sandra E Nicholson

Journal article  |  2011  |  Journal of Immunology

ChIP-seq analysis reveals distinct H3K27me3 profiles that correlate with transcriptional activity

Matthew D Young, Tracy A Willson, Matthew J Wakefield, Evelyn Trounson, Douglas J Hilton, Marnie E Blewitt, Alicia Oshlack, Ian J Majewski

Journal article  |  2011  |  Nucleic Acids Research

Reduced dosage of the modifiers of epigenetic reprogramming Dnmt1, Dnmt3L, SmcHD1 and Foxo3a has no detectable effect on mouse telomere length in vivo

Amity R Roberts, Marnie E Blewitt, Neil A Youngson, Emma Whitelaw, Suyinn Chong

Journal article  |  2011  |  Chromosoma

SmcHD1, containing a structural-maintenance-of-chromosomes hinge domain, has a critical role in X inactivation

Marnie E Blewitt, Anne-Valerie Gendrel, Zhenyi Pang, Duncan B Sparrow, Nadia Whitelaw, Jeffrey M Craig, Anwyn Apedaile, Douglas J Hilton, Sally L Dunwoodie, Neil Brockdorff, Graham F Kay, Emma Whitelaw

Journal article  |  2008  |  Nature Genetics

Polycomb repressive complex 2 (PRC2) restricts hematopoietic stem cell activity

Ian J Majewski, Marnie E Blewitt, Carolyn A de Graaf, Edward J McManus, Melanie Bahlo, Adrienne A Hilton, Craig D Hyland, Gordon K Smyth, Jason E Corbin, Donald Metcalf, Warren S Alexander, Douglas J Hilton

Journal article  |  2008  |  PLoS Biology

A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development

Alyson Ashe, Daniel K Morgan, Nadia C Whitelaw, Timothy J Bruxner, Nicola K Vickaryous, Liza L Cox, Natalie C Butterfield, Carol Wicking, Marnie E Blewitt, Sarah J Wilkins, Gregory J Anderson, Timothy C Cox, Emma Whitelaw

Journal article  |  2008  |  Genome Biology

Modifiers of epigenetic reprogramming show paternal effects in the mouse

Suyinn Chong, Nicola Vickaryous, Alyson Ashe, Natasha Zamudio, Neil Youngson, Sarah Hemley, Tomas Stopka, Arthur Skoultchi, Jacqui Matthews, Hamish S Scott, David de Kretser, Moira O'Bryan, Marnie Blewitt, Emma Whitelaw

Journal article  |  2007  |  Nature Genetics

Dynamic reprogramming of DNA methylation at an epigenetically sensitive allele in mice

Marnie E Blewitt, Nicola K Vickaryous, Andras Paldi, Haruhiko Koseki, Emma Whitelaw

Journal article  |  2006  |  PLoS Genetics

There is increasing evidence in both plants and animals that epigenetic marks are not always cleared between generations. Incomple..

An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse

ME Blewitt, NK Vickaryous, SJ Hemley, A Ashe, TJ Bruxner, JI Preis, R Arkell, E Whitelaw

Journal article  |  2005  |  Proceedings of the National Academy of Sciences

We have developed a sensitized screen to identify genes involved in gene silencing, using random N-ethyl-N-nitrosourea mutagenesis..

How the mouse got its spots

ME Blewitt, S Chong, E Whitelaw

Journal article  |  2004  |  Trends in Genetics

One's ultimate phenotype is the result of a combination of genotype and environment, and includes a poorly understood component te..

Metastable epialleles in mammals

VK Rakyan, ME Blewitt, R Druker, JI Preis, E Whitelaw

Journal article  |  2002  |  Trends in Genetics

There are some mammalian alleles that display the unusual characteristic of variable expressivity in the absence of genetic hetero..

Phospholipase C-gamma mediates the hydrolysis of phosphatidylinositol, but not of phosphatidylinositol 4,5-bisphoshate, in carbamylcholine-stimulated islets of langerhans

CJ Mitchell, MM Kelly, M Blewitt, JR Wilson, TJ Biden

Journal article  |  2001  |  Journal of Biological Chemistry

In pancreatic islets the activation of phospholipase C (PLC) by the muscarinic receptor agonist carbamyolcholine (carbachol) resul..