Shiang Lim

Sustained subcutaneous delivery of secretome of human cardiac stem cells promotes cardiac repair following myocardial infarction

Andrew R Kompa, David W Greening, Anne M Kong, Paul J McMillan, Haoyun Fang, Ritika Saxena, Raymond CB Wong, Jarmon G Lees, Priyadharshini Sivakumaran, Andrew E Newcomb, Bakhos A Tannous, Cameron Kos, Lina Mariana, Thomas Loudovaris, Derek J Hausenloy, Shiang Y Lim

Journal article  |  2021  |  CARDIOVASCULAR RESEARCH

AIMS: To establish pre-clinical proof of concept that sustained subcutaneous delivery of the secretome of human cardiac stem cells..

A Tissue Engineering Chamber for Continuous Pulsatile Electrical Stimulation of Vascularized Cardiac Tissues In Vivo.

Damián Hernández, Rodney Millard, Anne M Kong, Owen Burns, Priyadharshini Sivakumaran, Robert K Shepherd, Gregory J Dusting, Shiang Y Lim

Journal article  |  2020  |  Bioelectricity

Background: Cardiomyocytes derived from pluripotent stem cells are immature. Maturation of cardiomyocytes is a multifactorial dyna..

New perspectives on the role of Drp1 isoforms in regulating mitochondrial pathophysiology

Ayeshah A Rosdah, William J Smiles, Jonathan S Oakhill, John W Scott, Christopher G Langendorf, Lea MD Delbridge, Jessica K Holien, Shiang Y Lim

Journal article  |  2020  |  PHARMACOLOGY & THERAPEUTICS

Mitochondria are dynamic organelles constantly undergoing fusion and fission. A concerted balance between the process of mitochond..

Amorphous SiO2 nanoparticles promote cardiac dysfunction via the opening of the mitochondrial permeability transition pore in rat heart and human cardiomyocytes

Omar Lozano, Christian Silva-Platas, Hector Chapoy-Villanueva, Baruc E Perez, Jarmon G Lees, Chrishan JA Ramachandra, Flavio F Contreras-Torres, Anay Lazaro-Alfaro, Estefania Luna-Figueroa, Judith Bernal-Ramirez, Aldemar Gordillo-Galeano, Alfredo Benitez, Yuriana Oropeza-Almazan, Elena C Castillo, Poh Ling Koh, Derek J Hausenloy, Shiang Y Lim, Gerardo Garcia-Rivas

Journal article  |  2020  |  PARTICLE AND FIBRE TOXICOLOGY

BACKGROUND: Silica nanoparticles (nanoSiO2) are promising systems that can deliver biologically active compounds to tissues such a..

Cardiomyocyte functional screening: interrogating comparative electrophysiology of high-throughput model cell systems

Simon P Wells, Helen M Waddell, Choon Boon Sim, Shiang Y Lim, Gabriel B Bernasochi, Davor Pavlovic, Paulus Kirchhof, Enzo R Porrello, Lea MD Delbridge, James R Bell

Journal article  |  2019  |  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Cardiac arrhythmias of both atrial and ventricular origin are an important feature of cardiovascular disease. Novel antiarrhythmic..

Generation of a nitric oxide signaling pathway in mesenchymal stem cells promotes endothelial lineage commitment

Nadeeka Bandara, Saliya Gurusinghe, Anne Kong, Geraldine Mitchell, Le-Xin Wang, Shiang Y Lim, Padraig Strappe

Journal article  |  2019  |  JOURNAL OF CELLULAR PHYSIOLOGY

Enhancing differentiation of mesenchymal stem cells (MSCs) to endothelial cells may improve their ability to vascularize tissue an..

Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells

Jarmon G Lees, Anne M Kong, Yi C Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J Harvey, David K Gardner, Shiang Y Lim

Journal article  |  2019  |  STEM CELLS INTERNATIONAL

Human induced pluripotent stem cells (iPSCs) can be differentiated in vitro into bona fide cardiomyocytes for disease modelling an..

Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle

Anne M Kong, Kiryu K Yap, Shiang Y Lim, Diego Marre, Alice Pebay, Yi-wen Gerrand, Jarmon G Lees, Jason A Palmer, Wayne A Morrison, Geraldine M Mitchell

Journal article  |  2019  |  ACTA BIOMATERIALIA

Tissue flaps are used to cover large/poorly healing wounds, but involve complex surgery and donor site morbidity. In this study a ..

A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Lyujie Fang, Sandy SC Hung, Jennifer Yek, Layal El Wazan, Nguyen Tu, Shahnaz Khan, Shiang Y Lim, Alex W Hewitt, Raymond CB Wong

Journal article  |  2019  |  MOLECULAR THERAPY-NUCLEIC ACIDS

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiologi..

Methods for Assessing Scaffold Vascularization In Vivo

Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M Mitchell, Shiang Y Lim, Guei-Sheung Liu

Journal article  |  2019  |  SKIN TISSUE ENGINEERING: METHODS AND PROTOCOLS

The success of tissue engineering hinges on the rapid and sufficient vascularization of the neotissue. For efficient vascular netw..