Shiang Lim

Sustained subcutaneous delivery of secretome of human cardiac stem cells promotes cardiac repair following myocardial infarction.

Andrew R Kompa, David W Greening, Anne M Kong, Paul J McMillan, Haoyun Fang, Ritika Saxena, Raymond CB Wong, Jarmon G Lees, Priyadharshini Sivakumaran, Andrew E Newcomb, Bakhos A Tannous, Cameron Kos, Lina Mariana, Thomas Loudovaris, Derek J Hausenloy, Shiang Y Lim

Journal article  |  2020  |  Cardiovascular Research

AIMS: To establish pre-clinical proof of concept that sustained subcutaneous delivery of the secretome of human cardiac stem cells..

Generation of a nitric oxide signaling pathway in mesenchymal stem cells promotes endothelial lineage commitment

Nadeeka Bandara, Saliya Gurusinghe, Anne Kong, Geraldine Mitchell, Le-Xin Wang, Shiang Y Lim, Padraig Strappe

Journal article  |  2019  |  Journal of Cellular Physiology

Mitochondrial Fusion by M1 Promotes Embryoid Body Cardiac Differentiation of Human Pluripotent Stem Cells

Jarmon G Lees, Anne M Kong, Yi C Chen, Priyadharshini Sivakumaran, Damian Hernandez, Alice Pebay, Alexandra J Harvey, David K Gardner, Shiang Y Lim

Journal article  |  2019  |  Stem Cells International

Bio-engineering a tissue flap utilizing a porous scaffold incorporating a human induced pluripotent stem cell-derived endothelial cell capillary network connected to a vascular pedicle

Anne M Kong, Kiryu K Yap, Shiang Y Lim, Diego Marre, Alice Pebay, Yi-wen Gerrand, Jarmon G Lees, Jason A Palmer, Wayne A Morrison, Geraldine M Mitchell

Journal article  |  2019  |  Acta Biomaterialia

Tissue flaps are used to cover large/poorly healing wounds, but involve complex surgery and donor site morbidity. In this study a ..

A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Lyujie Fang, Sandy SC Hung, Jennifer Yek, Layal El Wazan, Nguyen Tu, Shahnaz Khan, Shiang Y Lim, Alex W Hewitt, Raymond CB Wong

Journal article  |  2019  |  Molecular Therapy - Nucleic Acids

Methods for Assessing Scaffold Vascularization In Vivo

Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M Mitchell, Shiang Y Lim, Guei-Sheung Liu

Journal article  |  2019  |  Methods in molecular biology (Clifton, N.J.)

The success of tissue engineering hinges on the rapid and sufficient vascularization of the neotissue. For efficient vascular netw..

Mitochondrial Assays Using Cardiac Stem Cells.

Ayeshah A Rosdah, Lea MD Delbridge, Shiang Y Lim

Book Chapter  |  2019  |  Methods in Molecular Biology

Ischemic heart disease is the leading cause of death worldwide. Stem cell therapy to repair and regenerate the infarcted myocardiu..

Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?

Alison T Merryweather-Clarke, David Cook, Barbara Joo Lara, Peng Hua, Emmanouela Repapi, Neil Ashley, Shiang Y Lim, Suzanne M Watt

Journal article  |  2018  |  Stem Cell Research & Therapy

Role of lysophosphatidic acid in the retinal pigment epithelium and photoreceptors

Grace E Lidgerwood, Andrew J Morris, Alison Conquest, Maciej Daniszewski, Louise A Rooney, Shiang Y Lim, Damian Hernandez, Helena H Liang, Penelope Allen, Paul P Connell, Robyn H Guymer, Alex W Hewitt, Alice Pebay

Journal article  |  2018  |  Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells

Ashfaqul Hogue, Priyadharshini Sivakumaran, Simon T Bond, Naomi XY Ling, Anne M Kong, John W Scott, Nadeeka Bandara, Damian Hernandez, Guei-Sheung Liu, Raymond CB Wong, Michael T Ryan, Derek J Hausenloy, Bruce E Kemp, Jonathan S Oakhill, Brian G Drew, Alice Pebay, Shiang Y Lim

Journal article  |  2018  |  Cell Death Discovery

Biologically active constituents of the secretome of human W8B2( ) cardiac stem cells

Shuai Nie, Xin Wang, Priyadharshini Sivakumaran, Mark MW Chong, Xin Liu, Tara Karnezis, Nadeeka Bandara, Kaloyan Takov, Cameron J Nowell, Stephen Wilcox, Mitch Shambrook, Andrew F Hill, Nicole C Harris, Andrew E Newcomb, Padraig Strappe, Ramin Shayan, Damian Hernandez, Jordan Clarke, Eric Hanssen, Sean M Davidson, et al.

Journal article  |  2018  |  SCIENTIFIC REPORTS

NITRIC OXIDE DIRECTED REPROGRAMMING OF RAT BONE MARROW DERIVED MESENCHYMAL STEM CELLS INTO ENDOTHELIAL-LIKE CELLS VIA ACTIVATION OF WNT/B-CATENIN SIGNALING

Nadeeka Bandara, Shiang Y Lim, Le-Xin Wang, Padraig Strappe

Conference Proceedings  |  2018  |  JOURNAL OF GENE MEDICINE

Influence of continuous electrical stimulation on development of human cardiomyocytes from induced pluripotent stem cells

Damián Hernández, Rodney Millard, Priyadharshini Sivakumaran, A Kong, G Mitchell, Alice Pebay, R Sheperd, Gregory Dusting, S Lim

Journal article  |  2018  |  Conditioning Medicine

Regeneration of cardiac tissue remains an ideal approach to restore cardiac function after myocardial infarction. The ability of h..

Mdivi-1 Protects Human W8B2( ) Cardiac Stem Cells from Oxidative Stress and Simulated Ischemia-Reperfusion Injury

Ayeshah A Rosdah, Simon T Bond, Priyadharshini Sivakumaran, Ashfaqul Hoque, Jonathan S Oakhill, Brian G Drew, Lea MD Delbridge, Shiang Y Lim

Journal article  |  2017  |  Stem Cells and Development

Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency

Duncan E Crombie, Claire L Curl, Antonia JA Raaijmakers, Priyadharshini Sivakumaran, Tejal Kulkarni, Raymond CB Wong, Itsunari Minami, Marguerite V Evans-Galea, Shiang Y Lim, Lea Delbridge, Louise A Corben, Mirella Dottori, Norio Nakatsuji, Ian A Trounce, Alex W Hewitt, Martin B Delatycki, Martin F Pera, Alice Pebay

Journal article  |  2017  |  AGING-US

Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy

Raymond CB Wong, Shiang Y Lim, Sandy SC Hung, Stacey Jackson, Shahnaz Khan, Nicole J Van Bergen, Elisabeth De Smit, Helena H Liang, Lisa S Kearns, Linda Clarke, David A Mackey, Alex W Hewitt, Ian A Trounce, Alice Pebay

Journal article  |  2017  |  AGING-US

Molecular control of nitric oxide synthesis through eNOS and caveolin-1 interaction regulates osteogenic differentiation of adipose-derived stem cells by modulation of Wnt/beta-catenin signaling

Nadeeka Bandara, Saliya Gurusinghe, Shiang Yong Lim, Haying Chen, Shuangfeng Chen, Dawei Wang, Bryan Hilbert, Le-Xin Wang, Padraig Strappe

Journal article  |  2016  |  Stem Cell Research & Therapy

Harnessing the secretome of cardiac stem cells as therapy for ischemic heart disease

Ramin Khanabdali, Ayeshah A Rosdah, Gregory J Dusting, Shiang Y Lim

Journal article  |  2016  |  Biochemical Pharmacology

Mitochondrial fission - a drug target for cytoprotection or cytodestruction?

Ayeshah A Rosdah, Jessica K Holien, Lea MD Delbridge, Gregory J Dusting, Shiang Y Lim

Journal article  |  2016  |  Pharmacology Research & Perspectives

Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death

Shaun S Tan, Xiaolian Han, Priyadharshini Sivakumaran, Shiang Y Lim, Wayne A Morrison

Journal article  |  2016  |  Archives of Plastic Surgery

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Weiqing Zhan, Diego Marre, Geraldine M Mitchell, Wayne A Morrison, Shiang Y Lim

Journal article  |  2016  |  Journal of Visualized Experiments

Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells

Sandy SC Hung, Nicole J Van Bergen, Stacey Jackson, Helena Liang, David A Mackey, Damian Hernandez, Shiang Y Lim, Alex W Hewitt, Ian Trounce, Alice Pebay, Raymond CB Wong

Journal article  |  2016  |  AGING-US

Minicircle DNA-mediated endothelial nitric oxide synthase gene transfer enhances angiogenic responses of bone marrow-derived mesenchymal stem cells

Nadeeka Bandara, Saliya Gurusinghe, Haiying Chen, Shuangfeng Chen, Le-xin Wang, Shiang Y Lim, Padraig Strappe

Journal article  |  2016  |  Stem Cell Research & Therapy

Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Grace E Lidgerwood, Shiang Y Lim, Duncan E Crombie, Ray Ali, Katherine P Gill, Damian Hernandez, Josh Kie, Alison Conquest, Hayley S Waugh, Raymond CB Wong, Helena H Liang, Alex W Hewitt, Kathryn C Davidson, Alice Pebay

Journal article  |  2016  |  STEM CELL REVIEWS AND REPORTS

Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications

Elsa C Chan, Shyh-Ming Kuo, Anne M Kong, Wayne A Morrison, Gregory J Dusting, Geraldine M Mitchell, Shiang Y Lim, Guei-Sheung Liu

Journal article  |  2016  |  PLOS ONE

Enhancing Human Cardiomyocyte Differentiation from Induced Pluripotent Stem Cells with Trichostatin A.

Shiang Y Lim, Priyadharshini Sivakumaran, Duncan E Crombie, Gregory J Dusting, Alice Pébay, Rodney J Dilley

Journal article  |  2016  |  Methods in molecular biology (Clifton, N.J.)

Human induced pluripotent stem (iPS) cells are a promising source of autologous cardiomyocytes to repair and regenerate myocardium..

Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Damian Hernandez, Rodney Millard, Priyadharshini Sivakumaran, Raymond CB Wong, Duncan E Crombie, Alex W Hewitt, Helena Liang, Sandy SC Hung, Alice Pebay, Robert K Shepherd, Gregory J Dusting, Shiang Y Lim

Journal article  |  2016  |  STEM CELLS INTERNATIONAL

Cardiac Repair With a Novel Population of Mesenchymal Stem Cells Resident in the Human Heart

Yuan Zhang, Priyadharshini Sivakumaran, Andrew E Newcomb, Damian Hernandez, Nicole Harris, Ramin Khanabdali, Guei-Sheung Liu, Darren J Kelly, Alice Pebay, Alex W Hewitt, Andrew Boyle, Richard Harvey, Wayne A Morrison, David A Elliott, Gregory J Dusting, Shiang Y Lim

Journal article  |  2015  |  STEM CELLS

Efficient Generation of Integration-Free Human Induced Pluripotent Stem Cells From Keratinocytes by Simple Transfection of Episomal Vectors

Yulan Piao, Sandy Shen-Chi Hung, Shiang Y Lim, Raymond Ching-Bong Wong, Minoru SH Ko

Journal article  |  2014  |  STEM CELLS Translational Medicine

Ischemic preconditioning for cell-based therapy and tissue engineering

Sarah T Hsiao, Rodney J Dilley, Gregory J Dusting, Shiang Y Lim

Journal article  |  2014  |  Pharmacology and Therapeutics

NADPH oxidase in tissue repair and regeneration

EC Chan, GS Liu, CL Roulston, SY Lim, GJ Dusting

Book Chapter  |  2014

Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering

Shiang Y Lim, Priyadharshini Sivakumaran, Duncan E Crombie, Gregory J Dusting, Alice Pebay, Rodney J Dilley

Journal article  |  2013  |  STEM CELLS TRANSLATIONAL MEDICINE

Growing Vascularized Heart Tissue From Stem Cells

Shiang Y Lim, Damian Hernandez, Gregory J Dusting

Journal article  |  2013  |  Journal of Cardiovascular Pharmacology

Hypoxic Conditioning Enhances the Angiogenic Paracrine Activity of Human Adipose-Derived Stem Cells

Sarah T Hsiao, Zerina Lokmic, Hitesh Peshavariya, Keren M Abberton, Gregory J Dusting, Shiang Y Lim, Rodney J Dilley

Journal article  |  2013  |  Stem Cells and Development

Nox4 modulates collagen production stimulated by transforming growth factor beta 1 in vivo and in vitro

Elsa C Chan, Hitesh M Peshavariya, Guei-Sheung Liu, Fan Jiang, Shiang-Yong Lim, Gregory J Dusting

Journal article  |  2013  |  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Enrichment of neonatal rat cardiomyocytes in primary culture facilitates long-term maintenance of contractility in vitro

Phong D Nguyen, Sarah T Hsiao, Priyadharshini Sivakumaran, Shiang Y Lim, Rodney J Dilley

Journal article  |  2012  |  AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Ischemic Preconditioning Promotes Intrinsic Vascularization and Enhances Survival of Implanted Cells in an In Vivo Tissue Engineering Model

Shiang Y Lim, Sarah T Hsiao, Zerina Lokmic, Priyadharshini Sivakumaran, Gregory J Dusting, Rodney J Dilley

Journal article  |  2012  |  Tissue Engineering. Part A. Tissue Engineering

Comparative Analysis of Paracrine Factor Expression in Human Adult Mesenchymal Stem Cells Derived from Bone Marrow, Adipose, and Dermal Tissue

Sarah Tzu-Feng Hsiao, Azar Asgari, Zerina Lokmic, Rodney Sinclair, Gregory James Dusting, Shiang Yong Lim, Rodney James Dilley

Journal article  |  2012  |  Stem Cells and Development

Hypoxic Preconditioning Enhances Survival of Human Adipose-Derived Stem Cells and Conditions Endothelial Cells In Vitro

Samantha Licy Stubbs, Sarah Tzu-Feng Hsiao, Hitesh Mahendrabhai Peshavariya, Shiang Yong Lim, Gregory James Dusting, Rodney James Dilley

Journal article  |  2012  |  STEM CELLS AND DEVELOPMENT

In vivo tissue engineering chamber supports human induced pluripotent stem cell survival and rapid differentiation

Shiang Y Lim, Dcing Geun Lee, Priyadharshini Sivakumaran, Duncan Crombie, John Slavin, Mirella Dottori, Brock Conley, Mark Denham, Jessie Leung, Richard Tee, Gregory J Dusting, Alice Pebay, Rodney J Dilley

Journal article  |  2012  |  Biochemical and Biophysical Research Communications

Remote ischennic conditioning: from bench to bedside

Shiang Yong Lim, Derek John Hausenloy

Journal article  |  2012  |  Frontiers in Physiology

Mitochondrial cyclophilin-D as a potential therapeutic target for post-myocardial infarction heart failure.

Shiang Y Lim, Derek J Hausenloy, Sapna Arjun, Anthony N Price, Sean M Davidson, Mark F Lythgoe, Derek M Yellon

Journal article  |  2011  |  Journal of Cellular and Molecular Medicine

The pharmacological inhibition or genetic ablation of cyclophilin-D (CypD), a critical regulator of the mitochondrial permeability..

Rapid assessment of myocardial infarct size in rodents using multi-slice inversion recovery late gadolinium enhancement CMR at 9.4T.

Anthony N Price, King K Cheung, Shiang Y Lim, Derek M Yellon, Derek J Hausenloy, Mark F Lythgoe

Journal article  |  2011  |  Journal of Cardiovascular Magnetic Resonance

BACKGROUND: Myocardial infarction (MI) can be readily assessed using late gadolinium enhancement (LGE) cardiovascular magnetic res..

Failure of the adipocytokine, resistin, to protect the heart from ischemia-reperfusion injury.

Christopher CT Smith, Shiang Y Lim, Abigail M Wynne, Vivek Sivaraman, Sean M Davidson, Mihaela M Mocanu, Derek J Hausenloy, Derek M Yellon

Journal article  |  2011  |  Journal of Cardiovascular Pharmacology and Therapeutics

Experimental studies have linked the adipocytokines with acute cardioprotection. Whether the adipocytokine, resistin, confers prot..

Cytoprotection and Preconditioning for Stem Cell Therapy

SY Lim, RJ Dilley, GJ Dusting

Book Chapter  |  2011

Mitochondrial cyclophilin-D as a critical mediator of ischaemic preconditioning.

Derek J Hausenloy, Shiang Y Lim, Sang-Ging Ong, Sean M Davidson, Derek M Yellon

Journal article  |  2010  |  Cardiovascular Research

AIMS: It has been suggested that mitochondrial reactive oxygen species (ROS), Akt and Erk1/2 and more recently the mitochondrial p..

Mitochondrial cyclophilin-D as a critical mediator of ischaemic preconditioning

DJ Hausenloy, SY Lim, SG Ong, SM Davidson, DM Yellon

Journal article  |  2010  |  Cardiovascular Research

The neural and humoral pathways in remote limb ischemic preconditioning.

Shiang Y Lim, Derek M Yellon, Derek J Hausenloy

Journal article  |  2010  |  Basic Research in Cardiology

Remote ischaemic preconditioning (RIPC) is a therapeutic intervention that has been demonstrated to reduce myocardial injury in th..

Absence of the inhibitory G-protein Galphai2 predisposes to ventricular cardiac arrhythmia.

Zia Zuberi, Muriel Nobles, Sonia Sebastian, Alex Dyson, Shiang Y Lim, Ross Breckenridge, Lutz Birnbaumer, Andrew Tinker

Journal article  |  2010  |  Circulation: Arrhythmia and Electrophysiology

BACKGROUND: We explored the role that inhibitory heterotrimeric G-proteins play in ventricular arrhythmia. METHODS AND RESULTS: Mi..

Inhibiting mitochondrial fission protects the heart against ischemia/reperfusion injury.

Sang-Bing Ong, Sapna Subrayan, Shiang Y Lim, Derek M Yellon, Sean M Davidson, Derek J Hausenloy

Journal article  |  2010  |  Circulation

BACKGROUND: Whether alterations in mitochondrial morphology affect the susceptibility of the heart to ischemia/reperfusion injury ..

Activation of protease activated receptor-2 induces delayed cardioprotection in anesthetized mice.

Shiang Y Lim, Cherry L Wainwright, Simon Kennedy, Kathleen A Kane

Journal article  |  2009  |  Cardiovascular Drugs and Therapy

The cannabinoid CB1 receptor antagonist, rimonabant, protects against acute myocardial infarction.

Shiang Y Lim, Sean M Davidson, Derek M Yellon, Christopher CT Smith

Journal article  |  2009  |  Basic Research in Cardiology

CB1 antagonism is associated with reduced doxorubicin-induced cardiotoxicity and decreased cerebrocortical infarction. Rimonabant,..

The novel adipocytokine visfatin exerts direct cardioprotective effects.

Shiang Y Lim, Sean M Davidson, Ajeev J Paramanathan, Christopher CT Smith, Derek M Yellon, Derek J Hausenloy

Journal article  |  2008  |  Journal of Cellular and Molecular Medicine

Visfatin is an adipocytokine capable of mimicking the glucose-lowering effects of insulin and activating the pro-survival kinases ..

The cardioprotective effect of necrostatin requires the cyclophilin-D component of the mitochondrial permeability transition pore.

SY Lim, SM Davidson, MM Mocanu, DM Yellon, CCT Smith

Journal article  |  2007  |  Cardiovascular Drugs and Therapy

BACKGROUND: Necrostatin (Nec-1) protects against ischemia-reperfusion (IR) injury in both brain and heart. We have previously repo..

Apelin-13 and apelin-36 exhibit direct cardioprotective activity against ischemia-reperfusion injury.

James C Simpkin, Derek M Yellon, Sean M Davidson, Shiang Y Lim, Abigail M Wynne, Christopher CT Smith

Journal article  |  2007  |  Basic Research in Cardiology

Protection against myocardial ischemia-reperfusion (I/R) injury involves activation of phosphatidylinositol-3-OH kinase (PI3K)- Ak..

Necrostatin: a potentially novel cardioprotective agent?

Christopher CT Smith, Sean M Davidson, Shiang Y Lim, James C Simpkin, John S Hothersall, Derek M Yellon

Journal article  |  2007  |  Cardiovascular Drugs and Therapy

BACKGROUND: Necrostatin-1 (Nec-1), a small tryptophan-based molecule, was recently reported to protect the cerebral cortex against..

Preconditioning and postconditioning: the essential role of the mitochondrial permeability transition pore.

Shiang Y Lim, Sean M Davidson, Derek J Hausenloy, Derek M Yellon

Journal article  |  2007  |  Cardiovascular Research

OBJECTIVE: The opening of the mitochondrial permeability transition pore (mPTP) at the time of myocardial reperfusion is a critica..

Activation of mouse protease-activated receptor-2 induces lymphocyte adhesion and generation of reactive oxygen species.

SY Lim, GM Tennant, S Kennedy, CL Wainwright, KA Kane

Journal article  |  2006  |  British Journal of Pharmacology

BACKGROUND AND PURPOSE: Protease-activated receptor-2 (PAR-2) is expressed on lymphocytes and endothelial cells, and plays a signi..