I have interest and expertise in using next generation sequencing for the detection, classification, and investigation of animal pathogens. I'm broadly interested in the prevalence and the genomics of bacterial and viral diseases in wildlife, including pathogens with zoonotic potential.
I'm also interested in using next generation tools such as Nanopore sequencing for surveillance and discovery of potential pathogens in wildlife and livestock.
I was born and raised in Melbourne, Australia, and have been at the University of Melbourne as a student or staff member since 2005. I undertook a Bachelor of Science in Zoology and Microbiology (2005 - 2008), followed by a Master of Science in Zoology (2009-2010), in collaboration with the Victorian Infectious Disease Reference Laboratory. My Masters project focused on Mycobacterium ulcerans in common brushtail and ringtail possums. I then moved to the Faculty of Veterinary and Agricultural Sciences and worked for three years as a research assistant testing a vaccine candidate for ILTV (2010-2014). After this time I undertook a PhD investigating Chlamydia and retrovirus in koalas (2014-2017) using molecular detection and next generation sequencing to compare infections in different geographical regions of the country.
At the end of 2017, I began a post-doctoral research project, piloting the use of nanopore sequencing as a tool for pathogen surveillance in Victorian wild birds. I am also actively involved with, or have worked on, using next generation sequencing to investigate:
- Using long and short read sequencing technologies to investigate ILTV recombination
- Genomics of Mycoplasma bovis in cattle- Metagenomic investigations into equine reproductive losses- Metagenomic investigations into Australian arboreal marsupials
- Bacterial causes of bovine digital dermatitis
Find me on other websites
Asia Pacific Centre for Animal Health
Personal website: alegione.github.io
Google Scholar: scholar.google.com.au/citations?user=OmIonF8AAAAJ
Twitter.com/alegione (views are my own and not of the University of Melbourne)
Find out more about Alistair Legione's experience
Alistair Legione's highlights
Alistair Legione's selected work
Detection of Coxiella burnetii and equine herpesvirus 1, but not Leptospira spp. or Toxopl..
Differential Response of the Chicken Trachea to Chronic Infection with Virulent Mycoplasma..
Displaying the 5 most recent projects by Alistair Legione.
Displaying the 28 most recent scholarly works by Alistair Legione.
Development of a rapid technique for extraction of viral DNA/RNA for whole genome sequencing directly from clinical liver tissues
Kinza Asif, Denise O'Rourke, Alistair R Legione, Penelope A Steer-Cope, Pollob Shil, Marc S Marenda, Amir H Noormohammadi
Journal article | 2020 | Journal of Virological Methods
Characterisation of the entire genome of Fowl aviadenoviruses (FAdV) requires isolation and propagation of the virus in chicken em..
Detection of Coxiella burnetii and equine herpesvirus 1, but not Leptospira spp. or Toxoplasma gondii, in cases of equine abortion in Australia - a 25 year retrospective study
Rumana Akter, Alistair Legione, Fiona M Sansom, Charles M El-Hage, Carol A Hartley, James R Gilkerson, Joanne M Devlin
Journal article | 2020 | PLOS ONE
Equine abortion is a cause of severe economic loss to the equine industry. Equine herpesvirus 1 is considered a primary cause of i..
Differential Response of the Chicken Trachea to Chronic Infection with Virulent Mycoplasma gallisepticum Strain Ap3AS and Vaxsafe MG (Strain ts-304): a Transcriptional Profile
Sathya N Kulappu Arachchige, Neil D Young, Pollob K Shil, Alistair R Legione, Anna Kanci Condello, Glenn F Browning, Nadeeka K Wawegama
Journal article | 2020 | Infection and Immunity
Mycoplasma gallisepticum is the primary etiological agent of chronic respiratory disease in chickens. Live attenuated vaccines are..
SURVEILLANCE FOR CHLAMYDIA SPP. WITH MULTILOCUS SEQUENCE TYPING ANALYSIS IN WILD AND CAPTIVE BIRDS IN VICTORIA, AUSTRALIA
Jemima Amery-Gale, Alistair R Legione, Marc S Marenda, Jane Owens, Paul A Eden, Barbara M Konsak-Ilievski, Pam L Whiteley, Elizabeth C Dobson, Elizabeth A Browne, Ron F Slocombe, Joanne M Devlin
Journal article | 2020 | Journal of Wildlife Diseases
Chlamydia psittaci typically infects birds and can cause outbreaks of avian chlamydiosis, but it also has the potential to cause z..
Bovine digital dermatitis in Victoria, Australia.
J Hesseling, AR Legione, MA Stevenson, CI McCowan, MF Pyman, C Finochio, D Nguyen, CL Roic, OL Thiris, AJ Zhang, G van Schaik, JE Coombe
Journal article | 2019 | Australian Veterinary Journal
AIMS: The objectives of this study were to estimate the prevalence of digital dermatitis (DD) in Victoria, Australia, and to inves..
Disease surveillance in wild Victorian cacatuids reveals co-infection with multiple agents and detection of novel avian viruses
Michelle Sutherland, Subir Sarker, Paola K Vaz, Alistair R Legione, Joanne M Devlin, Patricia L Macwhirter, Pamela L Whiteley, Shane R Raidal
Journal article | 2019 | Veterinary Microbiology
Polychromophilus melanipherus and haemoplasma infections not associated with clinical signs in southern bent-winged bats (Miniopterus orianae bassanii) and eastern bent-winged bats (Miniopterus orianae oceanensis)
Peter H Holz, Linda F Lumsden, Alistair R Legione, Jasmin Hufschmid
Journal article | 2019 | International Journal for Parasitology: Parasites and Wildlife
Detection and Differentiation of Two Koala Gammaherpesviruses Using High Resolution Melt (Hrm) Analysis Reveals Differences in Viral Prevalence and Clinical Associations in a Large Study of Free-Ranging Koalas
PK Vaz, AR Legione, CA Hartley, JM Devlin
Journal article | 2019 | Journal of Clinical Microbiology
The iconic koala (Phascolarctos cinereus) is host to two divergent gammaherpesviruses, phascolarctid gammaherpesviruses 1 and 2 (P..
A Novel Glaesserella sp Isolated from Pigs with Severe Respiratory Infections Has a Mosaic Genome with Virulence Factors Putatively Acquired by Horizontal Transfer
Anne E Watt, Glenn F Browning, Alistair R Legione, Rhys N Bushell, Andrew Stent, Ross S Cutler, Neil D Young, Marc S Marenda
Journal article | 2018 | Applied and Environmental Microbiology
Virus survey in populations of two subspecies of bent-winged bats (Miniopterus orianae bassanii and oceanensis) in south-eastern Australia reveals a high prevalence of diverse herpesviruses
Peter H Holz, Linda F Lumsden, Julian Druce, Alistair R Legione, Paola Vaz, Joanne M Devlin, Jasmin Hufschmid
Journal article | 2018 | PLOS ONE
Infectious Laryngotracheitis Virus Viral Chemokine-Binding Protein Glycoprotein G Alters Transcription of Key Inflammatory Mediators In Vitro and In Vivo
Mauricio JC Coppo, Joanne M Devlin, Alistair R Legione, Paola K Vaz, Sang-Won Lee, Jose A Quinteros, James R Gilkerson, Nino Ficorilli, Patrick C Reading, Amir H Noormohammadi, Carol A Hartley
Journal article | 2018 | J Virol
Koala retrovirus genotyping analyses reveal a low prevalence of KoRV-A in Victorian koalas and an association with clinical disease
Alistair R Legione, Jade LS Patterson, Pam Whiteley, Simon M Firestone, Megan Curnick, Kate Bodley, Michael Lynch, James R Gilkerson, Fiona M Sansom, Joanne M Devlin
Journal article | 2017 | Journal of Medical Microbiology
Full Genome Analysis of Australian Infectious Bronchitis Viruses Suggests Frequent Recombination Events between Vaccine Strains and Multiple Phylogenetically Distant Avian Coronaviruses of Unknown Origin
JA Quinteros, SW Lee, PF Markham, AH Noormohammadi, CA Hartley, AR Legione, MJC Coppo, PK Vaz, GF Browning
Journal article | 2016 | Veterinary Microbiology
Australian strains of infectious bronchitis virus (IBV) have been evolving independently for many years, with control achieved by ..
Identification of unusual Chlamydia pecorum genotypes in Victorian koalas (Phascolarctos cinereus) and clinical variables associated with infection
Alistair R Legione, Jade LS Patterson, Pam L Whiteley, Jemima Amery-Gale, Michael Lynch, Leesa Haynes, James R Gilkerson, Adam Polkinghorne, Joanne M Devlin, Fiona M Sansom
Journal article | 2016 | Journal of Medical Microbiology
Protection Induced in Broiler Chickens following Drinking-Water Delivery of Live Infectious Laryngotracheitis Vaccines against Subsequent Challenge with Recombinant Field Virus
Mesula G Korsa, Glenn F Browning, Mauricio JC Coppo, Alistair R Legione, James R Gilkerson, Amir H Noormohammadi, Paola K Vaz, Sang-Won Lee, Joanne M Devlin, Carol A Hartley
Journal article | 2015 | PLOS ONE
Analysis of the complete genomic sequences of two virus subpopulations of the Australian infectious bronchitis virus vaccine VicS
Jose A Quinteros, Philip F Markham, Sang-Won Lee, Kylie A Hewson, Carol A Hartley, Alistair R Legione, Mauricio JC Coppo, Paola K Vaz, Glenn F Browning
Journal article | 2015 | AVIAN PATHOLOGY
THE PREVALENCE AND CLINICAL SIGNIFICANCE OF CHLAMYDIA INFECTION IN ISLAND AND MAINLAND POPULATIONS OF VICTORIAN KOALAS (PHASCOLARCTOS CINEREUS)
Jade LS Patterson, Michael Lynch, Garry A Anderson, Amir H Noormohammadi, Alistair Legione, James R Gilkerson, Joanne M Devlin
Journal article | 2015 | Journal of Wildlife Diseases
Growth Kinetics and Transmission Potential of Existing and Emerging Field Strains of Infectious Laryngotracheitis Virus
Sang-Won Lee, Carol A Hartley, Mauricio JC Coppo, Paola K Vaz, Alistair R Legione, Jose A Quinteros, Amir H Noormohammadi, Phillip F Markham, Glenn F Browning, Joanne M Devlin
Journal article | 2015 | PLOS ONE
Development and Validation of TaqMan Real-Time Polymerase Chain Reaction Assays for the Quantitative and Differential Detection of Wild-Type Infectious Laryngotracheitis Viruses from a Glycoprotein G–Deficient Candidate Vaccine Strain
Niraj K Shil, Alistair R Legione, Phillip F Markham, Amir H Noormohammadi, Joanne M Devlin
Journal article | 2015 | Avian Diseases
Infectious laryngotracheitis (ILT) is a significant upper respiratory tract disease of chickens with a worldwide distribution. Dif..
Cross-Protective Immune Responses Between Genotypically Distinct Lineages of Infectious Laryngotracheitis Viruses
Sang-Won Lee, Philip F Markham, Mauricio JC Coppo, Alistair R Legione, Niraj K Shil, Jose A Quinteros, Amir H Noormohammadi, Glenn F Browning, Carol A Hartley, Joanne M Devlin
Journal article | 2014 | Avian Diseases
Clinical, Microbiological and Pathological Findings of Mycobacterium ulcerans Infection in Three Australian Possum Species
Carolyn R O'Brien, Kathrine A Handasyde, Jennifer Hibble, Caroline J Lavender, Alistair R Legione, Christina McCowan, Maria Globan, Anthony T Mitchell, Helen E McCracken, Paul DR Johnson, Janet AM Fyfe
Journal article | 2014 | PLoS Neglected Tropical Diseases
Safety and vaccine efficacy of a glycoprotein G deficient strain of infectious laryngotracheitis virus delivered in ovo
Alistair R Legione, Mauricio JC Coppo, Sang-Won Lee, Amir H Noormohammadi, Carol A Hartley, Glenn F Browning, James R Gilkerson, Denise O'Rourke, Joanne M Devlin
Journal article | 2012 | Vaccine
A Major Role for Mammals in the Ecology of Mycobacterium ulcerans
Janet AM Fyfe, Caroline J Lavender, Kathrine A Handasyde, Alistair R Legione, Carolyn R O'Brien, Timothy P Stinear, Sacha J Pidot, Torsten Seemann, M Eric Benbow, John R Wallace, Christina McCowan, Paul DR Johnson
Journal article | 2010 | PLOS NEGLECTED TROPICAL DISEASES
Honours, Awards and Fellowships
Lecturer (Veterinary Microbiology & Public Health)
The Australian Society for Microbiology
Wildlife Diseases Association
Wildlife Diseases Association - Australasian Section
PhD (Veterinary Science)
University of Melbourne
Doctor of Philosophy
The University of Melbourne
Specialist Certificate in Teaching for Graduate Researchers
University of Melbourne
Master of Science (Zoology)
University of Melbourne
Bachelor of Science
University of Melbourne