Melinda Hardy

A Sensitive Whole Blood Assay Detects Antigen-Stimulated Cytokine Release From CD4 T Cells and Facilitates Immunomonitoring in a Phase 2 Clinical Trial of Nexvax2 in Coeliac Disease

Melinda Y Hardy, Gautam Goel, Amy K Russell, Swee Lin G Chen Yi Mei, Gregor JE Brown, Suyue Wang, Evan Szymczak, Ruan Zhang, Kaela E Goldstein, Kristin M Neff, Leslie J Williams, Kenneth E Truitt, John L Dzuris, Jason A Tye-Din, Robert P Anderson

Journal article  |  2021  |  FRONTIERS IN IMMUNOLOGY

Improved blood tests assessing the functional status of rare gluten-specific CD4+ T cells are needed to effectively monitor experi..

Whole blood interleukin-2 release test to detect and characterize rare circulating gluten-specific T cell responses in coeliac disease

RP Anderson, G Goel, MY Hardy, AR Russell, S Wang, E Szymczak, R Zhang, KE Goldstein, M Neff, KE Truitt, LJ Williams, JL Dzuris, JA Tye-Din

Journal article  |  2021  |  CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Whole blood cytokine release assays (CRA) assessing cellular immunity to gluten could simplify the diagnosis and monitoring of coe..

Characterisation of clinical and immune reactivity to barley and rye ingestion in children with coeliac disease

Melinda Y Hardy, Amy K Russell, Catherine Pizzey, Claerwen M Jones, Katherine A Watson, Nicole L La Gruta, Donald J Cameron, Jason A Tye-Din

Journal article  |  2020  |  GUT

OBJECTIVE: Barley and rye are major components of the Western diet, and historic feeding studies indicate that they cause clinical..

Circulating gluten-specific, but not CMV-specific, CD39( ) regulatory T cells have an oligoclonal TCR repertoire

Laura Cook, C Mee Ling Munier, Nabila Seddiki, Melinda Y Hardy, Robert P Anderson, John Zaunders, Jason A Tye-Din, Anthony D Kelleher, David van Bockel

Journal article  |  2020  |  CLINICAL & TRANSLATIONAL IMMUNOLOGY

Objectives: Understanding the T cell receptor (TCR) repertoire of regulatory CD4+ T-cell (Treg) populations is important for strat..

T cell receptor cross-reactivity between gliadin and bacterial peptides in celiac disease

Jan Petersen, Laura Ciacchi, Mai T Tran, Khai Lee Loh, Yvonne Kooy-Winkelaar, Nathan P Croft, Melinda Y Hardy, Zhenjun Chen, James McCluskey, Robert P Anderson, Anthony W Purcell, Jason A Tye-Din, Frits Koning, Hugh H Reid, Jamie Rossjohn

Journal article  |  2019  |  NATURE STRUCTURAL & MOLECULAR BIOLOGY

The human leukocyte antigen (HLA) locus is strongly associated with T cell-mediated autoimmune disorders. HLA-DQ2.5-mediated celia..

Preparation and Characterization of Avenin-Enriched Oat Protein by Chill Precipitation for Feeding Trials in Celiac Disease.

Greg Tanner, Angéla Juhász, Christakis George Florides, Mitchell Nye-Wood, Frank Békés, Michelle L Colgrave, Amy K Russell, Melinda Y Hardy, Jason A Tye-Din

Journal article  |  2019  |  Frontiers in Nutrition

The safety of oats for people with celiac disease remains unresolved. While oats have attractive nutritional properties that can i..

T cells in coeliac disease: a rational target for diagnosis and therapy

Melinda Y Hardy, Jason A Tye-Din

Journal article  |  2018  |  NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY

New findings show that disease-specific T cells that target gluten in patients with coeliac disease persist for decades. The data ..

Resolving incomplete single nucleotide polymorphism tagging of HLA-DQ2.2 for coeliac disease genotyping using digital droplet PCR

MY Hardy, N Ontiveros, MD Varney, JA Tye-Din

Journal article  |  2018  |  HLA

A hallmark of coeliac disease (CD) is the exceptionally strong genetic association with HLA-DQ2.5, DQ8, and DQ2.2. HLA typing prov..

Circulating gluten-specific FOXP3( )CD39( ) regulatory T cells have impaired suppressive function in patients with celiac disease

Laura Cook, C Mee Ling Munier, Nabila Seddiki, David van Bockel, Noe Ontiveros, Melinda Y Hardy, Jana K Gillies, Megan K Levings, Hugh H Reid, Jan Petersen, Jamie Rossjohn, Robert P Anderson, John J Zaunders, Jason A Tye-Din, Anthony D Kelleher

Journal article  |  2017  |  JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

BACKGROUND: Celiac disease is a chronic immune-mediated inflammatory disorder of the gut triggered by dietary gluten. Although the..

Coeliac disease: a unique model for investigating broken tolerance in autoimmunity

Melinda Y Hardy, Jason A Tye-Din

Journal article  |  2016  |  CLINICAL & TRANSLATIONAL IMMUNOLOGY

Coeliac disease, a prevalent immune-mediated enteropathy driven by dietary gluten, provides an exceptional human model to dissect ..