Alessandro Uboldi

Transcriptional modification of host cells harboring Toxoplasma gondii bradyzoites prevents IFN gamma-mediated cell death

Simona Seizova, Ushma Ruparel, Alexandra L Garnham, Stefanie M Bader, Alessandro D Uboldi, Michael J Coffey, Lachlan W Whitehead, Kelly L Rogers, Christopher J Tonkin

Journal article  |  2022  |  CELL HOST & MICROBE

Toxoplasma gondii develops a latent infection in the muscle and central nervous system that acts as a reservoir for acute-stage re..

Pre-existing Toxoplasma gondii infection increases susceptibility to pentylenetetrazol-induced seizures independent of traumatic brain injury in mice.

Tamara L Baker, Alessandro D Uboldi, Christopher J Tonkin, David K Wright, Anh Vo, Trevor Wilson, Richelle Mychasiuk, Stuart J McDonald, Bridgette D Semple, Mujun Sun, Sandy R Shultz

Journal article  |  2022  |  Front Mol Neurosci

INTRODUCTION: Post-traumatic epilepsy (PTE) is a debilitating chronic outcome of traumatic brain injury (TBI), and neuroinflammati..

Depletion of a Toxoplasma porin leads to defects in mitochondrial morphology and contacts with the endoplasmic reticulum

Natalia Mallo, Jana Ovciarikova, Erica S Martins-Duarte, Stephan C Baehr, Marco Biddau, Mary-Louise Wilde, Alessandro D Uboldi, Leandro Lemgruber, Christopher J Tonkin, Jeremy G Wideman, Clare R Harding, Lilach Sheiner

Journal article  |  2021  |  JOURNAL OF CELL SCIENCE

The voltage-dependent anion channel (VDAC) is a ubiquitous channel in the outer membrane of the mitochondrion with multiple roles ..

Environmental sensing and regulation of motility in Toxoplasma

Alessandro D Uboldi, Mary-Louise Wilde, Stefanie M Bader, Christopher J Tonkin

Journal article  |  2020  |  MOLECULAR MICROBIOLOGY

Toxoplasma and other apicomplexan parasites undergo a unique form of cellular locomotion referred to as "gliding motility." Glidin..

Metabolomic Analysis of Toxoplasma gondii Tachyzoites

Elizabeth FB King, Simon A Cobbold, Alessandro D Uboldi, Christopher J Tonkin, Malcolm J McConville

Journal article  |  2020  |  TOXOPLASMA GONDII: METHODS AND PROTOCOLS

This protocol describes the use of 13C-stable isotope labeling, combined with metabolite profiling, to investigate the metabolism ..

An apically located hybrid guanylate cyclase-ATPase is critical for the initiation of Ca2 signaling and motility in Toxoplasma gondii

Luning Yang, Alessandro D Uboldi, Simona Seizova, Mary-Louise Wilde, Michael J Coffey, Nicholas J Katris, Yoshiki Yamaryo-Botte, Martina Kocan, Ross AD Bathgate, Rebecca J Stewart, Malcolm J McConville, Philip E Thompson, Cyrille Y Botte, Christopher J Tonkin

Journal article  |  2019  |  JOURNAL OF BIOLOGICAL CHEMISTRY

Protozoan parasites of the phylum Apicomplexa actively move through tissue to initiate and perpetuate infection. The regulation of..

Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection

Sachin Khurana, Michael J Coffey, Alan John, Alessandro D Uboldi, My-Hang Huynh, Rebecca J Stewart, Vern B Carruthers, Christopher J Tonkin, Ethan D Goddard-Borger, Nichollas E Scott

Journal article  |  2019  |  JOURNAL OF BIOLOGICAL CHEMISTRY

Toxoplasma gondii is a ubiquitous, obligate intracellular eukaryotic parasite that causes congenital birth defects, disease in imm..

Protein kinase A negatively regulates Ca2 signalling in Toxoplasma gondii

Alessandro D Uboldi, Mary-Louise Wilde, Emi A McRae, Rebecca J Stewart, Laura F Dagley, Luning Yang, Nicholas J Katris, Sanduni Hapuarachchi, Michael J Coffey, Adele M Lehane, Cyrille Y Botte, Ross F Waller, Andrew Webb, Malcolm J McConville, Christopher J Tonkin

Journal article  |  2018  |  PLOS BIOLOGY

The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stag..

Development of a Novel CD4( ) TCR Transgenic Line That Reveals a Dominant Role for CD8( ) Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria

Daniel Fernandez-Ruiz, Lei Shong Lau, Nazanin Ghazanfari, Claerwen M Jones, Wei Yi Ng, Gayle M Davey, Dorothee Berthold, Lauren Holz, Yu Kato, Matthias H Enders, Ganchimeg Bayarsaikhan, Sanne H Hendriks, Lianne IM Lansink, Jessica A Engel, Megan SF Soon, Kylie R James, Anton Cozijnsen, Vanessa Mollard, Alessandro D Uboldi, Christopher J Tonkin, et al.

Journal article  |  2017  |  JOURNAL OF IMMUNOLOGY

We describe an MHC class II (I-Ab)-restricted TCR transgenic mouse line that produces CD4+ T cells specific for Plasmodium species..

A forward genetic screen identifies a negative regulator of rapid Ca2 -dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

James M McCoy, Rebecca J Stewart, Alessandro D Uboldi, Dongdi Li, Jan Schroder, Nicollas E Scott, Anthony T Papenfuss, Adele M Lehane, Leonard J Foster, Christopher J Tonkin

Journal article  |  2017  |  JOURNAL OF BIOLOGICAL CHEMISTRY

Toxoplasma gondii, like all apicomplexan parasites, uses Ca2+ signaling pathways to activate gliding motility to power tissue diss..