Shenggen Yao

Chemical Exchange of Hydroxyl Groups in Lipidic Cubic Phases Characterized by NMR

Thomas G Meikle, David W Keizer, Jeffrey J Babon, Calum J Drummond, Frances Separovic, Charlotte E Conn, Shenggen Yao

Journal article  |  2021  |  JOURNAL OF PHYSICAL CHEMISTRY B

Proton transportation in proximity to the lipid bilayer membrane surface, where chemical exchange represents a primary pathway, is..

Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR

Thomas G Meikle, David W Keizer, Jeffrey J Babon, Calum J Drummond, Frances Separovic, Charlotte E Conn, Shenggen Yao

Journal article  |  2020  |  LANGMUIR

Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found..

Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids

Thomas G Meikle, Ashish Sethi, David W Keizer, Jeffrey J Babon, Frances Separovic, Paul R Gooley, Charlotte E Conn, Shenggen Yao

Journal article  |  2019  |  JOURNAL OF MAGNETIC RESONANCE

Lipidic cubic phases, which form spontaneously via the self-assembly of certain lipids in an aqueous environment, are highly prosp..

Structural insights into BCL2 pro-survival protein interactions with the key autophagy regulator BECN1 following phosphorylation by STK4/MST1

Erinna F Lee, Nicholas A Smith, Tatiana P Soares da Costa, Nastaran Meftahi, Shenggen Yao, Tiffany J Harris, Sharon Tran, Anne Pettikiriarachchi, Matthew A Perugini, David W Keizer, Marco Evangelista, Brian J Smith, W Douglas Fairlie

Journal article  |  2019  |  AUTOPHAGY

BECN1/Beclin 1 is a critical protein in the initiation of autophagosome formation. Recent studies have shown that phosphorylation ..

Measuring translational diffusion of N-15-enriched biomolecules in complex solutions with a simplified H-1-N-15 HMQC-filtered BEST sequence

Shenggen Yao, Thomas G Meikle, Ashish Sethi, Frances Separovic, Jeffrey J Babon, David W Keizer

Journal article  |  2018  |  EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Pulsed-field gradient nuclear magnetic resonance has seen an increase in applications spanning a broad range of disciplines where ..

The molecular basis of JAK/STAT inhibition by SOCS1

Nicholas PD Liau, Artem Laktyushin, Isabelle S Lucet, James M Murphy, Shenggen Yao, Eden Whitlock, Kimberley Callaghan, Nicos A Nicola, Nadia J Kershaw, Jeffrey J Babon

Journal article  |  2018  |  NATURE COMMUNICATIONS

The SOCS family of proteins are negative-feedback inhibitors of signalling induced by cytokines that act via the JAK/STAT pathway...

Discovery and characterization of a sulfoquinovose mutarotase using kinetic analysis at equilibrium by exchange spectroscopy

Palika Abayakoon, James P Lingford, Yi Jin, Christopher Bengt, Gideon J Davies, Shenggen Yao, Ethan D Goddard-Borger, Spencer J Williams

Journal article  |  2018  |  BIOCHEMICAL JOURNAL

Bacterial sulfoglycolytic pathways catabolize sulfoquinovose (SQ), or glycosides thereof, to generate a three-carbon metabolite fo..

Lipidic Cubic Phase-Induced Membrane Protein Crystallization: Interplay Between Lipid Molecular Structure, Mesophase Structure and Properties, and Crystallogenesis

Alexandru Zabara, Thomas G Meikle, Raphael Trenker, Shenggen Yao, Janet Newman, Thomas S Peat, Frances Separovic, Charlotte E Conn, Melissa J Call, Matthew E Call, Ehud M Landau, Calum J Drummond

Journal article  |  2017  |  CRYSTAL GROWTH & DESIGN

Obtaining well-diffracting crystals of membrane proteins, which is crucial to the molecular-level understanding of their intrinsic..

Predicting the release profile of small molecules from within the ordered nanostructured lipidic bicontinuous cubic phase using translational diffusion coefficients determined by PFG-NMR

Thomas G Meikle, Shenggen Yao, Alexandru Zabara, Charlotte E Conn, Calum J Drummond, Frances Separovic

Journal article  |  2017  |  NANOSCALE

The ordered nanostructured lipidic bicontinuous cubic phase has demonstrated potential as a drug release material, due to its abil..

C-Terminal Modification and Multimerization Increase the Efficacy of a Proline-Rich Antimicrobial Peptide

Wenyi Li, Neil M O'Brien-Simpson, Shenggen Yao, Julien Tailhades, Eric C Reynolds, Raymond M Dawson, Laszlo Otvos, Mohammed Akhter Hossain, Frances Separovic, John D Wade

Journal article  |  2017  |  CHEMISTRY-A EUROPEAN JOURNAL

Two series of branched tetramers of the proline-rich antimicrobial peptide (PrAMP), Chex1-Arg20, were prepared to improve antibact..