Shenggen Yao

A solution NMR view of Lipidic Cubic Phases: Structure, dynamics, and beyond

Thomas G Meikle, David W Keizer, Frances Separovic, Shenggen Yao

Journal article  |  2022  |  BBA Advances

Nuclear magnetic resonance (NMR) spectroscopy is well-established nowadays for the elucidation of the 3D structures of proteins an..

NMR measurement of biomolecular translational and rotational motion for evaluating changes of protein oligomeric state in solution

Shenggen Yao, David W Keizer, Jeffrey J Babon, Frances Separovic

Journal article  |  2022  |  EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Defining protein oligomeric state and/or its changes in solution is of significant interest for many biophysical studies carried o..

NMR spectroscopy of lipidic cubic phases.

Sunnia Rajput, Shenggen Yao, David W Keizer, Marc-Antoine Sani, Frances Separovic

Journal article  |  2022  |  Biophys Rev

Lipidic cubic phase (LCP) structures have been used for stabilisation and crystallisation of membrane proteins and show promising ..

Water diffusion in complex systems measured by PGSE NMR using chemical shift selective stimulated echo: Elimination of magnetization exchange effects

Thomas Meikle, David W Keizer, Frances Separovic, Shenggen Yao

Journal article  |  2021  |  JOURNAL OF CHEMICAL PHYSICS

The interpretation of molecular translational diffusion as measured by pulsed gradient spin-echo NMR (PGSE NMR) can be complicated..

Chemical Exchange of Hydroxyl Groups in Lipidic Cubic Phases Characterized by NMR

Thomas G Meikle, David W Keizer, Jeffrey J Babon, Calum J Drummond, Frances Separovic, Charlotte E Conn, Shenggen Yao

Journal article  |  2021  |  JOURNAL OF PHYSICAL CHEMISTRY B

Proton transportation in proximity to the lipid bilayer membrane surface, where chemical exchange represents a primary pathway, is..

Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR

Thomas G Meikle, David W Keizer, Jeffrey J Babon, Calum J Drummond, Frances Separovic, Charlotte E Conn, Shenggen Yao

Journal article  |  2020  |  LANGMUIR

Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found..

Heteronuclear NMR spectroscopy of proteins encapsulated in cubic phase lipids

Thomas G Meikle, Ashish Sethi, David W Keizer, Jeffrey J Babon, Frances Separovic, Paul R Gooley, Charlotte E Conn, Shenggen Yao

Journal article  |  2019  |  JOURNAL OF MAGNETIC RESONANCE

Lipidic cubic phases, which form spontaneously via the self-assembly of certain lipids in an aqueous environment, are highly prosp..

Structural insights into BCL2 pro-survival protein interactions with the key autophagy regulator BECN1 following phosphorylation by STK4/MST1

Erinna F Lee, Nicholas A Smith, Tatiana P Soares da Costa, Nastaran Meftahi, Shenggen Yao, Tiffany J Harris, Sharon Tran, Anne Pettikiriarachchi, Matthew A Perugini, David W Keizer, Marco Evangelista, Brian J Smith, W Douglas Fairlie

Journal article  |  2019  |  AUTOPHAGY

BECN1/Beclin 1 is a critical protein in the initiation of autophagosome formation. Recent studies have shown that phosphorylation ..

Measuring translational diffusion of N-15-enriched biomolecules in complex solutions with a simplified H-1-N-15 HMQC-filtered BEST sequence

Shenggen Yao, Thomas G Meikle, Ashish Sethi, Frances Separovic, Jeffrey J Babon, David W Keizer

Journal article  |  2018  |  EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Pulsed-field gradient nuclear magnetic resonance has seen an increase in applications spanning a broad range of disciplines where ..

The molecular basis of JAK/STAT inhibition by SOCS1

Nicholas PD Liau, Artem Laktyushin, Isabelle S Lucet, James M Murphy, Shenggen Yao, Eden Whitlock, Kimberley Callaghan, Nicos A Nicola, Nadia J Kershaw, Jeffrey J Babon

Journal article  |  2018  |  NATURE COMMUNICATIONS

The SOCS family of proteins are negative-feedback inhibitors of signalling induced by cytokines that act via the JAK/STAT pathway...