Ben Gu

A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function

Kristen K Skarratt, Ben J Gu, Michael D Lovelace, Carol J Milligan, Leanne Stokes, Rachel Glover, Steven Petrou, James S Wiley, Stephen J Fuller

Journal article  |  2020  |  The FASEB Journal

P2X7 is an ATP-gated membrane ion channel that is expressed by multiple cell types. Brief exposure to ATP induces the opening of a..

Assays to Measure Purinoceptor Pore Dilation.

Ben J Gu, Pavan Avula, James S Wiley

Journal article  |  2020  |  Methods in molecular biology (Clifton, N.J.)

The P2X7 receptor is a classic purinoceptor/ion channel. After activated by ATP, it opens a cation selective channel, which dilate..

P2X7 receptor signaling during adult hippocampal neurogenesis

Hannah C Leeson, Tailoi Chan-Ling, Michael D Lovelace, Jeremy C Brownlie, Ben J Gu, Michael W Weible

Journal article  |  2019  |  Neural Regeneration Research

Targeting P2X7 receptors as a means for treating retinal disease

Erica L Fletcher, Anna Y Wang, Andrew I Jobling, Matthew V Rutar, Ursula Greferath, Ben Gu, Kirstan A Vessey

Journal article  |  2019  |  Drug Discovery Today

Age-related macular degeneration and glaucoma are the commonest causes of irreversible vision loss in industrialized countries. Th..

The local and systemic effects following subthreshold nanosecond laser treatment to the posterior retina

Quan Findlay, Andrew Ian Jobling, Kirstan Anne Vessey, Ursula Greferath, Pavan Avula, Ben Gu, Robyn H Guymer, Erica L Fletcher

Conference Proceedings  |  2019  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Hannah C Leeson, Tailoi Chan-Ling, Michael D Lovelace, Jeremy D Brownlie, Michael W Weible, Ben J Gu

Journal article  |  2019  |  Journal of Visualized Experiments

INNATE IMMUNITY IN ENDOPHTHALMITIS: THE ROLE OF PHAGOCYTOSIS AND THE P2X7 RECEPTOR

Andrew Hurley, Rosie Dawkins, Ben Gu, Xin Huang, Penelope Allen

Conference Proceedings  |  2018  |  CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY

P2X7 Receptors Regulate Phagocytosis and Proliferation in Adult Hippocampal and SVZ Neural Progenitor Cells: Implications for Inflammation in Neurogenesis

Hannah C Leeson, Maria A Kasherman, Tailoi Chan-Ling, Michael D Lovelace, Jeremy C Brownlie, Kelly M Toppinen, Ben J Gu, Michael W Weible

Journal article  |  2018  |  STEM CELLS

P2X7 as a scavenger receptor for innate phagocytosis in the brain

Ben J Gu, James S Wiley

Journal article  |  2018  |  British Journal of Pharmacology

The scavenger activity of the human P2X7 receptor differs from P2X7 pore function by insensitivity to antagonists, genetic variation and sodium concentration: Relevance to inflammatory brain diseases

Amber Ou, Ben J Gu, James S Wiley

Journal article  |  2018  |  Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

A systemic view of Alzheimer disease - insights from amyloid-beta metabolism beyond the brain

Jun Wang, Ben J Gu, Colin L Masters, Yan-Jiang Wang

Journal article  |  2017  |  Nature Reviews Neurology

Loss of Function of P2X7 Receptor Scavenger Activity in Aging Mice A Novel Model for Investigating the Early Pathogenesis of Age-Related Macular Degeneration

Kirstan A Vessey, Ben J Gu, Andrew I Jobling, Joanna A Phipps, Ursula Greferath, Mai X Tran, Michael A Dixon, Paul N Baird, Robyn H Guymer, James S Wiley, Erica L Fletcher

Journal article  |  2017  |  The American Journal of Pathology

Reduced Phagocytosis as a novel pathogenic mechanism in AMD pathogenesis

Emily Caruso, Ben Gu, Erica L Fletcher, Xin Huang, Pavan Avula, Kirstan Anne Vessey, James Saville Wiley, Robyn H Guymer

Conference Proceedings  |  2017  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Purinergic receptors P2RX4 and P2RX7 in familial multiple sclerosis

A Dessa Sadovnick, Ben J Gu, Anthony L Traboulsee, Cecily Q Bernales, Mary Encarnacion, Irene M Yee, Maria G Criscuoli, Xin Huang, Amber Ou, Carol J Milligan, Steven Petrou, James S Wiley, Carles Vilarino-Guell

Journal article  |  2017  |  Human Mutation

Innate phagocytosis by peripheral blood monocytes is altered in Alzheimer's disease

Ben J Gu, Xin Huang, Amber Ou, Alan Rembach, Christopher Fowler, Pavan K Avula, Adam Horton, James D Doecke, Victor L Villemagne, S Lance Macaulay, Paul Maruff, Erica L Fletcher, Robyn Guymer, James S Wiley, Colin L Masters

Journal article  |  2016  |  Acta Neuropathologica

A rare P2X7 variant Arg307Gln with absent pore formation function protects against neuroinflammation in multiple sclerosis

Ben J Gu, Judith Field, Sebastien Dutertre, Amber Ou, Trevor J Kilpatrick, Jeannette Lechner-Scott, Rodney Scott, Rodney Lea, Bruce V Taylor, Jim Stankovich, Helmut Butzkueven, Melissa Gresle, Simon M Laws, Steven Petrou, Sabine Hoffjan, Denis A Akkad, Colin A Graham, Stanley Hawkins, Anna Glaser, Sahl Khalid Bedri, et al.

Journal article  |  2015  |  Human Molecular Genetics

Shear stress modulates endothelial KLF2 through activation of P2X4

R Sathanoori, F Rosi, BJ Gu, JS Wiley, CE Mueller, B Olde, D Erlinge

Journal article  |  2015  |  Purinergic Signalling

P2X7 Receptors Mediate Innate Phagocytosis by Human Neural Precursor Cells and Neuroblasts

Michael D Lovelace, Ben J Gu, Steven S Eamegdool, Michael W Weible, James S Wiley, David G Allen, Tailoi Chan-Ling

Journal article  |  2015  |  Stem Cells

A Quantitative Method for Measuring Innate Phagocytosis by Human Monocytes Using Real-Time Flow Cytometry

Ben J Gu, Chun Sun, Stephen Fuller, Kristen K Skarratt, Steven Petrou, James S Wiley

Journal article  |  2014  |  Cytometry Part A

A rare functional haplotype of the P2RX4 and P2RX7 genes leads to loss of innate phagocytosis and confers increased risk of age-related macular degeneration

Ben J Gu, Paul N Baird, Kirstan A Vessey, Kristen K Skarratt, Erica L Fletcher, Stephen J Fuller, Andrea J Richardson, Robyn H Guymer, James S Wiley

Journal article  |  2013  |  FASEB Journal

Non-synonymous polymorphisms in the P2RX (4) are related to bone mineral density and osteoporosis risk in a cohort of Dutch fracture patients

Anke Wesselius, Martijn JL Bours, Niklas R Jorgensen, James Wiley, Ben Gu, Svenjhalmar van Helden, Lodewijk van Rhijn, Pieter C Dagnelie

Journal article  |  2013  |  Purinergic Signalling

Epistasis with HLA DR3 implicates the P2X7 receptor in the pathogenesis of primary Sjogren's syndrome

Susan Lester, Leanne Stokes, Kristen K Skarratt, Ben J Gu, Kathy L Sivils, Christopher J Lessard, James S Wiley, Maureen Rischmueller

Journal article  |  2013  |  Arthritis Research & Therapy

A new role for the P2X7 receptor: a scavenger receptor for bacteria and apoptotic cells in the absence of serum and extracellular ATP

James S Wiley, Ben J Gu

Journal article  |  2012  |  Purinergic Signalling

P2X7 Receptor-mediated Scavenger Activity of Mononuclear Phagocytes toward Non-opsonized Particles and Apoptotic Cells Is Inhibited by Serum Glycoproteins but Remains Active in Cerebrospinal Fluid

Ben J Gu, James A Duce, Valentina A Valova, Bruce Wong, Ashley I Bush, Steven Petrou, James S Wiley

Journal article  |  2012  |  Journal of Biological Chemistry

A Loss-of-Function Polymorphism in the Human P2X4 Receptor Is Associated With Increased Pulse Pressure

Leanne Stokes, Katrina Scurrah, Justine A Ellis, Brett A Cromer, Kristen K Skarratt, Ben J Gu, Stephen B Harrap, James S Wiley

Journal article  |  2011  |  Hypertension

The human P2X7 receptor and its role in innate immunity

JS Wiley, R Sluyter, BJ Gu, L Stokes, SJ Fuller

Journal article  |  2011  |  Tissue Antigens

P2X(7) Is a Scavenger Receptor for Apoptotic Cells in the Absence of Its Ligand, Extracellular ATP

Ben J Gu, Bernadette M Saunders, Steven Petrou, James S Wiley

Journal article  |  2011  |  Journal of Immunology

Functional significance of P2RX7 polymorphisms associated with affective mood disorders

Ronald Sluyter, Leanne Stokes, Stephen J Fuller, Kristen K Skarratt, Ben J Gu, James S Wiley

Journal article  |  2010  |  Journal of Psychiatric Research

Identification of the promoter region of the P2RX4 gene

Ben J Gu, Chun Sun, Valentina A Valova, Kristen K Skarratt, James S Wiley

Journal article  |  2010  |  Molecular Biology Reports

Two haplotypes of the P2X(7) receptor containing the Ala-348 to Thr polymorphism exhibit a gain-of-function effect and enhanced interleukin-1 beta secretion

Leanne Stokes, Stephen J Fuller, Ronald Sluyter, Kristen K Skarratt, Ben J Gu, James S Wiley

Journal article  |  2010  |  FASEB Journal

The P2X(7)-nonmuscle myosin membrane complex regulates phagocytosis of nonopsonized particles and bacteria by a pathway attenuated by extracellular ATP

Ben J Gu, Bernadette M Saunders, Claudia Jursik, James S Wiley

Journal article  |  2010  |  Blood

Extracellular ATP dissociates nonmuscle myosin from P2X(7) complex: this dissociation regulates P2X(7) pore formation

Ben J Gu, Catherine Rathsam, Leanne Stokes, Andrew B McGeachie, James S Wiley

Journal article  |  2009  |  American Journal of Physiology-Cell Physiology

Genetics of the P2X7 receptor and human disease

Stephen J Fuller, Leanne Stokes, Kristen K Skarratt, Ben J Gu, James S Wiley

Journal article  |  2009  |  Purinergic Signalling

A quantitative method for routine measurement of cell surface P2X(7) receptor function in leucocyte subsets by two-colour time-resolved flow cytometry

Claudia Jursik, Ronald Sluyter, Jennifer G Georgiou, Stephen J Fuller, James S Wiley, Ben J Gu

Journal article  |  2007  |  Journal of Immunological Methods

Rapid ATP-induced release of matrix metalloproteinase 9 is mediated by the P2X(7) receptor

Ben J Gu, James S Wiley

Journal article  |  2006  |  Blood

A Thr(357) to Ser polymorphism in homozygous and compound heterozygous subjects causes absent or reduced P2X(7) function and impairs ATP-induced mycobacterial killing by macrophages

AN Shemon, R Sluyter, SL Fernando, AL Clarke, LP Dao-Ung, KK Skarratt, BM Saunders, KS Tan, BJ Gu, SJ Fuller, WJ Britton, S Petrou, JS Wiley

Journal article  |  2006  |  The Journal of biological chemistry

A Thr357to Ser polymorphism in homozygous and compound heterozygous subjects causes absent or reduced P2X7function and impairs ATP-induced mycobacterial killing by macrophages

AN Shemon, R Sluyter, SL Fernando, AL Clarke, LP Dao-Ung, KK Skarratt, BM Saunders, ST Khai, BJ Gu, SJ Fuller, WJ Britton, S Petrou, JS Wiley

Journal article  |  2006  |  Journal of Biological Chemistry

A 5 ' intronic splice site polymorphism leads to a nulll allele of the P2X(7) gene in 1-2% of the Caucasian population

KK Skarratt, SJ Fuller, R Sluyter, LP Dao-Ung, BJ Gu, JS Wiley

Journal article  |  2005  |  FEBS Letters

An Arg(307) to Gln polymorphism within the ATP-binding site causes loss of function of the human P2X(7) receptor

BJ Gu, R Sluyter, KK Skarratt, AN Shemon, LP Dao-Ung, SJ Fuller, JA Barden, AL Clarke, S Petrou, JS Wiley

Journal article  |  2004  |  Journal of Biological Chemistry

An Ile-568 to Asn polymorphism prevents normal trafficking and function of the human P2X(7) receptor

JS Wiley, LP Dao-Ung, CP Li, AN Shemon, BJ Gu, ML Smart, SJ Fuller, JA Barden, S Petrou, R Sluyter

Journal article  |  2003  |  Journal of Biological Chemistry

Specific detection of non-functional human P2X(7) receptors in HEK293 cells and B-lymphocytes

JA Barden, R Sluyter, BJ Gu, JS Wiley

Journal article  |  2003  |  FEBS Letters

P2X7 receptor cell surface expression and cytolytic pore formation are regulated by a distal C-terminal region

ML Smart, B Gu, RG Panchal, J Wiley, B Cromer, DA Williams, S Petrou

Journal article  |  2003  |  Journal of Biological Chemistry

A loss-of-function polymorphic mutation in the cytolytic P2X7 receptor gene and chronic lymphocytic leukaemia: a molecular study

JS Wiley, LP Dao-Ung, BJ Gu, R Sluyter, AN Shemon, CP Li, J Taper, J Gallo, A Manoharan

Journal article  |  2002  |  The Lancet

Point mutations confer loss of ATP-induced human P2X(7) receptor function

RA Worthington, ML Smart, BJ Gu, DA Williams, S Petrou, JS Wiley, JA Barden

Journal article  |  2002  |  FEBS Letters

P2Y(11) receptor expression by human lymphocytes: evidence for two cAMP-linked purinoceptors

AD Conigrave, KC Fernando, B Gu, V Tasevski, WY Zhang, BM Luttrell, JS Wiley

Journal article  |  2001  |  European Journal of Pharmacology

Impaired transendothelial migration of B-CLL lymphocytes: a defect linked to low L-selectin expression

B Gu, LP Dao, J Wiley

Journal article  |  2001  |  Leukemia & Lymphoma

Genetic polymorphisms of the human P2X(7) receptor and relationship to function

JS Wiley, BJ Gu, WY Zhang, RA Worthington, P Dao-Ung, AN Shemon, R Sluyter, S Liang, JA Barden

Conference Proceedings  |  2001  |  Drug Development Research

A Glu-496 to Ala polymorphism leads to loss of function of the human P2X(7) receptor

BJ Gu, WY Zhang, RA Worthington, R Sluyter, P Dao-Ung, S Petrou, JA Barden, JS Wiley

Journal article  |  2001  |  Journal of Biological Chemistry

Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors.

BJ Gu, WY Zhang, LJ Bendall, IP Chessell, GN Buell, JS Wiley

Journal article  |  2000  |  American Journal of Physiology-Cell Physiology

Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellul..

Transendothelial migration of lymphocytes in chronic lymphocytic leukaemia is impaired and involves down-regulation of both L-selectin and CD23

JR Chen, BJ Gu, LP Dao, CJ Bradley, SP Mulligan, JS Wiley

Journal article  |  1999  |  British Journal of Haematology

Chronic lymphocytic leukaemia (B-CLL) is characterized by a progressive accumulation of B lymphocytes in blood and bone marrow and..

Adenosine triphosphate-induced shedding of CD23 and L-selectin (CD62L) from lymphocytes is mediated by the same receptor but different metalloproteases

B Gu, LJ Bendall, JS Wiley

Journal article  |  1998  |  Blood

Broad Applications of Multi-Colour Time-Resolved Flow Cytometry

Ben J., James S.

Book Chapter

P2X7 IS AN ARCHAIC SCAVENGER RECEPTOR RECOGNIZING APOPTOTIC NEUROBLASTS IN EARLY HUMAN NEUROGENESIS

Journal article  |  Receptors & Clinical Investigation