Angelique Haynes

Arginine-rich C9ORF72 ALS proteins stall ribosomes in a manner distinct from a canonical ribosome-associated quality control substrate.

Viacheslav Kriachkov, Angelique R Ormsby, Eric P Kusnadi, Hamish EG McWilliam, Justine D Mintern, Shanika L Amarasinghe, Matthew E Ritchie, Luc Furic, Danny M Hatters

Journal article  |  2023  |  J Biol Chem

Hexanucleotide expansion mutations in C9ORF72 are a frequent cause of amyotrophic lateral sclerosis. We previously reported that l..

Protein painting reveals pervasive remodeling of conserved proteostasis machinery in response to pharmacological stimuli

Dezerae Cox, Angelique R Ormsby, Gavin E Reid, Danny M Hatters

Journal article  |  2022  |  NPJ SYSTEMS BIOLOGY AND APPLICATIONS

The correct spatio-temporal organization of the proteome is essential for cellular homeostasis. However, a detailed mechanistic un..

Sequence grammar underlying the unfolding and phase separation of globular proteins

Kiersten M Ruff, Yoon Hee Choi, Dezerae Cox, Angelique R Ormsby, Yoochan Myung, David B Ascher, Sheena E Radford, Rohit V. Pappu, Danny M Hatters

Journal article  |  2022  |  MOLECULAR CELL

Aberrant phase separation of globular proteins is associated with many diseases. Here, we use a model protein system to understand..

A biosensor of protein foldedness identifies increased "holdase" activity of chaperones in the nucleus following increased cytosolic protein aggregation.

Candice B Raeburn, Angelique R Ormsby, Dezerae Cox, Chloe A Gerak, Christian Makhoul, Nagaraj S Moily, Simon Ebbinghaus, Alex Dickson, Gawain McColl, Danny M Hatters

Journal article  |  2022  |  J Biol Chem

Chaperones and other quality control machinery guard proteins from inappropriate aggregation, which is a hallmark of neurodegenera..

Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control and RNA

Angelique R Ormsby, Dezerae Cox, James Daly, David Priest, Elizabeth Hinde, Danny M Hatters

Journal article  |  2020  |  PLOS ONE

Mutations that cause Huntington's Disease involve a polyglutamine (polyQ) sequence expansion beyond 35 repeats in exon 1 of Huntin..

Arginine in C9ORF72 Dipolypeptides Mediates Promiscuous Proteome Binding and Multiple Modes of Toxicity

Mona Radwan, Ching-Seng Ang, Angelique R Ormsby, Dezerae Cox, James C Daly, Gavin E Reid, Danny M Hatters

Journal article  |  2020  |  MOLECULAR & CELLULAR PROTEOMICS

C9ORF72-associated Motor Neuron Disease patients feature abnormal expression of 5 dipeptide repeat (DPR) polymers. Here we used qu..

Arginine in C9ORF72 Dipolypeptides Mediates Promiscuous Proteome Binding and Multiple Modes of Toxicity.

Mona Radwan, Ching-Seng Ang, Angelique R Ormsby, Dezerae Cox, James C Daly, Gavin E Reid, Danny M Hatters

Journal article  |  2020  |  Mol Cell Proteomics

C9ORF72-associated Motor Neuron Disease patients feature abnormal expression of 5 dipeptide repeat (DPR) polymers. Here we used qu..

Widespread remodeling of proteome solubility in response to different protein homeostasis stresses

Xiaojing Sui, Douglas EV Pires, Angelique R Ormsby, Dezerae Cox, Shuai Nie, Giulia Vecchi, Michele Vendruscolo, David B Ascher, Gavin E Reid, Danny M Hatters

Journal article  |  2020  |  Proceedings of the National Academy of Sciences of the United States of America

The accumulation of protein deposits in neurodegenerative diseases has been hypothesized to depend on a metastable subproteome vul..

Tadpole-like Conformations of Huntingtin Exon 1 Are Characterized by Conformational Heterogeneity that Persists regardless of Polyglutamine Length

Estella A Newcombe, Kiersten M Ruff, Ashish Sethi, Angelique R Ormsby, Yasmin M Ramdzan, Archa Fox, Anthony W Purcell, Paul R Gooley, Rohit Pappu, Danny M Hatters

Journal article  |  2018  |  JOURNAL OF MOLECULAR BIOLOGY

Soluble huntingtin exon 1 (Httex1) with expanded polyglutamine (polyQ) engenders neurotoxicity in Huntington's disease. To uncover..

Measuring proteostasis capacity using transiently transfected bait proteins by flow cytometry

Danny Hatters, Rebecca Wood, Candice Raeburn, Angelique Ormsby, Mikael Oliveberg, Alex Dickson, Danny Hatters

Journal article  |  2018  |  Protocol Exchange