Christopher Tonkin

Metabolomic Analysis of Toxoplasma gondii Tachyzoites.

Elizabeth FB King, Simon A Cobbold, Alessandro D Uboldi, Christopher J Tonkin, Malcolm J McConville

Journal article  |  2020  |  Methods in molecular biology (Clifton, N.J.)

This protocol describes the use of 13C-stable isotope labeling, combined with metabolite profiling, to investigate the metabolism ..

Protein Kinase A Is Essential for Invasion of Plasmodium falciparum into Human Erythrocytes

Mary-Louise Wilde, Tony Triglia, Danushka Marapana, Jennifer K Thompson, Alexei A Kouzmitchev, Hayley E Bullen, Paul R Gilson, Alan F Cowman, Christopher J Tonkin

Journal article  |  2019  |  mBio

Understanding the mechanisms behind host cell invasion by Plasmodium falciparum remains a major hurdle to developing antimalarial ..

Impaired social behaviour and molecular mediators of associated neural circuits during chronic Toxoplasma gondii infection in female mice

Shiraz Tyebji, Simona Seizova, Alexandra L Garnham, Anthony J Hannan, Christopher J Tonkin

Journal article  |  2019  |  Brain, Behavior, and Immunity

Toxoplasma gondii (T. gondii) is a neurotropic parasite that is associated with various neuropsychiatric disorders. Rodents infect..

Toxoplasma gondii bradyzoites induce transcriptional changes to host cells and prevent IFNγ-mediated cell death

Simona Seizova, Alexandra Garnham, Michael Coffey, Lachlan Whitehead, Kelly Rogers, Christopher Tonkin

Journal article  |  2019

Summary Toxoplasma gondii , the causative agent of toxoplasmosis, lies dormant for life and is a reservoir for disease reactivatio..

An apically located hybrid guanylate cyclase-ATPase is critical for the initiation of Ca2 signaling and motility in Toxoplasma gondii

Luning Yang, Alessandro D Uboldi, Simona Seizova, Mary-Louise Wilde, Michael J Coffey, Nicholas J Katris, Yoshiki Yamaryo-Botte, Martina Kocan, Ross AD Bathgate, Rebecca J Stewart, Malcolm J McConville, Philip E Thompson, Cyrille Y Botte, Christopher J Tonkin

Journal article  |  2019  |  Journal of Biological Chemistry

Characterization of the ATP4 ion pump in Toxoplasma gondii

Adele M Lehane, Adelaide SM Dennis, Katherine O Bray, Dongdi Li, Esther Rajendran, James M McCoy, Hillary M McArthur, Markus Winterberg, Farid Rahimi, Christopher J Tonkin, Kiaran Kirk, Giel G van Dooren

Journal article  |  2019  |  Journal of Biological Chemistry

Evaluation of 4-Amino 2-Anilinoquinazolines against Plasmodium and Other Apicomplexan Parasites In Vitro and in a P. falciparum Humanized NOD-scid IL2R gamma(null) Mouse Model of Malaria

Paul R Gilson, William Nguyen, William A Poole, Jose E Teixeira, Jennifer K Thompson, Kaiyuan Guo, Rebecca J Stewart, Trent D Ashton, Karen L White, Laura M Sanz, Francisco-Javier Gamo, Susan A Charman, Sergio Wittlin, James Duffy, Christopher J Tonkin, Wai-Hong Tham, Brendan S Crabb, Brian M Cooke, Christopher D Huston, Alan F Cowman, et al.

Journal article  |  2019  |  Antimicrobial Agents and Chemotherapy

Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection

Sachin Khurana, Michael J Coffey, Alan John, Alessandro D Uboldi, My-Hang Huynh, Rebecca J Stewart, Vern B Carruthers, Christopher J Tonkin, Ethan D Goddard-Borger, Nichollas E Scott

Journal article  |  2019  |  Journal of Biological Chemistry

Structure of Plasmodium falciparum Rh5-CyRPA-Ripr invasion complex

Wilson Wong, Rick Huang, Sebastien Menant, Chuan Hong, Jarrod J Sandow, Richard W Birkinshaw, Julie Healer, Anthony N Hodder, Usheer Kanjee, Christopher J Tonkin, Denise Heckmann, Vladislav Soroka, Teit Max Moscote Sogaard, Thomas Jorgensen, Manoj T Duraisingh, Peter E Czabotar, Willem A de Jongh, Wai-Hong Tham, Andrew I Webb, Zhiheng Yu, et al.

Journal article  |  2019  |  Nature

Toxoplasmosis: A pathway to neuropsychiatric disorders

Shiraz Tyebji, Simona Seizova, Anthony J Hannan, Christopher J Tonkin

Journal article  |  2019  |  Neuroscience & Biobehavioral Reviews

Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase

Azadeh Seidi, Linden S Muellner-Wong, Esther Rajendran, Edwin T Tjhin, Laura F Dagley, Vincent YT Aw, Pierre Faou, Andrew I Webb, Christopher J Tonkin, Giel G van Dooren

Journal article  |  2018  |  eLife

Protein kinase A negatively regulates Ca2 signalling in Toxoplasma gondii

Alessandro D Uboldi, Mary-Louise Wilde, Emi A McRae, Rebecca J Stewart, Laura F Dagley, Luning Yang, Nicholas J Katris, Sanduni Hapuarachchi, Michael J Coffey, Adele M Lehane, Cyrille Y Botte, Ross F Waller, Andrew Webb, Malcolm J McConville, Christopher J Tonkin

Journal article  |  2018  |  PLOS Biology

Aspartyl Protease 5 Matures Dense Granule Proteins That Reside at the Host-Parasite Interface in Toxoplasma gondii

Michael J Coffey, Laura F Dagley, Simona Seizova, Eugene A Kapp, Giuseppe Infusini, David S Roos, Justin A Boddey, Andrew I Webb, Christopher J Tonkin

Journal article  |  2018  |  mBio

Plasmodium falciparum subtilisin-like ookinete protein SOPT plays an important and conserved role during ookinete infection of the Anopheles stephensi midgut

Jennifer S Armistead, Charlie Jennison, Matthew T O'Neill, Sash Lopaticki, Peter Liehl, Kirsten K Hanson, Takeshi Annoura, Pravin Rajasekaran, Sara M Erickson, Christopher J Tonkin, Shahid M Khan, Maria M Mota, Justin A Boddey

Journal article  |  2018  |  Molecular Microbiology

MYR1-Dependent Effectors Are the Major Drivers of a Host Cell's Early Response to Toxoplasma, Including Counteracting MYR1-Independent Effects

Adit Naor, Michael W Panas, Nicole Marino, Michael J Coffey, Christopher J Tonkin, John C Boothroyd

Journal article  |  2018  |  mBio

Aspartyl Protease 5 matures virulence factors found at the host-parasite interface in Toxoplasma gondii

Michael Coffey, Laura Dagley, Eugene Kapp, Giuseppe Infusini, Justin Boddey, Andrew Webb, Christopher Tonkin

Journal article  |  2018

Abstract Toxoplasma gondii infects approximately 30% of the world’s population, causing disease primarily during pregnancy and in ..

Development of a Novel CD4( ) TCR Transgenic Line That Reveals a Dominant Role for CD8( ) Dendritic Cells and CD40 Signaling in the Generation of Helper and CTL Responses to Blood-Stage Malaria

Daniel Fernandez-Ruiz, Lei Shong Lau, Nazanin Ghazanfari, Claerwen M Jones, Wei Yi Ng, Gayle M Davey, Dorothee Berthold, Lauren Holz, Yu Kato, Matthias H Enders, Ganchimeg Bayarsaikhan, Sanne H Hendriks, Lianne IM Lansink, Jessica A Engel, Megan SF Soon, Kylie R James, Anton Cozijnsen, Vanessa Mollard, Alessandro D Uboldi, Christopher J Tonkin, et al.

Journal article  |  2017  |  The Journal of Immunology

The Molecular Basis of Erythrocyte Invasion by Malaria Parasites

Alan F Cowman, Christopher J Tonkin, Wai-Hong Tham, Manoj T Duraisingh

Journal article  |  2017  |  Cell Host & Microbe

A forward genetic screen identifies a negative regulator of rapid Ca2 -dependent cell egress (MS1) in the intracellular parasite Toxoplasma gondii

James M McCoy, Rebecca J Stewart, Alessandro D Uboldi, Dongdi Li, Jan Schroder, Nicollas E Scott, Anthony T Papenfuss, Adele M Lehane, Leonard J Foster, Christopher J Tonkin

Journal article  |  2017  |  Journal of Biological Chemistry

Analysis of Ca2 mediated signaling regulating Toxoplasma infectivity reveals complex relationships between key molecules

Rebecca J Stewart, Lachlan Whitehead, Brunda Nijagal, Brad E Sleebs, Guillaume Lessene, Malcolm J McConville, Kelly L Rogers, Christopher J Tonkin

Journal article  |  2017  |  Cellular Microbiology

Truncated Latrunculins as Actin Inhibitors Targeting Plasmodium falciparum Motility and Host Cell Invasion

Swapna Johnson, Raphael Rahmani, Damien R Drew, Melanie J Williams, Mark Wilkinson, Yan Hong Tan, Johnny X Huang, Christopher J Tonkin, James G Beeson, Jake Baum, Brian J Smith, Jonathan B Baell

Journal article  |  2016  |  Journal of Medicinal Chemistry

Role of the ER and Golgi in protein export by Apicomplexa

Michael J Coffey, Charlie Jennison, Christopher J Tonkin, Justin A Boddey

Journal article  |  2016  |  Current Opinion in Cell Biology

Phosphorylation of SNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii

Rebecca J Stewart, David JP Ferguson, Lachlan Whitehead, Clare H Bradin, Hong J Wu, Christopher J Tonkin

Journal article  |  2016  |  Traffic

Regulation of Starch Stores by a Ca2 -Dependent Protein Kinase Is Essential for Viable Cyst Development in Toxoplasma gondii

Alessandro D Uboldi, James M McCoy, Martin Blume, Motti Gerlic, David JP Ferguson, Laura F Dagley, Cherie T Beahan, David I Stapleton, Paul R Gooley, Antony Bacic, Seth L Masters, Andrew I Webb, Malcolm J McConville, Christopher J Tonkin

Journal article  |  2015  |  Cell Host & Microbe

An aspartyl protease defines a novel pathway for export of Toxoplasma proteins into the host cell

Michael J Coffey, Brad E Sleebs, Alessandro D Uboldi, Alexandra Garnham, Magdalena Franco, Nicole D Marino, Michael W Panas, David JP Ferguson, Marta Enciso, Matthew T O'Neill, Sash Lopaticki, Rebecca J Stewart, Grant Dewson, Gordon K Smyth, Brian J Smith, Seth L Masters, John C Boothroyd, Justin A Boddey, Christopher J Tonkin

Journal article  |  2015  |  eLife

Disassembly activity of actin-depolymerizing factor (ADF) is associated with distinct cellular processes in apicomplexan parasites

Silvia Haase, Dennis Zimmermann, Maya A Olshina, Mark Wilkinson, Fabio Fisher, Yan Hong Tan, Rebecca J Stewart, Christopher J Tonkin, Wilson Wong, David R Kovar, Jake Baum

Journal article  |  2015  |  Molecular Biology of the Cell

Two Essential Light Chains Regulate the MyoA Lever Arm To Promote Toxoplasma Gliding Motility

Melanie J Williams, Hernan Alonso, Marta Enciso, Saskia Egarter, Lilach Sheiner, Markus Meissner, Boris Striepen, Brian J Smith, Christopher J Tonkin

Journal article  |  2015  |  mBio

Rhomboid proteases in invasion and replication of Apicomplexa

Rebecca J Stewart, Christopher J Tonkin

Journal article  |  2015  |  Molecular Microbiology

Identification of Potent Phosphodiesterase Inhibitors that Demonstrate Cyclic Nucleotide-Dependent Functions in Apicomplexan Parasites

Brittany L Howard, Katherine L Harvey, Rebecca J Stewart, Mauro F Azevedo, Brendan S Crabb, Ian G Jennings, Paul R Sanders, David T Manallack, Philip E Thompson, Christopher J Tonkin, Paul R Gilson

Journal article  |  2015  |  ACS Chemical Biology

An Overexpression Screen of Toxoplasma gondii Rab-GTPases Reveals Distinct Transport Routes to the Micronemes

Katrin Kremer, Dirk Kamin, Eva Rittweger, Jonathan Wilkes, Halley Flammer, Sabine Mahler, Joanne Heng, Christopher J Tonkin, Gordon Langsley, Stefan W Hell, Vernon B Carruthers, David JP Ferguson, Markus Meissner

Journal article  |  2013  |  PLoS Pathogens

TgCDPK3 Regulates Calcium-Dependent Egress of Toxoplasma gondii from Host Cells

James M McCoy, Lachlan Whitehead, Giel G van Dooren, Christopher J Tonkin

Journal article  |  2012  |  PLoS Pathogens

Mitochondrial Metabolism of Glucose and Glutamine Is Required for Intracellular Growth of Toxoplasma gondii

James I MacRae, Lilach Sheiner, Amsha Nahid, Christopher Tonkin, Boris Striepen, Malcolm J McConville

Journal article  |  2012  |  Cell Host & Microbe

Calcium and Repression in Malaria Sex: Knowing When the Time Is Right

James M McCoy, Christopher J Tonkin

Journal article  |  2012  |  Cell Host & Microbe

Spatial Localisation of Actin Filaments across Developmental Stages of the Malaria Parasite

Fiona Angrisano, David T Riglar, Angelika Sturm, Jennifer C Volz, Michael J Delves, Elizabeth S Zuccala, Lynne Turnbull, Chaitali Dekiwadia, Maya A Olshina, Danushka S Marapana, Wilson Wong, Vanessa Mollard, Clare H Bradin, Christopher J Tonkin, Peter W Gunning, Stuart A Ralph, Cynthia B Whitchurch, Robert E Sinden, Alan F Cowman, Geoffrey I McFadden, et al.

Journal article  |  2012  |  PLoS One

Quantitative in vivo Analyses Reveal Calcium-dependent Phosphorylation Sites and Identifies a Novel Component of the Toxoplasma Invasion Motor Complex

Thomas Nebl, Judith Helena Prieto, Eugene Kapp, Brian J Smith, Melanie J Williams, John R Yates, Alan F Cowman, Christopher J Tonkin

Journal article  |  2011  |  PLoS Pathogens

Sequencing and Analysis of JC Virus DNA From Natalizumab-Treated PML Patients

Carl E Reid, Huo Li, Gargi Sur, Paul Carmillo, Steven Bushnell, Rich Tizard, Michele McAuliffe, Christopher Tonkin, Kenneth Simon, Susan Goelz, Paola Cinque, Leonid Gorelik, John P Carulli

Journal article  |  2011  |  The Journal of Infectious Diseases

BACKGROUND: Progressive multifocal leukoencephalopathy (PML) in natalizumab-treated MS patients is linked to JC virus (JCV) infect..

Progressive multifocal leukoencephalopathy (PML) development is associated with mutations in JC virus capsid protein VP1 that change its receptor specificity.

Leonid Gorelik, Carl Reid, Manuela Testa, Margot Brickelmaier, Simona Bossolasco, Annamaria Pazzi, Arabella Bestetti, Paul Carmillo, Ewa Wilson, Michele McAuliffe, Christopher Tonkin, John P Carulli, Alexey Lugovskoy, Adriano Lazzarin, Shamil Sunyaev, Kenneth Simon, Paola Cinque

Journal article  |  2011  |  The Journal of Infectious Diseases

Progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease caused by JC virus (JCV) infection of oligodendroc..

A Tail of Division

Alan F Cowman, Christopher J Tonkin

Journal article  |  2011  |  Science

Characterization of Two Putative Protein Translocation Components in the Apicoplast of Plasmodium falciparum

Ming Kalanon, Christopher J Tonkin, Geoffrey I McFadden

Journal article  |  2009  |  Eukaryotic Cell

Sir2 Paralogues Cooperate to Regulate Virulence Genes and Antigenic Variation in Plasmodium falciparum

Christopher J Tonkin, Celine K Carret, Manoj T Duraisingh, Till S Voss, Stuart A Ralph, Mirja Hommel, Michael F Duffy, Liliana Mancio da Silva, Artur Scherf, Alasdair Ivens, Terence P Speed, James G Beeson, Alan F Cowman

Journal article  |  2009  |  PLoS Biology

Evolution of malaria parasite plastid targeting sequences

Christopher J Tonkin, Bernardo J Foth, Stuart A Ralph, Nicole Struck, Alan F Cowman, Geoffrey I McFadden

Journal article  |  2008  |  Proceedings of the National Academy of Sciences of the United States of America

Alterations in local chromatin environment are involved in silencing and activation of subtelomeric var genes in Plasmodium falciparum

Till S Voss, Christopher J Tonkin, Allison J Marty, Jennifer K Thompson, Julie Healer, Brendan S Crabb, Alan F Cowman

Journal article  |  2007  |  Molecular Microbiology

High level expression of proteins using sequences from the ferritin heavy chain gene locus.

Holly L Prentice, Christopher JD Tonkin, Louisa Caamano, William P Sisk

Journal article  |  2007  |  Journal of Biotechnology

An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor pro..

Molecular mechanism for switching of P-falciparum invasion pathways into human erythrocytes

J Stubbs, KM Simpson, T Triglia, D Plouffe, CJ Tonkin, MT Duraisingh, AG Maier, EA Winzeler, AF Cowman

Journal article  |  2005  |  Science

Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method (vol 137, pg 13, 2004)

CJ Tonkin, GG van Dooren, TP Spurck, NS Struck, RT Good, E Handman, AF Cowman, GI McFadden

Journal article  |  2004  |  Molecular and Biochemical Parasitology

Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method

CJ Tonkin, GG van Dooren, TP Spurck, NS Struck, RT Good, E Handman, AF Cowman, GI McFadden

Journal article  |  2004  |  Molecular and Biochemical Parasitology

Metabolic maps and functions of the Plasmodium falciparum apicoplast

SA Ralph, GG van Dooren, RF Waller, MJ Crawford, MJ Fraunholz, BJ Foth, CJ Tonkin, DS Roos, GI McFadden

Journal article  |  2004  |  Nature Reviews Microbiology

Insensitivity to anti-müllerian hormone due to a mutation in the human anti-müllerian hormone receptor.

S Imbeaud, E Faure, I Lamarre, MG Mattéi, N di Clemente, R Tizard, D Carré-Eusèbe, C Belville, L Tragethon, C Tonkin, J Nelson, M McAuliffe, JM Bidart, A Lababidi, N Josso, RL Cate, JY Picard

Journal article  |  1995  |  Nature Genetics

Anti-Müllerian hormone (AMH) and its receptor are involved in the regression of Müllerian ducts in male fetuses. We have now clone..