Christopher Tonkin

Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii.

Mary-Louise Wilde, Ushma Ruparel, Theresa Klemm, V Vern Lee, Dale J Calleja, David Komander, Christopher J Tonkin

Journal article  |  2023  |  Life Sci Alliance

The phylum Apicomplexa contains several parasitic species of medical and agricultural importance. The ubiquitination machinery rem..

Conservation, abundance, glycosylation profile, and localization of the TSP protein family in Cryptosporidium parvum.

Alan John, Stefanie M Bader, Niccolay Madiedo Soler, Kharizta Wiradiputri, Swapnil Tichkule, Sean T Smyth, Stuart A Ralph, Aaron R Jex, Nichollas E Scott, Christopher J Tonkin, Ethan D Goddard-Borger

Journal article  |  2023  |  J Biol Chem

Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of v..

A CRISPR upgrade unlocks Toxoplasma gene function

Aurelie Tsee Dawson, Christopher J Tonkin

Journal article  |  2022  |  TRENDS IN PARASITOLOGY

Forward genetic screens are invaluable in describing gene function. CRISPR has reinvigorated phenotypic screens in Toxoplasma - a ..

3, 2, 1, go! Cryptosporidium counts down to sex

Aaron R Jex, Christopher J Tonkin, Stuart A Ralph

Journal article  |  2022  |  PLOS BIOLOGY

Cryptosporidium is a leading cause of death from childhood diarrhea, but its biology is poorly understood. A recent study in PLOS ..

Transcriptional modification of host cells harboring Toxoplasma gondii bradyzoites prevents IFN gamma-mediated cell death

Simona Seizova, Ushma Ruparel, Alexandra L Garnham, Stefanie M Bader, Alessandro D Uboldi, Michael J Coffey, Lachlan W Whitehead, Kelly L Rogers, Christopher J Tonkin

Journal article  |  2022  |  CELL HOST & MICROBE

Toxoplasma gondii develops a latent infection in the muscle and central nervous system that acts as a reservoir for acute-stage re..

Pre-existing Toxoplasma gondii infection increases susceptibility to pentylenetetrazol-induced seizures independent of traumatic brain injury in mice.

Tamara L Baker, Alessandro D Uboldi, Christopher J Tonkin, David K Wright, Anh Vo, Trevor Wilson, Richelle Mychasiuk, Stuart J McDonald, Bridgette D Semple, Mujun Sun, Sandy R Shultz

Journal article  |  2022  |  Front Mol Neurosci

INTRODUCTION: Post-traumatic epilepsy (PTE) is a debilitating chronic outcome of traumatic brain injury (TBI), and neuroinflammati..

Depletion of a Toxoplasma porin leads to defects in mitochondrial morphology and contacts with the endoplasmic reticulum

Natalia Mallo, Jana Ovciarikova, Erica S Martins-Duarte, Stephan C Baehr, Marco Biddau, Mary-Louise Wilde, Alessandro D Uboldi, Leandro Lemgruber, Christopher J Tonkin, Jeremy G Wideman, Clare R Harding, Lilach Sheiner

Journal article  |  2021  |  JOURNAL OF CELL SCIENCE

The voltage-dependent anion channel (VDAC) is a ubiquitous channel in the outer membrane of the mitochondrion with multiple roles ..

Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT

Shengbo Zhang, Hannah D Coughlan, Mitra Ashayeripanah, Simona Seizova, Andrew J Kueh, Daniel Brown, Wang Cao, Nicolas Jacquelot, Angela D'Amico, Andrew M Lew, Yifan Zhan, Christopher J Tonkin, Jose A Villadangos, Gordon K Smyth, Michael Chopin, Stephen L Nutt

Journal article  |  2021  |  SCIENCE IMMUNOLOGY

The functional diversification of dendritic cells (DCs) is a key step in establishing protective immune responses. Despite the imp..

Direct Nanopore Sequencing of mRNA Reveals Landscape of Transcript Isoforms in Apicomplexan Parasites.

V Vern Lee, Louise M Judd, Aaron R Jex, Kathryn E Holt, Christopher J Tonkin, Stuart A Ralph

Journal article  |  2021  |  mSystems

Alternative splicing is a widespread phenomenon in metazoans by which single genes are able to produce multiple isoforms of the ge..

Environmental sensing and regulation of motility in Toxoplasma

Alessandro D Uboldi, Mary-Louise Wilde, Stefanie M Bader, Christopher J Tonkin

Journal article  |  2020  |  MOLECULAR MICROBIOLOGY

Toxoplasma and other apicomplexan parasites undergo a unique form of cellular locomotion referred to as "gliding motility." Glidin..