Danny Hatters runs a program exploring how proteins change conformation in cells as part of normal and pathogenic processes, including those associated with misfolding and aggregation. Key elements of this research include the development and application of new fluorescence -based probes and biosensors to view protein dynamics in cells.
His major area of research is in Huntington's Disease with other programs in motor neuron disease and fundamental biology of protein-ligand associations as part of cellular mechanisms. He completed his PhD at the University of Melbourne in 2002. He then did a post doc at the Gladstone Institutes/University of California, San Francisco under the mentorship of Dr Karl Weisgraber for 5 years until 2007. There he studied how three variants of apolipoprotein E, apoE2, apoE3 and apoE4 differ in their conformation and biophysical properties as a basis for understanding the mechanisms underlying the elevated risk that the apoE4 isoform confers for Alzheimer's disease. In April 2007, he returned to Melbourne to take up a CR Roper Fellowship position in the Department of Biochemistry and Molecular Biology. In 2009 he was awarded the Grimwade Fellowship to continue developing his own research program focusing on how protein conformations lead to cellular dysfunction and disease. In 2012, he was awarded an ARC Future Fellowship. In 2019 he was awarded an NHMRC Senior Research Fellowship.
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The Cascade Of Consequences In Huntington Disease From Mutant Httex1 Synthesis And Aggrega..
Misfolded Polyglutamine, Polyalanine, and Superoxide Dismutase 1 Aggregate via Distinct Pa..
Displaying the 22 most recent projects by Danny Hatters.
Internal Research Grant
Displaying the 81 most recent scholarly works by Danny Hatters.
Widespread remodeling of proteome solubility in response to different protein homeostasis stresses.
Xiaojing Sui, Douglas EV Pires, Angelique R Ormsby, Dezerae Cox, Shuai Nie, Giulia Vecchi, Michele Vendruscolo, David B Ascher, Gavin E Reid, Danny M Hatters
Journal article | 2020 | Proceedings of the National Academy of Sciences
The accumulation of protein deposits in neurodegenerative diseases has been hypothesized to depend on a metastable subproteome vul..
Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal
Lorena Hidalgo San Jose, Mary Jean Sunshine, Christopher H Dillingham, Bernadette A Chua, Miriama Kruta, Yuning Hong, Danny M Hatters, Robert AJ Signer
Journal article | 2020 | Cell Reports
Low protein synthesis is a feature of somatic stem cells that promotes regeneration in multiple tissues. Modest increases in prote..
Arginine valency in C9ORF72 dipolypeptides mediates promiscuous proteome binding that stalls ribosomes, disable actin cytoskeleton assembly and impairs arginine methylation of endogenous proteins
Mona Radwan, Ching-Seng Ang, Angelique Ormsby, Dezerae Cox, James Daly, Gavin Reid, Danny Hatters
Journal article | 2019
ABSTRACT C9ORF72 -associated Motor Neuron Disease patients feature abnormal expression of 5 dipeptide repeat (DPR) polymers. Here ..
Widespread remodelling of proteome solubility in response to different protein homeostasis stresses
Xiaojing Sui, Douglas Pires, Shuai Nie, Giulia Vecchi, Michele Vendruscolo, David Ascher, Gavin Reid, Danny Hatters
Journal article | 2019
SUMMARY The accumulation of wide range of different protein deposits in neurodegenerative diseases has been associated with the pr..
Protein aggregation in cell biology: An aggregomics perspective of health and disease.
Dezerae Cox, Candice Raeburn, Xiaojing Sui, Danny M Hatters
Journal article | 2018 | Seminars in Cell & Developmental Biology
Maintaining protein homeostasis (proteostasis) is essential for cellular health and is governed by a network of quality control ma..
Tadpole-like Conformations of Huntingtin Exon 1 Are Characterized by Conformational Heterogeneity that Persists regardless of Polyglutamine Length
Estella A Newcombe, Kiersten M Ruff, Ashish Sethi, Angelique R Ormsby, Yasmin M Ramdzan, Archa Fox, Anthony W Purcell, Paul R Gooley, Rohit Pappu, Danny M Hatters
Journal article | 2018 | Journal of Molecular Biology
A thiol probe for measuring unfolded protein load and proteostasis in cells
Moore Z Chen, Nagaraj S Moily, Jessica L Bridgford, Rebecca J Wood, Mona Radwan, Trevor A Smith, Zhegang Song, Ben Zhong Tang, Leann Tilley, Xiaohong Xu, Gavin E Reid, Mahmoud A Pouladi, Yuning Hong, Danny M Hatters
Journal article | 2017 | Nature communications
Transcriptional profiles for distinct aggregation states of mutant Huntingtin exon 1 protein unmask new Huntington's disease pathways
Nagaraj S Moily, Angelique R Ormsby, Aleksandar Stojilovic, Yasmin M Ramdzan, Jeannine Diesch, Ross D Hannan, Michelle S Zajac, Anthony J Hannan, Alicia Oshlack, Danny M Hatters
Journal article | 2017 | Molecular and Cellular Neuroscience
Tadpole-like conformations of huntingtin exon 1 with expanded polyglutamine engenders novel interactions in cells
Estella Newcombe, Kiersten Ruff, Ashish Sethi, Angelique Ormsby, Yasmin Ramdzan, Archa Fox, Anthony Purcell, Paul Gooley, Rohit Pappu, Danny Hatters
Journal article | 2017
ABSTRACT Soluble huntingtin exon 1 (Httex1) with expanded polyglutamine (polyQ) engenders neurotoxicity in Huntington’s disease. T..
Conformational dynamics and self-association of intrinsically disordered Huntingtin exon 1 in cells
Steffen Buening, Abhishek Sharma, Shivang Vachharajani, Estella Newcombe, Angelique Ormsby, Mimi Gao, David Gnutt, Tobias Voepel, Danny M Hatters, Simon Ebbinghaus
Journal article | 2017 | Physical Chemistry Chemical Physics
Huntingtin Inclusions Trigger Cellular Quiescence, Deactivate Apoptosis, and Lead to Delayed Necrosis
Yasmin M Ramdzan, Mikhail M Trubetskov, Angelique R Ormsby, Estella A Newcombe, Xiaojing Sui, Mark J Tobin, Marie N Bongiovanni, Sally L Gras, Grant Dewson, Jason ML Miller, Steven Finkbeiner, Nagaraj S Moily, Jonathan Niclis, Clare L Parish, Anthony W Purcell, Michael J Baker, Jacqueline A Wilce, Saboora Waris, Diana Stojanovski, Till Bocking, et al.
Journal article | 2017 | Cell reports
Polyalanine expansions drive a shift into alpha-helical clusters without amyloid-fibril formation
Saskia Polling, Angelique R Ormsby, Rebecca J Wood, Kristie Lee, Cheryl Shoubridge, James N Hughes, Paul Q Thomas, Michael DW Griffin, Andrew F Hill, Quill Bowden, Till Boecking, Danny M Hatters
Journal article | 2015 | Nature Structural & Molecular Biology
SOD1 protein aggregates stimulate macropinocytosis in neurons to facilitate their propagation
Rafaa Zeineddine, Jay F Pundavela, Lisa Corcoran, Elise M Stewart, Dzung Do-Ha, Monique Bax, Gilles Guillemin, Kara L Vine, Danny M Hatters, Heath Ecroyd, Christopher M Dobson, Bradley J Turner, Lezanne Ooi, Mark R Wilson, Neil R Cashman, Justin J Yerbury
Journal article | 2015 | Molecular Neurodegeneration
Prion-like domains in RNA binding proteins are essential for building subnuclear paraspeckles
Sven Hennig, Geraldine Kong, Taro Mannen, Agata Sadowska, Simon Kobelke, Amanda Blythe, Gavin J Knote, K Swaminathan Iyer, Diwei Ho, Estella A Newcombe, Kana Hosoki, Naoki Goshima, Tetsuya Kawaguchi, Danny Hatters, Laura Trinkle-Mulcahy, Tetsuro Hirose, Charles S Bond, Archa H Fox
Journal article | 2015 | The Journal of Cell Biology
SerpinB2 (PAI-2) Modulates Proteostasis via Binding Misfolded Proteins and Promotion of Cytoprotective Inclusion Formation
Jodi A Lee, Justin J Yerbury, Natalie Farrawell, Robert F Shearer, Patrick Constantinescu, Danny M Hatters, Wayne A Schroder, Andreas Suhrbier, Mark R Wilson, Darren N Saunders, Marie Ranson
Journal article | 2015 | PLOS ONE
Application of flow cytometry to analyze intracellular location and trafficking of cargo in cell populations
WH Toh, FJ Houghton, CP Chia, YM Ramdzan, DM Hatters, PA Gleeson
Book Chapter | 2015
© Springer Science+Business Media New York 2015. All rights are reserved.Pulse shape analysis (PulSA) is a flow cytometry-based me..
Application of flow cytometry to analyze intracellular location and trafficking of cargo in cell populations.
Wei Hong Toh, Fiona J Houghton, Pei Zhi Cheryl Chia, Yasmin M Ramdzan, Danny M Hatters, Paul A Gleeson
Journal article | 2015 | Methods in Molecular Biology
Pulse shape analysis (PulSA) is a flow cytometry-based method that involves the measurement of the pulse width and height of a flu..
Pulse shape analysis (PulSA) to track protein translocalization in cells by flow cytometry: applications for polyglutamine aggregation.
Yasmin M Ramdzan, Rebecca Wood, Danny M Hatters
Journal article | 2013 | Methods in Molecular Biology
Pulse shape analysis (PulSA) is a flow cytometry-based method that can be used to study protein localization patterns in cells. Ex..
Size analysis of polyglutamine protein aggregates using fluorescence detection in an analytical ultracentrifuge.
Saskia Polling, Danny M Hatters, Yee-Foong Mok
Journal article | 2013 | Methods in Molecular Biology
Defining the aggregation process of proteins formed by poly-amino acid repeats in cells remains a challenging task due to a lack o..
The Anti-Cancer IgM Monoclonal Antibody PAT-SM6 Binds with High Avidity to the Unfolded Protein Response Regulator GRP78
Zachary Rosenes, Terrence D Mulhern, Danny M Hatters, Leodevico L Ilag, Barbara E Power, Chris Hosking, Frank Hensel, Geoffrey J Howlett, Yee-Foong Mok
Journal article | 2012 | PLoS One
Disease-Associated Polyglutamine Stretches in Monomeric Huntingtin Adopt a Compact Structure
Clare Peters-Libeu, Jason Miller, Earl Rutenber, Yvonne Newhouse, Preethi Krishnan, Kenneth Cheung, Danny Hatters, Elizabeth Brooks, Kartika Widjaja, Tran Tina, Siddhartha Mitra, Montserrat Arrasate, Luis A Mosquera, Dean Taylor, Karl H Weisgraber, Steven Finkbeiner
Journal article | 2012 | Journal of Molecular Biology
Tracking protein aggregation and mislocalization in cells with flow cytometry
Yasmin M Ramdzan, Saskia Polling, Cheryl PZ Chia, Ivan HW Ng, Angelique R Ormsby, Nathan P Croft, Anthony W Purcell, Marie A Bogoyevitch, Dominic CH Ng, Paul A Gleeson, Danny M Hatters
Journal article | 2012 | Nature Methods
AMP-Activated Protein Kinase beta-Subunit Requires Internal Motion for Optimal Carbohydrate Binding
Michael Bieri, Jesse I Mobbs, Ann Koay, Gavin Louey, Yee-Foong Mok, Danny M Hatters, Jong-Tae Park, Kwan-Hwa Park, Dietbert Neumann, David Stapleton, Paul R Gooley
Journal article | 2012 | Biophysical Journal
Identifying polyglutamine protein species in situ that best predict neurodegeneration
Jason Miller, Montserrat Arrasate, Elizabeth Brooks, Clare Peters Libeu, Justin Legleiter, Danny Hatters, Jessica Curtis, Kenneth Cheung, Preethi Krishnan, Siddhartha Mitra, Kartika Widjaja, Benjamin A Shaby, Gregor P Lotz, Yvonne Newhouse, Emily J Mitchell, Alex Osmand, Michelle Gray, Vanitha Thulasiramin, Frederic Saudou, Mark Segal, et al.
Journal article | 2011 | Nature Chemical Biology
Structural and Functional Analysis of a Plant Resistance Protein TIR Domain Reveals Interfaces for Self-Association, Signaling, and Autoregulation
Maud Bernoux, Thomas Ve, Simon Williams, Christopher Warren, Danny Hatters, Eugene Valkov, Xiaoxiao Zhang, Jeffrey G Ellis, Bostjan Kobe, Peter N Dodds
Journal article | 2011 | Cell Host & Microbe
VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation
Sarada D Tetali, Madhu S Budamagunta, Catalina Simion, Laura J den Hartigh, Tamas Kalai, Kalman Hideg, Danny M Hatters, Karl H Weisgraber, John C Voss, John C Rutledge
Journal article | 2010 | Journal of Lipid Research
Honours, Awards and Fellowships
McAulay-Hope Prize for Original Biophysics
Applied Biosystems Edman Award
Biochemistry And Molecular Biology
Secretary Of Meeting
Lorne Conference on Protein Structure and Function
University of Melbourne
Bachelor of science (Hons)
University of Melbourne