James Wiley

Flow Cytometry Identifies an Early Stage of Platelet Apoptosis Produced by Agonists of the P2X1 and P2X7 Receptors

Joelyn Wong, Ben J Gu, Harry Teoh, Malgorzata Krupa, Mastura Monif, Mark Slee, James S Wiley

Journal article  |  2022  |  PLATELETS

Platelets express P2X1 receptors and our data also show the expression of P2X7 receptors. We studied the role of both receptors in..

Genomics of Alzheimer's disease implicates the innate and adaptive immune systems

Yihan Li, Simon M Laws, Luke A Miles, James S Wiley, Xin Huang, Colin L Masters, Ben J Gu

Journal article  |  2021  |  CELLULAR AND MOLECULAR LIFE SCIENCES

Alzheimer's disease (AD) is a chronic neurodegenerative disease characterised by cognitive impairment, behavioural alteration, and..

Deficits in Monocyte Function in Age Related Macular Degeneration: A Novel Systemic Change Associated With the Disease

Ben J Gu, Xin Huang, Pavan K Avula, Emily Caruso, Candace Drysdale, Kirstan A Vessey, Amber Ou, Christopher Fowler, Tian-Hua Liu, Yong Lin, Adam Horton, Colin L Masters, James S Wiley, Robyn H Guymer, Erica L Fletcher

Journal article  |  2021  |  FRONTIERS IN MEDICINE

Age-related macular degeneration (AMD) is characterized by the accumulation of debris in the posterior eye. In this study we evalu..

Regulation of the Acute Sickness Response by the P2RX7 Receptor

Hui Li, Erin Cvejic, Ben Gu, Ute Vollmer-Conna, Ian Hickie, Denis Wakefield, Tracey Davenport, James Wiley, Andrew R Lloyd

Journal article  |  2021  |  JOURNAL OF INFECTIOUS DISEASES

BACKGROUND: The acute sickness response to infection is a stereotyped set of illness manifestations initiated by proinflammatory s..

A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function

Kristen K Skarratt, Ben J Gu, Michael D Lovelace, Carol J Milligan, Leanne Stokes, Rachel Glover, Steven Petrou, James S Wiley, Stephen J Fuller

Journal article  |  2020  |  FASEB JOURNAL

P2X7 is an ATP-gated membrane ion channel that is expressed by multiple cell types. Brief exposure to ATP induces the opening of a..

Assays to Measure Purinoceptor Pore Dilation

Ben J Gu, Pavan Avula, James S Wiley

Journal article  |  2020  |  PURINERGIC SIGNALING: METHODS AND PROTOCOLS

The P2X7 receptor is a classic purinoceptor/ion channel. After activated by ATP, it opens a cation selective channel, which dilate..

Multiple sclerosis genomic map implicates peripheral immune cells and microglia in susceptibility

Nikolas A Patsopoulos, Sergio E Baranzini, Adams Santaniello, Parisa Shoostari, Chris Cotsapas, Garrett Wong, Ashley H Beecham, Tojo James, Joseph Replogle, Ioannis S Vlachos, Cristin McCabe, Tune H Pers, Aaron Brandes, Charles White, Brendan Keenan, Maria Cimpean, Phoebe Winn, Ioannis-Pavlos Panteliadis, Allison Robbins, Till FM Andlauer, et al.

Journal article  |  2019  |  SCIENCE

We analyzed genetic data of 47,429 multiple sclerosis (MS) and 68,374 control subjects and established a reference map of the gene..

P2X7 as a scavenger receptor for innate phagocytosis in the brain

Ben J Gu, James S Wiley

Journal article  |  2018  |  BRITISH JOURNAL OF PHARMACOLOGY

The P2X7 receptor has been widely studied for its ATP-induced pro-inflammatory effect, but in the absence of a ligand, P2X7 has a ..

The scavenger activity of the human P2X7 receptor differs from P2X7 pore function by insensitivity to antagonists, genetic variation and sodium concentration: Relevance to inflammatory brain diseases

Amber Ou, Ben J Gu, James S Wiley

Journal article  |  2018  |  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Activation of P2X7 receptors is widely recognised to initiate proinflammatory responses. However P2X7 also has a dual function as ..

Loss of Function of P2X7 Receptor Scavenger Activity in Aging Mice A Novel Model for Investigating the Early Pathogenesis of Age-Related Macular Degeneration

Kirstan A Vessey, Ben J Gu, Andrew I Jobling, Joanna A Phipps, Ursula Greferath, Mai X Tran, Michael A Dixon, Paul N Baird, Robyn H Guymer, James S Wiley, Erica L Fletcher

Journal article  |  2017  |  AMERICAN JOURNAL OF PATHOLOGY

Age-related macular degeneration (AMD) is a leading cause of irreversible, severe vision loss in Western countries. Recently, we i..