Andrew Elefanty

Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells.

Monica Nafria, Constanze Bonifer, Edouard Guy Stanley, Elizabeth Siewsun Ng, Andrew George Elefanty

Journal article  |  2020  |  STAR Protocols

This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent ..

Human yolk sac-like haematopoiesis generates RUNX1-, GFI1- and/or GFI1B-dependent blood and SOX17-positive endothelium

Freya F Bruveris, Elizabeth S Ng, Ana Rita Leitoguinho, Ali Motazedian, Katerina Vlahos, Koula Sourris, Robyn Mayberry, Penelope McDonald, Lisa Azzola, Nadia M Davidson, Alicia Oshlack, Edouard G Stanley, Andrew G Elefanty

Journal article  |  2020  |  Development (Cambridge)

The genetic regulatory network controlling early fate choices during human blood cell development are not well understood. We used..

CRISPR/Cas9 gene editing of a SOX9 reporter human iPSC line to produce two TRPV4 patient heterozygous missense mutant iPSC lines, MCRIi001-A-3 (TRPV4 p.F273L) and MCRIi001-A-4 (TRPV4 p.P799L)

Yudha Nur Patria, Tayla Stenta, Jinia Lilianty, Lynn Rowley, Edouard G Stanley, Andrew G Elefanty, John F Bateman, Shireen R Lamande

Journal article  |  2020  |  Stem Cell Research

To produce in vitro models of human chondrodysplasias caused by dominant missense mutations in TRPV4, we used CRISPR/Cas9 gene edi..

Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells

Monica Nafria, Peter Keane, Elizabeth S Ng, Edouard G Stanley, Andrew G Elefanty, Constanze Bonifer

Journal article  |  2020  |  Cell Reports

Acute myeloid leukemia (AML) is a hematopoietic malignancy caused by recurrent mutations in genes encoding transcriptional, chroma..

Generation of a heterozygous COL2A1 (p.R989C) spondyloepiphyseal dysplasia congenita mutation iPSC line, MCRIi001-B, using CRISPR/Cas9 gene editing

Jinia Lilianty, Yudha Nur Patria, Edouard G Stanley, Andrew G Elefanty, John F Bateman, Shireen R Lamande

Journal article  |  2020  |  Stem Cell Research

To produce an in vitro model of the human chondrodysplasia, spondyloepiphyseal dysplasia congenita, we used CRISPR/Cas9 gene editi..

Multipotent RAG1 progenitors emerge directly from haemogenic endothelium in human pluripotent stem cell-derived haematopoietic organoids

Ali Motazedian, Freya F Bruveris, Santhosh V Kumar, Jacqueline V Schiesser, Tyrone Chen, Elizabeth S Ng, Ann P Chidgey, Christine A Wells, Andrew G Elefanty, Edouard G Stanley

Journal article  |  2020  |  Nature Cell Biology

Defining the ontogeny of the human adaptive immune system during embryogenesis has implications for understanding childhood diseas..

Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing

Yudha Nur Patria, Jinia Lilianty, Andrew G Elefanty, Edouard G Stanley, Tanya Labonne, John F Bateman, Shireen R Lamande

Journal article  |  2020  |  Stem Cell Research

To develop an iPSC SOX9 reporter line for monitoring differentiation into SOX9 expressing cells such as chondrocytes, cranial neur..

Induced pluripotent stem cell macrophages present antigen to proinsulin-specific T cell receptors from donor-matched islet-infiltrating T cells in type 1 diabetes

Kriti Joshi, Colleen Elso, Ali Motazedian, Tanya Labonne, Jacqueline V Schiesser, Fergus Cameron, Stuart I Mannering, Andrew G Elefanty, Edouard G Stanley

Journal article  |  2019  |  Diabetologia

AIMS/HYPOTHESIS: Type 1 diabetes is an autoimmune disorder characterised by loss of insulin-producing beta cells of the pancreas. ..

Loss of Phospholipase C Gamma 1 (PLCG1) Impairs AML1-ETO Induced Leukemia Stem Cell Self-Renewal

Tina M Schnoeder, Adrian Schwarzer, Florian Perner, Joanna Kirkpatrick, Anna Dolnik, Konstanze Doehner, Patricia Arreba-Tutusaus, Salam A Assi, Claudia Bruedigam, Carolin Kathner, Theresa Eifert, Marcus Buschbeck, Monica Nafria I Fedi, Anetta Ptasinska, Andrew Elefanty, Elizabeth Ng, Holger Bierhoff, Florian Gras, Georg Matziolis, Klaus-Dieter Fischer, et al.

Conference Proceedings  |  2019  |  Blood

The role of cardiac transcription factor NKX2-5 in regulating the human cardiac miRNAome

Deevina Arasaratnam, Katrina M Bell, Choon Boon Sim, Kathy Koutsis, David J Anderson, Elizabeth L Qian, Edouard G Stanley, Andrew G Elefanty, Michael M Cheung, Alicia Oshlack, Anthony J White, Charbel Abi Khalil, James E Hudson, Enzo R Porrello, David A Elliott

Journal article  |  2019  |  Scientific Reports

MicroRNAs (miRNAs) are translational regulatory molecules with recognised roles in heart development and disease. Therefore, it is..