Little, MH

Returning to kidney development to deliver synthetic kidneys

Melissa H Little

Journal article  |  2021  |  Developmental Biology

There is no doubt that the development of transplantable synthetic kidneys could improve the outcome for the many millions of peop..

Autonomous Calcium Signaling in Human and Zebrafish Podocytes Controls Kidney Filtration Barrier Morphogenesis.

Lydia Djenoune, Ritu Tomar, Aude Dorison, Irene Ghobrial, Heiko Schenk, Jan Hegermann, Lynne Beverly-Staggs, Alejandro Hidalgo-Gonzalez, Melissa H Little, Iain A Drummond

Journal article  |  2021  |  Journal of the American Society of Nephrology

BACKGROUND: Podocytes are critical to maintaining the glomerular filtration barrier, and mutations in nephrotic syndrome genes are..

Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk

Sara E Howden, Sean B Wilson, Ella Groenewegen, Lakshi Starks, Thomas A Forbes, Ker Sin Tan, Jessica M Vanslambrouck, Emily M Holloway, Yi-Hsien Chen, Sanjay Jain, Jason R Spence, Melissa H Little

Journal article  |  2021  |  Cell Stem Cell

During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous..

Mentorship in Science: Response to AlShebli et al., Nature Communications 2020

Christine Mummery, Melissa Little, Haifan Lin, Amander Clark, Ken Zaret, Deepak Srivastava, Elaine Fuchs, Fiona Watt, Sally Temple

Journal article  |  2021  |  Stem Cell Reports

Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice

Amar J Majmundar, Florian Buerger, Thomas A Forbes, Verena Klambt, Ronen Schneider, Konstantin Deutsch, Thomas M Kitzler, Sara E Howden, Michelle Scurr, Ker Sin Tan, Mickael Krzeminski, Eugen Widmeier, Daniela A Braun, Ethan Lai, Ihsan Ullah, Ali Amar, Amy Kolb, Kaitlyn Eddy, Chin Heng Chen, Daanya Salmanullah, et al.

Journal article  |  2021  |  Science Advances

Nephrotic syndrome (NS) is a leading cause of chronic kidney disease. We found recessive NOS1AP variants in two families with earl..

An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development

Ingrid M Knarston, Svenja Pachernegg, Gorjana Robevska, Irene Ghobrial, Pei Xuan Er, Elizabeth Georges, Minoru Takasato, Alexander N Combes, Anne Jorgensen, Melissa H Little, Andrew H Sinclair, Katie L Ayers

Journal article  |  2020  |  Stem Cell Reports

Currently an in vitro model that fully recapitulates the human embryonic gonad is lacking. Here we describe a fully defined feeder..

Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation

Kynan T Lawlor, Jessica M Vanslambrouck, J William Higgins, Alison Chambon, Kristina Bishard, Derek Arndt, Pei Xuan Er, Sean B Wilson, Sara E Howden, Ker Sin Tan, Fanyi Li, Lorna J Hale, Benjamin Shepherd, Stephen Pentoney, Sharon C Presnell, Alice E Chen, Melissa H Little

Journal article  |  2020  |  Nature Materials

Directed differentiation of human pluripotent stem cells to kidney organoids brings the prospect of drug screening, disease modell..

Patterning a Ureter Is All in the Stroma

Melissa H Little

Journal article  |  2020  |  Journal of the American Society of Nephrology

Advances in our understanding of genetic kidney disease using kidney organoids

Melissa H Little, Catherine Quinlan

Journal article  |  2020  |  Pediatric Nephrology

A significant proportion of kidney disease presenting in childhood is likely genetic in origin with a growing number of genes impl..

Engineering Tissues and Organs: The Road to the Clinic

Melissa Little, Guang-Hui Liu, Krishna V Shenoy, Gordana Vunjak-Novakovic, Milica Radisic

Journal article  |  2020  |  Cell

With recent advances in both gene editing and stem cell biology, the promise of cellular therapies is now closer than ever. Clinic..