Little, MH

Kidney Organoids Generated Using an Allelic Series of NPHS2 Point Variants Reveal Distinct Intracellular Podocin Mistrafficking

Aude Dorison, Irene Ghobrial, Alison Graham, Thanushi Peiris, Thomas AA Forbes, Michael See, Mithun Das, Moin AA Saleem, Catherine Quinlan, Kynan TT Lawlor, Mirana Ramialison, Sara EE Howden, Melissa HH Little

Journal article  |  2023  |  JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

BACKGROUND: NPHS2 variants are the most common cause of steroid-resistant nephrotic syndrome in children >1 month old. Missense NP..

What can we learn from kidney organoids?

Aude Dorison, Thomas A Forbes, Melissa H Little

Journal article  |  2022  |  KIDNEY INTERNATIONAL

The ability to generate 3-dimensional models of the developing human kidney via the directed differentiation of pluripotent stem c..

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

Jessica M Vanslambrouck, Sean B Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil, Kynan T Lawlor, Sophia Mah, Michelle Scurr, Sara E Howden, Kanta Subbarao, Melissa H Little

Journal article  |  2022  |  NATURE COMMUNICATIONS

While pluripotent stem cell-derived kidney organoids are now being used to model renal disease, the proximal nephron remains immat..

The BCL-2 family member BID plays a role during embryonic development in addition to its BH3-only protein function by acting in parallel to BAX, BAK and BOK

Francine S Ke, Steven Holloway, Rachel T Uren, Agnes W Wong, Melissa H Little, Ruth M Kluck, Anne K Voss, Andreas Strasser

Journal article  |  2022  |  EMBO JOURNAL

The intrinsic apoptosis pathway, regulated by the BCL-2 protein family, is essential for embryonic development. Using mice lacking..

2021-2022: A year in review

Melissa Little

Journal article  |  2022  |  STEM CELL REPORTS

ISSCR Presidents look back on their presidency, the evolution of the field, and the Society

Leonard Zon, Gordon Keller, George Q Daley, Fiona M Watt, Irving L Weissman, Elaine Fuchs, Fred H Gage, Shinya Yamanaka, Janet Rossant, Sean Morrison, Sally Temple, Hans C Clevers, Deepak Srivastava, Christine L Mummery, Melissa Little

Journal article  |  2022  |  STEM CELL REPORTS

In celebration of the ISSCR's 20th anniversary we asked past ISSCR presidents the question, "During your presidential year, what k..

Anniversary reflections: ISSCR presidents on 20 years of progress

Melissa Little, Sean J Morrison, Janet Rossant, Deepak Srivastava, George Q Daley

Journal article  |  2022  |  CELL STEM CELL

This year, Cell Stem Cell and the International Society for Stem Cell Research (ISSCR) are celebrating their 15th and 20th anniver..

DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets

Sean B Wilson, Sara E Howden, Jessica M Vanslambrouck, Aude Dorison, Jose Alquicira-Hernandez, Joseph E Powell, Melissa H Little

Journal article  |  2022  |  GENOME MEDICINE

BACKGROUND: While single-cell transcriptional profiling has greatly increased our capacity to interrogate biology, accurate cell c..

Forward steps in organoid-based forward screening

Melissa H Little, Sara E Howden

Journal article  |  2022  |  CELL STEM CELL

In this issue of Cell Stem Cell, Ungricht et al. (2022) perform a temporally controlled CRISPR/Cas9-based genome-wide screen in ki..

Regrow or Repair: An Update on Potential Regenerative Therapies for the Kidney

Melissa H Little, Benjamin D Humphreys

Journal article  |  2022  |  JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Fifteen years ago, this journal published a review outlining future options for regenerating the kidney. At that time, stem cell p..