Sandy Hung

Comparison of CRISPR/Cas Endonucleases forin vivoRetinal Gene Editing

Fan Li, Kristof Wing, Jiang-Hui Wang, Chi D Luu, James A Bender, Jinying Chen, Qi Wang, Qinyi Lu, Minh Thuan Nguyen Tran, Kaylene M Young, Raymond CB Wong, Alice Pebay, Anthony L Cook, Sandy SC Hung, Guei-Sheung Liu, Alex W Hewitt

Journal article  |  2020  |  FRONTIERS IN CELLULAR NEUROSCIENCE

CRISPR/Cas has opened the prospect of direct gene correction therapy for some inherited retinal diseases. Previous work has demons..

Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

Anne Senabouth, Stacey Andersen, Qianyu Shi, Lei Shi, Feng Jiang, Wenwei Zhang, Kristof Wing, Maciej Daniszewski, Samuel W Lukowski, Sandy SC Hung, Quan Nguyen, Lynn Fink, Anthony Beckhouse, Alice Pebay, Alex W Hewitt, Joseph E Powell

Journal article  |  2020  |  NAR GENOMICS AND BIOINFORMATICS

The libraries generated by high-throughput single cell RNA-sequencing (scRNA-seq) platforms such as the Chromium from 10× Genomics..

Towards retinal regeneration: Reprogramming retinal glial cells into photoreceptors using CRISPRa

Raymond Wong, Tu Nguyen, Lyujie Fang, Sam Lukowski, Camden Lo, Sandy Hung, Chi D Luu, Mark C Gillies, Alex W Hewitt

Conference Proceedings  |  2020  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Utility of Self-Destructing CRISPR/Cas Constructs for Targeted Gene Editing in the Retina

Fan Li, Sandy SC Hung, Mohd Khairul Nizam Mohd Khalid, Jiang-Hui Wang, Vicki Chrysostomou, Vickie HY Wong, Vikrant Singh, Kristof Wing, Leilei Tu, James A Bender, Alice Pebay, Anna E King, Anthony L Cook, Raymond CB Wong, Bang Bui, Alex W Hewitt, Guei-Sheung Liu

Journal article  |  2019  |  HUMAN GENE THERAPY

Safe delivery of CRISPR/Cas endonucleases remains one of the major barriers to the widespread application of in vivo genome editin..

A single-cell transcriptome atlas of the adult human retina

Samuel W Lukowski, Camden Y Lo, Alexei A Sharov, Quan Nguyen, Lyujie Fang, Sandy SC Hung, Ling Zhu, Ting Zhang, Ulrike Grunert, Tu Nguyen, Anne Senabouth, Jafar S Jabbari, Emily Welby, Jane C Sowden, Hayley S Waugh, Adrienne Mackey, Graeme Pollock, Trevor D Lamb, Peng-Yuan Wang, Alex W Hewitt, et al.

Journal article  |  2019  |  EMBO JOURNAL

The retina is a specialized neural tissue that senses light and initiates image processing. Although the functional organization o..

Intervention Fidelity Monitoring for the Post Ischaemic Stroke Cardiovascular Exercise Study (PISCES)

Stanley Hung, Sharon Kramer, Emilio Werden, Liam Johnson, Rosalind Hutchings, Laura Bird, Julie Bernhardt, Amy Brodtmann

Conference Proceedings  |  2019  |  INTERNATIONAL JOURNAL OF STROKE

CRISPR/Cas-mediated base editing of the AMD high-risk Y402H complement factor H variant

Alex W Hewitt, Peter Tran, Mohd Khairul Nizam Mohd Khalid, Alice Pebay, Anthony L Cook, Helena Liang, Raymond Wong, Jamie E Craig, Guei-Sheung Liu, Sandy Hung

Journal article  |  2019  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Comparison of CRISPR/Cas endonuclease gene editing efficiency of retinal cells in vivo

Fan Li, Kristof Wing, Jiang-Hui Wang, James Bender, Chi D Luu, Jinying Chen, Vivienne Lu, Qi Wang, Qinyi Lu, Peter Tran, Kaylene Young, Anna King, Sandy Hung, Guei-Sheung Liu, Alex W Hewitt

Conference Proceedings  |  2019  |  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant

Thuan Nguyen Tran Minh, Mohd Khairul Nizam Mohd Khalid, Alice Pebay, Anthony L Cook, Helena H Liang, Raymond CB Wong, Jamie E Craig, Guei-Sheung Liu, Sandy S Hung, Alex W Hewitt

Journal article  |  2019  |  MOLECULAR VISION

Purpose: To evaluate the efficacy of using a CRISPR/Cas-mediated strategy to correct a common high-risk allele that is associated ..

A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9

Lyujie Fang, Sandy SC Hung, Jennifer Yek, Layal El Wazan, Nguyen Tu, Shahnaz Khan, Shiang Y Lim, Alex W Hewitt, Raymond CB Wong

Journal article  |  2019  |  MOLECULAR THERAPY-NUCLEIC ACIDS

Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond the physiologi..