Isabelle Lucet

Crystal structure of the putative cell-wall lipoglycan biosynthesis protein LmcA from Mycobacterium smegmatis.

Onisha Patel, Rajini Brammananth, Weiwen Dai, Santosh Panjikar, Ross L Coppel, Isabelle S Lucet, Paul K Crellin

Journal article  |  2022  |  Acta Crystallogr D Struct Biol

The bacterial genus Mycobacterium includes important pathogens, most notably M. tuberculosis, which infects one-quarter of the ent..

Distinct PEAK3 interactors and outputs expand the signaling potential of the PEAK pseudokinase family

Jianmei Hou, Elizabeth Nguyen, Minglyanna Surudoi, Michael J Roy, Onisha Patel, Isabelle S Lucet, Xiuquan Ma, Roger J Daly

Journal article  |  2022  |  SCIENCE SIGNALING

The pseudokinase scaffolds PEAK1 and PEAK2 are implicated in cancer cell migration and metastasis. We characterized the regulation..

Production and purification of the PEAK pseudokinases for structural and functional studies.

Onisha Patel, Minglyanna Surudoi, Weiwen Dai, Joshua M Hardy, Michael J Roy, Isabelle S Lucet

Book Chapter  |  2022  |  Methods in Enzymology

The PEAK family of pseudokinases, which comprises PEAK1, PEAK2 and PEAK3, are newly identified scaffolds that dynamically assemble..

Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1

Onisha Patel, Michael J Roy, Ashleigh Kropp, Joshua M Hardy, Weiwen Dai, Isabelle S Lucet

Journal article  |  2021  |  COMMUNICATIONS BIOLOGY

Doublecortin-like kinase 1 (DCLK1) is an understudied bi-functional kinase with a proven role in tumour growth and development. Ho..

The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs

Lung-Yu Liang, Michael Roy, Christopher R Horne, Jarrod J Sandow, Minglyanna Surudoi, Laura F Dagley, Samuel N Young, Toby Dite, Jeffrey J Babon, Peter W Janes, Onisha Patel, James M Murphy, Isabelle S Lucet

Journal article  |  2021  |  BIOCHEMICAL JOURNAL

EphB6 and EphA10 are two poorly characterised pseudokinase members of the Eph receptor family, which collectively serves as mediat..

Meeting reports: Kinases and pseudokinases online symposium

C Dodson, P Eyers, I Lucet, J Murphy

Journal article  |  2021  |  Biochemist

Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance

Laura Ramos Garcia, Tencho Tenev, Richard Newman, Rachel O Haich, Gianmaria Liccardi, Sidonie Wicky John, Alessandro Annibaldi, Lu Yu, Mercedes Pardo, Samuel N Young, Cheree Fitzgibbon, Winnie Fernando, Naomi Guppy, Hyojin Kim, Lung-Yu Liang, Isabelle S Lucet, Andrew Kueh, Ioannis Roxanis, Patrycja Gazinska, Martin Sims, et al.

Journal article  |  2021  |  NATURE COMMUNICATIONS

Necroptosis is a lytic, inflammatory form of cell death that not only contributes to pathogen clearance but can also lead to disea..

Granulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory alpha C helix

Michael R Oliver, Christopher R Horne, Safal Shrestha, Jeremy R Keown, Lung-Yu Liang, Samuel N Young, Jarrod J Sandow, Andrew Webb, David C Goldstone, Isabelle S Lucet, Natarajan Kannan, Peter Metcalf, James M Murphy

Journal article  |  2021  |  NATURE COMMUNICATIONS

The life cycle of Baculoviridae family insect viruses depends on the viral protein kinase, PK-1, to phosphorylate the regulatory p..

Development and application of a high-throughput screening assay for identification of small molecule inhibitors of the P. falciparum reticulocyte binding-like homologue 5 protein

Brad E Sleebs, Kate E Jarman, Sonja Frolich, Wilson Wong, Julie Healer, Weiwen Dai, Isabelle S Lucet, Danny W Wilson, Alan F Cowman

Journal article  |  2020  |  INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE

The P. falciparum parasite, responsible for the disease in humans known as malaria, must invade erythrocytes to provide an environ..

A regulatory region on RIPK2 is required for XIAP binding and NOD signaling activity

Valentin J Heim, Laura F Dagley, Che A Stafford, Fynn M Hansen, Elise Clayer, Aleksandra Bankovacki, Andrew Webb, Isabelle S Lucet, John Silke, Ueli Nachbur

Journal article  |  2020  |  EMBO REPORTS

Signaling via the intracellular pathogen receptors nucleotide-binding oligomerization domain-containing proteins NOD1 and NOD2 req..