Isabelle Lucet

Mapping and functional analysis of heterochromatin protein 1 phosphorylation in the malaria parasite Plasmodium falciparum

Hai TN Bui, Igor Niederwieser, Megan J Bird, Weiwen Dai, Nicolas MB Brancucci, Suzette Moes, Paul Jenoe, Isabelle S Lucet, Christian Doerig, Till S Voss

Journal article  |  2019  |  Scientific Reports

Previous studies in model eukaryotes have demonstrated that phosphorylation of heterochromatin protein 1 (HP1) is important for dy..

The PEAK family of pseudokinases, their role in cell signalling and cancer

Onisha Patel, Michael J Roy, James M Murphy, Isabelle S Lucet

Journal article  |  2019  |  The FEBS Journal

The study of pseudokinases has uncovered that catalysis-independent functions play a critical role in cell signalling regulation. ..

Eph receptor signalling: from catalytic to non-catalytic functions

Lung-Yu Liang, Onisha Patel, Peter W Janes, James M Murphy, Isabelle S Lucet

Journal article  |  2019  |  Oncogene

Viral MLKL Homologs Subvert Necroptotic Cell Death by Sequestering Cellular RIPK3

Emma J Petrie, Jarrod J Sandow, Wil IL Lehmann, Lung-Yu Liang, Diane Coursier, Samuel N Young, Wilhelmus JA Kersten, Cheree Fitzgibbon, Andre L Samson, Annette V Jacobsen, Kym N Lowes, Amanda E Au, Helene Jousset Sabroux, Najoua Lalaoui, Andrew I Webb, Guillaume Lessene, Gerard Manning, Isabelle S Lucet, James M Murphy

Journal article  |  2019  |  Cell Reports

A Structural Perspective of the Pseudokinome: Defining the Targetable Space

Isabelle S Lucet, James M Murphy

Book Chapter  |  2019  |  RSC Drug Discovery Series

CHAPTER 13: A Structural Perspective of the Pseudokinome: Defining the Targetable Space

IS Lucet, JM Murphy

Book Chapter  |  2019  |  RSC Drug Discovery Series

FSHD2-and BAMS-associated mutations confer opposing effects on SMCHD1 function

Alexandra D Gurzau, Kelan Chen, Shifeng Xue, Weiwen Dai, Isabelle S Lucet, Thao Nguyen Ly Thanh, Bruno Reversade, Marnie E Blewitt, James M Murphy

Journal article  |  2018  |  Journal of Biological Chemistry

Conformational switching of the pseudokinase domain promotes human MLKL tetramerization and cell death by necroptosis

Emma J Petrie, Jarrod J Sandowl, Annette Jacobsen, Brian J Smith, Michael DW Griffin, Isabelle S Lucet, Weiwen Dai, Samuel N Young, Maria C Tanzer, Ahmad Wardak, Lung-Yu Liang, Angus D Cowan, Joanne M Hildebrand, Wilhelmus JA Kersten, Guillaume Lessene, John Silke, Peter E Czabotar, Andrew Webb, James M Murphy

Journal article  |  2018  |  Nature Communications

The molecular basis of JAK/STAT inhibition by SOCS1

Nicholas PD Liau, Artem Laktyushin, Isabelle S Lucet, James M Murphy, Shenggen Yao, Eden Whitlock, Kimberley Callaghan, Nicos A Nicola, Nadia J Kershaw, Jeffrey J Babon

Journal article  |  2018  |  Nature Communications

Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association

Onisha Patel, Michael DW Griffin, Santosh Panjikar, Weiwen Dai, Xiuquan Ma, Howard Chan, Celine Zheng, Ashleigh Kropp, James M Murphy, Roger J Daly, Isabelle S Lucet

Journal article  |  2017  |  Nature Communications

Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay

Isabelle S Lucet, James M Murphy

Journal article  |  2017  |  Methods in molecular biology (Clifton, N.J.)

Homo- and Heterotypic Association Regulates Signaling by the SgK269/PEAK1 and SgK223 Pseudokinases

Ling Liu, Yu Wei Phua, Rachel S Lee, Xiuquan Ma, Yiping Jenkins, Karel Novy, Emily S Humphrey, Howard Chan, Robert Shearer, Poh Chee Ong, Weiwen Dai, Darren N Saunders, Isabelle S Lucet, Roger J Daly

Journal article  |  2016  |  Journal of Biological Chemistry

Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites

Boris Prinz, Katherine L Harvey, Louisa Wilcke, Ulrike Ruch, Klemens Engelberg, Laura Biller, Isabelle Lucet, Steffen Erkelenz, Dorothee Heincke, Tobias Spielmann, Christian Doerig, Conrad Kunick, Brendan S Crabb, Paul R Gilson, Tim W Gilberger

Journal article  |  2016  |  Scientific Reports

Biochemical and Structural Insights into Doublecortin-like Kinase Domain 1

Onisha Patel, Weiwen Dai, Mareike Mentzel, Michael DW Griffin, Juliette Serindoux, Yoann Gay, Stefanie Fischer, Shoukat Sterle, Ashleigh Kropp, Christopher J Burns, Matthias Ernst, Michael Buchert, Isabelle S Lucet

Journal article  |  2016  |  Structure

Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond.

Wesley C Van Voorhis, John H Adams, Roberto Adelfio, Vida Ahyong, Myles H Akabas, Pietro Alano, Aintzane Alday, Yesmalie Alemán Resto, Aishah Alsibaee, Ainhoa Alzualde, Katherine T Andrews, Simon V Avery, Vicky M Avery, Lawrence Ayong, Mark Baker, Stephen Baker, Choukri Ben Mamoun, Sangeeta Bhatia, Quentin Bickle, Lotfi Bounaadja, et al.

Journal article  |  2016  |  PLOS Pathogens

A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. M..

The epigenetic regulator Smchd1 contains a functional GHKL-type ATPase domain

Kelan Chen, Renwick CJ Dobson, Isabelle S Lucet, Samuel N Young, F Grant Pearce, Marnie E Blewitt, James M Murphy

Journal article  |  2016  |  Biochemical Journal

HSP90 activity is required for MLKL oligomerisation and membrane translocation and the induction of necroptotic cell death

AV Jacobsen, KN Lowes, MC Tanzer, IS Lucet, JM Hildebrand, EJ Petrie, MF van Delft, Z Liu, SA Conos, J-G Zhang, DCS Huang, J Silke, G Lessene, JM Murphy

Journal article  |  2016  |  Cell Death & Disease

Plasmodium falciparum Adhesins Play an Essential Role in Signalling and Activation of Invasion into Human Erythrocytes

Wai-Hong Tham, Nicholas TY Lim, Greta E Weiss, Sash Lopaticki, Brendan RE Ansell, Megan Bird, Isabelle Lucet, Dominique Dorin-Semblat, Christian Doerig, Paul R Gilson, Brendan S Crabb, Alan F Cowman

Journal article  |  2015  |  PLOS Pathogens

Molecular Mechanism of CCAAT-Enhancer Binding Protein Recruitment by the TRIB1 Pseudokinase

James M Murphy, Yoshio Nakatani, Sam A Jamieson, Weiwen Dai, Isabelle S Lucet, Peter D Mace

Journal article  |  2015  |  Structure

Genome-wide binding and mechanistic analyses of Smchd1-mediated epigenetic regulation

Kelan Chen, Jiang Hu, Darcy L Moore, Ruijie Liu, Sarah A Kessans, Kelsey Breslin, Isabelle S Lucet, Andrew Keniry, Huei San Leong, Clare L Parish, Douglas J Hilton, Richard JLF Lemmers, Silvere M van der Maarel, Peter E Czabotar, Renwick CJ Dobson, Matthew E Ritchie, Graham F Kay, James M Murphy, Marnie E Blewitt

Journal article  |  2015  |  Proceedings of the National Academy of Sciences

Flicking the molecular switch underlying MLKL-mediated necroptosis

Joanne M Hildebrand, Isabelle S Lucet, James M Murphy

Journal article  |  2015  |  Molecular & Cellular Oncology

An Extensive Antigenic Footprint Underpins Immunodominant TCR Adaptability against a Hypervariable Viral Determinant

Usha K Nivarthi, Stephanie Gras, Lars Kjer-Nielsen, Richard Berry, Isabelle S Lucet, John J Miles, Samantha L Tracy, Anthony W Purcell, David S Bowden, Margaret Hellard, Jamie Rossjohn, James McCluskey, Mandvi Bharadwaj

Journal article  |  2014  |  The Journal of Immunology

Activation of the pseudokinase MLKL unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death

Joanne M Hildebrand, Maria C Tanzer, Isabelle S Lucet, Samuel N Young, Sukhdeep K Spall, Pooja Sharma, Catia Pierotti, Jean-Marc Garnier, Renwick CJ Dobson, Andrew I Webb, Anne Tripaydonis, Jeffrey J Babon, Mark D Mulcair, Martin J Scanlon, Warren S Alexander, Andrew F Wilks, Peter E Czabotar, Guillaume Lessene, James M Murphy, John Silke

Journal article  |  2014  |  Proceedings of the National Academy of Sciences

The molecular regulation of Janus kinase (JAK) activation

Jeffrey J Babon, Isabelle S Lucet, James M Murphy, Nicos A Nicola, Leila N Varghese

Journal article  |  2014  |  Biochemical Journal

An exported kinase (FIKK4.2) that mediates virulence-associated changes in Plasmodium falciparum-infected red blood cells

Lev M Kats, Kate M Fernandez, Fiona K Glenister, Susann Herrmann, Donna W Buckingham, Ghizal Siddiqui, Laveena Sharma, Rebecca Bamert, Isabelle Lucet, Micheline Guillotte, Odile Mercereau-Puijalon, Brian M Cooke

Journal article  |  2014  |  International Journal for Parasitology

Collaborative Drug Design of Plasmodium Kinase Inhibitors

B Hardy, R Affentranger, A Contini, HG de Teran, J Spitzner, R Papoian, WL Seibel, S Nelson, J Wiseman, SD Bryant, I Lucet, C Doerig

Book Chapter  |  2013

The Pseudokinase MLKL Mediates Necroptosis via a Molecular Switch Mechanism

James M Murphy, Peter E Czabotar, Joanne M Hildebrand, Isabelle S Lucet, Jian-Guo Zhang, Silvia Alvarez-Diaz, Rowena Lewis, Najoua Lalaoui, Donald Metcalf, Andrew I Webb, Samuel N Young, Leila N Varghese, Gillian M Tannahill, Esme C Hatchell, Ian J Majewski, Toru Okamoto, Renwick CJ Dobson, Douglas J Hilton, Jeffrey J Babon, Nicos A Nicola, et al.

Journal article  |  2013  |  Immunity

Production and crystallization of recombinant JAK proteins.

Isabelle S Lucet, Rebecca Bamert

Journal article  |  2013  |  Methods in molecular biology (Clifton, N.J.)

JAK kinases are critical mediators in development, differentiation, and homeostasis and accordingly, have become well-validated ta..

Plasmodium kinases as targets for new-generation antimalarials.

Isabelle S Lucet, Andrew Tobin, David Drewry, Andrew F Wilks, Christian Doerig

Journal article  |  2012  |  Future Medicinal Chemistry

There is an urgent need for the development of new antimalarial drugs with novel modes of actions. The malarial parasite, Plasmodi..

Differential Recognition of CD1d-alpha-Galactosyl Ceramide by the V beta 8.2 and V beta 7 Semi-invariant NKT T Cell Receptors

Daniel G Pellicci, Onisha Patel, Lars Kjer-Nielsen, Siew Siew Pang, Lucy C Sullivan, Konstantinos Kyparissoudis, Andrew G Brooks, Hugh H Reid, Stephanie Gras, Isabelle S Lucet, Ruide Koh, Mark J Smyth, Thierry Mallevaey, Jennifer L Matsuda, Laurent Gapin, James McCluskey, Dale I Godfrey, Jamie Rossjohn

Journal article  |  2009  |  Immunity

Dissecting specificity in the Janus kinases: the structures of JAK-specific inhibitors complexed to the JAK1 and JAK2 protein tyrosine kinase domains.

Neal K Williams, Rebecca S Bamert, Onisha Patel, Christina Wang, Patricia M Walden, Andrew F Wilks, Emmanuelle Fantino, Jamie Rossjohn, Isabelle S Lucet

Journal article  |  2009  |  Journal of Molecular Biology

The Janus kinases (JAKs) are a pivotal family of protein tyrosine kinases (PTKs) that play prominent roles in numerous cytokine si..

Crystal structures of the Lyn protein tyrosine kinase domain in its Apo- and inhibitor-bound state.

Neal K Williams, Isabelle S Lucet, S Peter Klinken, Evan Ingley, Jamie Rossjohn

Journal article  |  2009  |  Journal of Biological Chemistry

The Src-family protein-tyrosine kinase (PTK) Lyn is the most important Src-family kinase in B cells, having both inhibitory and st..

The 2.7 A crystal structure of the autoinhibited human c-Fms kinase domain.

Mark Walter, Isabelle S Lucet, Onisha Patel, Sophie E Broughton, Rebecca Bamert, Neal K Williams, Emmanuelle Fantino, Andrew F Wilks, Jamie Rossjohn

Journal article  |  2007  |  Journal of Molecular Biology

c-Fms, a member of the Platelet-derived Growth Factor (PDGF) receptor family of receptor tyrosine kinases (RTKs), is the receptor ..

Hijacking of a substrate-binding protein scaffold for use in mycobacterial cell wall biosynthesis.

Zara Marland, Travis Beddoe, Leyla Zaker-Tabrizi, Isabelle S Lucet, Rajini Brammananth, James C Whisstock, Matthew CJ Wilce, Ross L Coppel, Paul K Crellin, Jamie Rossjohn

Journal article  |  2006  |  Journal of Molecular Biology

The waxy cell wall is crucial to the survival of mycobacteria within the infected host. The cell wall is a complex structure rich ..

Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological function.

Vicki Adams, Isabelle S Lucet, Fleur E Tynan, Martina Chiarezza, Pauline M Howarth, Jonathan Kim, Jamie Rossjohn, Dena Lyras, Julian I Rood

Journal article  |  2006  |  Molecular Microbiology

The large serine recombinase, TnpX, from the Clostridium perfringens integrative mobilizable element Tn4451, consists of three dom..

The structural basis of Janus kinase 2 inhibition by a potent and specific pan-Janus kinase inhibitor.

Isabelle S Lucet, Emmanuelle Fantino, Michelle Styles, Rebecca Bamert, Onisha Patel, Sophie E Broughton, Mark Walter, Christopher J Burns, Herbert Treutlein, Andrew F Wilks, Jamie Rossjohn

Journal article  |  2006  |  Blood

JAK2, a member of the Janus kinase (JAK) family of protein tyrosine kinases (PTKs), is an important intracellular mediator of cyto..

Identification of the structural and functional domains of the large serine recombinase TnpX from Clostridium perfringens.

Isabelle S Lucet, Fleur E Tynan, Vicki Adams, Jamie Rossjohn, Dena Lyras, Julian I Rood

Journal article  |  2005  |  Journal of Biological Chemistry

Members of the large serine resolvase family of site-specific recombinases are responsible for the movement of several mobile gene..

DNA binding properties of TnpX indicate that different synapses are formed in the excision and integration of the Tn4451 family.

Vicki Adams, Isabelle S Lucet, Dena Lyras, Julian I Rood

Journal article  |  2004  |  Molecular Microbiology

Site-specific recombination is an important mechanism for genetic exchange. Insertional recombination mediated by the recently del..

The large resolvase TnpX is the only transposon-encoded protein required for transposition of the Tn4451/3 family of integrative mobilizable elements.

Dena Lyras, Vicki Adams, Isabelle Lucet, Julian I Rood

Journal article  |  2004  |  Molecular Microbiology

Chloramphenicol resistance in Clostridium perfringens and Clostridium difficile is often encoded by catP genes located within the ..

The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringens.

Sheena McGowan, Isabelle S Lucet, Jackie K Cheung, Milena M Awad, James C Whisstock, Julian I Rood

Journal article  |  2002  |  Journal of Molecular Biology

The VirSR two-component signal transduction pathway regulates virulence and toxin production in Clostridium perfringens, the causa..

Cytological and biochemical characterization of the FtsA cell division protein of Bacillus subtilis.

A Feucht, I Lucet, MD Yudkin, J Errington

Journal article  |  2001  |  Molecular Microbiology

The actin-like protein FtsA is present in many eubacteria, and genetic experiments have shown that it plays an important, sometime..

Fate of the SpoIIAB*-ADP liberated after SpoIIAB phosphorylates SpoIIAA of Bacillus subtilis.

CS Lee, I Lucet, MD Yudkin

Journal article  |  2000  |  Journal of Bacteriology

Phosphorylation of SpoIIAA catalyzed by SpoIIAB helps to regulate the first sporulation-specific sigma factor, sigma(F), of Bacill..

Direct interaction between the cell division protein FtsZ and the cell differentiation protein SpoIIE.

I Lucet, A Feucht, MD Yudkin, J Errington

Journal article  |  2000  |  The EMBO Journal

SpoIIE is a bifunctional protein with two critical roles in the establishment of cell fate in Bacillus subtilis. First, SpoIIE is ..

Genotype, phenotype, and protein structure in a regulator of sporulation: effects of mutations in the spoIIAA gene of Bacillus subtilis.

D Barillà, I Lucet, A Kuhlmann, MD Yudkin

Journal article  |  1999  |  Journal of Bacteriology

SpoIIAA, a phosphorylatable protein, is essential to the regulation of sigmaF, the first sporulation-specific transcription factor..

The Bacillus subtilis regulator protein SpoIIE shares functional and structural similarities with eukaryotic protein phosphatases 2C.

R Schroeter, S Schlisio, I Lucet, M Yudkin, R Borriss

Journal article  |  1999  |  FEMS Microbiology Letters

Dephosphorylation of SpoIIAA-P by SpoIIE is strictly dependent on the presence of the bivalent metal ions Mn2+ or Mg2+. Replacemen..

Purification, kinetic properties, and intracellular concentration of SpoIIE, an integral membrane protein that regulates sporulation in Bacillus subtilis.

I Lucet, R Borriss, MD Yudkin

Journal article  |  1999  |  Journal of Bacteriology

SpoIIE is a bifunctional protein which controls sigmaF activation and formation of the asymmetric septum in sporulating Bacillus s..

NAD kinase activities in Euglena gracilis and Phaseolus vulgaris

MC Morere-Le Paven, F Montrichard, I Lucet, R Jalouzot, DL Laval-Martin

Journal article  |  1997  |  Biologia plantarum