Isabelle Lucet

Structural basis for small molecule targeting of Doublecortin Like Kinase 1 with DCLK1-IN-1

Onisha Patel, Michael J Roy, Ashleigh Kropp, Joshua M Hardy, Weiwen Dai, Isabelle S Lucet

Journal article  |  2021  |  COMMUNICATIONS BIOLOGY

Doublecortin-like kinase 1 (DCLK1) is an understudied bi-functional kinase with a proven role in tumour growth and development. Ho..

The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs.

Lung-Yu Liang, Michael Roy, Christopher R Horne, Jarrod J Sandow, Minglyanna Surudoi, Laura F Dagley, Samuel N Young, Toby Dite, Jeffrey J Babon, Peter W Janes, Onisha Patel, James M Murphy, Isabelle S Lucet

Journal article  |  2021  |  Biochem J

EphB6 and EphA10 are two poorly characterised pseudokinase members of the Eph receptor family, which collectively serves as mediat..

Meeting reports: Kinases and pseudokinases online symposium

C Dodson, P Eyers, I Lucet, J Murphy

Journal article  |  2021  |  Biochemist

Ubiquitylation of MLKL at lysine 219 positively regulates necroptosis-induced tissue injury and pathogen clearance

Laura Ramos Garcia, Tencho Tenev, Richard Newman, Rachel O Haich, Gianmaria Liccardi, Sidonie Wicky John, Alessandro Annibaldi, Lu Yu, Mercedes Pardo, Samuel N Young, Cheree Fitzgibbon, Winnie Fernando, Naomi Guppy, Hyojin Kim, Lung-Yu Liang, Isabelle S Lucet, Andrew Kueh, Ioannis Roxanis, Patrycja Gazinska, Martin Sims, et al.

Journal article  |  2021  |  NATURE COMMUNICATIONS

Necroptosis is a lytic, inflammatory form of cell death that not only contributes to pathogen clearance but can also lead to disea..

Granulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory alpha C helix

Michael R Oliver, Christopher R Horne, Safal Shrestha, Jeremy R Keown, Lung-Yu Liang, Samuel N Young, Jarrod J Sandow, Andrew Webb, David C Goldstone, Isabelle S Lucet, Natarajan Kannan, Peter Metcalf, James M Murphy

Journal article  |  2021  |  NATURE COMMUNICATIONS

The life cycle of Baculoviridae family insect viruses depends on the viral protein kinase, PK-1, to phosphorylate the regulatory p..

Development and application of a high-throughput screening assay for identification of small molecule inhibitors of the P. falciparum reticulocyte binding-like homologue 5 protein

Brad E Sleebs, Kate E Jarman, Sonja Frolich, Wilson Wong, Julie Healer, Weiwen Dai, Isabelle S Lucet, Danny W Wilson, Alan F Cowman

Journal article  |  2020  |  INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE

The P. falciparum parasite, responsible for the disease in humans known as malaria, must invade erythrocytes to provide an environ..

A regulatory region on RIPK2 is required for XIAP binding and NOD signaling activity

Valentin J Heim, Laura F Dagley, Che A Stafford, Fynn M Hansen, Elise Clayer, Aleksandra Bankovacki, Andrew Webb, Isabelle S Lucet, John Silke, Ueli Nachbur

Journal article  |  2020  |  EMBO REPORTS

Signaling via the intracellular pathogen receptors nucleotide-binding oligomerization domain-containing proteins NOD1 and NOD2 req..

Potent Inhibition of Necroptosis by Simultaneously Targeting Multiple Effectors of the Pathway

Catia L Pierotti, Maria C Tanzer, Annette Jacobsen, Joanne M Hildebrand, Jean-Marc Garnier, Pooja Sharma, Isabelle S Lucet, Angus D Cowan, Wilhelmus JA Kersten, Meng-Xiao Luo, Lung-Yu Liang, Cheree Fitzgibbon, Sarah E Garnish, Anne Hempel, Ueli Nachbur, David CS Huang, Peter E Czabotar, John Silke, Mark F van Delft, James M Murphy, et al.

Journal article  |  2020  |  ACS CHEMICAL BIOLOGY

Necroptosis is an inflammatory form of programmed cell death that has been implicated in various human diseases. Compound 2 is a m..

Distinct pseudokinase domain conformations underlie divergent activation mechanisms among vertebrate MLKL orthologues

Katherine A Davies, Cheree Fitzgibbon, Samuel N Young, Sarah E Garnish, Wayland Yeung, Diane Coursier, Richard W Birkinshaw, Jarrod J Sandow, Wil IL Lehmann, Lung-Yu Liang, Isabelle S Lucet, James D Chalmers, Wayne M Patrick, Natarajan Kannan, Emma J Petrie, Peter E Czabotar, James M Murphy

Journal article  |  2020  |  NATURE COMMUNICATIONS

The MLKL pseudokinase is the terminal effector in the necroptosis cell death pathway. Phosphorylation by its upstream regulator, R..

An Atlas of Phosphorylation and Proteolytic Processing Events During Excitotoxic Neuronal Death Reveals New Therapeutic Opportunities

Sadia Ameen, Antoine Dufour, Iqbal Hossain, Ashfaqul Hoque, Sharelle Sturgeon, Harshal Nandurkar, Dominik Draxler, Robert Medcalf, Mohd Aizuddin Kamaruddin, Isabelle Lucet, Michael Leeming, Dazhi Liu, Amardeep Dhillon, Jet Phey Lim, Hong-Jian Zhu, Laita Bokhari, Carli Roulston, Oded Kleifeld, Ciccotosto Giuseppe, Nicholas Williamson, et al.

Journal article  |  2020

Summary Excitotoxicity, a neuronal death process in neurological disorders, is initiated by over-stimulation of neuronal ionotropi..