David Stroud is an NHMRC Career Development Fellow and laboratory head within the Department of Biochemistry and Molecular Biology, University of Melbourne. He has an interest in the development and application of new technologies to better understand the assembly of multi-protein membrane complexes, with a focus on mitochondria.
David was awarded his doctorate from the University of Freiburg, Germany in 2011 under the supervision of Prof. Klaus Pfanner. In 2012 David returned to Australia and was awarded an NHMRC Peter Doherty ECR Fellowship. Since then, his work has been focused on mitochondrial respiratory chain assembly and miss-assembly in disease. Under the mentorship of Prof. Mike Ryan, David established a mitochondrial systems biology research program which moved to The University of Melbourne in 2018. David's work combines traditional biochemistry and molecular biology techniques with an in-house proteomics pipeline, and he routinely employs technologies such as SILAC labelling, whole cell proteomes and affinity enrichment mass-spectrometry (including proximity tagging approaches such BioID) to answer basic biological questions.
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David Stroud's selected work
Mass-Spectrometry Based Prioritisation Of Variants Of Unknown Significance In The Diagnosi..
Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomec..
Deciphering The Pathogenetics Of Rare Diseases By Multi-Omic Approaches: Disorders Of Mito..
Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly (MCIA) Complex Facto..
Displaying the 6 most recent projects by David Stroud.
Displaying the 46 most recent scholarly works by David Stroud.
The Mitochondrial Acyl-carrier Protein Interaction Network Highlights Important Roles for LYRM Family Members in Complex I and Mitoribosome Assembly.
Marris G Dibley, Luke E Formosa, Baobei Lyu, Boris Reljic, Dylan McGann, Linden Muellner-Wong, Felix Kraus, Alice J Sharpe, David A Stroud, Michael T Ryan
Journal article | 2020 | Molecular & Cellular Proteomics
NDUFAB1 is the mitochondrial acyl carrier protein (ACP) essential for cell viability. Through its pantetheine-4'-phosphate post-tr..
Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjaerg syndrome
Yilin Kang, Alexander J Anderson, Thomas Daniel Jackson, Catherine S Palmer, David P De Souza, Kenji M Fujihara, Tegan Stait, Ann E Frazier, Nicholas J Clemons, Deidreia Tull, David R Thorburn, Malcolm J McConville, Michael T Ryan, David A Stroud, Diana Stojanovski
Journal article | 2019 | eLife
Human Tim8a and Tim8b are members of an intermembrane space chaperone network, known as the small TIM family. Mutations in TIMM8A ..
Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly (MCIA) Complex Factors in the Biogenesis of Complex I
Luke Formosa, Linden Muellner-Wong, Boris Reljic, Alice Sharpe, Traude Beilharz, Michael Lazarou, David Stroud, Michael Ryan
Journal article | 2019
ABSTRACT Mitochondrial Complex I harbors 7 mitochondrial and 38 nuclear-encoded subunits. Its biogenesis requires the assembly and..
Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6
Shadi Maghool, N Dinesha G Cooray, David A Stroud, David Aragao, Michael T Ryan, Megan J Maher
Journal article | 2019 | Life Science Alliance
Assembly factors play key roles in the biogenesis of many multi-subunit protein complexes regulating their stability, activity, an..
HIGD2A is required for assembly of the COX3 module of human mitochondrial complex IV
Daniella Hock, Boris Reljic, Ching-Seng Ang, Hayley Mountford, Alison Compton, Michael Ryan, David Thorburn, David Stroud
Journal article | 2019
Abstract Assembly factors play a critical role in the biogenesis of the mitochondrial respiratory chain, which includes complexes ..
A patient with homozygous nonsense variants in two Leigh syndrome disease genes: Distinguishing a dual diagnosis from a hypomorphic protein-truncating variant
Nicole J Lake, Luke E Formosa, David A Stroud, Michael T Ryan, Sarah E Calvo, Vamsi K Mootha, Bharti Morar, Peter G Procopis, John Christodoulou, Alison G Compton, David R Thorburn
Journal article | 2019 | Human Mutation
Leigh syndrome is a mitochondrial disease caused by pathogenic variants in over 85 genes. Whole exome sequencing of a patient with..
OXA1L mutations cause mitochondrial encephalopathy and a combined oxidative phosphorylation defect.
Kyle Thompson, Nicole Mai, Monika Oláhová, Filippo Scialó, Luke E Formosa, David A Stroud, Madeleine Garrett, Nichola Z Lax, Fiona M Robertson, Cristina Jou, Andres Nascimento, Carlos Ortez, Cecilia Jimenez-Mallebrera, Steven A Hardy, Langping He, Garry K Brown, Paula Marttinen, Robert McFarland, Alberto Sanz, Brendan J Battersby, et al.
Journal article | 2018 | EMBO Molecular Medicine
OXA1, the mitochondrial member of the YidC/Alb3/Oxa1 membrane protein insertase family, is required for the assembly of oxidative ..
Loss of the Mitochondrial Fatty Acid beta-Oxidation Protein Medium-Chain Acyl-Coenzyme A Dehydrogenase Disrupts Oxidative Phosphorylation Protein Complex Stability and Function
Sze Chern Lim, Makiko Tajika, Masaru Shimura, Kirstyn T Carey, David A Stroud, Kei Murayama, Akira Ohtake, Matthew McKenzie
Journal article | 2018 | Scientific Reports
Loss of BIM increases mitochondrial oxygen consumption and lipid oxidation, reduces adiposity and improves insulin sensitivity in mice.
Jibran A Wali, Sandra Galic, Christina Yr Tan, Esteban N Gurzov, Ann E Frazier, Timothy Connor, Jingjing Ge, Evan G Pappas, David Stroud, L Chitra Varanasi, Claudia Selck, Michael T Ryan, David R Thorburn, Bruce E Kemp, Balasubramanian Krishnamurthy, Thomas Wh Kay, Sean L McGee, Helen E Thomas
Journal article | 2018 | Cell death and differentiation
BCL-2 proteins are known to engage each other to determine the fate of a cell after a death stimulus. However, their evolutionary ..
Loss of BIM increases mitochondrial oxygen consumption and lipid oxidation, reduces adiposity and improves insulin sensitivity in mice
Jibran A Wali, Sandra Galic, Christina YR Tan, Esteban N Gurzov, Ann E Frazier, Timothy Connor, Jingjing Ge, Evan G Pappas, David Stroud, L Chitra Varanasi, Claudia Selck, Michael T Ryan, David R Thorburn, Bruce E Kemp, Balasubramanian Krishnamurthy, Thomas WH Kay, Sean L Mcgee, Helen E Thomas
Journal article | 2018 | Cell Death & Differentiation
Myoepithelial cell-specific expression of stefin A as a suppressor of early breast cancer invasion
Hendrika M Duivenvoorden, Jai Rautela, Laura E Edgington-Mitchell, Alex Spurling, David W Greening, Cameron J Nowell, Timothy J Molloy, Elizabeth Robbins, Natasha K Brockwell, Cheok Soon Lee-, Maoshan Chen, Anne Holliday, Cristina I Selinger, Min Hu, Kara L Britt, David A Stroud, Matthew Bogyo, Andreas Moeller, Kornelia Polyak, Bonnie F Sloane, et al.
Journal article | 2017 | The Journal of Pathology
Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome
Nicole J Lake, Bryn D Webb, David A Stroud, Tara R Richman, Benedetta Ruzzenente, Alison G Compton, Hayley S Mountford, Juliette Pulman, Coralie Zangarelli, Marlene Rio, Nathalie Bodaert, Zahra Assouline, Mingma D Sherpa, Eric E Schadt, Sander M Houten, James Byrnes, Elizabeth M McCormick, Zarazuela Zolkipli-Cunningham, Katrina Haude, Zhancheng Zhang, et al.
Journal article | 2017 | The American Journal of Human Genetics
Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex
Yilin Kang, David A Stroud, Michael J Baker, David P De Souza, Ann E Frazier, Michael Liem, Dedreia Tull, Suresh Mathivanan, Malcolm J McConville, David R Thorburn, Michael T Ryan, Diana Stojanovski
Journal article | 2017 | Molecular Cell
Principles of Systems Biology, No. 11
Greg Wayne, Alex Graves, Demis Hassabis, Shambaditya Saha, Christoph A Weber, Anthony A Hyman, Frank Juelicher, David A Stroud, Michael T Ryan, Laurent Potvin-Trottier, Johan Paulsson, Kerry A Geiler-Samerotte, Mark L Siegal, Mathias Uhlen, Fredrik Edfors, Bjorn Forsstrom, Kole Roybal, Wendell Lim, Vincent Libis, Baudoin Delepine, et al.
Journal article | 2016 | Cell Systems
Accessory subunits are integral for assembly and function of human mitochondrial complex I
David A Stroud, Elliot E Surgenor, Luke E Formosa, Boris Reljic, Ann E Frazier, Marris G Dibley, Laura D Osellame, Tegan Stait, Traude H Beilharz, David R Thorburn, Agus Salim, Michael T Ryan
Journal article | 2016 | Nature
COA6 is a mitochondrial complex IV assembly factor critical for biogenesis of mtDNA-encoded COX2
David A Stroud, Megan J Maher, Caroline Lindau, F-Nora Voegtle, Ann E Frazier, Elliot Surgenor, Hayley Mountford, Abeer P Singh, Matteo Bonas, Silke Oeljeklaus, Bettina Warscheid, Chris Meisinger, David R Thorburn, Michael T Ryan
Journal article | 2015 | Human Molecular Genetics
FunRich: An open access standalone functional enrichment and interaction network analysis tool
Mohashin Pathan, Shivakumar Keerthikumar, Ching-Seng Ang, Lahiru Gangoda, Camelia YJ Quek, Nicholas A Williamson, Dmitri Mouradov, Oliver M Sieber, Richard J Simpson, Agus Salim, Antony Bacic, Andrew F Hill, David A Stroud, Michael T Ryan, Johnson I Agbinya, John M Mariadason, Antony W Burgess, Suresh Mathivanan
Journal article | 2015 | PROTEOMICS
Bax targets mitochondria by distinct mechanisms before or during apoptotic cell death: a requirement for VDAC2 or Bak for efficient Bax apoptotic function
SB Ma, TN Nguyen, I Tan, R Ninnis, S Iyer, DA Stroud, M Menard, RM Kluck, MT Ryan, G Dewson
Journal article | 2014 | Cell Death & Differentiation
A Founder Mutation in PET100 Causes Isolated Complex IV Deficiency in Lebanese Individuals with Leigh Syndrome
Sze Chern Lim, Katherine R Smith, David A Stroud, Alison G Compton, Elena J Tucker, Ayan Dasvarma, Luke C Gandolfo, Justine E Marum, Matthew McKenzie, Heidi L Peters, David Mowat, Peter G Procopis, Bridget Wilcken, John Christodoulou, Garry K Brown, Michael T Ryan, Melanie Bahlo, David R Thorburn
Journal article | 2014 | American Journal of Human Genetics
Coupling of Mitochondrial Import and Export Translocases by Receptor-Mediated Supercomplex Formation
Jian Qiu, Lena-Sophie Wenz, Ralf M Zerbes, Silke Oeljeklaus, Maria Bohnert, David A Stroud, Christophe Wirth, Lars Ellenrieder, Nicolas Thornton, Stephan Kutik, Sebastian Wiese, Agnes Schulze-Specking, Nicole Zufall, Agnieszka Chacinska, Bernard Guiard, Carola Hunte, Bettina Warscheid, Martin van der Laan, Nikolaus Pfanner, Nils Wiedemann, et al.
Journal article | 2013 | Cell
Gene Knockout Using Transcription Activator-like Effector Nucleases (TALENs) Reveals That Human NDUFA9 Protein Is Essential for Stabilizing the Junction between Membrane and Matrix Arms of Complex I
David A Stroud, Luke E Formosa, Xiaonan W Wijeyeratne, Thanh N Nguyen, Michael T Ryan
Journal article | 2013 | Journal of Biological Chemistry
Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane
Maria Bohnert, Lena-Sophie Wenz, Ralf M Zerbes, Susanne E Horvath, David A Stroud, Karina von der Malsburg, Judith M Mueller, Silke Oeljeklaus, Inge Perschil, Bettina Warscheid, Agnieszka Chacinska, Marten Veenhuis, Ida J van der Klei, Guenther Daum, Nils Wiedemann, Thomas Becker, Nikolaus Pfanner, Martin van der Laan
Journal article | 2012 | Molecular Biology of the Cell
Role of MINOS in Mitochondrial Membrane Architecture: Cristae Morphology and Outer Membrane Interactions Differentially Depend on Mitofilin Domains
Ralf M Zerbes, Maria Bohnert, David A Stroud, Karina von der Malsburg, Anita Kram, Silke Oeljeklaus, Bettina Warscheid, Thomas Becker, Nils Wiedemann, Marten Veenhuis, Ida J van der Klei, Nikolaus Pfanner, Martin van der Laan
Journal article | 2012 | Journal of Molecular Biology
Dual Function of Sdh3 in the Respiratory Chain and TIM22 Protein Translocase of the Mitochondrial Inner Membrane
Natalia Gebert, Michael Gebert, Silke Oeljeklaus, Karina von der Malsburg, David A Stroud, Bogusz Kulawiak, Christophe Wirth, Rene P Zahedi, Pavel Dolezal, Sebastian Wiese, Oliver Simon, Agnes Schulze-Specking, Kaye N Truscott, Albert Sickmann, Peter Rehling, Bernard Guiard, Carola Hunte, Bettina Warscheid, Martin van der Laan, Nikolaus Pfanner, et al.
Journal article | 2011 | Molecular Cell
Composition and Topology of the Endoplasmic Reticulum-Mitochondria Encounter Structure
David A Stroud, Silke Oeljeklaus, Sebastian Wiese, Maria Bohnert, Urs Lewandrowski, Albert Sickmann, Bernard Guiard, Martin van der Laan, Bettina Warscheid, Nils Wiedemann
Journal article | 2011 | Journal of Molecular Biology
Biogenesis of mitochondrial beta-barrel proteins: the POTRA domain is involved in precursor release from the SAM complex
David A Stroud, Thomas Becker, Jian Qiu, Diana Stojanovski, Sylvia Pfannschmidt, Christophe Wirth, Carola Hunte, Bernard Guiard, Chris Meisinger, Nikolaus Pfanner, Nils Wiedemann
Journal article | 2011 | Molecular Biology of the Cell
Mitochondrial Cardiolipin Involved in Outer-Membrane Protein Biogenesis: Implications for Barth Syndrome
Natalia Gebert, Amit S Joshi, Stephan Kutik, Thomas Becker, Matthew McKenzie, Xue Li Guan, Ved P Mooga, David A Stroud, Gnanada Kulkarni, Markus R Wenk, Peter Rehling, Chris Meisinger, Michael T Ryan, Nils Wiedemann, Miriam L Greenberg, Nikolaus Pfanner
Journal article | 2009 | Current Biology
Structural and Functional Requirements for Activity of the Tim9-Tim10 Complex in Mitochondrial Protein Import
Michael J Baker, Chaille T Webb, David A Stroud, Catherine S Palmer, Ann E Frazier, Bernard Guiard, Agnieszka Chacinska, Jacqueline M Gulbis, Michael T Ryan
Journal article | 2009 | Molecular Biology of the Cell
The TIM22 complex regulates mitochondrial one-carbon metabolism by mediating the import of Sideroflexins
Thomas D Jackson, Daniella Hock, Catherine S Palmer, Yilin Kang, Kenji M Fujihara, Nicholas J Clemons, David R Thorburn, David A Stroud, Diana Stojanovski
AbstractThe Acylglycerol Kinase (AGK) is a mitochondrial lipid kinase that contributes to protein biogenesis as a subunit of the T..
Honours, Awards and Fellowships
NHMRCF - NHMRC Fellowship
NHMRCF - NHMRC Fellowship
Senior Research Fellow
Biochemistry And Molecular Biology
Australian Society of Biochemistry & Molecular Biology
Australian Proteomics Society
Doctor rerum naturalium
University of Freiburg
Bachelor of Science (Honours in Biochemistry)
La Trobe University