Sara Howden

DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets

Sean B Wilson, Sara E Howden, Jessica M Vanslambrouck, Aude Dorison, Jose Alquicira-Hernandez, Joseph E Powell, Melissa H Little

Journal article  |  2022  |  GENOME MEDICINE

BACKGROUND: While single-cell transcriptional profiling has greatly increased our capacity to interrogate biology, accurate cell c..

Determining lineage relationships in kidney development and disease

Melissa H Little, Sara E Howden, Kynan T Lawlor, Jessica M Vanslambrouck

Journal article  |  2021  |  NATURE REVIEWS NEPHROLOGY

The lineage relationships of cells provide information about the origins of component cell types during development and repair as ..

Generating an iPSC line (with isogenic control) from the PBMCs of an ACTA1 (p.Gly148Asp) nemaline myopathy patient

Peter J Houweling, Chantal A Coles, Chrystal F Tiong, Bridget Nielsen, Alison Graham, Penny McDonald, Annabelle Suter, Adam T Piers, Robin Forbes, Monique M Ryan, Sara E Howden, Shireen R Lamande, Kathryn N North

Journal article  |  2021  |  STEM CELL RESEARCH

To produce an in vitro model of nemaline myopathy, we reprogrammed the peripheral blood mononuclear cells (PBMCs) of a patient wit..

Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk

Sara E Howden, Sean B Wilson, Ella Groenewegen, Lakshi Starks, Thomas A Forbes, Ker Sin Tan, Jessica M Vanslambrouck, Emily M Holloway, Yi-Hsien Chen, Sanjay Jain, Jason R Spence, Melissa H Little

Journal article  |  2021  |  CELL STEM CELL

During development, distinct progenitors contribute to the nephrons versus the ureteric epithelium of the kidney. Indeed, previous..

In vivo survival and differentiation of Friedreich ataxia iPSC-derived sensory neurons transplanted in the adult dorsal root ganglia

Serena Viventi, Stefano Frausin, Sara E Howden, Shiang Y Lim, Rocio K Finol-Urdaneta, Jeffrey R McArthur, Kwaku Dad Abu-Bonsrah, Wayne Ng, Jason Ivanusic, Lachlan Thompson, Mirella Dottori

Journal article  |  2021  |  STEM CELLS TRANSLATIONAL MEDICINE

Friedreich ataxia (FRDA) is an autosomal recessive disease characterized by degeneration of dorsal root ganglia (DRG) sensory neur..

Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice

Amar J Majmundar, Florian Buerger, Thomas A Forbes, Verena Klambt, Ronen Schneider, Konstantin Deutsch, Thomas M Kitzler, Sara E Howden, Michelle Scurr, Ker Sin Tan, Mickael Krzeminski, Eugen Widmeier, Daniela A Braun, Ethan Lai, Ihsan Ullah, Ali Amar, Amy Kolb, Kaitlyn Eddy, Chin Heng Chen, Daanya Salmanullah, et al.

Journal article  |  2021  |  SCIENCE ADVANCES

Nephrotic syndrome (NS) is a leading cause of chronic kidney disease. We found recessive NOS1AP variants in two families with earl..

Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation

Kynan T Lawlor, Jessica M Vanslambrouck, J William Higgins, Alison Chambon, Kristina Bishard, Derek Arndt, Pei Xuan Er, Sean B Wilson, Sara E Howden, Ker Sin Tan, Fanyi Li, Lorna J Hale, Benjamin Shepherd, Stephen Pentoney, Sharon C Presnell, Alice E Chen, Melissa H Little

Journal article  |  2020  |  NATURE MATERIALS

Directed differentiation of human pluripotent stem cells to kidney organoids brings the prospect of drug screening, disease modell..

Particle-mediated delivery of frataxin plasmid to a human sensory neuronal model of Friedreich's ataxia.

Ewa Czuba-Wojnilowicz, Serena Viventi, Sara E Howden, Simon Maksour, Amy E Hulme, Christina Cortez-Jugo, Mirella Dottori, Frank Caruso

Journal article  |  2020  |  Biomaterials Science

Increasing frataxin protein levels through gene therapy is envisaged to improve therapeutic outcomes for patients with Friedreich'..

Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions

Sara E Howden, Melissa H Little

Book Chapter  |  2020  |  Methods in Molecular Biology

The ultimate goal of regenerative medicine is to have access to an unlimited supply of specific cell types on demand, which can be..

A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids

Jessica M Vanslambrouck, Sean B Wilson, Ker Sin Tan, Joanne Y-C Soo, Michelle Scurr, H Siebe Spijker, Lakshi T Starks, Amber Neilson, Xiaoxia Cui, Sanjay Jain, Melissa Helen Little, Sara E Howden

Journal article  |  2019  |  JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

BACKGROUND: The generation of reporter lines for cell identity, lineage, and physiologic state has provided a powerful tool in adv..