Christian Makhoul

Arf5-mediated regulation of mTORC1 at the plasma membrane.

Christian Makhoul, Fiona J Houghton, Elizabeth Hinde, Paul A Gleeson

Journal article  |  2023  |  Mol Biol Cell

The mechanistic target of rapamycin (mTOR) kinase regulates a major signaling pathway in eukaryotic cells. In addition to regulati..

A biosensor of protein foldedness identifies increased "holdase" activity of chaperones in the nucleus following increased cytosolic protein aggregation.

Candice B Raeburn, Angelique R Ormsby, Dezerae Cox, Chloe A Gerak, Christian Makhoul, Nagaraj S Moily, Simon Ebbinghaus, Alex Dickson, Gawain McColl, Danny M Hatters

Journal article  |  2022  |  J Biol Chem

Chaperones and other quality control machinery guard proteins from inappropriate aggregation, which is a hallmark of neurodegenera..

Interacting partners of Golgi-localized small G protein Arl5b identified by a combination of in vivo proximity labelling and GFP-Trap pull down

Fiona J Houghton, Christian Makhoul, Ellie Hyun-Jung Cho, Nicholas A Williamson, Paul A Gleeson

Journal article  |  2022  |  FEBS LETTERS

The small G protein Arl5b is localised on the trans-Golgi network (TGN) and regulates endosomes-to-TGN transport. Here, we combine..

Regulation of mTORC1 activity by the Golgi apparatus.

Christian Makhoul, Paul A Gleeson

Journal article  |  2021  |  Fac Rev

Mechanistic (or mammalian) target of rapamycin complex 1 (mTORC1) is a major signalling kinase in cells that regulates proliferati..

Form and function of the Golgi apparatus: scaffolds, cytoskeleton and signalling

Prajakta Kulkarni-Gosavi, Christian Makhoul, Paul A Gleeson

Journal article  |  2019  |  FEBS LETTERS

In addition to the classical functions of the Golgi in membrane transport and glycosylation, the Golgi apparatus of mammalian cell..

Golgi Dynamics: The Morphology of the Mammalian Golgi Apparatus in Health and Disease

Christian Makhoul, Prajakta Gosavi, Paul A Gleeson

Journal article  |  2019  |  FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY

In vertebrate cells the Golgi consists of individual stacks fused together into a compact ribbon structure. The function of the ri..

Intersectin-1 interacts with the golgin GCC88 to couple the actin network and Golgi architecture

Christian Makhoul, Prajakta Gosavi, Regina Duffield, Bronwen Delbridge, Nicholas A Williamson, Paul A Gleeson

Journal article  |  2019  |  MOLECULAR BIOLOGY OF THE CELL

The maintenance of the Golgi ribbon relies on a dynamic balance between the actin and microtubule networks; however, the pathways ..

The Golgi architecture and cell sensing

Christian Makhoul, Prajakta Gosavi, Paul A Gleeson

Journal article  |  2018  |  BIOCHEMICAL SOCIETY TRANSACTIONS

An array of signalling molecules are located at the Golgi apparatus, including phosphoinositides, small GTPases, kinases, and phos..