Jason Brouwer

Structure of detergent-activated BAK dimers derived from the inert monomer

Richard W Birkinshaw, Sweta Iyer, Daisy Lio, Cindy S Luo, Jason M Brouwer, Michelle S Miller, Adeline Y Robin, Rachel T Uren, Grant Dewson, Ruth M Kluck, Peter M Colman, Peter E Czabotar

Journal article  |  2021  |  MOLECULAR CELL

A body of data supports the existence of core (α2-α5) dimers of BAK and BAX in the oligomeric, membrane-perturbing conformation of..

BAK core dimers bind lipids and can be bridged by them

Angus D Cowan, Nicholas A Smith, Jarrod J Sandow, Eugene A Kapp, Yepy H Rustam, James M Murphy, Jason M Brouwer, Jonathan P Bernardini, Michael J Roy, Ahmad Z Wardak, Iris K Tan, Andrew I Webb, Jacqueline M Gulbis, Brian J Smith, Gavin E Reid, Grant Dewson, Peter M Colman, Peter E Czabotar

Journal article  |  2020  |  NATURE STRUCTURAL & MOLECULAR BIOLOGY

BAK and BAX are essential mediators of apoptosis that oligomerize in response to death cues, thereby causing permeabilization of t..

A small molecule interacts with VDAC2 to block mouse BAK-driven apoptosis

Mark F van Delft, Stephane Chappaz, Yelena Khakham, T Bui Chinh, Marlyse A Debrincat, Kym N Lowes, Jason M Brouwer, Christoph Grohmann, Phillip P Sharp, Laura F Dagley, Lucy Li, Kate McArthur, Meng-Xiao Luo, Hui San Chin, W Douglas Fairlie, Erinna F Lee, David Segal, Stephane Duflocq, Romina Lessene, Sabrina Bernard, et al.

Journal article  |  2019  |  NATURE CHEMICAL BIOLOGY

Activating the intrinsic apoptosis pathway with small molecules is now a clinically validated approach to cancer therapy. In contr..

Parkin inhibits BAK and BAX apoptotic function by distinct mechanisms during mitophagy

Jonathan P Bernardini, Jason M Brouwer, Iris KL Tan, Jarrod J Sandow, Shuai Huang, Che A Stafford, Aleksandra Bankovacki, Christopher D Riffkin, Ahmad Z Wardak, Peter E Czabotar, Michael Lazarou, Grant Dewson

Journal article  |  2019  |  EMBO JOURNAL

The E3 ubiquitin ligase Parkin is a key effector of the removal of damaged mitochondria by mitophagy. Parkin determines cell fate ..

Conversion of Bim-BH3 from Activator to Inhibitor of Bak through Structure-Based Design

Jason M Brouwer, Ping Lan, Angus D Cowan, Jonathan P Bernardini, Richard W Birkinshaw, Mark F van Delft, Brad E Sleebs, Adeline Y Robin, Ahmad Wardak, Iris K Tan, Boris Reljic, Erinna F Lee, W Douglas Fairlie, Melissa J Call, Brian J Smith, Grant Dewson, Guillaume Lessene, Peter M Colman, Peter E Czabotar

Journal article  |  2017  |  MOLECULAR CELL

Certain BH3-only proteins transiently bind and activate Bak and Bax, initiating their oligomerization and the permeabilization of ..

Disordered clusters of Bak dimers rupture mitochondria during apoptosis

Rachel T Uren, Martin O'Hely, Sweta Iyer, Ray Bartolo, Melissa X Shi, Jason M Brouwer, Amber E Alsop, Grant Dewson, Ruth M Kluck

Journal article  |  2017  |  ELIFE

During apoptosis, Bak and Bax undergo major conformational change and form symmetric dimers that coalesce to perforate the mitocho..

Bak Core and Latch Domains Separate during Activation, and Freed Core Domains Form Symmetric Homodimers

Jason M Brouwer, Dana Westphal, Grant Dewson, Adeline Y Robin, Rachel T Uren, Ray Bartolo, Geoff V Thompson, Peter M Colman, Ruth M Kluck, Peter E Czabotar

Journal article  |  2014  |  MOLECULAR CELL

Apoptotic stimuli activate and oligomerize the proapoptotic proteins Bak and Bax, resulting in mitochondrial outer-membrane permea..

Conformational rearrangements of RIG-I receptor on formation of a multiprotein:dsRNA assembly.

Simone A Beckham, Jason Brouwer, Anna Roth, Die Wang, Anthony J Sadler, Matthias John, Kerstin Jahn-Hofmann, Bryan RG Williams, Jacqueline A Wilce, Matthew CJ Wilce

Journal article  |  2013  |  Nucleic Acids Res

The retinoic acid inducible gene-I (RIG-I)-like family of receptors is positioned at the front line of our innate cellular defence..

Engineering a structure switching mechanism into a steroid-binding aptamer and hydrodynamic analysis of the ligand binding mechanism.

Oren Reinstein, Miguel AD Neves, Makbul Saad, Sherry N Boodram, Stephanie Lombardo, Simone A Beckham, Jason Brouwer, Gerald F Audette, Patrick Groves, Matthew CJ Wilce, Philip E Johnson

Journal article  |  2011  |  Biochemistry

The steroid binding mechanism of a DNA aptamer was studied using isothermal titration calorimetry (ITC), NMR spectroscopy, quasi-e..