Liam Sharkey

Staphylococcus aureus host interactions and adaptation

Benjamin P Howden, Stefano G Giulieri, Tania Wong Fok Lung, Sarah L Baines, Liam K Sharkey, Jean YH Lee, Abderrahman Hachani, Ian R Monk, Timothy P Stinear

Journal article  |  2023  |  NATURE REVIEWS MICROBIOLOGY

Invasive Staphylococcus aureus infections are common, causing high mortality, compounded by the propensity of the bacterium to dev..

Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions

Andrew H Buultjens, Koen Vandelannoote, Liam K Sharkey, Benjamin P Howden, Ian R Monk, Jean YH Lee, Timothy P Stinear

Journal article  |  2021  |  ACS BIOMATERIALS SCIENCE & ENGINEERING

The ability to detect SARS-CoV-2 is critical to implementing evidence-based strategies to address the COVID-19 global pandemic. Ex..

Accessible Platform for High-Throughput COVID-19 Molecular Diagnostics and Genome Sequencing Using a Repurposed 3D Printer for RNA Extraction

Koen Vandelannoote, Andrew H Buultjens, Lucy Li, Liam K Sharkey, Marion Herisse, Sacha J Pidot, Hoang Tuyet, Benjamin P Howden, Ian R Monk, Torsten Seemann, Jean YH Lee, Timothy P Stinear

Journal article  |  2021  |  ACS BIOMATERIALS SCIENCE & ENGINEERING

The COVID-19 pandemic has exposed the dependence of diagnostic laboratories on a handful of large corporations with market monopol..

Molecular Mechanisms of Antibiotic Resistance - Part II

Liam KR Sharkey, Alex J O'Neill

Book Chapter  |  2020

All antibiotic resistance mechanisms in bacteria ultimately work by preventing, or mitigating the effects of, an antibacterial inh..

Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus

Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King, et al.

Journal article  |  2019  |  NATURE COMMUNICATIONS

WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidogl..

Biosynthesis and Ether‐Bridge Formation in Nargenicin Macrolides

Sacha J Pidot, Marion Herisse, Liam Sharkey, Liselle Atkin, Jessica L Porter, Torsten Seemann, Benjamin P Howden, Mark A Rizzacasa, Timothy P Stinear

Journal article  |  2019  |  Angewandte Chemie

Abstract: The nargenicin family of antibiotics are macrolides containing a rare ether‐bridged cis‐decalin motif. Several of these ..

Biosynthesis and Ether-Bridge Formation in Nargenicin Macrolides

Sacha J Pidot, Marion Herisse, Liam Sharkey, Liselle Atkin, Jessica L Porter, Torsten Seemann, Benjamin P Howden, Mark A Rizzacasa, Timothy P Stinear

Journal article  |  2019  |  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The nargenicin family of antibiotics are macrolides containing a rare ether-bridged cis-decalin motif. Several of these compounds ..

Antibiotic Resistance ABC-F Proteins: Bringing Target Protection into the Limelight

Liam KR Sharkey, Alex J O'Neill

Journal article  |  2018  |  ACS INFECTIOUS DISEASES

Members of the ATP-binding cassette (ABC)-F protein subfamily collectively mediate resistance to a broader range of clinically imp..

1-((2,4-Dichlorophenethyl)Amino)3-Phenoxypropan-2-ol Kills Pseudomonas aeruginosa through Extensive Membrane Damage

Valerie Defraine, Veerle Liebens, Evelien Loos, Toon Swings, Bram Weytjens, Carolina Fierro, Kathleen Marchal, Liam Sharkey, Alex J O'Neill, Romu Corbau, Arnaud Marchand, Patrick Chaltin, Maarten Fauvart, Jan Michiels

Journal article  |  2018  |  FRONTIERS IN MICROBIOLOGY

The ever increasing multidrug-resistance of clinically important pathogens and the lack of novel antibiotics have resulted in a tr..

ABC-F Proteins Mediate Antibiotic Resistance through Ribosomal Protection

Liam KR Sharkey, Thomas A Edwards, Alex J O'Neill

Journal article  |  2016  |  MBIO

Members of the ABC-F subfamily of ATP-binding cassette proteins mediate resistance to a broad array of clinically important antibi..