Lucas Huggins

A multipronged next-generation sequencing metabarcoding approach unearths hyper-diverse and abundant dog pathogen communities in Cambodia

Lucas G Huggins, Vito Colella, Anson V Koehler, Bettina Schunack, Rebecca J Traub

Journal article  |  2021  |  Transboundary and Emerging Diseases

Recent surveys in Southeast Asia, including Cambodia, have identified canine vector-borne pathogens (VBPs), including those with z..

Novel High-Throughput Multiplex qPCRs for the Detection of Canine Vector-Borne Pathogens in the Asia-Pacific

Lucas Huggins, Luca Massetti, Bettina Schunack, Vito Colella, Rebecca Traub

Journal article  |  2021  |  MICROORGANISMS

The Asia-Pacific hosts a large diversity of canine vector-borne pathogens (VBPs) with some of the most common and most pathogenic,..

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

Lucas G Huggins, Anson Koehler, Bettina Schunack, Tawin Inpankaew, Rebecca J Traub

Journal article  |  2020  |  PATHOGENS

Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and..

A novel metabarcoding diagnostic tool to explore protozoan haemoparasite diversity in mammals: a proof-of-concept study using canines from the tropics

Lucas G Huggins, Anson Koehler, Ng-Nguyen Dinh, Stephen Wilcox, Bettina Schunack, Tawin Inpankaew, Rebecca J Traub

Journal article  |  2019  |  SCIENTIFIC REPORTS

Haemoparasites are responsible for some of the most prevalent and debilitating canine illnesses across the globe, whilst also posi..

Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand

Lucas G Huggins, Anson Koehler, Ng-Nguyen Dinh, Stephen Wilcox, Bettina Schunack, Tawin Inpankaew, Rebecca J Traub

Journal article  |  2019  |  PARASITES & VECTORS

BACKGROUND: Globally, bacterial vector-borne disease (VBD) exerts a large toll on dogs in terms of morbidity and mortality but now..

A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles (vol 12, e0185151, 2017)

Lucas G Huggins, Christopher J Michaels, Sheena M Cruickshank, Richard F Preziosi, Kathryn J Else

Journal article  |  2018  |  PLOS ONE

[This corrects the article DOI: 10.1371/journal.pone.0185151.].

A novel copro-diagnostic molecular method for qualitative detection and identification of parasitic nematodes in amphibians and reptiles

Lucas G Huggins, Christopher J Michaels, Sheena M Cruickshank, Richard F Preziosi, Kathryn J Else

Journal article  |  2017  |  PLOS ONE

Anthropogenic disturbance via resource acquisition, habitat fragmentation and climate change, amongst other factors, has led to ca..