Paula Miotto

Short-term bed rest-induced insulin resistance cannot be explained by increased mitochondrial H2 O2 emission.

Marlou L Dirks, Paula M Miotto, Gijs H Goossens, Joan M Senden, Heather L Petrick, Janneau van Kranenburg, Luc JC van Loon, Graham P Holloway

Journal article  |  2020  |  The Journal of Physiology

KEY POINTS: We determined if bed rest increased mitochondrially derived reactive oxygen species and cellular redox stress, contrib..

The Liver as an Endocrine Organ-Linking NAFLD and Insulin Resistance

Matthew J Watt, Paula M Miotto, William De Nardo, Magdalene K Montgomery

Journal article  |  2019  |  Endocrine Reviews

The liver is a dynamic organ that plays critical roles in many physiological processes, including the regulation of systemic gluco..

Supplementation with dietary ω-3 mitigates immobilization-induced reductions in skeletal muscle mitochondrial respiration in young women.

Paula M Miotto, Chris McGlory, Ravninder Bahniwal, Michael Kamal, Stuart M Phillips, Graham P Holloway

Journal article  |  2019  |  The FASEB Journal

Omega-3 (ω-3) supplementation attenuates immobilization-induced atrophy; however, the underlying mechanisms remain unclear. Since ..

Exercise-induced reductions in mitochondrial ADP sensitivity contribute to the induction of gene expression and mitochondrial biogenesis through enhanced mitochondrial H2O2 emission.

Paula M Miotto, Graham P Holloway

Journal article  |  2019  |  Mitochondrion

Acute exercise rapidly induces mitochondrial gene expression, however, the intracellular events regulating this process remain inc..

High-Fat Diet Causes Mitochondrial Dysfunction as a Result of Impaired ADP Sensitivity.

Paula M Miotto, Paul J LeBlanc, Graham P Holloway

Journal article  |  2018  |  Diabetes

Although molecular approaches altering mitochondrial content have implied a direct relationship between mitochondrial bioenergetic..

The importance of exercise intensity, volume and metabolic signalling events in the induction of mitochondrial biogenesis.

Heather L Petrick, Kaitlyn MJH Dennis, Paula M Miotto

Journal article  |  2018  |  The Journal of Physiology

Mitochondrial-derived reactive oxygen species influence ADP sensitivity, but not CPT-I substrate sensitivity.

Pierre-Andre Barbeau, Paula M Miotto, Graham P Holloway

Journal article  |  2018  |  Biochemical Journal

The mechanisms regulating oxidative phosphorylation during exercise remain poorly defined; however, key mitochondrial proteins, in..

Prior exercise training improves cold tolerance independent of indices associated with non-shivering thermogenesis.

Carly M Knuth, Willem T Peppler, Logan K Townsend, Paula M Miotto, Anders Gudiksen, David C Wright

Journal article  |  2018  |  The Journal of Physiology

KEY POINTS: Mammals defend against cold-induced reductions in body temperature through both shivering and non-shivering thermogene..

Sodium nitrate supplementation alters mitochondrial H2O2 emission but does not improve mitochondrial oxidative metabolism in the heart of healthy rats.

Cynthia MF Monaco, Paula M Miotto, Jason S Huber, Luc JC van Loon, Jeremy A Simpson, Graham P Holloway

Journal article  |  2018  |  American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Supplementation with dietary inorganic nitrate ([Formula: see text]) is increasingly recognized to confer cardioprotective effects..

Sex differences in mitochondrial respiratory function in human skeletal muscle.

Paula M Miotto, Chris McGlory, Tanya M Holloway, Stuart M Phillips, Graham P Holloway

Journal article  |  2018  |  American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Mitochondrial bioenergetic contributions to sex differences in human skeletal muscle metabolism remain poorly defined. The primary..

Age-Associated Impairments in Mitochondrial ADP Sensitivity Contribute to Redox Stress in Senescent Human Skeletal Muscle.

Graham P Holloway, Andrew M Holwerda, Paula M Miotto, Marlou L Dirks, Lex B Verdijk, Luc JC van Loon

Journal article  |  2018  |  Cell Reports

It remains unknown if mitochondrial bioenergetics are altered with aging in humans. We established an in vitro method to simultane..

α-linolenic acid supplementation prevents exercise-induced improvements in white adipose tissue mitochondrial bioenergetics and whole-body glucose homeostasis in obese Zucker rats.

Cynthia MF Monaco, Ross Proudfoot, Paula M Miotto, Eric AF Herbst, Rebecca EK MacPherson, Graham P Holloway

Journal article  |  2018  |  Diabetologia

AIMS/HYPOTHESIS: While the underlying mechanisms in the development of insulin resistance remain inconclusive, metabolic dysfuncti..

Exercise Protects Against Olanzapine-Induced Hyperglycemia in Male C57BL/6J Mice.

Laura N Castellani, Willem T Peppler, Paula M Miotto, Natasha Bush, David C Wright

Journal article  |  2018  |  Scientific Reports

Olanzapine is a widely prescribed antipsychotic drug. While effective in reducing psychoses, treatment with olanzapine causes rapi..

Ablating the protein TBC1D1 impairs contraction-induced sarcolemmal glucose transporter 4 redistribution but not insulin-mediated responses in rats.

Jamie Whitfield, Sabina Paglialunga, Brennan K Smith, Paula M Miotto, Genevieve Simnett, Holly L Robson, Swati S Jain, Eric AF Herbst, Eric M Desjardins, David J Dyck, Lawrence L Spriet, Gregory R Steinberg, Graham P Holloway

Journal article  |  2017  |  Journal of Biological Chemistry

TBC1 domain family member 1 (TBC1D1), a Rab GTPase-activating protein and paralogue of Akt substrate of 160 kDa (AS160), has been ..

Compensatory increases in protein markers of mitochondrial dynamics during ageing are adaptable to physical activity.

Paula M Miotto, Pierre-Andre Barbeau, Stuart Brownell

Journal article  |  2017  |  The Journal of Physiology

α-Linolenic acid supplementation and exercise training reveal independent and additive responses on hepatic lipid accumulation in obese rats.

Paula M Miotto, Meaghan Horbatuk, Ross Proudfoot, Sarthak Matravadia, Marica Bakovic, Adrian Chabowski, Graham P Holloway

Journal article  |  2017  |  American Journal of Physiology-Endocrinology and Metabolism

α-Linolenic acid (ALA) supplementation or exercise training can independently prevent hepatic lipid accumulation and reduced insul..

CL 316, 243 mediated reductions in blood glucose are enhanced in RIP140-/- mice independent of alterations in lipolysis.

Willem T Peppler, Paula M Miotto, Graham P Holloway, David C Wright

Journal article  |  2017  |  Biochemical and Biophysical Research Communications

The β-3 adrenergic agonist CL 316, 243 acutely lowers blood glucose through a mechanism thought to involve fatty-acid induced insu..

Saturation of SERCA's lipid annulus may protect against its thermal inactivation.

Val Andrew Fajardo, Natalie Trojanowski, Laura M Castelli, Paula M Miotto, Foyinsola Amoye, Wendy E Ward, A Russell Tupling, Paul J LeBlanc

Journal article  |  2017  |  Biochemical and Biophysical Research Communications

The sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) pumps are integral membrane proteins that catalyze the active transport of Ca..

Controlling skeletal muscle CPT-I malonyl-CoA sensitivity: the importance of AMPK-independent regulation of intermediate filaments during exercise.

Paula M Miotto, Gregory R Steinberg, Graham P Holloway

Journal article  |  2017  |  Biochemical Journal

The obligatory role of carnitine palmitoyltransferase-I (CPT-I) in mediating mitochondrial lipid transport is well established, a ..

In the absence of phosphate shuttling, exercise reveals the in vivo importance of creatine-independent mitochondrial ADP transport.

Paula M Miotto, Graham P Holloway

Journal article  |  2016  |  Biochemical Journal

The transport of cytosolic adenosine diphosphate (ADP) into the mitochondria is a major control point in metabolic homeostasis, as..

Combined high-fat-resveratrol diet and RIP140 knockout mice reveal a novel relationship between elevated bone mitochondrial content and compromised bone microarchitecture, bone mineral mass, and bone strength in the tibia.

Paula M Miotto, Scott Frendo-Cumbo, Sandra M Sacco, David C Wright, Wendy E Ward, Graham P Holloway

Journal article  |  2016  |  Molecular Nutrition & Food Research

SCOPE: While resveratrol (RSV) is associated with the prevention of high-fat (HF) diet-induced insulin resistance, the effects on ..

High saturated fat diet alters the lipid composition of triacylglycerol and polar lipids in the femur of dam and offspring rats.

Paula M Miotto, Laura M Castelli, Foyinsola Amoye, Wendy E Ward, Paul J LeBlanc

Journal article  |  2015  |  Lipids

Previous work has shown that dietary lipids alter femur lipid composition. Specifically, we have shown that exposure to high satur..

A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood.

Rebecca EK MacPherson, Laura M Castelli, Paula M Miotto, Scott Frendo-Cumbo, Amanda Milburn, Brian D Roy, Paul J LeBlanc, Wendy E Ward, Sandra J Peters

Journal article  |  2015  |  Lipids

A maternal high fat diet (HFD) can have adverse effects on skeletal muscle development. Skeletal muscle PLIN proteins (PLIN2, 3 an..

Maternal high fat feeding does not have long-lasting effects on body composition and bone health in female and male Wistar rat offspring at young adulthood.

Paula M Miotto, Laura M Castelli, Foyinsola Amoye, Paul J LeBlanc, Sandra J Peters, Brian D Roy, Wendy E Ward

Journal article  |  2013  |  Molecules

High fat diets adversely affect body composition, bone mineral and strength, and alter bone fatty acid composition. It is unclear ..