Lachlan Coin

Insights into population structure of East African sweetpotato cultivars from hybrid assembly of chloroplast genomes

Chenxi Zhou, Tania Duarte, Rocio Silvestre, Genoveva Rossel, Robert Mwanga, Awais Khan, Andrew George, Zhangjun Fei, Craig Yencho, David Ellis, Lachlan Coin

Journal article  |  2020  |  Gates Open Research

Background: The chloroplast (cp) genome is an important resource for studying plant diversity and phylogeny. Assembly of the cp ge..

Complete Genome Sequences of Clinical Pandoraea fibrosis Isolates

Miranda E Pitt, Son H Nguyen, Tania PS Duarte, Louise F Roddam, Mark AT Blaskovich, Matthew A Cooper, Lachlan JM Coin

Journal article  |  2020  |  Microbiology Resource Announcements

Pandoraea fibrosis is a newly identified Gram-negative bacterial species that was isolated from the respiratory tract of an Austra..

Real-time resolution of short-read assembly graph using ONT long reads

Son Hoang Nguyen, Minh Duc Cao, Lachlan Coin

Journal article  |  2020

Abstract A streaming assembly pipeline utilising real-time Oxford Nanopore Technology (ONT) sequencing data is important for savin..

Evaluating the genome and resistome of extensively drug-resistant Klebsiella pneumoniae using native DNA and RNA Nanopore sequencing

Miranda E Pitt, Son H Nguyen, Tania PS Duarte, Haotian Teng, Mark AT Blaskovich, Matthew A Cooper, Lachlan JM Coin

Journal article  |  2020  |  GigaScience

BACKGROUND: Klebsiella pneumoniae frequently harbours multidrug resistance, and current diagnostics struggle to rapidly identify a..

Nanopore sequencing as a scalable, cost-effective platform for analyzing polyclonal vector integration sites following clinical T cell therapy

Ping Zhang, Devika Ganesamoorthy, Son Hoang Nguyen, Raymond Au, Lachlan J Coin, Siok-Keen Tey

Journal article  |  2020  |  Journal for ImmunoTherapy of Cancer

BACKGROUND: Analysis of vector integration sites in gene-modified cells can provide critical information on clonality and potentia..

Quantitative trait loci and differential gene expression analyses reveal the genetic basis for negatively associated β-carotene and starch content in hexaploid sweetpotato [Ipomoea batatas (L.) Lam.].

Dorcus C Gemenet, Guilherme da Silva Pereira, Bert De Boeck, Joshua C Wood, Marcelo Mollinari, Bode A Olukolu, Federico Diaz, Veronica Mosquera, Reuben T Ssali, Maria David, Mercy N Kitavi, Gabriela Burgos, Thomas Zum Felde, Marc Ghislain, Edward Carey, Jolien Swanckaert, Lachlan JM Coin, Zhangjun Fei, John P Hamilton, Benard Yada, et al.

Journal article  |  2020  |  Theoretical and Applied Genetics: international journal of plant breeding research

KEY MESSAGE: β-Carotene content in sweetpotato is associated with the Orange and phytoene synthase genes; due to physical linkage ..

Retooling phage display with electrohydrodynamic nanomixing and nanopore sequencing.

Lyndon J Raftery, Christopher B Howard, Yadveer S Grewal, Ramanathan Vaidyanathan, Martina L Jones, Will Anderson, Darren Korbie, Tania Duarte, Minh Duc Cao, Son Hoang Nguyen, Lachlan JM Coin, Stephen M Mahler, Matt Trau

Journal article  |  2019  |  Lab on a Chip

Phage display methodologies offer a versatile platform for the isolation of single-chain Fv (scFv) molecules which may be rebuilt ..

Genotype-free demultiplexing of pooled single-cell RNA-seq.

Jun Xu, Caitlin Falconer, Quan Nguyen, Joanna Crawford, Brett D McKinnon, Sally Mortlock, Anne Senabouth, Stacey Andersen, Han Sheng Chiu, Longda Jiang, Nathan J Palpant, Jian Yang, Michael D Mueller, Alex W Hewitt, Alice Pébay, Grant W Montgomery, Joseph E Powell, Lachlan JM Coin

Journal article  |  2019  |  Genome Biol

A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequencing (scRNA-seq) experiment whic..

Plasma lipid profiles discriminate bacterial from viral infection in febrile children.

Xinzhu Wang, Ruud Nijman, Stephane Camuzeaux, Caroline Sands, Heather Jackson, Myrsini Kaforou, Marieke Emonts, Jethro A Herberg, Ian Maconochie, Enitan D Carrol, Stephane C Paulus, Werner Zenz, Michiel Van der Flier, Ronald de Groot, Federico Martinon-Torres, Luregn J Schlapbach, Andrew J Pollard, Colin Fink, Taco T Kuijpers, Suzanne Anderson, et al.

Journal article  |  2019  |  Scientific Reports

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacteria..

Analysis of polyclonal vector integration sites using Nanopore sequencing as a scalable, cost-effective platform

Ping Zhang, Devika Ganesamoorthy, Son Hoang Nguyen, Raymond Au, Lachlan Coin, Siok-Keen Tey

Journal article  |  2019

Abstract Vector integration site analysis can be important in the follow-up of patients who received gene-modified cells, but curr..