Alisa Glukhova

Automatic local resolution-based sharpening of cryo-EM maps.

Erney Ramírez-Aportela, Jose Luis Vilas, Alisa Glukhova, Roberto Melero, Pablo Conesa, Marta Martínez, David Maluenda, Javier Mota, Amaya Jiménez, Javier Vargas, Roberto Marabini, Patrick M Sexton, Jose Maria Carazo, Carlos Oscar S Sorzano

Journal article  |  2020  |  Bioinformatics

MOTIVATION: Recent technological advances and computational developments have allowed the reconstruction of Cryo-Electron Microsco..

Perturbation of the interactions of calmodulin with GRK5 using a natural product chemical probe.

Tyler S Beyett, Amy E Fraley, Emily Labudde, Dhabaleswar Patra, Ryan C Coleman, Akito Eguchi, Alisa Glukhova, Qiuyan Chen, Robert M Williams, Walter J Koch, David H Sherman, John JG Tesmer

Journal article  |  2019  |  Proceedings of the National Academy of Sciences

G protein-coupled receptor (GPCR) kinases (GRKs) are responsible for initiating desensitization of activated GPCRs. GRK5 is potent..

Molecular basis activation of lecithin: cholesterol acyltransferase by a compound that increases HDL cholesterol

Kelly A Manthei, Shyh-Ming Yang, Bolormaa Baljinnyam, Louise Chang, Alisa Glukhova, Wenmin Yuan, Lita A Freeman, David J Maloney, Anna Schwendeman, Alan T Remaley, Ajit Jadhav, John JG Tesmer

Journal article  |  2018  |  eLife

Rules of Engagement: GPCRs and G Proteins.

Alisa Glukhova, Christopher J Draper-Joyce, Roger K Sunahara, Arthur Christopoulos, Denise Wootten, Patrick M Sexton

Journal article  |  2018  |  ACS Pharmacology & Translational Science

G protein-coupled receptors (GPCRs) are a key drug target class. They account for over one-third of current pharmaceuticals, and b..

Cryo-EM structure of the active, G(s)- protein complexed, human CGRP receptor

Yi-Lynn Liang, Maryam Khoshouei, Giuseppe Deganutti, Alisa Glukhova, Cassandra Koole, Thomas S Peat, Mazdak Radjainia, Jurgen M Plitzko, Wolfgang Baumeister, Laurence J Miller, Deborah L Hay, Arthur Christopoulos, Christopher A Reynolds, Denise Wootten, Patrick M Sexton

Journal article  |  2018  |  Nature

Dominant Negative G Proteins Enhance Formation and Purification of Agonist-GPCR-G Protein Complexes for Structure Determination.

Yi-Lynn Liang, Peishen Zhao, Christopher Draper-Joyce, Jo-Anne Baltos, Alisa Glukhova, Tin T Truong, Lauren T May, Arthur Christopoulos, Denise Wootten, Patrick M Sexton, Sebastian GB Furness

Journal article  |  2018  |  ACS Pharmacology & Translational Science

Advances in structural biology have yielded exponential growth in G protein-coupled receptor (GPCR) structure solution. Nonetheles..

Recent advances in the determination of G protein-coupled receptor structures

David M Thal, Ziva Vuckovic, Christopher J Draper-Joyce, Yi-Lynn Liang, Alisa Glukhova, Arthur Christopoulos, Patrick M Sexton

Journal article  |  2018  |  Current Opinion in Structural Biology

G protein-coupled receptors (GPCRs) are the largest superfamily of cell surface receptor proteins and are important drug targets f..

Structural insights into G-protein-coupled receptor allostery

David M Thal, Alisa Glukhova, Patrick M Sexton, Arthur Christopoulos

Journal article  |  2018  |  Nature

Structure of the adenosine-bound human adenosine A(1) receptor-G(i) complex

Christopher J Draper-Joyce, Maryam Khoshouei, David M Thal, Yi-Lynn Liang, Anh TN Nguyen, Sebastian GB Furness, Hariprasad Venugopal, Jo-Anne Baltos, Juergen M Plitzko, Radostin Danev, Wolfgang Baumeister, Lauren T May, Denise Wootten, Patrick M Sexton, Alisa Glukhova, Arthur Christopoulos

Journal article  |  2018  |  Nature

The class A adenosine A1 receptor (A1R) is a G-protein-coupled receptor that preferentially couples to inhibitory Gi/o heterotrime..

Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex

Yi-Lynn Liang, Maryam Khoshouei, Alisa Glukhova, Sebastian GB Furness, Peishen Zhao, Lachlan Clydesdale, Cassandra Koole, Tin T Truong, David M Thal, Saifei Lei, Mazdak Radjainia, Radostin Danev, Wolfgang Baumeister, Ming-Wei Wang, Laurence J Miller, Arthur Christopoulos, Patrick M Sexton, Denise Wootten

Journal article  |  2018  |  Nature

A retractable lid in lecithin:cholesterol acyltransferase provides a structural mechanism for activation by apolipoprotein A-I

Kelly A Manthei, Joomi Ahn, Alisa Glukhova, Wenmin Yuan, Christopher Larkin, Taylor D Manett, Louise Chang, James A Shayman, Milton J Axley, Anna Schwendeman, John JG Tesmer

Journal article  |  2017  |  Journal of Biological Chemistry

Phase-plate cryo-EM structure of a class B GPCR-G-protein complex

Yi-Lynn Liang, Maryam Khoshouei, Mazdak Radjainia, Yan Zhang, Alisa Glukhova, Jeffrey Tarrasch, David M Thal, Sebastian GB Furness, George Christopoulos, Thomas Coudrat, Radostin Danev, Wolfgang Baumeister, Laurence J Miller, Arthur Christopoulos, Brian K Kobilka, Denise Wootten, Georgios Skiniotis, Patrick M Sexton

Journal article  |  2017  |  Nature

Structure of the Adenosine A(1) Receptor Reveals the Basis for Subtype Selectivity

Alisa Glukhova, David M Thal, Anh T Nguyen, Elizabeth A Vecchio, Manuela Jorg, Peter J Scammells, Lauren T May, Patrick M Sexton, Arthur Christopoulos

Journal article  |  2017  |  Cell

The adenosine A1 receptor (A1-AR) is a G-protein-coupled receptor that plays a vital role in cardiac, renal, and neuronal processe..

Novel Irreversible Agonists Acting at the A(1) Adenosine Receptor

Manuela Jorg, Alisa Glukhova, Alaa Abdul-Ridha, Elizabeth A Vecchio, Anh TN Nguyen, Patrick M Sexton, Paul J White, Lauren T May, Arthur Christopoulos, Peter J Scammells

Journal article  |  2016  |  Journal of Medicinal Chemistry

The A1 adenosine receptor (A1AR) is an important G protein-coupled receptor that regulates a range of physiological functions. Her..

Crystal Structure of G Protein-coupled Receptor Kinase 5 in Complex with a Rationally Designed Inhibitor

Kristoff T Homan, Helen V Waldschmidt, Alisa Glukhova, Alessandro Cannavo, Jianliang Song, Joseph Y Cheung, Walter J Koch, Scott D Larsen, John JG Tesmer

Journal article  |  2015  |  Journal of Biological Chemistry

G protein-coupled receptor kinases (GRKs) regulate cell signaling by initiating the desensitization of active G protein-coupled re..

Structure and function of lysosomal phospholipase A2 and lecithin: cholesterol acyltransferase

Alisa Glukhova, Vania Hinkovska-Galcheva, Robert Kelly, Akira Abe, James A Shayman, John JG Tesmer

Journal article  |  2015  |  Nature Communications

Lysosomal phospholipase A2 (LPLA2) and lecithin:cholesterol acyltransferase (LCAT) belong to a structurally uncharacterized family..

Architecture of the Nitric-oxide Synthase Holoenzyme Reveals Large Conformational Changes and a Calmodulin-driven Release of the FMN Domain

Adam L Yokom, Yoshihiro Morishima, Miranda Lau, Min Su, Alisa Glukhova, Yoichi Osawa, Daniel R Southworth

Journal article  |  2014  |  Journal of Biological Chemistry

Unveiling the Membrane-Binding Properties of N-Terminal and C-Terminal Regions of G Protein-Coupled Receptor Kinase 5 by Combined Optical Spectroscopies

Bei Ding, Alisa Glukhova, Katarzyna Sobczyk-Kojiro, Henry I Mosberg, John JG Tesmer, Zhan Chen

Journal article  |  2014  |  Langmuir

Membrane Orientation and Binding Determinants of G Protein-Coupled Receptor Kinase 5 as Assessed by Combined Vibrational Spectroscopic Studies

Pei Yang, Alisa Glukhova, John JG Tesmer, Zhan Chen

Journal article  |  2013  |  PLoS ONE

Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding

Sergey A Vishnivetskiy, Martin K Ostermaier, Ankita Singhal, Valerie Panneels, Kristoff T Homan, Alisa Glukhova, Stephen G Sligar, John JG Tesmer, Gebhard FX Schertler, Joerg Standfuss, Vsevolod V Gurevich

Journal article  |  2013  |  Cellular Signalling

Regulation of G Protein-Coupled Receptor Kinases by Phospholipids

KT Homan, A Glukhova, JJG Tesmer

Journal article  |  2013  |  Current Medicinal Chemistry

Heterooligomeric complexes formed by human small heat shock proteins HspB1 (Hsp27) and HspB6 (Hsp20)

Olesya V Bukach, Alisa E Glukhova, Alim S Seit-Nebi, Nikolai B Gusev

Journal article  |  2009  |  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics