Megan Maher

Structural and functional characterizations of the C-terminal domains of CzcD proteins

Saumya R Udagedara, Daniel M La Porta, Christian Spehar, Ghruta Purohit, Matthew JA Hein, Monique E Fatmous, G Patricia Casas Garcia, Katherine Ganio, Christopher A McDevitt, Megan J Maher

Journal article  |  2020  |  Journal of Inorganic Biochemistry

Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist ..

Human glutaredoxin-1 can transfer copper to isolated metal binding domains of the P1B-type ATPase, ATP7B.

Shadi Maghool, Sharon La Fontaine, Blaine R Roberts, Ann H Kwan, Megan J Maher

Journal article  |  2020  |  Scientific Reports

Intracellular copper (Cu) in eukaryotic organisms is regulated by homeostatic systems, which rely on the activities of soluble met..

Structural and functional characterization of the mitochondrial complex IV assembly factor Coa6

Shadi Maghool, N Dinesha G Cooray, David A Stroud, David Aragao, Michael T Ryan, Megan J Maher

Journal article  |  2019  |  Life Science Alliance

Assembly factors play key roles in the biogenesis of many multi-subunit protein complexes regulating their stability, activity, an..

The purification of the sigma(FPvI)/FpvR(20) and sigma(Pvds)/FpvR(20) protein complexes is facilitated at room temperature

G Patricia Casas Garcia, Matthew A Perugini, Lain L Lamont, Megan J Maher

Journal article  |  2019  |  Protein Expression and Purification

Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus

Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King, et al.

Journal article  |  2019  |  NATURE COMMUNICATIONS

The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family

Saumya R Udagedara, Chathuri JK Wijekoon, Zhiguang Xiao, Anthony G Wedd, Megan J Maher

Journal article  |  2019  |  Journal of Inorganic Biochemistry

High-resolution crystal structure of the reduced Grx1 from Saccharomyces cerevisiae.

Shadi Maghool, Sharon La Fontaine, Megan J Maher

Journal article  |  2019  |  Acta Crystallographica Section F Structural Biology Communications

Grx1, a cytosolic thiol-disulfide oxidoreductase, actively maintains cellular redox homeostasis using glutathione substrates (redu..

The structure and activity of the glutathione reductase from Streptococcus pneumoniae

Mwilye Sikanyika, David Aragao, Christopher A McDevitt, Megan J Maher

Journal article  |  2019  |  Acta Crystallographica Section F Structural Biology Communications

Crystal structure of bacterial succinate: quinone oxidoreductase flavoprotein SdhA in complex with its assembly factor SdhE

Megan J Maher, Anuradha S Herath, Saumya R Udagedara, David A Dougan, Kaye N Truscott

Journal article  |  2018  |  Proceedings of the National Academy of Sciences

The central active site arginine in sulfite oxidizing enzymes alters kinetic properties by controlling electron transfer and redox interactions

Ju-Chun Hsiao, Aaron P McGrath, Linda Kielmann, Palraj Kalimuthu, Farzana Darain, Paul V Bernhardt, Jeffrey Harmer, Mihwa Lee, Kimberley Meyers, Megan J Maher, Ulrike Kappler

Journal article  |  2018  |  Biochimica et Biophysica Acta (BBA) - Bioenergetics

The crystal structures of a copper-bound metallochaperone from Saccharomyces cerevisiae

Mihwa Lee, N Dinesha G Cooray, Megan J Maher

Journal article  |  2017  |  Journal of Inorganic Biochemistry

Integrated activities of two alternative sigma factors coordinate iron acquisition and uptake by Pseudomonas aeruginosa

Rebecca J Edgar, Georgina E Hampton, G Patricia Casas Garcia, Megan J Maher, Matthew A Perugini, David F Ackerley, Iain L Lamont

Journal article  |  2017  |  Molecular Microbiology

Structural basis of interprotein electron transfer in bacterial sulfite oxidation

Aaron P McGrath, Elise L Laming, G Patricia Casas Garcia, Marc Kvansakul, J Mitchell Guss, Jill Trewhella, Benoit Calmes, Paul V Bernhardt, Graeme R Hanson, Ulrike Kappler, Megan J Maher

Journal article  |  2015  |  eLife

COA6 is a mitochondrial complex IV assembly factor critical for biogenesis of mtDNA-encoded COX2

David A Stroud, Megan J Maher, Caroline Lindau, F-Nora Voegtle, Ann E Frazier, Elliot Surgenor, Hayley Mountford, Abeer P Singh, Matteo Bonas, Silke Oeljeklaus, Bettina Warscheid, Chris Meisinger, David R Thorburn, Michael T Ryan

Journal article  |  2015  |  Human Molecular Genetics

Dysregulation of transition metal ion homeostasis is the molecular basis for cadmium toxicity in Streptococcus pneumoniae

Stephanie L Begg, Bart A Eijkelkamp, Zhenyao Luo, Rafael M Counago, Jacqueline R Morey, Megan J Maher, Cheryl-lynn Y Ong, Alastair G McEwan, Bostjan Kobe, Megan L O'Mara, James C Paton, Christopher A McDevitt

Journal article  |  2015  |  NATURE COMMUNICATIONS

A GTPase Chimera Illustrates an Uncoupled Nucleotide Affinity and Release Rate, Providing Insight into the Activation Mechanism

Amy P Guilfoyle, Chandrika N Deshpande, Josep Font Sadurni, Miriam-Rose Ash, Samuel Tourle, Gerhard Schenk, Megan J Maher, Mika Jormakka

Journal article  |  2014  |  Biophysical Journal

Structural and functional analysis of a FeoB A143S G5 loop mutant explains the accelerated GDP release rate

Amy P Guilfoyle, Chandrika N Deshpande, Kimberley Vincent, Marcelo M Pedroso, Gerhard Schenk, Megan J Maher, Mika Jormakka

Journal article  |  2014  |  FEBS Journal

The magic of molybdenum

M Maher

Conference Proceedings  |  2014  |  Australian Life Scientist

Exploring the correlation between the sequence composition of the nucleotide binding G5 loop of the FeoB GTPase domain (NFeoB) and intrinsic rate of GDP release

Amy P Guilfoyle, Chandrika N Deshpande, Gerhard Schenk, Megan J Maher, Mika Jormakka

Journal article  |  2014  |  Bioscience Reports

Structure of an atypical FeoB G-domain reveals a putative domain-swapped dimer

Chandrika N Deshpande, Aaron P McGrath, Josep Font, Amy P Guilfoyle, Megan J Maher, Mika Jormakka

Journal article  |  2013  |  Acta Crystallographica Section F Structural Biology and Crystallization Communications

The bacterial SoxAX cytochromes

Ulrike Kappler, Megan J Maher

Journal article  |  2013  |  Cellular and Molecular Life Sciences

The X-ray crystal structure of a pseudoazurin from Sinorhizobium meliloti

Elise M Laming, Aaron P McGrath, J Mitchell Guss, Ulrike Kappler, Megan J Maher

Journal article  |  2012  |  Journal of Inorganic Biochemistry

The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates

Momi Iwata, Yang Lee, Tetsuo Yamashita, Takao Yagi, So Iwata, Alexander D Cameron, Megan J Maher

Journal article  |  2012  |  Proceedings of the National Academy of Sciences

The cation-dependent G-proteins: In a class of their own

Miriam-Rose Ash, Megan J Maher, J Mitchell Guss, Mika Jormakka

Journal article  |  2012  |  FEBS Letters

The structure of an N11A mutant of the G-protein domain of FeoB

Miriam-Rose Ash, Megan J Maher, J Mitchell Guss, Mika Jormakka

Journal article  |  2011  |  Acta Crystallographica Section F Structural Biology and Crystallization Communications

Molecular Basis of the Cooperative Binding of Cu(I) and Cu(II) to the CopK Protein from Cupriavidus metallidurans CH34

Miriam-Rose Ash, Lee Xin Chong, Megan J Maher, Mark G Hinds, Zhiguang Xiao, Anthony G Wedd

Journal article  |  2011  |  Biochemistry

A suite of Switch I and Switch II mutant structures from the G-protein domain of FeoB

Miriam-Rose Ash, Megan J Maher, J Mitchell Guss, Mika Jormakka

Journal article  |  2011  |  Acta Crystallographica Section D Biological Crystallography

The acquisition of ferrous iron in prokaryotes is achieved by the G-protein-coupled membrane protein FeoB. This protein possesses ..

The Initiation of GTP Hydrolysis by the G-Domain of FeoB: Insights from a Transition-State Complex Structure

Miriam-Rose Ash, Megan J Maher, J Mitchell Guss, Mika Jormakka

Journal article  |  2011  |  PLoS ONE

Insights into Structure and Function of the Active Site of SoxAX Cytochromes

James R Kilmartin, Megan J Maher, Kuakarun Krusong, Christopher J Noble, Graeme R Hanson, Paul V Bernhardt, Mark J Riley, Ulrike Kappler

Journal article  |  2011  |  Journal of Biological Chemistry

Potassium-activated GTPase Reaction in the G Protein-coupled Ferrous Iron Transporter B

Miriam-Rose Ash, Amy Guilfoyle, Ronald J Clarke, Mitchell Guss, Megan J Maher, Mika Jormakka

Journal article  |  2010  |  Journal of Biological Chemistry

Crystal structure of A(3)B(3) complex of V-ATPase from Thermus thermophilus

Megan J Maher, Satoru Akimoto, Momi Iwata, Koji Nagata, Yoshiko Hori, Masasuke Yoshida, Shigeyuki Yokoyama, So Iwata, Ken Yokoyama

Journal article  |  2009  |  The EMBO Journal

Dihydroorotase Inhibitors

M Lee, MJ Maher, RI Christopherson, JM Guss

Book Chapter  |  2009

Structural basis of GDP release and gating in G protein coupled Fe2 transport

Amy Guilfoyle, Megan J Maher, Mikaela Rapp, Ronald Clarke, Stephen Harrop, Mika Jormakka

Journal article  |  2009  |  The EMBO Journal

Crystal Structure of the Acid-Induced Arginine Decarboxylase from Escherichia coli: Reversible Decamer Assembly Controls Enzyme Activity

Juni Andrell, Matthew G Hicks, Tracy Palmer, Elisabeth P Carpenter, So Iwata, Megan J Maher

Journal article  |  2009  |  Biochemistry

Unprecedented Binding Cooperativity between Cu-I and Cu-II in the Copper Resistance Protein CopK from Cupriavidus metallidurans CH34: Implications from Structural Studies by NMR Spectroscopy and X-Ray Crystallography

Lee Xin Chong, Miriam-Rose Ash, Megan J Maher, Mark G Hinds, Zhiguang Xiao, Anthony G Wedd

Journal article  |  2009  |  Journal of the American Chemical Society

Thioredoxin A active-site mutants form mixed disulfide dimers that resemble enzyme-substrate reaction intermediates

Thijs RHM Kouwen, Jun Andrell, Rianne Schrijver, Jean-Yves F Dubois, Megan J Maher, So Iwata, Elisabeth P Carpenter, Jan Maarten van Dijl

Journal article  |  2008  |  Journal of Molecular Biology

Kinetic and structural analysis of mutant Escherichia coli dihydroorotases: A flexible loop stabilizes the transition state

Mihwa Lee, Megan J Maher, Richard I Christopherson, J Mitchell Guss

Journal article  |  2007  |  Biochemistry

Structures of ligand-free and inhibitor complexes of Dihydroorotase from Escherichia coli: Implications for loop movement in inhibitor design

Mihwa Lee, Camilla W Chan, Stephen C Graham, Richard I Christopherson, J Mitchell Guss, Megan J Maher

Journal article  |  2007  |  Journal of Molecular Biology

Structure of the T109S mutant of Escherichia coli dihydroorotase complexed with the inhibitor 5-fluoroorotate: catalytic activity is reflected by the crystal form

Mihwa Lee, Megan J Maher, J Mitchell Guss

Journal article  |  2007  |  Acta Crystallographica Section F Structural Biology and Crystallization Communications

Intermolecular transfer of copper ions from the CopC protein of Pseudomonas syringae. Crystal structures of fully loaded (CuCuII)-Cu-I forms

L Zhang, M Koay, MJ Mahert, Z Xiao, AG Wedd

Journal article  |  2006  |  Journal of the American Chemical Society

CopC protein from Pseudomonas syringae: Intermolecular transfer of copper from both the copper(I) and copper(II) sites

M Koay, LY Zhang, BS Yang, MJ Maher, ZG Xiao, AG Wedd

Journal article  |  2005  |  Inorganic Chemistry

Dihydroorotase from Escherichia coli: Loop movement and cooperativity between subunits

M Lee, CW Chan, JM Guss, RI Christopherson, MJ Maher

Journal article  |  2005  |  Journal of Molecular Biology

Structure of Escherichia coli aminopeptidase P in complex with the inhibitor apstatin

SC Graham, MJ Maher, WH Simmons, HC Freeman, JM Guss

Journal article  |  2004  |  Acta Crystallographica Section D Biological Crystallography

Tandem LIM domains provide synergistic binding in the LMO4 : Ldb1 complex

JE Deane, DP Ryan, M Sunde, MJ Maher, JM Guss, JE Visvader, JM Matthews

Journal article  |  2004  |  The EMBO Journal

Metal-substituted derivatives of the rubredoxin from Clostridium pasteurianum (vol D60, pg 298, 2004)

M Maher, M Cross, MCJ Wilce, JM Guss, AG Wedd

Journal article  |  2004  |  Acta Crystallographica Section D Biological Crystallography

Structure of the prolidase from Pyrococcus furiosus

MJ Maher, M Ghosh, AM Grunden, AL Menon, MWW Adams, HC Freeman, JM Guss

Journal article  |  2004  |  Biochemistry

Metal-substituted derivatives of the rubredoxin from Clostridium pasteurianum

M Maher, M Cross, MCJ Wilce, JM Guss, AG Wedd

Journal article  |  2004  |  Acta Crystallographica Section D Biological Crystallography

X-ray absorption spectroscopy of selenate reductase

MJ Maher, J Santini, IJ Pickering, RC Prince, JM Macy, GN George

Journal article  |  2004  |  Inorganic Chemistry

Auracyanin B structure in space group P6(5)

M Lee, MJ Maher, HC Freeman, JM Guss

Journal article  |  2003  |  Acta Crystallographica Section D Biological Crystallography

Crystallization of FLINC4, an intramolecular LMO4-ldb1 complex

JE Deane, MJ Maher, DB Langley, SC Graham, JE Visvader, JM Guss, JM Matthews

Journal article  |  2003  |  Acta Crystallographica Section D Biological Crystallography

Structural studies on the selenate reductase from Thauera selenatis.

MJ Maher, JM Santini, GN George

Conference Proceedings  |  2003  |  Journal of Inorganic Biochemistry

Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form

MJ Maher, DTC Huang, JM Guss, CA Collyer, RI Christopherson

Journal article  |  2003  |  Acta Crystallographica Section D Biological Crystallography

Crystallization of Pichia pastoris lysyl oxidase

M Lee, K Willingham, D Langley, MJ Maher, AE Cohen, PJ Ellis, JA Kuchar, DM Dooley, HC Freeman, JM Guss

Journal article  |  2002  |  Acta Crystallographica Section D Biological Crystallography

Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis

MJ Maher, JM Macy

Journal article  |  2002  |  Acta Crystallographica Section D Biological Crystallography

Crystallization and characterization of the prolidase from Pyrococcus furiosus

K Willingham, MJ Maher, AM Grunden, M Ghosh, MWW Adams, HC Freeman, JM Guss

Journal article  |  2001  |  Acta Crystallographica Section D Biological Crystallography

Crystal structure of auracyanin, a "blue" copper protein from the green thermophilic photosynthetic bacterium Chloroflexus aurantiacus

CS Bond, RE Blankenship, HC Freeman, JM Guss, MJ Maher, FM Selvaraj, MCJ Wilce, KM Willingham

Journal article  |  2001  |  Journal of Molecular Biology

Mutation of the surface valine residues 8 and 44 in the rubredoxin from Clostridium pasteurianum: solvent access versus structural changes as determinants of reversible potential

ZG Xiao, MJ Maher, M Cross, CS Bond, JM Guss, AG Wedd

Journal article  |  2000  |  JBIC Journal of Biological Inorganic Chemistry

The dependence of reversible potentials on the form of modification of edge plane pyrolytic graphite electrodes in voltammetric studies on rubredoxin and ferredoxin from Clostridium pasteurianum

ZG Xiao, MJ Lavery, AM Bond, AG Wedd

Journal article  |  1999  |  Electrochemistry Communications

Rubredoxin from Clostridium pasteurianum. Structures of G10A, G43A and C10VG43A mutant proteins. Mutation of conserved glycine 10 to valine causes the 9-10 peptide link to invert

MJ Maher, ZG Xiao, MCJ Wilce, JM Guss, AG Wedd

Journal article  |  1999  |  Acta Crystallographica Section D Biological Crystallography

Investigation of the role of surface residues in the ferredoxin from Clostridium pasteurianum

PS Brereton, MJ Maher, PA Tregloan, AG Wedd

Journal article  |  1999  |  Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

The rubredoxin from Clostridium pasteurianum: Mutation of the iron cysteinyl ligands to serine. Crystal and molecular structures of oxidized and dithionite-treated forms of the Cys42Ser mutant

ZG Xiao, MJ Lavery, M Ayhan, SDB Scrofani, MCJ Wilce, JM Guss, PA Tregloan, GN George, AG Wedd

Journal article  |  1998  |  Journal of the American Chemical Society

The rubredoxin from Clostridium pasteurianum: Mutation of the conserved glycine residues 10 and 43 to alanine and valine

M Ayhan, ZG Xiao, MJ Lavery, AM Hamer, KW Nugent, SDB Scrofani, M Guss, AG Wedd

Journal article  |  1996  |  Inorganic Chemistry

Rubredoxin from Clostridium pasteurianum: Mutation of the conserved cysteine and glycine residues

M Ayhan, ZG Xiao, MJ Lavery, AM Hammer, SDB Scrofani, AG Wedd

Conference Proceedings  |  1996  |  TRANSITION METAL SULFUR CHEMISTRY

COMPARISON OF NATIVE AND MUTANT PROTEINS PROVIDES A SEQUENCE-SPECIFIC ASSIGNMENT OF THE CYSTEINYL LIGAND PROTON NMR RESONANCES IN THE 2[FE4S4] FERREDOXIN FROM CLOSTRIDIUM-PASTEURIANUM

SDB SCROFANI, PS BRERETON, AM HAMER, MJ LAVERY, SG MCDOWALL, GA VINCENT, RTC BROWNLEE, NJ HOOGENRAAD, M SADEK, AG WEDD

Journal article  |  1994  |  Biochemistry

A sequence-specific assignment is presented for the eight low-field paramagnetically shifted cysteinyl ligand proton NMR resonance..