Melanie Eckersley-Maslin

Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes

Christina E Sparbier, Andrea Gillespie, Juliana Gomez, Nishi Kumari, Ali Motazedian, Kah Lok Chan, Charles C Bell, Omer Gilan, Yih-Chih Chan, Sarah Popp, Daniel J Gough, Melanie A Eckersley-Maslin, Sarah-Jane Dawson, Paul J Lehner, Kate D Sutherland, Patricia Ernst, Gerard M McGeehan, Enid YN Lam, Marian L Burr, Mark A Dawson

Journal article  |  2023  |  NATURE CELL BIOLOGY

Precise control of activating H3K4me3 and repressive H3K27me3 histone modifications at bivalent promoters is essential for normal ..

Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation

Maria Arez, Melanie Eckersley-Maslin, Tajda Klobucar, Joao von Gilsa Lopes, Felix Krueger, Annalisa Mupo, Ana Claudia Raposo, David Oxley, Samantha Mancino, Anne-Valerie Gendrel, Bruno Bernardes de Jesus, Simao Teixeira da Rocha

Journal article  |  2022  |  NATURE COMMUNICATIONS

Reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs) is a major leap towards personalised approaches to dise..

Maternal Dppa2 and Dppa4 are dispensable for zygotic genome activation but important for offspring survival

Oana Kubinyecz, Fatima Santos, Deborah Drage, Wolf Reik, Melanie A Eckersley-Maslin

Journal article  |  2021  |  DEVELOPMENT

Zygotic genome activation (ZGA) represents the initiation of transcription following fertilisation. Despite its importance, we kno..

Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells.

Celia Alda-Catalinas, Melanie A Eckersley-Maslin, Wolf Reik

Journal article  |  2021  |  STAR Protoc

CRISPR/Cas9 screens are a powerful approach to identify key regulators of biological processes. By combining pooled CRISPR/Cas9 sc..

Keeping your options open: insights from Dppa2/4 into how epigenetic priming factors promote cell plasticity

Melanie A Eckersley-Maslin

Journal article  |  2020  |  BIOCHEMICAL SOCIETY TRANSACTIONS

The concept of cellular plasticity is particularly apt in early embryonic development, where there is a tug-of-war between the sta..

Hypermethylation and reduced expression of Gtl2, Rian and Mirg at the Dlk1-Dio3 imprinted locus as a marker for poor developmental potential of mouse embryonic stem cells

Maria Schacker, Yi-Han Cheng, Melanie Eckersley-Maslin, Richard Michael Snaith, William Henry Colledge

Journal article  |  2020  |  STEM CELL RESEARCH

Mouse embryonic stem cells (ESCs) have played a crucial role in biomedical research where they can be used to elucidate gene funct..

IMPLICON: an ultra-deep sequencing method to uncover DNA methylation at imprinted regions

Tajda Klobucar, Elisa Kreibich, Felix Krueger, Maria Arez, Duarte Polvora-Brandao, Ferdinand von Meyenn, Simao Teixeira da Rocha, Melanie Eckersley-Maslin

Journal article  |  2020  |  NUCLEIC ACIDS RESEARCH

Genomic imprinting is an epigenetic phenomenon leading to parental allele-specific expression. Dosage of imprinted genes is crucia..

A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program

Celia Alda-Catalinas, Danila Bredikhin, Irene Hernando-Herraez, Fatima Santos, Oana Kubinyecz, Melanie A Eckersley-Maslin, Oliver Stegle, Wolf Reik

Journal article  |  2020  |  CELL SYSTEMS

Zygotic genome activation (ZGA) is an essential transcriptional event in embryonic development that coincides with extensive epige..

Epigenetic priming by Dppa2 and 4 in pluripotency facilitates multi-lineage commitment

Melanie A Eckersley-Maslin, Aled Parry, Marloes Blotenburg, Christel Krueger, Yoko Ito, Valar Nila Roamio Franklin, Masashi Narita, Clive S D'Santos, Wolf Reik

Journal article  |  2020  |  NATURE STRUCTURAL & MOLECULAR BIOLOGY

How the epigenetic landscape is established in development is still being elucidated. Here, we uncover developmental pluripotency ..

Establishment of porcine and human expanded potential stem cells

Xuefei Gao, Monika Nowak-Imialek, Xi Chen, Dongsheng Chen, Doris Herrmann, Degong Ruan, Andy Chun Hang Chen, Melanie A Eckersley-Maslin, Shakil Ahmad, Yin Lau Lee, Toshihiro Kobayashi, David Ryan, Jixing Zhong, Jiacheng Zhu, Jian Wu, Guocheng Lan, Stoyan Petkov, Jian Yang, Liliana Antunes, Lia S Campos, et al.

Journal article  |  2019  |  NATURE CELL BIOLOGY

We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular p..