The lipidomics of cell fate
Grant number: FT140100047 | Funding period: 2015 - 2018
This project aims to dissect the roles of lipids in cell fate. The study of lipids, or lipidomics, is an emerging and exciting area of biological science. The fundamental roles of lipids in development remain vastly understudied. This project will look at reprogramming of somatic cells into stem cells, their pluripotency and differentiation. This will be complemented with studies in the zebrafish, which permits the direct study of cell fate in vivo. This approach is a powerful way to unlock major events involved in development and to unmask the roles of lipids in these fundamental mechanisms.
Related publications (34)
Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time
Grace E Lidgerwood, Anne Senabouth, Casey JA Smith-Anttila, Vikkitharan Gnanasambandapillai, Dominik C Kaczorowski, Daniela Amann-Zalcenstein, Erica L Fletcher, Shalin H Naik, Alex W Hewitt, Joseph E Powell, Alice Pebay
Human pluripotent stem cell (hPSC)-derived progenies are immature versions of cells, presenting a potential limitation to the accu..
Single cell eQTL analysis identifies cell type-specific genetic control of gene expression in fibroblasts and reprogrammed induced pluripotent stem cells
Drew Neavin, Quan Nguyen, Maciej S Daniszewski, Helena H Liang, Han Sheng Chiu, Yong Kiat Wee, Anne Senabouth, Samuel W Lukowski, Duncan E Crombie, Grace E Lidgerwood, Damian Hernandez, James C Vickers, Anthony L Cook, Nathan J Palpant, Alice Pebay, Alex W Hewitt, Joseph E Powell
BACKGROUND: The discovery that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) has provided a foundati..
If Human Brain Organoids Are the Answer to Understanding Dementia, What Are the Questions?
Lezanne Ooi, Mirella Dottori, Anthony L Cook, Martin Engel, Vini Gautam, Alexandra Grubman, Damian Hernandez, Anna E King, Simon Maksour, Helena Targa Dias Anastacio, Rachelle Balez, Alice Pebay, Colin Pouton, Michael Valenzuela, Anthony White, Robert Williamson
Because our beliefs regarding our individuality, autonomy, and personhood are intimately bound up with our brains, there is a publ..
The current state of stem cell therapy for ocular disease
Samantha Bobba, Nick Di Girolamo, Megan Munsie, Fred Chen, Alice Pebay, Damien Harkin, Alex W Hewitt, Michael O'Connor, Samuel McLenachan, Audra MA Shadforth, Stephanie L Watson
Herein, we review the safety, efficacy, regulatory standards and ethical implications of the use of stem cells in ocular disease. ..
Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media
Maciej Daniszewski, Nguyen Quan, Hun S Chy, Vikrant Singh, Duncan E Crombie, Tejal Kulkarni, Helena H Liang, Priyadharshini Sivakumaran, Grace E Lidgerwood, Damien Hernandez, Alison Conquest, Louise A Rooney, Sophie Chevalier, Stacey B Andersen, Anne Senabouth, James C Vickers, David A Mackey, Jamie E Craig, Andrew L Laslett, Alex W Hewitt
We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex an..
Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies
Maciej Daniszewski, Duncan E Crombie, Rachael Henderson, Helena H Liang, Raymond CB Wong, Alex W Hewitt, Alice Pebay
Pluripotent stem cells are an extremely powerful tool in modeling human diseases and hold much promise for personalized regenerati..
Longitudinal expression profiling of CD4 and CD8 cells in patients with active to quiescent giant cell arteritis
Elisabeth De Smit, Samuel W Lukowski, Lisa Anderson, Anne Senabouth, Kaisar Dauyey, Sharon Song, Bruce Wyse, Lawrie Wheeler, Christine Y Chen, Khoa Cao, Amy Wong Ten Yuen, Neil Shuey, Linda Clarke, Isabel Lopez Sanchez, Sandy SC Hung, Alice Pebay, David A Mackey, Matthew A Brown, Alex W Hewitt, Joseph E Powell
BACKGROUND: Giant cell arteritis (GCA) is the most common form of vasculitis affecting elderly people. It is one of the few true o..
Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells
Ashfaqul Hogue, Priyadharshini Sivakumaran, Simon T Bond, Naomi XY Ling, Anne M Kong, John W Scott, Nadeeka Bandara, Damian Hernandez, Guei-Sheung Liu, Raymond CB Wong, Michael T Ryan, Derek J Hausenloy, Bruce E Kemp, Jonathan S Oakhill, Brian G Drew, Alice Pebay, Shiang Y Lim
Human induced pluripotent stem cells (iPSCs) are a valuable tool for studying the cardiac developmental process in vitro, and card..
Single cell RNA sequencing of stem cell-derived retinal ganglion cells
Maciej Brisbane Daniszewski, Anne Senabouth, Quan H Nguyen, Duncan E Crombie, Samuel W Lukowski, Tejal Kulkarni, Valentin M Sluch, Jafar S Jabbari, Xitiz Chamling, Donald J Zack, Alice Pebay, Joseph E Powell, Alex W Hewitt
We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ga..
Biologically active composition of human W8B2( ) cardiac stem cell secretome
P Sivakumaran, S Nie, X Wang, T Karnezis, N Bandara, C Nowell, S Wilcox, AF Hill, M Shambrook, NC Harris, AE Newcomb, P Strappe, R Shayan, D Hernandez, J Clarke, GJ Dusting, K Takov, S Davidson, E Hanssen, A Pebay
Drusen in patient-derived hiPSC-RPE models of macular dystrophies
Chad A Galloway, Sonal Dalvi, Sandy SC Hung, Leslie A MacDonald, Lisa R Latchney, Raymond CB Wong, Robyn H Guymer, David A Mackey, David S Williams, Mina M Chung, David M Gamm, Alice Pebay, Alex W Hewitt, Ruchira Singh
Age-related macular degeneration (AMD) and related macular dystrophies (MDs) are a major cause of vision loss. However, the mechan..
Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency
Duncan E Crombie, Claire L Curl, Antonia JA Raaijmakers, Priyadharshini Sivakumaran, Tejal Kulkarni, Raymond CB Wong, Itsunari Minami, Marguerite V Evans-Galea, Shiang Y Lim, Lea Delbridge, Louise A Corben, Mirella Dottori, Norio Nakatsuji, Ian A Trounce, Alex W Hewitt, Martin B Delatycki, Martin F Pera, Alice Pebay
We sought to identify the impacts of Friedreich's ataxia (FRDA) on cardiomyocytes. FRDA is an autosomal recessive degenerative con..
Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy
Raymond CB Wong, Shiang Y Lim, Sandy SC Hung, Stacey Jackson, Shahnaz Khan, Nicole J Van Bergen, Elisabeth De Smit, Helena H Liang, Lisa S Kearns, Linda Clarke, David A Mackey, Alex W Hewitt, Ian A Trounce, Alice Pebay
Cybrid technology was used to replace Leber hereditary optic neuropathy (LHON) causing mitochondrial DNA (mtDNA) mutations from pa..
Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish
Frisca Frisca, Daniel Colquhoun, Yona Goldshmit, Minna-Liisa Anko, Alice Pebay, Jan Kaslin
Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and orga..
ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy(aEuro)
Dean G Phelan, David J Anderson, Sara E Howden, Raymond CB Wong, Peter F Hickey, Kate Pope, Gabrielle R Wilson, Alice Pebay, Andrew M Davis, Steven Petrou, Andrew G Elefanty, Edouard G Stanley, Paul A James, Ivan Macciocca, Melanie Bahlo, Michael M Cheung, David J Amor, David A Elliott, Paul J Lockhart
AIMS: We identified a novel homozygous truncating mutation in the gene encoding alpha kinase 3 (ALPK3) in a family presenting with..
Participant understanding and recall of informed consent for induced pluripotent stem cell biobanking
Tristan McCaughey, Christine Y Chen, Elisabeth De Smit, Gwyneth Rees, Eva Fenwick, Lisa S Kearns, David A Mackey, Casimir MacGregor, Megan Munsie, Anthony L Cook, Alice Pebay, Alex W Hewitt
The ability to generate human induced pluripotent stem cells (iPSCs) has opened new avenues for human disease modelling and therap..
Enriched retinal ganglion cells derived from human embryonic stem cells
Katherine P Gill, Sandy SC Hung, Alexei Sharov, Camden Y Lo, Karina Needham, Grace E Lidgerwood, Stacey Jackson, Duncan E Crombie, Bryony A Nayagam, Anthony L Cook, Alex W Hewitt, Alice Pebay, Raymond CB Wong
Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cel..
Genome engineering in ophthalmology: Application of CRISPR/Cas to the treatment of eye disease
Sandy SC Hung, Tristan McCaughey, Olivia Swann, Alice Pebay, Alex W Hewitt
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and CRISPR-associated protein (Cas) system has enabled an ac..
Targeted therapeutic mild hypercapnia after cardiac arrest: A phase II multi-centre randomised controlled trial (the CCC trial)
Glenn M Eastwood, Antoine G Schneider, Satoshi Suzuki, Leah Peck, Helen Young, Aiko Tanaka, Johan Martensson, Stephen Warrillow, Shay McGuinness, Rachael Parke, Eileen Gilder, Lianne Mccarthy, Pauline Galt, Gopal Taori, Suzanne Eliott, Tammy Lamac, Michael Bailey, Nerina Harley, Deborah Barge, Carol L Hodgson
BACKGROUND: In intensive care observational studies, hypercapnia after cardiac arrest (CA) is independently associated with improv..
AAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells In Vivo
Sandy SC Hung, Vicki Chrysostomou, Fan Li, Jeremiah KH Lim, Jiang-Hui Wang, Joseph E Powell, Leilei Tu, Maciej Daniszewski, Camden Lo, Raymond C Wong, Jonathan G Crowston, Alice Pebay, Anna E King, Bang V Bui, Guei-Sheung Liu, Alex W Hewitt
PURPOSE: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has recently been adap..
A Global Social Media Survey of Attitudes to Human Genome Editing
Tristan McCaughey, Paul G Sanfilippo, George EC Gooden, David M Budden, Li Fan, Eva Fenwick, Gwyneth Rees, Casimir MacGregor, Lei Si, Christine Chen, Helena Hai Liang, Timothy Baldwin, Alice Pebay, Alex W Hewitt
Ongoing breakthroughs with CRISPR/Cas-based editing could potentially revolutionize modern medicine, but there are many questions ..
Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells
Sandy SC Hung, Nicole J Van Bergen, Stacey Jackson, Helena Liang, David A Mackey, Damian Hernandez, Shiang Y Lim, Alex W Hewitt, Ian Trounce, Alice Pebay, Raymond CB Wong
Reprogramming of somatic cells into a pluripotent state is known to be accompanied by extensive restructuring of mitochondria and ..
Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium
Grace E Lidgerwood, Shiang Y Lim, Duncan E Crombie, Ray Ali, Katherine P Gill, Damian Hernandez, Josh Kie, Alison Conquest, Hayley S Waugh, Raymond CB Wong, Helena H Liang, Alex W Hewitt, Kathryn C Davidson, Alice Pebay
We demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the ..
An Interactive Multimedia Approach to Improving Informed Consent for Induced Pluripotent Stem Cell Research
Tristan McCaughey, Helena H Liang, Christine Chen, Eva Fenwick, Gwyneth Rees, Raymond CB Wong, James C Vickers, Mathew J Summers, Casimir MacGregor, Jamie E Craig, Megan Munsie, Alice Pebay, Alex W Hewitt
Cardiac Repair With a Novel Population of Mesenchymal Stem Cells Resident in the Human Heart
Yuan Zhang, Priyadharshini Sivakumaran, Andrew E Newcomb, Damian Hernandez, Nicole Harris, Ramin Khanabdali, Guei-Sheung Liu, Darren J Kelly, Alice Pebay, Alex W Hewitt, Andrew Boyle, Richard Harvey, Wayne A Morrison, David A Elliott, Gregory J Dusting, Shiang Y Lim
Cardiac resident stem cells (CRSCs) hold much promise to treat heart disease but this remains a controversial field. Here, we desc..
DECREASED ANTI-REGENERATIVE EFFECTS AFTER SPINAL CORD INJURY IN SPRY4-/- MICE
Y Goldshmit, F Frisca, J Kaslin, AR Pinto, J-KKY Tang, A Pebay, R Pinkas-Kramarski, PD Currie
Previously, we have demonstrated a role for fibroblast growth factor (Fgf) in spinal cord regeneration in both zebrafish and mouse..