The lipidomics of cell fate

Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time

Grace E Lidgerwood, Anne Senabouth, Casey JA Smith-Anttila, Vikkitharan Gnanasambandapillai, Dominik C Kaczorowski, Daniela Amann-Zalcenstein, Erica L Fletcher, Shalin H Naik, Alex W Hewitt, Joseph E Powell, Alice Pebay

2021-11-13

Human pluripotent stem cell (hPSC)-derived progenies are immature versions of cells, presenting a potential limitation to the accu..

Single cell eQTL analysis identifies cell type-specific genetic control of gene expression in fibroblasts and reprogrammed induced pluripotent stem cells

Drew Neavin, Quan Nguyen, Maciej S Daniszewski, Helena H Liang, Han Sheng Chiu, Yong Kiat Wee, Anne Senabouth, Samuel W Lukowski, Duncan E Crombie, Grace E Lidgerwood, Damian Hernandez, James C Vickers, Anthony L Cook, Nathan J Palpant, Alice Pebay, Alex W Hewitt, Joseph E Powell

2021-03-05

BACKGROUND: The discovery that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) has provided a foundati..

If Human Brain Organoids Are the Answer to Understanding Dementia, What Are the Questions?

Lezanne Ooi, Mirella Dottori, Anthony L Cook, Martin Engel, Vini Gautam, Alexandra Grubman, Damian Hernandez, Anna E King, Simon Maksour, Helena Targa Dias Anastacio, Rachelle Balez, Alice Pebay, Colin Pouton, Michael Valenzuela, Anthony White, Robert Williamson

2020-10-01

Because our beliefs regarding our individuality, autonomy, and personhood are intimately bound up with our brains, there is a publ..

The current state of stem cell therapy for ocular disease

Samantha Bobba, Nick Di Girolamo, Megan Munsie, Fred Chen, Alice Pebay, Damien Harkin, Alex W Hewitt, Michael O'Connor, Samuel McLenachan, Audra MA Shadforth, Stephanie L Watson

2018-12-01

Herein, we review the safety, efficacy, regulatory standards and ethical implications of the use of stem cells in ocular disease. ..

Roles of lysophosphatidic acid and sphingosine-1-phosphate in stem cell biology

Grace E Lidgerwood, Stuart M Pitson, Claudine Bonder, Alice Pebay

2018-10-01

Stem cells are unique in their ability to self-renew and differentiate into various cell types. Because of these features, stem ce..

Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

Maciej Daniszewski, Nguyen Quan, Hun S Chy, Vikrant Singh, Duncan E Crombie, Tejal Kulkarni, Helena H Liang, Priyadharshini Sivakumaran, Grace E Lidgerwood, Damien Hernandez, Alison Conquest, Louise A Rooney, Sophie Chevalier, Stacey B Andersen, Anne Senabouth, James C Vickers, David A Mackey, Jamie E Craig, Andrew L Laslett, Alex W Hewitt

2018-09-28

We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex an..

Automated Cell Culture Systems and Their Applications to Human Pluripotent Stem Cell Studies

Maciej Daniszewski, Duncan E Crombie, Rachael Henderson, Helena H Liang, Raymond CB Wong, Alex W Hewitt, Alice Pebay

2018-08-01

Pluripotent stem cells are an extremely powerful tool in modeling human diseases and hold much promise for personalized regenerati..

Longitudinal expression profiling of CD4 and CD8 cells in patients with active to quiescent giant cell arteritis

Elisabeth De Smit, Samuel W Lukowski, Lisa Anderson, Anne Senabouth, Kaisar Dauyey, Sharon Song, Bruce Wyse, Lawrie Wheeler, Christine Y Chen, Khoa Cao, Amy Wong Ten Yuen, Neil Shuey, Linda Clarke, Isabel Lopez Sanchez, Sandy SC Hung, Alice Pebay, David A Mackey, Matthew A Brown, Alex W Hewitt, Joseph E Powell

2018-07-23

BACKGROUND: Giant cell arteritis (GCA) is the most common form of vasculitis affecting elderly people. It is one of the few true o..

Refining retinal ganglion cell (RGC) differentiation from human pluripotent stem cells (hPSCs)

Maciej Daniszewski, Duncan Crombie, Louise Rooney, Alison Conquest, Alex W Hewitt, Alice Pebay

2018-07-01

Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells

Ashfaqul Hogue, Priyadharshini Sivakumaran, Simon T Bond, Naomi XY Ling, Anne M Kong, John W Scott, Nadeeka Bandara, Damian Hernandez, Guei-Sheung Liu, Raymond CB Wong, Michael T Ryan, Derek J Hausenloy, Bruce E Kemp, Jonathan S Oakhill, Brian G Drew, Alice Pebay, Shiang Y Lim

2018-03-05

Human induced pluripotent stem cells (iPSCs) are a valuable tool for studying the cardiac developmental process in vitro, and card..

Single cell RNA sequencing of stem cell-derived retinal ganglion cells

Maciej Brisbane Daniszewski, Anne Senabouth, Quan H Nguyen, Duncan E Crombie, Samuel W Lukowski, Tejal Kulkarni, Valentin M Sluch, Jafar S Jabbari, Xitiz Chamling, Donald J Zack, Alice Pebay, Joseph E Powell, Alex W Hewitt

2018-02-13

We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ga..

Biologically active composition of human W8B2( ) cardiac stem cell secretome

P Sivakumaran, S Nie, X Wang, T Karnezis, N Bandara, C Nowell, S Wilcox, AF Hill, M Shambrook, NC Harris, AE Newcomb, P Strappe, R Shayan, D Hernandez, J Clarke, GJ Dusting, K Takov, S Davidson, E Hanssen, A Pebay

2017-12-01

Drusen in patient-derived hiPSC-RPE models of macular dystrophies

Chad A Galloway, Sonal Dalvi, Sandy SC Hung, Leslie A MacDonald, Lisa R Latchney, Raymond CB Wong, Robyn H Guymer, David A Mackey, David S Williams, Mina M Chung, David M Gamm, Alice Pebay, Alex W Hewitt, Ruchira Singh

2017-09-26

Age-related macular degeneration (AMD) and related macular dystrophies (MDs) are a major cause of vision loss. However, the mechan..

The immortal life of ethics? The alienation of body tissue, ethics and the informed consent procedure within induced pluripotent stem cell research

C MacGregor, T McCaughey, M Munsie, A Pébay, A Hewitt

2017-09-12

Friedreich's ataxia induced pluripotent stem cell-derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency

Duncan E Crombie, Claire L Curl, Antonia JA Raaijmakers, Priyadharshini Sivakumaran, Tejal Kulkarni, Raymond CB Wong, Itsunari Minami, Marguerite V Evans-Galea, Shiang Y Lim, Lea Delbridge, Louise A Corben, Mirella Dottori, Norio Nakatsuji, Ian A Trounce, Alex W Hewitt, Martin B Delatycki, Martin F Pera, Alice Pebay

2017-05-01

We sought to identify the impacts of Friedreich's ataxia (FRDA) on cardiomyocytes. FRDA is an autosomal recessive degenerative con..

Mitochondrial replacement in an iPSC model of Leber's hereditary optic neuropathy

Raymond CB Wong, Shiang Y Lim, Sandy SC Hung, Stacey Jackson, Shahnaz Khan, Nicole J Van Bergen, Elisabeth De Smit, Helena H Liang, Lisa S Kearns, Linda Clarke, David A Mackey, Alex W Hewitt, Ian A Trounce, Alice Pebay

2017-04-01

Cybrid technology was used to replace Leber hereditary optic neuropathy (LHON) causing mitochondrial DNA (mtDNA) mutations from pa..

Lipidomics of Stem Cells

Alice Pébay, Raymond CB Wong

2017-02-24

This book combines the current knowledge on the role of lipids in stem cell pluripotency and differentiation.

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Frisca Frisca, Daniel Colquhoun, Yona Goldshmit, Minna-Liisa Anko, Alice Pebay, Jan Kaslin

2016-11-24

Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and orga..

LONGITUDINAL EXPRESSION PROFILING OF T LYMPHOCYTES IN PATIENTS WITH ACTIVE TO QUIESCENT GIANT CELL ARTERITIS

Elisabeth De Smit, Linda Clarke, Khoa Ngygen, Neil Shuey, Catherine Hill, Lisa Anderson, David Evans, Matt A Brown, Joseph Powell, Alex W Hewitt

2016-11-01

ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy(aEuro)

Dean G Phelan, David J Anderson, Sara E Howden, Raymond CB Wong, Peter F Hickey, Kate Pope, Gabrielle R Wilson, Alice Pebay, Andrew M Davis, Steven Petrou, Andrew G Elefanty, Edouard G Stanley, Paul A James, Ivan Macciocca, Melanie Bahlo, Michael M Cheung, David J Amor, David A Elliott, Paul J Lockhart

2016-09-01

AIMS: We identified a novel homozygous truncating mutation in the gene encoding alpha kinase 3 (ALPK3) in a family presenting with..

Participant understanding and recall of informed consent for induced pluripotent stem cell biobanking

Tristan McCaughey, Christine Y Chen, Elisabeth De Smit, Gwyneth Rees, Eva Fenwick, Lisa S Kearns, David A Mackey, Casimir MacGregor, Megan Munsie, Anthony L Cook, Alice Pebay, Alex W Hewitt

2016-09-01

The ability to generate human induced pluripotent stem cells (iPSCs) has opened new avenues for human disease modelling and therap..

Enriched retinal ganglion cells derived from human embryonic stem cells

Katherine P Gill, Sandy SC Hung, Alexei Sharov, Camden Y Lo, Karina Needham, Grace E Lidgerwood, Stacey Jackson, Duncan E Crombie, Bryony A Nayagam, Anthony L Cook, Alex W Hewitt, Alice Pebay, Raymond CB Wong

2016-08-10

Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cel..

Genome engineering in ophthalmology: Application of CRISPR/Cas to the treatment of eye disease

Sandy SC Hung, Tristan McCaughey, Olivia Swann, Alice Pebay, Alex W Hewitt

2016-07-01

The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and CRISPR-associated protein (Cas) system has enabled an ac..

Targeted therapeutic mild hypercapnia after cardiac arrest: A phase II multi-centre randomised controlled trial (the CCC trial)

Glenn M Eastwood, Antoine G Schneider, Satoshi Suzuki, Leah Peck, Helen Young, Aiko Tanaka, Johan Martensson, Stephen Warrillow, Shay McGuinness, Rachael Parke, Eileen Gilder, Lianne Mccarthy, Pauline Galt, Gopal Taori, Suzanne Eliott, Tammy Lamac, Michael Bailey, Nerina Harley, Deborah Barge, Carol L Hodgson

2016-07-01

BACKGROUND: In intensive care observational studies, hypercapnia after cardiac arrest (CA) is independently associated with improv..

AAV-Mediated CRISPR/Cas Gene Editing of Retinal Cells In Vivo

Sandy SC Hung, Vicki Chrysostomou, Fan Li, Jeremiah KH Lim, Jiang-Hui Wang, Joseph E Powell, Leilei Tu, Maciej Daniszewski, Camden Lo, Raymond C Wong, Jonathan G Crowston, Alice Pebay, Anna E King, Bang V Bui, Guei-Sheung Liu, Alex W Hewitt

2016-06-01

PURPOSE: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has recently been adap..

Using human pluripotent stem cells to study Friedreich ataxia cardiomyopathy

Duncan E Crombie, Martin F Pera, Martin B Delatycki, Alice Pebay

2016-06-01

Friedreich ataxia (FRDA) is the most common of the inherited ataxias. It is an autosomal recessive disease characterised by degene..

A Global Social Media Survey of Attitudes to Human Genome Editing

Tristan McCaughey, Paul G Sanfilippo, George EC Gooden, David M Budden, Li Fan, Eva Fenwick, Gwyneth Rees, Casimir MacGregor, Lei Si, Christine Chen, Helena Hai Liang, Timothy Baldwin, Alice Pebay, Alex W Hewitt

2016-05-05

Ongoing breakthroughs with CRISPR/Cas-based editing could potentially revolutionize modern medicine, but there are many questions ..

Study of mitochondrial respiratory defects on reprogramming to human induced pluripotent stem cells

Sandy SC Hung, Nicole J Van Bergen, Stacey Jackson, Helena Liang, David A Mackey, Damian Hernandez, Shiang Y Lim, Alex W Hewitt, Ian Trounce, Alice Pebay, Raymond CB Wong

2016-05-01

Reprogramming of somatic cells into a pluripotent state is known to be accompanied by extensive restructuring of mitochondria and ..

Defined Medium Conditions for the Induction and Expansion of Human Pluripotent Stem Cell-Derived Retinal Pigment Epithelium

Grace E Lidgerwood, Shiang Y Lim, Duncan E Crombie, Ray Ali, Katherine P Gill, Damian Hernandez, Josh Kie, Alison Conquest, Hayley S Waugh, Raymond CB Wong, Helena H Liang, Alex W Hewitt, Kathryn C Davidson, Alice Pebay

2016-04-01

We demonstrate that a combination of Noggin, Dickkopf-1, Insulin Growth Factor 1 and basic Fibroblast Growth Factor, promotes the ..

An Interactive Multimedia Approach to Improving Informed Consent for Induced Pluripotent Stem Cell Research

Tristan McCaughey, Helena H Liang, Christine Chen, Eva Fenwick, Gwyneth Rees, Raymond CB Wong, James C Vickers, Mathew J Summers, Casimir MacGregor, Jamie E Craig, Megan Munsie, Alice Pebay, Alex W Hewitt

2016-03-03

Cardiac Repair With a Novel Population of Mesenchymal Stem Cells Resident in the Human Heart

Yuan Zhang, Priyadharshini Sivakumaran, Andrew E Newcomb, Damian Hernandez, Nicole Harris, Ramin Khanabdali, Guei-Sheung Liu, Darren J Kelly, Alice Pebay, Alex W Hewitt, Andrew Boyle, Richard Harvey, Wayne A Morrison, David A Elliott, Gregory J Dusting, Shiang Y Lim

2015-10-01

Cardiac resident stem cells (CRSCs) hold much promise to treat heart disease but this remains a controversial field. Here, we desc..

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Sandy SC Hung, Alice Pebay, Raymond CB Wong

2015-08-01

Recent advances in reprogramming allow us to turn somatic cells into human induced pluripotent stem cells (hiPSCs). Disease modeli..

DECREASED ANTI-REGENERATIVE EFFECTS AFTER SPINAL CORD INJURY IN SPRY4-/- MICE

Y Goldshmit, F Frisca, J Kaslin, AR Pinto, J-KKY Tang, A Pebay, R Pinkas-Kramarski, PD Currie

2015-02-26

Previously, we have demonstrated a role for fibroblast growth factor (Fgf) in spinal cord regeneration in both zebrafish and mouse..

Pluripotent stem cell-based models to investigate retinal pigmented epithelium function and disease

DE Crombie, A Pébay, RH Guymer, KC Davidson

2013-02-04

Animal models do not always recapitulate or translate to human diseases. Pluripotent stem cells provide a self-renewing resource t..