Engineering Bioresponsive Hybrid Nanodevices using RNA, Peptides and Synthetic Polymers
Grant number: FT140100873 | Funding period: 2015 - 2019
This project aims to develop new microRNA polymer nanoassemblies with enhanced bioresponsive properties. Nanoengineered functional RNA-polymer materials can exploit both the recognition properties of RNA and the responsiveness of the polymer to tailor the structural and physicochemical properties of such systems. Further, targeting ligands (antibody, aptamers) tethered to the RNA-polymer nanoassembly could augment cellular uptake and delivery. The development of imaging and tracking methodology to accurately measure intracellular miRNA payload and localisation in a 3D tissue mimicking matrix is also planned.
Related publications (26)
The mechanical regulation of RNA binding protein hnRNPC in the failing heart
Fabiana Martino, Nandan Mysore Varadarajan, Ana Rubina Perestrelo, Vaclav Hejret, Helena Durikova, Dragana Vukic, Vladimir Horvath, Francesca Cavalieri, Frank Caruso, Waleed S Albihlal, Andre P Gerber, Mary A O'Connell, Stepanka Vanacova, Stefania Pagliari, Giancarlo Forte
Cardiac pathologies are characterized by intense remodeling of the extracellular matrix (ECM) that eventually leads to heart failu..
Transforming the Chemical Structure and Bio-Nano Activity of Doxorubicin by Ultrasound for Selective Killing of Cancer Cells
Sukhvir Kaur Bhangu, Soraia Fernandes, Giovanni Luca Beretta, Stella Tinelli, Marco Cassani, Agata Radziwon, Marcin Wojnilowicz, Sophia Sarpaki, Irinaios Pilatis, Nadia Zaffaroni, Giancarlo Forte, Frank Caruso, Muthupandian Ashokkumar, Francesca Cavalieri
Reconfiguring the structure and selectivity of existing chemotherapeutics represents an opportunity for developing novel tumor-sel..
Triggering the nanophase separation of albumin through multivalent binding to glycogen for drug delivery in 2D and 3D multicellular constructs
Agata Radziwon, Sukhvir K Bhangu, Soraia Fernandes, Christina Cortez-Jugo, Robert De Rose, Brendan Dyett, Marcin Wojnilowicz, Petra Laznickova, Jan Fric, Giancarlo Forte, Frank Caruso, Francesca Cavalieri
Engineered nanoparticles for the encapsulation of bioactive agents hold promise to improve disease diagnosis, prevention and thera..
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses
Federico Tidu, Marco De Zuani, Shyam Sushama Jose, Kamila Bendickova, Lukas Kubala, Frank Caruso, Francesca Cavalieri, Giancarlo Forte, Jan Fric
Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being teste..
Dissecting the intracellular signalling and fate of a DNA nanosensor by super-resolution and quantitative microscopy.
Agata Glab, Alessandro Bertucci, Fabiana Martino, Marcin Wojnilowicz, Alessia Amodio, Mariano Venanzi, Francesco Ricci, Giancarlo Forte, Frank Caruso, Francesca Cavalieri
DNA nanodevices have been developed as platforms for the manipulation of gene expression, delivery of molecular payloads, and dete..
Nanoengineering multifunctional hybrid interfaces using adhesive glycogen nanoparticles.
Pietro Pacchin Tomanin, Jiajing Zhou, Alessia Amodio, Rita Cimino, Agata Glab, Francesca Cavalieri, Frank Caruso
Multifunctional and biodegradable nanostructured hybrid interfaces based on biopolymers are potentially useful in many application..
Synthesis of bio-functional nanoparticles from sono-responsive amino acids using high frequency ultrasound.
Sukhvir Kaur Bhangu, Muthupandian Ashokkumar, Francesca Cavalieri
A simple, one-pot high frequency ultrasonication (490 kHz) methodology to convert hydrophobic and amphipathic amino acids into nan..
Sound-driven dissipative self-assembly of aromatic biomolecules into functional nanoparticles
Sukhvir Kaur Bhangu, Gianfranco Bocchinfuso, Muthupandian Ashokkumar, Francesca Cavalieri
Dissipative self-assembly processes were recently exploited to assemble synthetic materials into supramolecular structures. In mos..
Modular Assembly of Host-Guest Metal-Phenolic Networks Using Macrocyclic Building Blocks
Shuaijun Pan, Rui Guo, Nadja Bertleff-Zieschang, Shanshan Li, Quinn A Besford, Qi-Zhi Zhong, Gyeongwon Yun, Yunti Zhang, Francesca Cavalieri, Yi Ju, Eirini Goudeli, Joseph J Richardson, Frank Caruso
The manipulation of interfacial properties has broad implications for the development of high‐performance coatings. Metal–phenolic..
Super-resolution Imaging of Proton Sponge-Triggered Rupture of Endosomes and Cytosolic Release of Small Interfering RNA
Marcin Wojnilowicz, Agata Glab, Alessandro Bertucci, Frank Caruso, Francesca Cavalieri
The intracellular delivery of nucleic acids and proteins remains a key challenge in the development of biological therapeutics. In..
Cobalt Phosphate Nanostructures for Non-Enzymatic Glucose Sensing at Physiological pH
Pietro Pacchin Tomanin, Pavel V Cherepanov, Quinn A Besford, Andrew J Christofferson, Alessia Amodio, Chris F McConville, Irene Yarovsky, Frank Caruso, Francesca Cavalieri
Nanostructured materials have potential as platforms for analytical assays and catalytic reactions. Herein, we report the synthesi..
Ultrasound-Assisted Synthesis of Cross-Linked Poly(ethylene glycol) Nanostructures with Hydrophobic Core and Hydrophilic Shell
Haiyan Zhu, Francesca Cavalieri, Muthupandian Ashokkumar
One-pot synthesis of polymeric nanostructures, by using ultrasound without adding initiators and surfactants, is a straightforward..
Self-Assembled Metal-Phenolic Networks on Emulsions as Low-Fouling and pH-Responsive Particles
Quinn A Besford, Yi Ju, Ting-Yi Wang, Gyeongwon Yun, PavelV Cherepanov, Christoph E Hagemeyer, Francesca Cavalieri, Frank Caruso
Interfacial self-assembly is a powerful organizational force for fabricating functional nanomaterials, including nanocarriers, for..
Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids
Marcin Wojnilowicz, Quinn A Besford, Yun-Long Wu, Xian Jun Loh, Julia A Braunger, Agata Glab, Christina Cortez-Jugo, Frank Caruso, Francesca Cavalieri
The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therape..
Sono-transformation of tannic acid into biofunctional ellagic acid micro/nanocrystals with distinct morphologies
Sukhvir Kaur Bhangu, Ritu Singla, Enrico Colombo, Muthupandian Ashokkumar, Francesca Cavalieri
A sustainable, reagent-less and one-pot ultrasonic methodology has been developed to transform amorphous tannic acid into regularl..
Probing Transcription Factor Binding Activity and Downstream Gene Silencing in Living Cells with a DNA Nanoswitch
A Bertucci, J Guo, N Oppmann, A Glab, F Ricci, F Caruso, F Cavalieri
Transcription factor DNA binding activity is of pivotal importance in living systems because of its primary involvement in the reg..
A Simple One-Step Ultrasonic Route To Synthesize Antioxidant Molecules and Fluorescent Nanoparticles from Phenol and Phenol Like Molecules
Sukhvir Kaur Bhangu, Muthupandian Ashokkumar, Francesca Cavalieri
Fluorescent oligomeric structures were synthesized from the phenolic moieties by a simple one-step sonochemical approach without t..
Hyaluronic Acid Nanoporous Microparticles with Long In Vivo Joint Residence Time and Sustained Release
Graziana Palmieri, Antonio Rinaldi, Luisa Campagnolo, Mariarosaria Tortora, Maria Federica Caso, Maurizio Mattei, Andrea Notargiacomo, Nicola Rosato, Massimo Bottini, Francesca Cavalieri
A simple approach is reported to engineer biodegradable and biocompatible nanoporous hyaluronic acid particles (NPHAs) with a char..
Lactosylated Glycogen Nanoparticles for Targeting Prostate Cancer Cells
Quinn A Besford, Marcin Wojnilowicz, Tomoya Suma, Nadja Bertleff-Zieschang, Frank Caruso, Francesca Cayalieri
Glyconanoparticles that exhibit multivalent binding to lectins are desirable for molecular recognition and therapeutic application..
Biofunctional metal-phenolic films from dietary flavonoids
Nadja Bertleff-Zieschang, Md Arifur Rahim, Yi Ju, Julia A Braunger, Tomoya Suma, Yunlu Dai, Shuaijun Pan, Francesca Cavalieri, Frank Caruso
We assembled dietary, bioactive flavonoids into a metal coordinated network to form thin, surface-bound films and hollow capsules,..
Sono-assembly of nanostructures via tyrosine-tyrosine coupling reactions at the interface of acoustic cavitation bubbles
Francesca Cavalieri, Enrico Colombo, Eleonora Nicolai, Nicola Rosato, Muthupandian Ashokkumar
This work demonstrates that the reactive and oscillating surface of acoustic cavitation microbubbles acts as a catalytic binding s..
A combined approach for predicting the cytotoxic effect of drug-nanoaggregates
M Wojnilowicz, M Tortora, BG Bobay, E Santiso, M Caruso, L Micheli, M Venanzi, S Menegatti, F Cavalieri
We present a combined spectroscopic and computational approach aimed to elucidate the mechanism of formation and activity of etopo..
Probing cell internalisation mechanics with polymer capsules
Xi Chen, Jiwei Cui, Yuan Ping, Tomoya Suma, Francesca Cavalieri, Quinn A Besford, George Chen, Julia A Braunger, Frank Caruso
We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(me..
Redox-Sensitive PEG-Polypeptide Nanoporous Particles for Survivin Silencing in Prostate Cancer Cells
Francesca Cavalieri, Giovanni L Beretta, Jiwei Cui, Julia A Braunger, Yan Yan, Joseph J Richardson, Stella Tinelli, Marco Folini, Nadia Zaffaroni, Frank Caruso
We report the engineering of intracellular redox-responsive nanoporous poly(ethylene glycol)-poly(l-lysine) particles (NPEG-PLLs)...