GENOMIC APPROACHES IN HUMAN HEALTH MICROBIOLOGY
Grant number: 1105525 | Funding period: 2016 - 2020
This project uses latest genomic technologies to advance our understanding of how bacteria cause disease and finding new antibiotics/approaches to stop the spread of multi-drug resistant hospital superbugs.
Related publications (20)
Population genomics provides insights into the evolution and adaptation to humans of the waterborne pathogen Mycobacterium kansasii
Tao Luo, Peng Xu, Yangyi Zhang, Jessica L Porter, Marwan Ghanem, Qingyun Liu, Yuan Jiang, Jing Li, Qing Miao, Bijie Hu, Benjamin P Howden, Janet AM Fyfe, Maria Globan, Wencong He, Ping He, Yiting Wang, Houming Liu, Howard E Takiff, Yanlin Zhao, Xinchun Chen
Mycobacterium kansasii can cause serious pulmonary disease. It belongs to a group of closely-related species of non-tuberculous my..
Tracking the COVID-19 pandemic in Australia using genomics
Torsten Seemann, Courtney R Lane, Norelle L Sherry, Sebastian Duchene, Anders Goncalves da Silva, Leon Caly, Michelle Sait, Susan A Ballard, Kristy Horan, Mark B Schultz, Tuyet Hoang, Marion Easton, Sally Dougall, Timothy P Stinear, Julian Druce, Mike Catton, Brett Sutton, Annaliese van Diemen, Charles Alpren, Deborah A Williamson
Genomic sequencing has significant potential to inform public health management for SARS-CoV-2. Here we report high-throughput gen..
High antibody titres induced by protein subunit vaccines using Mycobacterium ulcerans antigens Hsp18 and MUL _3720 with a TLR-2 agonist fail to protect against Buruli ulcer in mice
Kirstie M Mangas, Nicholas J Tobias, Estelle Marion, Jeremie Babonneau, Laurent Marsollier, Jessica L Porter, Sacha J Pidot, Chinn Yi Wong, David C Jackson, Brendon Y Chua, Timothy P Stinear
Background: Mycobacterium ulcerans is the causative agent of a debilitating skin and soft tissue infection known as Buruli ulcer (..
Microbe-Metabolite Associations Linked to the Rebounding Murine Gut Microbiome Postcolonization with Vancomycin-Resistant Enterococcus faecium
Andre Mu, Glen P Carter, Lucy Li, Nicole S Isles, Alison F Vrbanac, James T Morton, Alan K Jarmusch, David P De Souza, Vinod K Narayana, Komal Kanojia, Brunda Nijagal, Malcolm J McConville, Rob Knight, Benjamin P Howden, Timothy P Stinear
Vancomycin-resistant Enterococcus faecium (VREfm) is an emerging antibiotic-resistant pathogen. Strain-level investigations are be..
Comparative Transcriptomic and Functional Assessments o Linezolid-Responsive Small RNA Genes in Staphylococcus aureus
Wei Gao, Romain Guerillot, Ya Hsun Lin, Jai Tree, Marie Beaume, Patrice Francois, Ian R Monk, Torsten Seemann, Jacques Schrenzel, Benjamin P Howden, Timothy P Stinear
Staphylococcus aureus contains a repertoire of at least 50 and possibly 500 small RNAs (sRNAs). The functions of most sRNAs are no..
Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA
Jean YH Lee, Nickala Best, Julie McAuley, Jessica L Porter, Torsten Seemann, Mark B Schultz, Michelle Sait, Nicole Orlando, Karolina Mercoulia, Susan A Ballard, Julian Druce, Thomas Tran, Mike G Catton, Melinda J Pryor, Huanhuan L Cui, Angela Luttick, Sean McDonald, Arran Greenhalgh, Jason C Kwong, Norelle L Sherry
Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes requir..
Mining the Methylome Reveals Extensive Diversity in Staphylococcus epidermidis Restriction Modification
Jean YH Lee, Glen P Carter, Sacha J Pidot, Romain Guerillot, Torsten Seemann, Anders Goncalves da Silva, Timothy J Foster, Benjamin P Howden, Timothy P Stinear, Ian R Monk
Staphylococcus epidermidis is a significant opportunistic pathogen of humans. Molecular studies in this species have been hampered..
Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase of Staphylococcus aureus
Ian R Monk, Nausad Shaikh, Stephanie L Begg, Mike Gajdiss, Liam KR Sharkey, Jean YH Lee, Sacha J Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett M Collins, Gabriele Bierbaum, Saumya R Udagedara, Jacqueline R Morey, Neha Pulyani, Benjamin P Howden, Megan J Maher, Christopher A McDevitt, Glenn F King
WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidogl..
Global spread of three multidrug-resistant lineages of Staphylococcus epidermidis
Jean YH Lee, Ian R Monk, Anders Goncalves da Silva, Torsten Seemann, Kyra YL Chua, Angela Kearns, Robert Hill, Neil Woodford, Mette D Bartels, Birgit Strommenger, Frederic Laurent, Magali Dodemont, Ariane Deplano, Robin Patel, Anders R Larsen, Tony M Korman, Timothy P Stinear, Benjamin P Howden
Staphylococcus epidermidis is a conspicuous member of the human microbiome, widely present on healthy skin. Here we show that S. e..
Zinc-binding to the cytoplasmic PAS domain regulates the essential WalK histidine kinase ofStaphylococcus aureus
Ian Monk, Nausad Shaikh, Stephanie Begg, Mike Gajdiss, Jean Lee, Sacha Pidot, Torsten Seemann, Michael Kuiper, Brit Winnen, Rikki Hvorup, Brett Collins, Gabriele Bierbaum, Benjamin Howden, Christopher McDevitt, Glenn King, Timothy Stinear
<h4>ABSTRACT</h4> WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR r..
Increasing tolerance of hospital Enterococcus faecium to handwash alcohols
Sacha J Pidot, Wei Gao, Andrew H Buultjens, Ian R Monk, Romain Guerillot, Glen P Carter, Jean YH Lee, Margaret MC Lam, M Lindsay Grayson, Susan A Ballard, Andrew A Mahony, Elizabeth A Grabsch, Despina Kotsanas, Tony M Korman, Geoffrey W Coombs, J Owen Robinson, Anders Goncalves da Silva, Torsten Seemann, Benjamin P Howden, Paul DR Johnson
Alcohol-based disinfectants and particularly hand rubs are a key way to control hospital infections worldwide. Such disinfectants ..
Human blood MAIT cell subsets defined using MR1 tetramers
Nicholas A Gherardin, Michael NT Souter, Hui-Fern Koay, Kirstie M Mangas, Torsten Seemann, Timothy P Stinear, Sidonia BG Eckle, Stuart P Berzins, Yves d'Udekem, Igor E Konstantinov, David P Fairlie, David S Ritchie, Paul J Neeson, Daniel G Pellicci, Adam P Uldrich, James McCluskey, Dale I Godfrey
Mucosal-associated invariant T (MAIT) cells represent up to 10% of circulating human T cells. They are usually defined using combi..
Comparative Genomics Shows That Mycobacterium ulcerans Migration and Expansion Preceded the Rise of Buruli Ulcer in Southeastern Australia
Andrew H Buultjens, Koen Vandelannoote, Conor J Meehan, Miriam Eddyani, Bouke C de Jong, Janet AM Fyfe, Maria Globan, Nicholas J Tobias, Jessica L Porter, Takehiro Tomita, Ee Laine Tay, Torsten Seemann, Benjamin P Howden, Paul DR Johnson, Timothy P Stinear
Since 2000, cases of the neglected tropical disease Buruli ulcer, caused by infection with Mycobacterium ulcerans, have increased ..
Translating genomics into practice for real-time surveillance and response to carbapenemase-producing Enterobacteriaceae: evidence from a complex multi-institutional KPC outbreak
Jason C Kwong, Courtney R Lane, Finn Romanes, Anders Goncalves da Silva, Marion Easton, Katie Cronin, Mary Jo Waters, Takehiro Tomita, Kerrie Stevens, Mark B Schultz, Sarah L Baines, Norelle L Sherry, Glen P Carter, Andre Mu, Michelle Sait, Susan A Ballard, Torsten Seemann, Timothy P Stinear, Benjamin P Howden
Background: Until recently, Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were rarely identified in Austr..
Real-time genomic and epidemiological investigation of a multi-institution outbreak of KPC-producing Enterobacteriaceae: a translational study
JC Kwong, C Lane, F Romanes, A Gonçalves da Silva, M Easton, K Cronin, MJ Waters, T Tomita, K Stevens, MB Schultz, SL Baines, NL Sherry, G Carter, A Mu, M Sait, SA Ballard, T Seemann, TP Stinear, BP Howden
<h4>ABSTRACT</h4> <h4>Background</h4> Until recently, KPC-producing Enterobacteriaceae were rarely identified in Australia. Follow..
Bacterial membrane vesicles transport their DNA cargo into host cells
Natalie J Bitto, Ross Chapman, Sacha Pidot, Adam Costin, Camden Lo, Jasmine Choi, Tanya D'Cruze, Eric C Reynolds, Stuart G Dashper, Lynne Turnbull, Cynthia B Whitchurch, Timothy P Stinear, Katryn J Stacey, Richard L Ferrero
Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wa..
Target-Specific Assay for Rapid and Quantitative Detection of Mycobacterium chimaera DNA
Enrique Zozaya-Valdes, Jessica L Porter, John Coventry, Janet AM Fyfe, Glen P Carter, Anders Goncalves da Silva, Mark B Schultz, Torsten Seemann, Paul DR Johnson, Andrew J Stewardson, Ivan Bastian, Sally A Roberts, Benjamin P Howden, Deborah A Williamson, Timothy P Stinear
Mycobacterium chimaera is an opportunistic environmental mycobacterium belonging to the Mycobacterium avium-M. intracellulare comp..
Multiple Introductions and Recent Spread of the Emerging Human Pathogen Mycobacterium ulcerans across Africa
Koen Vandelannoote, Conor J Meehan, Miriam Eddyani, Dissou Affolabi, Delphin Mavinga Phanzu, Sara Eyangoh, Kurt Jordaens, Francoise Portaels, Kirstie Mangas, Torsten Seemann, Laurent Marsollier, Estelle Marion, Annick Chauty, Jordi Landier, Arnaud Fontanet, Herwig Leirs, Timothy P Stinear, Bouke C de Jong
Buruli ulcer (BU) is an insidious neglected tropical disease. Cases are reported around the world but the rural regions of West an..