NEW ANTBIOTICS FROM OLD MICROBES

Natural product discovery through microbial genome mining.

Emma Kenshole, Marion Herisse, Michael Michael, Sacha J Pidot

2021-02-01

The advent of the genomic era has opened up enormous possibilities for the discovery of new natural products. Also known as specia..

Oak-Associated Negativicute Equipped with Ancestral Aromatic Polyketide Synthase Produces Antimycobacterial Dendrubins

Keishi Ishida, Gulimila Shabuer, Sebastian Schieferdecker, Sacha J Pidot, Timothy P Stinear, Uwe Knuepfer, Michael Cyrulies, Christian Hertweck

2020-09-17

Anaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (G..

Identification and Mobilization of a Cryptic Antibiotic Biosynthesis Gene Locus from a Human-Pathogenic Nocardia Isolate

Marion Herisse, Keishi Ishida, Jessica L Porter, Ben Howden, Christian Hertweck, Timothy P Stinear, Sacha J Pidot

2020-05-15

The genus Nocardia contains >50 human pathogenic species that cause a range of illnesses from skin and soft tissue infections to l..

Mining Symbionts of a Spider-Transmitted Fungus Illuminates Uncharted Biosynthetic Pathways to Cytotoxic Benzolactones

Sarah P Niehs, Benjamin Dose, Sophie Richter, Sacha J Pidot, Hans-Martin Dahse, Timothy P Stinear, Christian Hertweck

2020-03-18

A spider-transmitted fungus (Rhizopus microsporus) that was isolated from necrotic human tissue was found to harbor endofungal bac..

The Nargenicin Family of Oxa-Bridged Macrolide Antibiotics

Sacha J Pidot, Mark A Rizzacasa

2019-12-16

The nargenicin family of antibiotic macrolides comprise a group of bacterial natural products with a rare ether bridged cis-decali..

Genomics‐Driven Discovery of NO‐Donating Diazeniumdiolate Siderophores in Diverse Plant‐Associated Bacteria

Ron Hermenau, Jule L Mehl, Keishi Ishida, Benjamin Dose, Sacha J Pidot, Timothy P Stinear, Christian Hertweck

2019-09-09

Siderophores are key players in bacteria–host interactions, with the main function to provide soluble iron for their producers. Gr..

Genomics-Driven Discovery of NO-Donating Diazeniumdiolate Siderophores in Diverse Plant-Associated Bacteria

Ron Hermenau, Jule L Mehl, Keishi Ishida, Benjamin Dose, Sacha Pidot, Timothy P Stinear, Christian Hertweck

2019-08-05

Siderophores are key players in bacteria-host interactions, with the main function to provide soluble iron for their producers. Gr..

Genome Mining Reveals Endopyrroles from a Nonribosomal Peptide Assembly Line Triggered in Fungal-Bacterial Symbiosis

Sarah P Niehs, Benjamin Dose, Kirstin Scherlach, Sacha J Pidot, Timothy P Stinear, Christian Hertweck

2019-08-01

The bacterial endosymbiont (Burkholderia rhizoxinica) of the rice seedling blight fungus (Rhizopus microsporus) harbors a large nu..

Biosynthesis of Diverse Antimicrobial and Antiproliferative Acyloins in Anaerobic Bacteria

Sebastian Schieferdecker, Gulimila Shabuer, Anne-Catrin Letzel, Barbara Urbansky, Mie Ishida-Ito, Keishi Ishida, Michael Cyrulies, Hans-Martin Dahse, Sacha Pidot, Christian Hertweck

2019-07-01

Metabolic profiling and genome mining revealed that anaerobic bacteria have the potential to produce acyloin natural products. In ..

Biosynthesis and Ether-Bridge Formation in Nargenicin Macrolides

Sacha J Pidot, Marion Herisse, Liam Sharkey, Liselle Atkin, Jessica L Porter, Torsten Seemann, Benjamin P Howden, Mark A Rizzacasa, Timothy P Stinear

2019-03-18

The nargenicin family of antibiotics are macrolides containing a rare ether-bridged cis-decalin motif. Several of these compounds ..

Biosynthesis and Ether‐Bridge Formation in Nargenicin Macrolides

Sacha J Pidot, Marion Herisse, Liam Sharkey, Liselle Atkin, Jessica L Porter, Torsten Seemann, Benjamin P Howden, Mark A Rizzacasa, Timothy P Stinear

2019-03-18

Abstract: The nargenicin family of antibiotics are macrolides containing a rare ether‐bridged cis‐decalin motif. Several of these ..

The Phi BT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia

Marion Herisse, Jessica L Porter, Romain Guerillot, Takehiro Tomita, Anders Goncalves Da Silva, Torsten Seemann, Benjamin P Howden, Timothy P Stinear, Sacha J Pidot

2018-05-04

Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DN..

Natural product diversity associated with the nematode symbionts Photorhabdus and Xenorhabdus

Nicholas J Tobias, Hendrik Wolff, Bardya Djahanschiri, Florian Grundmann, Max Kronenwerth, Yi-Ming Shi, Svenja Simonyi, Peter Gruen, David Shapiro-Ilan, Sacha J Pidot, Timothy P Stinear, Ingo Ebersberger, Helge B Bode

2017-12-01

Xenorhabdus and Photorhabdus species dedicate a large amount of resources to the production of specialized metabolites derived fro..

Structure and Biosynthesis of Isatropolones, Bioactive Amine-Scavenging Fluorescent Natural Products from Streptomyces Go66

Xiaofeng Cai, Yi-Ming Shi, Nicole Poehlmann, Ole Revermann, Isabel Bahner, Sacha J Pidot, Frank Wesche, Helmut Lackner, Claudia Buechel, Marcel Kaiser, Christian Richter, Harald Schwalbe, Timothy P Stinear, Axel Zeeck, Helge B Bode

2017-04-24

The natural products isatropolone A-C (1-3) were reisolated from Streptomyces Gö66, with 1 and 3 showing potent activity against L..

Struktur und Biosynthese der Isatropolone, bioaktiver und Amin-reaktiver fluoreszierender Naturstoffe aus Streptomyces Gö66

Xiaofeng Cai, Yi-Ming Shi, Nicole Pöhlmann, Ole Revermann, Isabel Bahner, Sacha J Pidot, Frank Wesche, Helmut Lackner, Claudia Büchel, Marcel Kaiser, Christian Richter, Harald Schwalbe, Timothy P Stinear, Axel Zeeck, Helge B Bode

2017-04-24