STANDARDISING PROTOCOLS FOR THE DIFFERENTIATION AND INTEGRATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED NEURAL TRANSPLANTS IN PARKINSON'S DISEASE
Grant number: 1102704 | Funding period: 2016 - 2019
Clinical trials have shown that transplanting dopamine neurons (specific nerve cells) into the brain of Parkinson’s disease patients can improve symptoms. Trials use fetal tissue for implantation, which is unsustainable and highly variable. This proposal will examine stem cells as an alternative. We will establish a reliable protocol to instruct human stem cells to become dopamine neurons, develop methods to select these cells and, examine the integration of these transplanted cells in the brain
Related publications (6)
An Optimized Protocol for the Generation of Midbrain Dopamine Neurons under Defined Conditions.
Carlos W Gantner, Agustín Cota-Coronado, Lachlan H Thompson, Clare L Parish
Here, we describe a xeno-free, feeder-free, and chemically defined protocol for the generation of ventral midbrain dopaminergic (v..
Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
Carlos W Gantner, Isabelle R de Luzy, Jessica A Kauhausen, Niamh Moriarty, Jonathan C Niclis, Christopher R Bye, Vanessa Penna, Cameron PJ Hunt, Charlotte M Ermine, Colin W Pouton, Deniz Kirik, Lachlan H Thompson, Clare L Parish
Dopaminergic neurons (DAns), generated from human pluripotent stem cells (hPSCs), are capable of functionally integrating followin..
Isolation of LMX1a Ventral Midbrain Progenitors Improves the Safety and Predictability of Human Pluripotent Stem Cell-Derived Neural Transplants in Parkinsonian Disease
Isabelle R de Luzy, Jonathan C Niclis, Carlos W Gantner, Jessica A Kauhausen, Cameron PJ Hunt, Charlotte Ermine, Colin W Pouton, Lachlan H Thompson, Clare L Parish
Human pluripotent stem cells (hPSCs) are a promising resource for the replacement of degenerated ventral midbrain dopaminergic (vm..
Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain
Christopher R Bye, Vanessa Penna, Isabelle R de Luzy, Carlos W Gantner, Cameron PJ Hunt, Lachlan H Thompson, Clare L Parish
Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited..
Local Injection of Endothelin-1 in the Early Neonatal Rat Brain Models Ischemic Damage Associated with Motor Impairment and Diffuse Loss in Brain Volume
Jordan L Wright, Hannah X Chu, Brett J Kagan, Charlotte M Ermine, Jessica A Kauhausen, Clare L Parish, Christopher G Sobey, Lachlan H Thompson
Cerebral palsy is an irreversible movement disorder resulting from cerebral damage sustained during prenatal or neonatal brain dev..
A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells
Jonathan C Niclis, Carlos W Gantner, Cameron PJ Hunt, Jessica A Kauhausen, Jennifer C Durnall, John M Haynes, Colin W Pouton, Clare L Parish, Lachlan H Thompson
Development of safe and effective stem cell-based therapies for brain repair requires an in-depth understanding of the in vivo pro..