Characterisation of membrane protein ubiquitination by MARCH ligases

Abstract

The goal of the project is to understand how a family of enzymes called MARCHs regulate expression and localisation of immunoregulatory receptors within cells by post-translational addition of a small protein tag called Ubiquitin. The aims are to decipher the ubiquitination patterns produced by the MARCHs; identify the E2 ligases used by the MARCHs to produce distinct Ub codes; and apply a new proteomic pipeline to identify novel representative MARCH substrates in mice deficient in six different MARCHs. It is anticipated the project will reveal novel insights into a fundamental cell biological process of major significance for regulation of protein expression and trafficking in cells of the ..

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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8

Patrick Schriek, Haiyin Liu, Alan C Ching, Pauline Huang, Nishma Gupta, Kayla R Wilson, MinHsuang Tsai, Yuting Yan, Christophe F Macri, Laura F Dagley, Giuseppe Infusini, Andrew I Webb, Hamish McWilliam, Satoshi Ishido, Justine D Mintern, Jose A Villadangos

<jats:title>ABSTRACT</jats:title><jats:p>MARCH1 and MARCH8 are ubiquitin ligases that control the expression and trafficking of cr..