Atomic details of antimicrobial peptides at work in live cells
Grant number: DP160100959 | Funding period: 2016 - 2019
This project aims to develop methods to determine the detailed structure of biologically important molecules in live cells to better understand how biomolecular structure is related to disease. The structure at the atomic level of the molecules of life is usually characterised by crystal or solution studies in model systems. However, the structure of many biologically important molecules depends on their environment. Using new instrumentation and labelling schemes, the project plans to use nuclear magnetic resonance methods to study antibiotics and antimicrobial peptides in live bacteria and human cells. The goal is to resolve how these molecules cross cell membranes and how biomolecular str..View full description
Related publications (15)
TOAC spin-labeled peptides tailored for DNP-NMR studies in lipid membrane environments.
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The benefit of combining in-cell solid-state dynamic nuclear polarization (DNP) NMR and cryogenic temperatures is providing suffic..
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Shiying Zhu, Wenyi Li, Neil O'Brien-Simpson, Frances Separovic, Marc-Antoine Sani
Cationic antimicrobial peptides have been investigated for their potential use in combating infections by targeting the cell membr..
The antimicrobial peptide maculatin self assembles in parallel to form a pore in phospholipid bilayers
Marc-Antoine Sani, Anton P Le Brun, Frances Separovic
Little is known experimentally about the detailed orientation of membrane-bound maculatin 1.1 (Mac1), an antimicrobial peptide fro..
Phote-HrTH (Phormia terraenovae Hypertrehalosaemic Hormone), the Metabolic Hormone of the Fruit Fly: Solution Structure and Receptor Binding Model
Ibrahim A Abdulganiyyu, Marc-Antoine Sani, Frances Separovic, Heather Marco, Graham E Jackson
Fruit flies are a widely distributed pest insect that pose a significant threat to food security. Flight is essential for the disp..
Cholesterol-Dependent Cytolysins: Membrane and Protein Structural Requirements for Pore Formation
Craig J Morton, Marc-Antoine Sani, Michael W Parker, Frances Separovic
The cholesterol-dependent cytolysins (CDCs) are a family of bacterial protein toxins specifically targeting eukaryotic cells throu..
In Situ Monitoring of Bacteria under Antimicrobial Stress Using P-31 Solid-State NMR
Sarah A Overall, Shiying Zhu, Eric Hanssen, Frances Separovic, Marc-Antoine Sani
In-cell NMR offers great insight into the characterization of the effect of toxins and antimicrobial peptides on intact cells. How..
Implications of peptide assemblies in amyloid diseases
Pu Chun Ke, Marc-Antonie Sani, Feng Ding, Aleksandr Kakinen, Ibrahim Javed, Frances Separovic, Thomas P Davis, Raffaele Mezzenga
Neurodegenerative disorders and type 2 diabetes are global epidemics compromising the quality of life of millions worldwide, with ..
One pathogen two stones: are Australian tree frog antimicrobial peptides synergistic against human pathogens?
Marc-Antoine Sani, Siobhan Carne, Sarah A Overall, Alexandre Poulhazan, Frances Separovic
Antimicrobial peptides (AMPs) may act by targeting the lipid membranes and disrupting the bilayer structure. In this study, three ..