AN ULTRA HIGH RESOLUTION MASS SPECTROMETRY FACILITY FOR LIPIDOMICS RESEARCH
Grant number: LE160100015 | Funding period: 2016 - 2016
This proposal aims to establish an ultra-high-resolution, accurate mass spectrometry facility in Australia for comprehensive lipidomics research. The platform would consist of a Thermo Scientific Orbitrap Fusion mass spectrometer interfaced with ultra-high-pressure high-performance liquid chromatography. This proposal will address a major need for advanced mass spectrometry-based lipid analysis capabilities across mammalian, plant, parasite, and microalgae research disciplines, as well as enabling fundamental studies of lipid separation, chemistry and reactivity. The instrumentation would be applicable to a diverse range of projects including studies of the role of lipid metabolism in mammal..View full description
Related publications (11)
Characterization of brain-derived extracellular vesicle lipids in Alzheimer's disease
Huaqi Su, Yepy H Rustam, Colin L Masters, Enes Makalic, Catriona A McLean, Andrew F Hill, Kevin J Barnham, Gavin E Reid, Laura J Vella
Lipid dyshomeostasis is associated with the most common form of dementia, Alzheimer's disease (AD). Substantial progress has been ..
Effect of Expression of Human Glucosylceramidase 2 Isoforms on Lipid Profiles in COS-7 Cells
Peeranat Jatooratthawichot, Chutima Talabnin, Lukana Ngiwsara, Yepy Hardi Rustam, Jisnuson Svasti, Gavin E Reid, James R Ketudat Cairns
Glucosylceramide (GlcCer) is a major membrane lipid and the precursor of gangliosides. GlcCer is mainly degraded by two enzymes, l..
BAK core dimers bind lipids and can be bridged by them
Angus D Cowan, Nicholas A Smith, Jarrod J Sandow, Eugene A Kapp, Yepy H Rustam, James M Murphy, Jason M Brouwer, Jonathan P Bernardini, Michael J Roy, Ahmad Z Wardak, Iris K Tan, Andrew I Webb, Jacqueline M Gulbis, Brian J Smith, Gavin E Reid, Grant Dewson, Peter M Colman, Peter E Czabotar
BAK and BAX are essential mediators of apoptosis that oligomerize in response to death cues, thereby causing permeabilization of t..
Using electrospray ionization-tandem mass spectrometry to explore formation and gas-phase chemistry of silver nanoclusters generated from the reaction of silver salts with NaBH(4)in the presence of bis(diphenylarsino)methane
Howard Z Ma, Alasdair I McKay, Allan J Canty, Richard AJ O'Hair
Electrospray ionization-mass spectrometry (ESI-MS) of mixtures of AgBF4 or AgNO3 with the capping ligand bis(diphenylarsino)methan..
Structural characterization and gas-phase studies of the [Ag10H8(L)(6)](2 ) nanocluster dication
Howard Z Ma, Alasdair McKay, Antonia Mravak, Michael S Scholz, Jonathan M White, Roger J Mulder, Evan J Bieske, Vlasta Bonacic-Koutecky, Richard AJ O'Hair
The reactions between silver salts and borohydrides produce a rich set of products that range from discrete mononuclear compounds ..
Establishing Signature Fragments for Identification and Sequencing of Dityrosine Cross-Linked Peptides Using Ultraviolet Photodissociation Mass Spectrometry
Soumya Mukherjee, Mengxuan Fang, W Mei Kok, Eugene A Kapp, Varsha J Thombare, Romain Huguet, Craig A Hutton, Gavin E Reid, Blaine R Roberts
Dityrosine cross-linking of Aβ peptides and α-synuclein is increasingly becoming recognized as a biomarker of neuropathological di..
Developmental lipidome and proteome of Haemonchus contortus reflect unique adaptations in the transition to parasitism
S Nie, Tao Wang, Guangxu Ma, Ching-Seng Ang, Pasi Korhonen, Rong Xu, Anson Koehler, Richard SIMPSON, David GREENING, Gavin Reid, Nicholas Williamson, Robin Gasser
There has been a dearth of lipidomic and proteomic data sets to underpin fundamental investigations of parasites and parasitism at..
Synthesis, structure, and condensed-phase reactivity of [Ag-3(mu(3)-H)(mu(3)-BH4)L-3(Ph)](BF4) (L-Ph = bis(diphenylphosphino) amine) with CS2
Howard Z Ma, Jonathan M White, Roger J Mulder, Gavin E Reid, Allan J Canty, Richard AJ O'Hair
Electrospray ionisation mass spectrometry (ESI-MS) was used to monitor the reaction of AgBF4, bis(diphenylphosphino)amine (dppa = ..
Molecular alterations during larval development of Haemonchus contortus in vitro are under tight post-transcriptional control
Guangxu Ma, Tao Wang, Pasi K Korhonen, Ching-Seng Ang, Nicholas A Williamson, Neil D Young, Andreas J Stroehlein, Ross S Hall, Anson V Koehler, Andreas Hofmann, Robin B Gasser
In this study, we explored the molecular alterations in the developmental switch from the L3 to the exsheathed L3 (xL3) and to the..
Synthesis and X-Ray Crystallographic Characterisation of Frustum-Shaped Ligated [Cu18H16(DPPE)(6)](2 ) and [Cu16H14(DPPA)(6)](2 ) Nanoclusters and Studies on Their H-2 Evolution Reactions
Jiaye Li, Howard Z Ma, Gavin E Reid, Alison J Edwards, Yuning Hong, Jonathan M White, Roger J Mulder, Richard AJ O'Hair
We report new structural motifs for Cu nanoclusters that conceptually represent seed crystals for large face-centred cubic (FCC) c..
Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development
Molly Parkyn Schneider, Boyin Liu, Philipp Glock, Annika Suttie, Emma McHugh, Dean Andrew, Steven Batinovic, Nicholas Williamson, Eric Hanssen, Paul McMillan, Marion Hliscs, Leann Tilley, Matthew WA Dixon
Transmission of malaria parasites relies on the formation of a specialized blood form called the gametocyte. Gametocytes of the hu..