Dynamic Nuclear Polarisation System for Molecular Structure Determination

NMR techniques for investigating antimicrobial peptides in model membranes and bacterial cells

Marc-Antoine Sani, Sunnia Rajput, David W Keizer, Frances Separovic

2024-04-01

AMPs are short, mainly cationic membrane-active peptides found in all living organism. They perform diverse roles including signal..

The membrane activity of the antimicrobial peptide caerin 1.1 is pH dependent

MA Sani, AP Le Brun, S Rajput, T Attard, F Separovic

2023-03-21

Antimicrobial peptides are an important class of membrane-active peptides that can provide alternatives or complements to classic ..

Transient RNA Interactions Leave a Covalent Imprint on a Viral Capsid Protein

ZH Taji, P Bielytskyi, M Shein, MA Sani, S Seitz, AK Schütz

2022-05-18

The hepatitis B virus (HBV) is the leading cause of persistent liver infections. Its DNA-based genome is synthesized through rever..

In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide

F Separovic, V Hofferek, AP Duff, MJ McConville, MA Sani

2022-01-01

Dynamic nuclear polarization NMR spectroscopy was used to investigate the effect of the antimicrobial peptide (AMP) maculatin 1.1 ..

TOAC spin-labeled peptides tailored for DNP-NMR studies in lipid membrane environments

S Zhu, E Kachooei, JR Harmer, LJ Brown, F Separovic, MA Sani

2021-10-19

The benefit of combining in-cell solid-state dynamic nuclear polarization (DNP) NMR and cryogenic temperatures is providing suffic..

Expression and purification of the native C-amidated antimicrobial peptide maculatin 1.1

S Zhu, DK Weber, F Separovic, MA Sani

2021-08-01

Maculatin 1.1 (Mac1) is an antimicrobial peptide (AMP) from an Australian tree frog and exhibits low micromolar activity against G..

C-terminus amidation influences biological activity and membrane interaction of maculatin 1.1

S Zhu, W Li, N O’Brien-Simpson, F Separovic, MA Sani

2021-05-01

Cationic antimicrobial peptides have been investigated for their potential use in combating infections by targeting the cell membr..

In-Cell Solid-State NMR Analysis of Membrane Proteins

S Zhu, MA Sani, F Separovic

2021-05-01

Solid-state NMR spectroscopy (ssNMR) has great potential to provide atomistic detail of membrane proteins (MPs) as it is suitable ..

NMR Chemical Shift and Methylation of 4-Nitroimidazole: Experiment and Theory

Frederick Backler, Marc Antoine Sani, Frances Separovic, Vladislav Vasilyev, Feng Wang

2020-10-01

Nitroimidazoles and derivatives are a class of active pharmaceutical ingredients (APIs) first introduced sixty years ago. As anti-..

Nuclear Magnetic Resonance Study of the Peptide FRANCESSEPAROVIC

JA Karas, DW Keizer, MA Sani

2020-03-01

As an eminent ambassador of STEM and renowned NMR spectroscopist, Frances Separovic is an internationally famous name, but could i..

In-cell Solid-State NMR Studies of Antimicrobial Peptides

Frances Separovic, David W Keizer, Marc-Antoine Sani

2020-01-01

Antimicrobial peptides (AMPs) have attracted attention as alternatives to classic antibiotics due to their expected limited pressu..

Nitroxide spin–labeled peptides for DNP-NMR in-cell studies

MA Sani, S Zhu, V Hofferek, F Separovic

2019-10-01

Antimicrobial peptides (AMPs) that target lipid membranes show promise as alternatives to conventional antibiotics. However, the m..

DNP NMR spectroscopy reveals new structures, residues and interactions in wild spider silks

HC Craig, SJ Blamires, MA Sani, MM Kasumovic, A Rawal, JM Hook

2019-01-01

DNP solid state NMR spectroscopy allows non-targeted analysis of wild spider silk in unprecedented detail at natural abundance, re..

Elucidating the bactericidal mechanism of action of the linear antimicrobial tetrapeptide BRBR-NH 2

QY Lau, J Li, MA Sani, S Sinha, Y Li, FM Ng, CB Kang, S Bhattacharjya, F Separovic, C Verma, CSB Chia

2018-08-01

Linear antimicrobial peptides, with their rapid bactericidal mode of action, are well-suited for development as topical antibacter..

Probing Ionic Liquid Electrolyte Structure via the Glassy State by Dynamic Nuclear Polarization NMR Spectroscopy

MA Sani, PA Martin, R Yunis, F Chen, M Forsyth, M Deschamps, LA O'Dell

2018-03-01

Dynamic nuclear polarization (DNP)-enhanced solid-state NMR spectroscopy has been used to study an ionic liquid salt solution (N-m..