Unraveling the cell wall substrate delivery mechanisms in plants

Cell surface carbohydrates of symbiotic dinoflagellates and their role in the establishment of cnidarian-dinoflagellate symbiosis

Giada Tortorelli, Carsten Rautengarten, Antony Bacic, Gabriela Segal, Berit Ebert, Simon K Davy, Madeleine JH van Oppen, Geoffrey McFadden

2021-07-20

Symbiodiniaceae algae are often photosymbionts of reef-building corals. The establishment of their symbiosis resembles a microbial..

Not Just a Simple Sugar: Arabinose Metabolism and Function in Plants.

Alban Mariette, Hee Sung Kang, Joshua L Heazlewood, Staffan Persson, Berit Ebert, Edwin R Lampugnani

2021-06-15

Growth, development, structure as well as dynamic adaptations and remodelling processes in plants are largely controlled by the pr..

An Arabidopsis lipid map reveals differences between tissues and dynamic changes throughout development

Cheka Kehelpannala, Thusitha Rupasinghe, Asher Pasha, Eddi Esteban, Thomas Hennessy, David Bradley, Berit Ebert, Nicholas J Provart, Ute Roessner

2021-05-24

Mass spectrometry is the predominant analytical tool used in the field of plant lipidomics. However, there are many challenges ass..

A DUF1068 protein acts as a pectin biosynthesis scaffold and maintains Golgi morphology and cell adhesion in Arabidopsis

Rahul Lathe, Heather McFarlane, Ghazanfar Abbas Khan, Berit Ebert, Eduardo Antonio Ramírez-Rodríguez, Niels Noord, Rishikesh Bhalerao, Staffan Persson

2021-05-03

<h4>ABSTRACT</h4> Adjacent plant cells are connected by specialized cell wall regions, called middle lamellae, which influence cri..

A Pipeline towards the Biochemical Characterization of the Arabidopsis GT14 Family

Lingling Xuan, Jie Zhang, Weitai Lu, Pawel Gluza, Berit Ebert, Toshihisa Kotake, Mengzhu Lu, Yuan Zhang, Mads H Clausen, Kim L Johnson, Monika S Doblin, Joshua L Heazlewood, Antony Bacic, Lili Song, Wei Zeng

2021-02-01

Glycosyltransferases (GTs) catalyze the synthesis of glycosidic linkages and are essential in the biosynthesis of glycans, glycoco..

A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues

Cheka Kehelpannala, Thusitha WT Rupasinghe, Thomas Hennessy, David Bradley, Berit Ebert, Ute Roessner

2020-12-03

Background The plant lipidome is highly complex, and the composition of lipids in different tissues as well as their specific func..

UDP-Api/UDP-Xyl synthases affect plant development by controlling the content of UDP-Api to regulate the RG-II-borate complex

Xianhai Zhao, Berit Ebert, Baocai Zhang, Huabin Liu, Yutao Zhang, Wei Zeng, Carsten Rautengarten, Huiling Li, Xiaoyang Chen, Antony Bacic, Guodong Wang, Shuzhen Men, Yihua Zhou, Joshua L Heazlewood, Ai-Min Wu

2020-09-01

Rhamnogalacturonan‐II (RG‐II) is structurally the most complex glycan in higher plants, containing 13 different sugars and 21 dist..

Conserved Glu-47 and Lys-50 residues are critical for UDP-N-acetylglucosamine/UMP antiport activity of the mouse Golgi-associated transporter Slc35a3

M Agustina Toscanini, M Belen Favarolo, F Luis Gonzalez Flecha, Berit Ebert, Carsten Rautengarten, Luis M Bredeston

2019-06-28

Nucleotide sugar transporters (NSTs) regulate the flux of activated sugars from the cytosol into the lumen of the Golgi apparatus ..

Profiling Cell Wall Monosaccharides and Nucleotide-Sugars from Plants.

Carsten Rautengarten, Joshua L Heazlewood, Berit Ebert

2019-06-01

The cell wall is an intricate mesh largely composed of polysaccharides that vary in structure and abundance. Apart from cellulose ..

The Three Members of the Arabidopsis Glycosyltransferase Family 92 are Functional beta-1,4-Galactan Synthases

Berit Ebert, Devon Birdseye, April JM Liwanag, Tomas Laursen, Emilie A Rennie, Xiaoyuan Guo, Michela Catena, Carsten Rautengarten, Solomon H Stonebloom, Pawel Gluza, Venkataramana R Pidatala, Mathias CF Andersen, Roshan Cheetamun, Jenny C Mortimer, Joshua L Heazlewood, Antony Bacic, Mads H Clausen, William GT Willats, Henrik V Scheller

2018-12-01

Pectin is a major component of primary cell walls and performs a plethora of functions crucial for plant growth, development and p..

A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids

Berit Ebert, Carsten Rautengarten, Heather E McFarlane, Thusitha Rupasinghe, Wei Zeng, Kristina Ford, Henrik V Scheller, Antony Bacic, Ute Roessner, Staffan Persson, Joshua L Heazlewood

2018-10-01

Glycosylation requires activated glycosyl donors in the form of nucleotide sugars to drive processes such as post-translational pr..

Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis

Xianhai Zhao, Nian Liu, Na Shang, Wei Zeng, Berit Ebert, Carsten Rautengarten, Qing-Yin Zeng, Huiling Li, Xiaoyang Chen, Cherie Beahan, Antony Bacic, Joshua L Heazlewood, Ai-Min Wu

2018-02-20

UDP-xylose (UDP-Xyl) is synthesized by UDP-glucuronic acid decarboxylases, also termed UDP-Xyl synthases (UXSs). The Arabidopsis g..

Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters.

Carsten Rautengarten, Berit Ebert, Joshua L Heazlewood

2018-01-01

The purification of a functional soluble protein from biological or in vitro expression systems can be problematic and the enrichm..

The elaborate route for UDP-arabinose delivery into the Golgi of plants

Carsten Rautengarten, Devon Birdseye, Sivakumar Pattathil, Heather E McFarlane, Susana Saez-Aguayo, Ariel Orellana, Staffan Persson, Michael G Hahn, Henrik V Scheller, Joshua L Heazlewood, Berit Ebert

2017-04-18

In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptide..