Surveillance of the mechanisms controlling proteome foldedness
Grant number: DP170103093 | Funding period: 2017 - 2020
This project aims to measure how cells keep the proteome folded. Cells have extensive quality control networks to govern synthesis, folding and transport of every protein but the buffering capacity of this system is not definable. This capacity is needed to understand how problems arise in managing proteome foldedness, a central feature of human diseases and biotechnology and synthetic biology applications that need cell-based production of engineered proteins such as hormones and antibodies. The outcomes are expected to provide basic knowledge of this fundamental process and provide biosensors and screening methods for use in health and biotechnology industries.
Related publications (8)
Immiscible inclusion bodies formed by polyglutamine and poly(glycine-alanine) are enriched with distinct proteomes but converge in proteins that are risk factors for disease and involved in protein degradation
Mona Radwan, Jordan D Lilley, Ching-Seng Ang, Gavin E Reid, Danny M Hatters
Poly(glycine-alanine) (polyGA) is one of the polydipeptides expressed in Frontotemporal Dementia and/or Amyotrophic Lateral Sclero..
Inclusion bodies formed by polyglutamine and poly(glycine-alanine) are enriched with distinct proteomes but converge in proteins that are risk factors for disease and involved in protein degradation
Mona Radwan, Jordan Lilley, Ching-Seng Ang, Gavin Reid, Danny Hatters
<h4>ABSTRACT</h4> Poly(glycine-alanine) (polyGA) is one of the dipolypeptides expressed in Motor Neuron Disease caused by C9ORF72 ..
Arginine in C9ORF72 Dipolypeptides Mediates Promiscuous Proteome Binding and Multiple Modes of Toxicity
Mona Radwan, Ching-Seng Ang, Angelique R Ormsby, Dezerae Cox, James C Daly, Gavin E Reid, Danny M Hatters
C9ORF72-associated Motor Neuron Disease patients feature abnormal expression of 5 dipeptide repeat (DPR) polymers. Here we used qu..
Widespread remodeling of proteome solubility in response to different protein homeostasis stresses
Xiaojing Sui, Douglas EV Pires, Angelique R Ormsby, Dezerae Cox, Shuai Nie, Giulia Vecchi, Michele Vendruscolo, David B Ascher, Gavin E Reid, Danny M Hatters
The accumulation of protein deposits in neurodegenerative diseases has been hypothesized to depend on a metastable subproteome vul..
Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal
Lorena Hidalgo San Jose, Mary Jean Sunshine, Christopher H Dillingham, Bernadette A Chua, Miriama Kruta, Yuning Hong, Danny M Hatters, Robert AJ Signer
Low protein synthesis is a feature of somatic stem cells that promotes regeneration in multiple tissues. Modest increases in prote..
A thiol probe for measuring unfolded protein load and proteostasis in cells
Moore Z Chen, Nagaraj S Moily, Jessica L Bridgford, Rebecca J Wood, Mona Radwan, Trevor A Smith, Zhegang Song, Ben Zhong Tang, Leann Tilley, Xiaohong Xu, Gavin E Reid, Mahmoud A Pouladi, Yuning Hong, Danny M Hatters
When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethe..