Surveillance of the mechanisms controlling proteome foldedness

Grant number: DP170103093 | Funding period: 2017 - 2020

Completed

Abstract

This project aims to measure how cells keep the proteome folded. Cells have extensive quality control networks to govern synthesis, folding and transport of every protein but the buffering capacity of this system is not definable. This capacity is needed to understand how problems arise in managing proteome foldedness, a central feature of human diseases and biotechnology and synthetic biology applications that need cell-based production of engineered proteins such as hormones and antibodies. The outcomes are expected to provide basic knowledge of this fundamental process and provide biosensors and screening methods for use in health and biotechnology industries.

Related publications (8)

Scholarly works icon

Immiscible inclusion bodies formed by polyglutamine and poly(glycine-alanine) are enriched with distinct proteomes but converge in proteins that are risk factors for disease and involved in protein degradation

Mona Radwan, Jordan D Lilley, Ching-Seng Ang, Gavin E Reid, Danny M Hatters

2020-08-28

Poly(glycine-alanine) (polyGA) is one of the polydipeptides expressed in Frontotemporal Dementia and/or Amyotrophic Lateral Sclero..

Scholarly works icon

Inclusion bodies formed by polyglutamine and poly(glycine-alanine) are enriched with distinct proteomes but converge in proteins that are risk factors for disease and involved in protein degradation

Mona Radwan, Jordan Lilley, Ching-Seng Ang, Gavin Reid, Danny Hatters

2020-05-04

<h4>ABSTRACT</h4> Poly(glycine-alanine) (polyGA) is one of the dipolypeptides expressed in Motor Neuron Disease caused by C9ORF72 ..

Scholarly works icon

Arginine in C9ORF72 Dipolypeptides Mediates Promiscuous Proteome Binding and Multiple Modes of Toxicity

Mona Radwan, Ching-Seng Ang, Angelique R Ormsby, Dezerae Cox, James C Daly, Gavin E Reid, Danny M Hatters

2020-04-01

C9ORF72-associated Motor Neuron Disease patients feature abnormal expression of 5 dipeptide repeat (DPR) polymers. Here we used qu..

Scholarly works icon

Protein aggregation in cell biology: An aggregomics perspective of health and disease

Dezerae Cox, Candice Raeburn, Xiaojing Sui, Danny M Hatters

2020-03-01

Maintaining protein homeostasis (proteostasis) is essential for cellular health and is governed by a network of quality control ma..

Scholarly works icon

Widespread remodeling of proteome solubility in response to different protein homeostasis stresses

Xiaojing Sui, Douglas EV Pires, Angelique R Ormsby, Dezerae Cox, Shuai Nie, Giulia Vecchi, Michele Vendruscolo, David B Ascher, Gavin E Reid, Danny M Hatters

2020-02-04

The accumulation of protein deposits in neurodegenerative diseases has been hypothesized to depend on a metastable subproteome vul..

Scholarly works icon

Modest Declines in Proteome Quality Impair Hematopoietic Stem Cell Self-Renewal

Lorena Hidalgo San Jose, Mary Jean Sunshine, Christopher H Dillingham, Bernadette A Chua, Miriama Kruta, Yuning Hong, Danny M Hatters, Robert AJ Signer

2020-01-07

Low protein synthesis is a feature of somatic stem cells that promotes regeneration in multiple tissues. Modest increases in prote..

Scholarly works icon

A thiol probe for measuring unfolded protein load and proteostasis in cells

Moore Z Chen, Nagaraj S Moily, Jessica L Bridgford, Rebecca J Wood, Mona Radwan, Trevor A Smith, Zhegang Song, Ben Zhong Tang, Leann Tilley, Xiaohong Xu, Gavin E Reid, Mahmoud A Pouladi, Yuning Hong, Danny M Hatters

2017-09-07

When proteostasis becomes unbalanced, unfolded proteins can accumulate and aggregate. Here we report that the dye, tetraphenylethe..

Scholarly works icon

When Proteostasis Goes Bad: Protein Aggregation in the Cell

Mona Radwan, Rebecca J Wood, Xiaojing Sui, Danny M Hatters

2017-02-01

Protein aggregation is a hallmark of the major neurodegenerative diseases including Alzheimer's, Parkinson's, Huntington's and mot..

University of Melbourne Researchers