MULTIPLEXED BIO-IMAGING MASS SPECTROMETRY

Grant number: DP170100036 | Funding period: 2017 - 2020

Completed

Abstract

This project aims to develop technology for simultaneous three-dimensional imaging of trace elements and molecules in biological tissues. This technology is an essential analytical and diagnostic advance to uncover latent mechanistic biology and will reveal subtle metabolic interactions between cells. The project will use laser ablation-inductively coupled plasma-mass spectrometry and lanthanide-conjugated probes to map the zinc metabolic pathway in the ageing mouse brain. It is expected to result in a complete imaging solution for quantitative spatial determination of all important elements, co-factors, metabolites and other functional molecules to investigate metabolic pathways which curre..

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Related publications (4)

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Matching sensitivity to abundance: high resolution immuno-mass spectrometry imaging of lanthanide labels and endogenous elements in the murine brain

David Clases, Raquel Gonzalez de Vega, Sabrina Funke, Thomas E Lockwood, Mika T Westerhausen, R Verena Taudte, Paul A Adlard, Philip A Doble

2020-04-01

This work introduces a new method for immuno-mass spectrometry imaging via quadrupole-based laser ablation-inductively coupled pla..

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Characterising the spatial and temporal brain metal profile in a mouse model of tauopathy

Shalini S Rao, Larissa Lago, Raquel Gonzalez de Vega, Lisa Bray, Dominic J Hare, David Clases, Philip A Doble, Paul A Adlard

2020-02-01

A dysregulation in the homeostasis of metals such as copper, iron and zinc is speculated to be involved in the pathogenesis of tau..

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Dietary zinc and the control of Streptococcus pneumoniae infection

Bart A Eijkelkamp, Jacqueline R Morey, Stephanie L Neville, Aimee Tan, Victoria G Pederick, Nerida Cole, Prashina P Singh, Cheryl-Lynn Y Ong, Raquel Gonzalez de Vega, David Clases, Bliss A Cunningham, Catherine E Hughes, Iain Comerford, Erin B Brazel, Jonathan J Whittall, Charles D Plumptre, Shaun R McColl, James C Paton, Alastair G McEwan, Philip A Doble

2019-08-01

Human zinc deficiency increases susceptibility to bacterial infection. Although zinc supplementation therapies can reduce the impa..

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On-line reverse isotope dilution analysis for spatial quantification of elemental labels used in immunohistochemical assisted imaging mass spectrometry via LA-ICP-MS

David Clases, Raquel Gonzalez de Vega, Paul A Adlard, Philip A Doble

2019-02-01

We present a novel on-line isotope dilution analysis (IDA) approach for the quantification of isotopically enriched metal labels u..